Job ID = 16439425 SRX = SRX11245082 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T06:08:07 prefetch.2.10.7: 1) Downloading 'SRR14932347'... 2022-08-02T06:08:07 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T06:09:13 prefetch.2.10.7: HTTPS download succeed 2022-08-02T06:09:13 prefetch.2.10.7: 1) 'SRR14932347' was downloaded successfully 2022-08-02T06:09:13 prefetch.2.10.7: 'SRR14932347' has 0 unresolved dependencies Read 28796094 spots for SRR14932347/SRR14932347.sra Written 28796094 spots for SRR14932347/SRR14932347.sra fastq に変換しました。 bowtie でマッピング中... Your job 16441090 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 03:16:59 28796094 reads; of these: 28796094 (100.00%) were paired; of these: 7068374 (24.55%) aligned concordantly 0 times 15729381 (54.62%) aligned concordantly exactly 1 time 5998339 (20.83%) aligned concordantly >1 times ---- 7068374 pairs aligned concordantly 0 times; of these: 2632165 (37.24%) aligned discordantly 1 time ---- 4436209 pairs aligned 0 times concordantly or discordantly; of these: 8872418 mates make up the pairs; of these: 6154940 (69.37%) aligned 0 times 734271 (8.28%) aligned exactly 1 time 1983207 (22.35%) aligned >1 times 89.31% overall alignment rate Time searching: 03:16:59 Overall time: 03:16:59 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7591038 / 24266920 = 0.3128 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:57:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:57:29: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:57:29: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:57:43: 1000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:57:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:57:57: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:57:57: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:57:57: 2000000 INFO @ Tue, 02 Aug 2022 18:58:12: 3000000 INFO @ Tue, 02 Aug 2022 18:58:13: 1000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:58:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:58:26: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:58:26: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:58:28: 4000000 INFO @ Tue, 02 Aug 2022 18:58:30: 2000000 INFO @ Tue, 02 Aug 2022 18:58:42: 1000000 INFO @ Tue, 02 Aug 2022 18:58:44: 5000000 INFO @ Tue, 02 Aug 2022 18:58:46: 3000000 INFO @ Tue, 02 Aug 2022 18:58:55: 2000000 INFO @ Tue, 02 Aug 2022 18:58:59: 6000000 INFO @ Tue, 02 Aug 2022 18:59:03: 4000000 INFO @ Tue, 02 Aug 2022 18:59:08: 3000000 INFO @ Tue, 02 Aug 2022 18:59:16: 7000000 INFO @ Tue, 02 Aug 2022 18:59:19: 5000000 INFO @ Tue, 02 Aug 2022 18:59:22: 4000000 INFO @ Tue, 02 Aug 2022 18:59:32: 8000000 INFO @ Tue, 02 Aug 2022 18:59:35: 6000000 INFO @ Tue, 02 Aug 2022 18:59:36: 5000000 INFO @ Tue, 02 Aug 2022 18:59:47: 9000000 INFO @ Tue, 02 Aug 2022 18:59:49: 6000000 INFO @ Tue, 02 Aug 2022 18:59:51: 7000000 INFO @ Tue, 02 Aug 2022 19:00:03: 10000000 INFO @ Tue, 02 Aug 2022 19:00:03: 7000000 INFO @ Tue, 02 Aug 2022 19:00:08: 8000000 INFO @ Tue, 02 Aug 2022 19:00:17: 8000000 INFO @ Tue, 02 Aug 2022 19:00:19: 11000000 INFO @ Tue, 02 Aug 2022 19:00:24: 9000000 INFO @ Tue, 02 Aug 2022 19:00:30: 9000000 INFO @ Tue, 02 Aug 2022 19:00:34: 12000000 INFO @ Tue, 02 Aug 2022 19:00:40: 10000000 INFO @ Tue, 02 Aug 2022 19:00:43: 10000000 INFO @ Tue, 02 Aug 2022 19:00:52: 13000000 INFO @ Tue, 02 Aug 2022 19:00:55: 11000000 INFO @ Tue, 02 Aug 2022 19:00:56: 11000000 INFO @ Tue, 02 Aug 2022 19:01:08: 14000000 INFO @ Tue, 02 Aug 2022 19:01:11: 12000000 INFO @ Tue, 02 Aug 2022 19:01:11: 12000000 INFO @ Tue, 02 Aug 2022 19:01:24: 15000000 INFO @ Tue, 02 Aug 2022 19:01:24: 13000000 INFO @ Tue, 02 Aug 2022 19:01:28: 13000000 INFO @ Tue, 02 Aug 2022 19:01:38: 14000000 INFO @ Tue, 02 Aug 2022 19:01:38: 16000000 INFO @ Tue, 02 Aug 2022 19:01:45: 14000000 INFO @ Tue, 02 Aug 2022 19:01:51: 15000000 INFO @ Tue, 02 Aug 2022 19:01:51: 17000000 INFO @ Tue, 02 Aug 2022 19:02:00: 15000000 INFO @ Tue, 02 Aug 2022 19:02:03: 16000000 INFO @ Tue, 02 Aug 2022 19:02:05: 18000000 INFO @ Tue, 02 Aug 2022 19:02:16: 17000000 INFO @ Tue, 02 Aug 2022 19:02:16: 16000000 INFO @ Tue, 02 Aug 2022 19:02:17: 19000000 INFO @ Tue, 02 Aug 2022 19:02:29: 18000000 INFO @ Tue, 02 Aug 2022 19:02:29: 20000000 INFO @ Tue, 02 Aug 2022 19:02:32: 17000000 INFO @ Tue, 02 Aug 2022 19:02:42: 19000000 INFO @ Tue, 02 Aug 2022 19:02:43: 21000000 INFO @ Tue, 02 Aug 2022 19:02:48: 18000000 INFO @ Tue, 02 Aug 2022 19:02:54: 20000000 INFO @ Tue, 02 Aug 2022 19:02:55: 22000000 INFO @ Tue, 02 Aug 2022 19:03:04: 19000000 INFO @ Tue, 02 Aug 2022 19:03:07: 21000000 INFO @ Tue, 02 Aug 2022 19:03:09: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 19:03:20: 22000000 INFO @ Tue, 02 Aug 2022 19:03:20: 20000000 INFO @ Tue, 02 Aug 2022 19:03:22: 24000000 INFO @ Tue, 02 Aug 2022 19:03:32: 23000000 INFO @ Tue, 02 Aug 2022 19:03:35: 21000000 INFO @ Tue, 02 Aug 2022 19:03:36: 25000000 INFO @ Tue, 02 Aug 2022 19:03:45: 24000000 INFO @ Tue, 02 Aug 2022 19:03:49: 26000000 INFO @ Tue, 02 Aug 2022 19:03:51: 22000000 INFO @ Tue, 02 Aug 2022 19:03:58: 25000000 INFO @ Tue, 02 Aug 2022 19:04:04: 27000000 INFO @ Tue, 02 Aug 2022 19:04:06: 23000000 INFO @ Tue, 02 Aug 2022 19:04:11: 26000000 INFO @ Tue, 02 Aug 2022 19:04:18: 28000000 INFO @ Tue, 02 Aug 2022 19:04:22: 24000000 INFO @ Tue, 02 Aug 2022 19:04:26: 27000000 INFO @ Tue, 02 Aug 2022 19:04:33: 29000000 INFO @ Tue, 02 Aug 2022 19:04:38: 25000000 INFO @ Tue, 02 Aug 2022 19:04:39: 28000000 INFO @ Tue, 02 Aug 2022 19:04:48: 30000000 INFO @ Tue, 02 Aug 2022 19:04:53: 29000000 INFO @ Tue, 02 Aug 2022 19:04:55: 26000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 19:05:03: 31000000 INFO @ Tue, 02 Aug 2022 19:05:08: 30000000 INFO @ Tue, 02 Aug 2022 19:05:11: 27000000 INFO @ Tue, 02 Aug 2022 19:05:20: 32000000 INFO @ Tue, 02 Aug 2022 19:05:23: 31000000 INFO @ Tue, 02 Aug 2022 19:05:27: 28000000 INFO @ Tue, 02 Aug 2022 19:05:38: 33000000 INFO @ Tue, 02 Aug 2022 19:05:39: 32000000 INFO @ Tue, 02 Aug 2022 19:05:43: 29000000 INFO @ Tue, 02 Aug 2022 19:05:55: 34000000 INFO @ Tue, 02 Aug 2022 19:05:57: 33000000 INFO @ Tue, 02 Aug 2022 19:05:58: 30000000 INFO @ Tue, 02 Aug 2022 19:06:12: 35000000 INFO @ Tue, 02 Aug 2022 19:06:14: 34000000 INFO @ Tue, 02 Aug 2022 19:06:14: 31000000 INFO @ Tue, 02 Aug 2022 19:06:27: 36000000 INFO @ Tue, 02 Aug 2022 19:06:31: 32000000 INFO @ Tue, 02 Aug 2022 19:06:31: 35000000 INFO @ Tue, 02 Aug 2022 19:06:31: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 19:06:31: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 19:06:31: #1 total tags in treatment: 14988100 INFO @ Tue, 02 Aug 2022 19:06:31: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:06:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:06:32: #1 tags after filtering in treatment: 13937582 INFO @ Tue, 02 Aug 2022 19:06:32: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 19:06:32: #1 finished! INFO @ Tue, 02 Aug 2022 19:06:32: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:06:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:06:33: #2 number of paired peaks: 164 WARNING @ Tue, 02 Aug 2022 19:06:33: Fewer paired peaks (164) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 164 pairs to build model! INFO @ Tue, 02 Aug 2022 19:06:33: start model_add_line... INFO @ Tue, 02 Aug 2022 19:06:33: start X-correlation... INFO @ Tue, 02 Aug 2022 19:06:33: end of X-cor INFO @ Tue, 02 Aug 2022 19:06:33: #2 finished! INFO @ Tue, 02 Aug 2022 19:06:33: #2 predicted fragment length is 197 bps INFO @ Tue, 02 Aug 2022 19:06:33: #2 alternative fragment length(s) may be 197 bps INFO @ Tue, 02 Aug 2022 19:06:33: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.05_model.r WARNING @ Tue, 02 Aug 2022 19:06:33: #2 Since the d (197) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:06:33: #2 You may need to consider one of the other alternative d(s): 197 WARNING @ Tue, 02 Aug 2022 19:06:33: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:06:33: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:06:33: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:06:47: 36000000 INFO @ Tue, 02 Aug 2022 19:06:48: 33000000 INFO @ Tue, 02 Aug 2022 19:06:51: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 19:06:51: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 19:06:51: #1 total tags in treatment: 14988100 INFO @ Tue, 02 Aug 2022 19:06:51: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:06:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:06:52: #1 tags after filtering in treatment: 13937582 INFO @ Tue, 02 Aug 2022 19:06:52: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 19:06:52: #1 finished! INFO @ Tue, 02 Aug 2022 19:06:52: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:06:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:06:53: #2 number of paired peaks: 164 WARNING @ Tue, 02 Aug 2022 19:06:53: Fewer paired peaks (164) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 164 pairs to build model! INFO @ Tue, 02 Aug 2022 19:06:53: start model_add_line... INFO @ Tue, 02 Aug 2022 19:06:53: start X-correlation... INFO @ Tue, 02 Aug 2022 19:06:53: end of X-cor INFO @ Tue, 02 Aug 2022 19:06:53: #2 finished! INFO @ Tue, 02 Aug 2022 19:06:53: #2 predicted fragment length is 197 bps INFO @ Tue, 02 Aug 2022 19:06:53: #2 alternative fragment length(s) may be 197 bps INFO @ Tue, 02 Aug 2022 19:06:53: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.20_model.r WARNING @ Tue, 02 Aug 2022 19:06:53: #2 Since the d (197) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:06:53: #2 You may need to consider one of the other alternative d(s): 197 WARNING @ Tue, 02 Aug 2022 19:06:53: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:06:53: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:06:53: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:07:05: 34000000 INFO @ Tue, 02 Aug 2022 19:07:14: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:07:22: 35000000 INFO @ Tue, 02 Aug 2022 19:07:34: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:07:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.05_peaks.xls INFO @ Tue, 02 Aug 2022 19:07:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.05_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:07:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.05_summits.bed INFO @ Tue, 02 Aug 2022 19:07:37: Done! pass1 - making usageList (14 chroms): 3 millis pass2 - checking and writing primary data (5385 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:07:38: 36000000 INFO @ Tue, 02 Aug 2022 19:07:43: #1 tag size is determined as 150 bps INFO @ Tue, 02 Aug 2022 19:07:43: #1 tag size = 150 INFO @ Tue, 02 Aug 2022 19:07:43: #1 total tags in treatment: 14988100 INFO @ Tue, 02 Aug 2022 19:07:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 19:07:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 19:07:43: #1 tags after filtering in treatment: 13937582 INFO @ Tue, 02 Aug 2022 19:07:43: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 19:07:43: #1 finished! INFO @ Tue, 02 Aug 2022 19:07:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 19:07:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 19:07:44: #2 number of paired peaks: 164 WARNING @ Tue, 02 Aug 2022 19:07:44: Fewer paired peaks (164) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 164 pairs to build model! INFO @ Tue, 02 Aug 2022 19:07:44: start model_add_line... INFO @ Tue, 02 Aug 2022 19:07:44: start X-correlation... INFO @ Tue, 02 Aug 2022 19:07:44: end of X-cor INFO @ Tue, 02 Aug 2022 19:07:44: #2 finished! INFO @ Tue, 02 Aug 2022 19:07:44: #2 predicted fragment length is 197 bps INFO @ Tue, 02 Aug 2022 19:07:44: #2 alternative fragment length(s) may be 197 bps INFO @ Tue, 02 Aug 2022 19:07:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.10_model.r WARNING @ Tue, 02 Aug 2022 19:07:44: #2 Since the d (197) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Tue, 02 Aug 2022 19:07:44: #2 You may need to consider one of the other alternative d(s): 197 WARNING @ Tue, 02 Aug 2022 19:07:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Tue, 02 Aug 2022 19:07:44: #3 Call peaks... INFO @ Tue, 02 Aug 2022 19:07:44: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 02 Aug 2022 19:07:56: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.20_peaks.xls INFO @ Tue, 02 Aug 2022 19:07:56: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.20_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:07:56: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.20_summits.bed INFO @ Tue, 02 Aug 2022 19:07:56: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1106 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 19:08:25: #3 Call peaks for each chromosome... INFO @ Tue, 02 Aug 2022 19:08:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.10_peaks.xls INFO @ Tue, 02 Aug 2022 19:08:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.10_peaks.narrowPeak INFO @ Tue, 02 Aug 2022 19:08:47: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11245082/SRX11245082.10_summits.bed INFO @ Tue, 02 Aug 2022 19:08:47: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (2826 records, 4 fields): 11 millis CompletedMACS2peakCalling