Job ID = 9158318 sra ファイルのダウンロード中... Completed: 1412128K bytes transferred in 14 seconds (787629K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 11602926 spots for /home/okishinya/chipatlas/results/dm3/SRX111807/SRR390266.sra Written 11602926 spots total Written 34712355 spots for /home/okishinya/chipatlas/results/dm3/SRX111807/SRR390267.sra Written 34712355 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:13:49 46315281 reads; of these: 46315281 (100.00%) were unpaired; of these: 2563979 (5.54%) aligned 0 times 36376341 (78.54%) aligned exactly 1 time 7374961 (15.92%) aligned >1 times 94.46% overall alignment rate Time searching: 00:13:50 Overall time: 00:13:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 13963064 / 43751302 = 0.3191 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:26:43: # Command line: callpeak -t SRX111807.bam -f BAM -g dm -n SRX111807.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX111807.20 # format = BAM # ChIP-seq file = ['SRX111807.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:26:43: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:26:43: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:26:43: # Command line: callpeak -t SRX111807.bam -f BAM -g dm -n SRX111807.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX111807.05 # format = BAM # ChIP-seq file = ['SRX111807.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:26:43: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:26:43: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:26:43: # Command line: callpeak -t SRX111807.bam -f BAM -g dm -n SRX111807.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX111807.10 # format = BAM # ChIP-seq file = ['SRX111807.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:26:43: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:26:43: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:26:51: 1000000 INFO @ Tue, 27 Jun 2017 16:26:52: 1000000 INFO @ Tue, 27 Jun 2017 16:26:52: 1000000 INFO @ Tue, 27 Jun 2017 16:26:59: 2000000 INFO @ Tue, 27 Jun 2017 16:27:01: 2000000 INFO @ Tue, 27 Jun 2017 16:27:01: 2000000 INFO @ Tue, 27 Jun 2017 16:27:07: 3000000 INFO @ Tue, 27 Jun 2017 16:27:10: 3000000 INFO @ Tue, 27 Jun 2017 16:27:10: 3000000 INFO @ Tue, 27 Jun 2017 16:27:15: 4000000 INFO @ Tue, 27 Jun 2017 16:27:19: 4000000 INFO @ Tue, 27 Jun 2017 16:27:19: 4000000 INFO @ Tue, 27 Jun 2017 16:27:23: 5000000 INFO @ Tue, 27 Jun 2017 16:27:28: 5000000 INFO @ Tue, 27 Jun 2017 16:27:28: 5000000 INFO @ Tue, 27 Jun 2017 16:27:31: 6000000 INFO @ Tue, 27 Jun 2017 16:27:37: 6000000 INFO @ Tue, 27 Jun 2017 16:27:37: 6000000 INFO @ Tue, 27 Jun 2017 16:27:38: 7000000 INFO @ Tue, 27 Jun 2017 16:27:46: 7000000 INFO @ Tue, 27 Jun 2017 16:27:46: 7000000 INFO @ Tue, 27 Jun 2017 16:27:46: 8000000 INFO @ Tue, 27 Jun 2017 16:27:54: 9000000 INFO @ Tue, 27 Jun 2017 16:27:55: 8000000 INFO @ Tue, 27 Jun 2017 16:27:55: 8000000 INFO @ Tue, 27 Jun 2017 16:28:02: 10000000 INFO @ Tue, 27 Jun 2017 16:28:04: 9000000 INFO @ Tue, 27 Jun 2017 16:28:04: 9000000 INFO @ Tue, 27 Jun 2017 16:28:10: 11000000 INFO @ Tue, 27 Jun 2017 16:28:13: 10000000 INFO @ Tue, 27 Jun 2017 16:28:13: 10000000 INFO @ Tue, 27 Jun 2017 16:28:18: 12000000 INFO @ Tue, 27 Jun 2017 16:28:22: 11000000 INFO @ Tue, 27 Jun 2017 16:28:22: 11000000 INFO @ Tue, 27 Jun 2017 16:28:26: 13000000 INFO @ Tue, 27 Jun 2017 16:28:31: 12000000 INFO @ Tue, 27 Jun 2017 16:28:31: 12000000 INFO @ Tue, 27 Jun 2017 16:28:34: 14000000 INFO @ Tue, 27 Jun 2017 16:28:40: 13000000 INFO @ Tue, 27 Jun 2017 16:28:40: 13000000 INFO @ Tue, 27 Jun 2017 16:28:42: 15000000 INFO @ Tue, 27 Jun 2017 16:28:49: 14000000 INFO @ Tue, 27 Jun 2017 16:28:49: 14000000 INFO @ Tue, 27 Jun 2017 16:28:50: 16000000 INFO @ Tue, 27 Jun 2017 16:28:58: 17000000 INFO @ Tue, 27 Jun 2017 16:28:58: 15000000 INFO @ Tue, 27 Jun 2017 16:28:58: 15000000 INFO @ Tue, 27 Jun 2017 16:29:05: 18000000 INFO @ Tue, 27 Jun 2017 16:29:07: 16000000 INFO @ Tue, 27 Jun 2017 16:29:07: 16000000 INFO @ Tue, 27 Jun 2017 16:29:13: 19000000 INFO @ Tue, 27 Jun 2017 16:29:16: 17000000 INFO @ Tue, 27 Jun 2017 16:29:16: 17000000 INFO @ Tue, 27 Jun 2017 16:29:21: 20000000 INFO @ Tue, 27 Jun 2017 16:29:25: 18000000 INFO @ Tue, 27 Jun 2017 16:29:25: 18000000 INFO @ Tue, 27 Jun 2017 16:29:29: 21000000 INFO @ Tue, 27 Jun 2017 16:29:34: 19000000 INFO @ Tue, 27 Jun 2017 16:29:34: 19000000 INFO @ Tue, 27 Jun 2017 16:29:37: 22000000 INFO @ Tue, 27 Jun 2017 16:29:43: 20000000 INFO @ Tue, 27 Jun 2017 16:29:43: 20000000 INFO @ Tue, 27 Jun 2017 16:29:45: 23000000 INFO @ Tue, 27 Jun 2017 16:29:52: 21000000 INFO @ Tue, 27 Jun 2017 16:29:52: 21000000 INFO @ Tue, 27 Jun 2017 16:29:53: 24000000 INFO @ Tue, 27 Jun 2017 16:30:01: 22000000 INFO @ Tue, 27 Jun 2017 16:30:01: 22000000 INFO @ Tue, 27 Jun 2017 16:30:01: 25000000 INFO @ Tue, 27 Jun 2017 16:30:09: 26000000 INFO @ Tue, 27 Jun 2017 16:30:10: 23000000 INFO @ Tue, 27 Jun 2017 16:30:10: 23000000 INFO @ Tue, 27 Jun 2017 16:30:17: 27000000 INFO @ Tue, 27 Jun 2017 16:30:19: 24000000 INFO @ Tue, 27 Jun 2017 16:30:19: 24000000 INFO @ Tue, 27 Jun 2017 16:30:25: 28000000 INFO @ Tue, 27 Jun 2017 16:30:29: 25000000 INFO @ Tue, 27 Jun 2017 16:30:29: 25000000 INFO @ Tue, 27 Jun 2017 16:30:33: 29000000 INFO @ Tue, 27 Jun 2017 16:30:38: 26000000 INFO @ Tue, 27 Jun 2017 16:30:38: 26000000 INFO @ Tue, 27 Jun 2017 16:30:39: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:30:39: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:30:39: #1 total tags in treatment: 29788238 INFO @ Tue, 27 Jun 2017 16:30:39: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:30:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:30:40: #1 tags after filtering in treatment: 29788238 INFO @ Tue, 27 Jun 2017 16:30:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:30:40: #1 finished! INFO @ Tue, 27 Jun 2017 16:30:40: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:30:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:30:42: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:30:42: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:30:42: Process for pairing-model is terminated! cat: SRX111807.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111807.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111807.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111807.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:30:47: 27000000 INFO @ Tue, 27 Jun 2017 16:30:47: 27000000 INFO @ Tue, 27 Jun 2017 16:30:56: 28000000 INFO @ Tue, 27 Jun 2017 16:30:56: 28000000 INFO @ Tue, 27 Jun 2017 16:31:05: 29000000 INFO @ Tue, 27 Jun 2017 16:31:05: 29000000 INFO @ Tue, 27 Jun 2017 16:31:12: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:31:12: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:31:12: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:31:12: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:31:12: #1 total tags in treatment: 29788238 INFO @ Tue, 27 Jun 2017 16:31:12: #1 total tags in treatment: 29788238 INFO @ Tue, 27 Jun 2017 16:31:12: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:31:12: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:31:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:31:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:31:13: #1 tags after filtering in treatment: 29788238 INFO @ Tue, 27 Jun 2017 16:31:13: #1 tags after filtering in treatment: 29788238 INFO @ Tue, 27 Jun 2017 16:31:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:31:13: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:31:13: #1 finished! INFO @ Tue, 27 Jun 2017 16:31:13: #1 finished! INFO @ Tue, 27 Jun 2017 16:31:13: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:31:13: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:31:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:31:13: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:31:15: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:31:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:31:15: Process for pairing-model is terminated! cat: SRX111807.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Tue, 27 Jun 2017 16:31:15: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:31:15: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:31:15: Process for pairing-model is terminated! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111807.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111807.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111807.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling cat: SRX111807.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111807.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111807.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111807.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。