Job ID = 9158335 sra ファイルのダウンロード中... Completed: 711326K bytes transferred in 9 seconds (594106K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 5177634 spots for /home/okishinya/chipatlas/results/dm3/SRX113305/SRR392911.sra Written 5177634 spots total Written 17982328 spots for /home/okishinya/chipatlas/results/dm3/SRX113305/SRR392910.sra Written 17982328 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 23159962 reads; of these: 23159962 (100.00%) were unpaired; of these: 1153997 (4.98%) aligned 0 times 17720772 (76.51%) aligned exactly 1 time 4285193 (18.50%) aligned >1 times 95.02% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 4717404 / 22005965 = 0.2144 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:22:48: # Command line: callpeak -t SRX113305.bam -f BAM -g dm -n SRX113305.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX113305.05 # format = BAM # ChIP-seq file = ['SRX113305.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:22:48: # Command line: callpeak -t SRX113305.bam -f BAM -g dm -n SRX113305.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX113305.10 # format = BAM # ChIP-seq file = ['SRX113305.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:22:48: # Command line: callpeak -t SRX113305.bam -f BAM -g dm -n SRX113305.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX113305.20 # format = BAM # ChIP-seq file = ['SRX113305.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:22:48: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:22:48: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:22:48: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:22:48: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:22:48: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:22:48: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:22:54: 1000000 INFO @ Tue, 27 Jun 2017 16:22:55: 1000000 INFO @ Tue, 27 Jun 2017 16:22:55: 1000000 INFO @ Tue, 27 Jun 2017 16:23:01: 2000000 INFO @ Tue, 27 Jun 2017 16:23:01: 2000000 INFO @ Tue, 27 Jun 2017 16:23:01: 2000000 INFO @ Tue, 27 Jun 2017 16:23:07: 3000000 INFO @ Tue, 27 Jun 2017 16:23:08: 3000000 INFO @ Tue, 27 Jun 2017 16:23:08: 3000000 INFO @ Tue, 27 Jun 2017 16:23:13: 4000000 INFO @ Tue, 27 Jun 2017 16:23:15: 4000000 INFO @ Tue, 27 Jun 2017 16:23:15: 4000000 INFO @ Tue, 27 Jun 2017 16:23:20: 5000000 INFO @ Tue, 27 Jun 2017 16:23:22: 5000000 INFO @ Tue, 27 Jun 2017 16:23:22: 5000000 INFO @ Tue, 27 Jun 2017 16:23:26: 6000000 INFO @ Tue, 27 Jun 2017 16:23:28: 6000000 INFO @ Tue, 27 Jun 2017 16:23:28: 6000000 INFO @ Tue, 27 Jun 2017 16:23:32: 7000000 INFO @ Tue, 27 Jun 2017 16:23:35: 7000000 INFO @ Tue, 27 Jun 2017 16:23:35: 7000000 INFO @ Tue, 27 Jun 2017 16:23:39: 8000000 INFO @ Tue, 27 Jun 2017 16:23:41: 8000000 INFO @ Tue, 27 Jun 2017 16:23:41: 8000000 INFO @ Tue, 27 Jun 2017 16:23:45: 9000000 INFO @ Tue, 27 Jun 2017 16:23:48: 9000000 INFO @ Tue, 27 Jun 2017 16:23:48: 9000000 INFO @ Tue, 27 Jun 2017 16:23:52: 10000000 INFO @ Tue, 27 Jun 2017 16:23:55: 10000000 INFO @ Tue, 27 Jun 2017 16:23:55: 10000000 INFO @ Tue, 27 Jun 2017 16:23:58: 11000000 INFO @ Tue, 27 Jun 2017 16:24:01: 11000000 INFO @ Tue, 27 Jun 2017 16:24:01: 11000000 INFO @ Tue, 27 Jun 2017 16:24:04: 12000000 INFO @ Tue, 27 Jun 2017 16:24:08: 12000000 INFO @ Tue, 27 Jun 2017 16:24:08: 12000000 INFO @ Tue, 27 Jun 2017 16:24:11: 13000000 INFO @ Tue, 27 Jun 2017 16:24:14: 13000000 INFO @ Tue, 27 Jun 2017 16:24:14: 13000000 INFO @ Tue, 27 Jun 2017 16:24:17: 14000000 INFO @ Tue, 27 Jun 2017 16:24:21: 14000000 INFO @ Tue, 27 Jun 2017 16:24:21: 14000000 INFO @ Tue, 27 Jun 2017 16:24:24: 15000000 INFO @ Tue, 27 Jun 2017 16:24:28: 15000000 INFO @ Tue, 27 Jun 2017 16:24:28: 15000000 INFO @ Tue, 27 Jun 2017 16:24:30: 16000000 INFO @ Tue, 27 Jun 2017 16:24:34: 16000000 INFO @ Tue, 27 Jun 2017 16:24:34: 16000000 INFO @ Tue, 27 Jun 2017 16:24:36: 17000000 INFO @ Tue, 27 Jun 2017 16:24:38: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:24:38: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:24:38: #1 total tags in treatment: 17288561 INFO @ Tue, 27 Jun 2017 16:24:38: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:24:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:24:38: #1 tags after filtering in treatment: 17288561 INFO @ Tue, 27 Jun 2017 16:24:38: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:24:38: #1 finished! INFO @ Tue, 27 Jun 2017 16:24:38: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:24:38: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:24:40: #2 number of paired peaks: 50 WARNING @ Tue, 27 Jun 2017 16:24:40: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:24:40: Process for pairing-model is terminated! cat: SRX113305.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113305.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113305.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113305.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:24:41: 17000000 INFO @ Tue, 27 Jun 2017 16:24:41: 17000000 INFO @ Tue, 27 Jun 2017 16:24:43: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:24:43: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:24:43: #1 total tags in treatment: 17288561 INFO @ Tue, 27 Jun 2017 16:24:43: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:24:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:24:43: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:24:43: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:24:43: #1 total tags in treatment: 17288561 INFO @ Tue, 27 Jun 2017 16:24:43: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:24:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:24:43: #1 tags after filtering in treatment: 17288561 INFO @ Tue, 27 Jun 2017 16:24:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:24:43: #1 finished! INFO @ Tue, 27 Jun 2017 16:24:43: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:24:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:24:43: #1 tags after filtering in treatment: 17288561 INFO @ Tue, 27 Jun 2017 16:24:43: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:24:43: #1 finished! INFO @ Tue, 27 Jun 2017 16:24:43: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:24:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:24:44: #2 number of paired peaks: 50 WARNING @ Tue, 27 Jun 2017 16:24:44: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:24:44: Process for pairing-model is terminated! cat: SRX113305.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113305.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113305.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113305.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:24:44: #2 number of paired peaks: 50 WARNING @ Tue, 27 Jun 2017 16:24:44: Too few paired peaks (50) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:24:44: Process for pairing-model is terminated! cat: SRX113305.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX113305.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113305.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX113305.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。