Job ID = 1293721 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 16,315,027 reads read : 16,315,027 reads written : 16,315,027 spots read : 19,174,327 reads read : 19,174,327 reads written : 19,174,327 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:17:27 35489354 reads; of these: 35489354 (100.00%) were unpaired; of these: 1235293 (3.48%) aligned 0 times 16994826 (47.89%) aligned exactly 1 time 17259235 (48.63%) aligned >1 times 96.52% overall alignment rate Time searching: 00:17:28 Overall time: 00:17:28 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 13435430 / 34254061 = 0.3922 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:18:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:18:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:18:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:18:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:18:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:18:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:18:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:18:53: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:18:53: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:19:00: 1000000 INFO @ Mon, 03 Jun 2019 02:19:00: 1000000 INFO @ Mon, 03 Jun 2019 02:19:01: 1000000 INFO @ Mon, 03 Jun 2019 02:19:07: 2000000 INFO @ Mon, 03 Jun 2019 02:19:08: 2000000 INFO @ Mon, 03 Jun 2019 02:19:09: 2000000 INFO @ Mon, 03 Jun 2019 02:19:14: 3000000 INFO @ Mon, 03 Jun 2019 02:19:15: 3000000 INFO @ Mon, 03 Jun 2019 02:19:16: 3000000 INFO @ Mon, 03 Jun 2019 02:19:21: 4000000 INFO @ Mon, 03 Jun 2019 02:19:23: 4000000 INFO @ Mon, 03 Jun 2019 02:19:23: 4000000 INFO @ Mon, 03 Jun 2019 02:19:27: 5000000 INFO @ Mon, 03 Jun 2019 02:19:30: 5000000 INFO @ Mon, 03 Jun 2019 02:19:31: 5000000 INFO @ Mon, 03 Jun 2019 02:19:34: 6000000 INFO @ Mon, 03 Jun 2019 02:19:37: 6000000 INFO @ Mon, 03 Jun 2019 02:19:39: 6000000 INFO @ Mon, 03 Jun 2019 02:19:41: 7000000 INFO @ Mon, 03 Jun 2019 02:19:44: 7000000 INFO @ Mon, 03 Jun 2019 02:19:46: 7000000 INFO @ Mon, 03 Jun 2019 02:19:48: 8000000 INFO @ Mon, 03 Jun 2019 02:19:51: 8000000 INFO @ Mon, 03 Jun 2019 02:19:54: 8000000 INFO @ Mon, 03 Jun 2019 02:19:54: 9000000 INFO @ Mon, 03 Jun 2019 02:19:58: 9000000 INFO @ Mon, 03 Jun 2019 02:20:01: 10000000 INFO @ Mon, 03 Jun 2019 02:20:02: 9000000 INFO @ Mon, 03 Jun 2019 02:20:05: 10000000 INFO @ Mon, 03 Jun 2019 02:20:08: 11000000 INFO @ Mon, 03 Jun 2019 02:20:10: 10000000 INFO @ Mon, 03 Jun 2019 02:20:12: 11000000 INFO @ Mon, 03 Jun 2019 02:20:15: 12000000 INFO @ Mon, 03 Jun 2019 02:20:17: 11000000 INFO @ Mon, 03 Jun 2019 02:20:20: 12000000 INFO @ Mon, 03 Jun 2019 02:20:21: 13000000 INFO @ Mon, 03 Jun 2019 02:20:25: 12000000 INFO @ Mon, 03 Jun 2019 02:20:27: 13000000 INFO @ Mon, 03 Jun 2019 02:20:28: 14000000 INFO @ Mon, 03 Jun 2019 02:20:33: 13000000 INFO @ Mon, 03 Jun 2019 02:20:34: 14000000 INFO @ Mon, 03 Jun 2019 02:20:35: 15000000 INFO @ Mon, 03 Jun 2019 02:20:41: 14000000 INFO @ Mon, 03 Jun 2019 02:20:41: 15000000 INFO @ Mon, 03 Jun 2019 02:20:42: 16000000 INFO @ Mon, 03 Jun 2019 02:20:48: 16000000 INFO @ Mon, 03 Jun 2019 02:20:48: 17000000 INFO @ Mon, 03 Jun 2019 02:20:48: 15000000 INFO @ Mon, 03 Jun 2019 02:20:55: 17000000 INFO @ Mon, 03 Jun 2019 02:20:55: 18000000 INFO @ Mon, 03 Jun 2019 02:20:56: 16000000 INFO @ Mon, 03 Jun 2019 02:21:01: 18000000 INFO @ Mon, 03 Jun 2019 02:21:02: 19000000 INFO @ Mon, 03 Jun 2019 02:21:03: 17000000 INFO @ Mon, 03 Jun 2019 02:21:08: 19000000 INFO @ Mon, 03 Jun 2019 02:21:08: 20000000 INFO @ Mon, 03 Jun 2019 02:21:10: 18000000 INFO @ Mon, 03 Jun 2019 02:21:14: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:21:14: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:21:14: #1 total tags in treatment: 20818631 INFO @ Mon, 03 Jun 2019 02:21:14: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:21:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:21:14: #1 tags after filtering in treatment: 20818631 INFO @ Mon, 03 Jun 2019 02:21:14: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:21:14: #1 finished! INFO @ Mon, 03 Jun 2019 02:21:14: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:21:14: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:21:15: 20000000 INFO @ Mon, 03 Jun 2019 02:21:16: #2 number of paired peaks: 7 WARNING @ Mon, 03 Jun 2019 02:21:16: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:21:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:21:18: 19000000 INFO @ Mon, 03 Jun 2019 02:21:21: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:21:21: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:21:21: #1 total tags in treatment: 20818631 INFO @ Mon, 03 Jun 2019 02:21:21: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:21:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:21:22: #1 tags after filtering in treatment: 20818631 INFO @ Mon, 03 Jun 2019 02:21:22: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:21:22: #1 finished! INFO @ Mon, 03 Jun 2019 02:21:22: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:21:22: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:21:23: #2 number of paired peaks: 7 WARNING @ Mon, 03 Jun 2019 02:21:23: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:21:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:21:25: 20000000 INFO @ Mon, 03 Jun 2019 02:21:31: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:21:31: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:21:31: #1 total tags in treatment: 20818631 INFO @ Mon, 03 Jun 2019 02:21:31: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:21:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:21:32: #1 tags after filtering in treatment: 20818631 INFO @ Mon, 03 Jun 2019 02:21:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:21:32: #1 finished! INFO @ Mon, 03 Jun 2019 02:21:32: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:21:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:21:34: #2 number of paired peaks: 7 WARNING @ Mon, 03 Jun 2019 02:21:34: Too few paired peaks (7) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:21:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111799/SRX111799.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。