Job ID = 9158288 sra ファイルのダウンロード中... Completed: 494623K bytes transferred in 7 seconds (530981K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 16750056 spots for /home/okishinya/chipatlas/results/dm3/SRX111794/SRR390247.sra Written 16750056 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:08 16750056 reads; of these: 16750056 (100.00%) were unpaired; of these: 246855 (1.47%) aligned 0 times 13879453 (82.86%) aligned exactly 1 time 2623748 (15.66%) aligned >1 times 98.53% overall alignment rate Time searching: 00:05:08 Overall time: 00:05:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3517805 / 16503201 = 0.2132 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:05:52: # Command line: callpeak -t SRX111794.bam -f BAM -g dm -n SRX111794.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX111794.10 # format = BAM # ChIP-seq file = ['SRX111794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:05:52: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:05:52: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:05:52: # Command line: callpeak -t SRX111794.bam -f BAM -g dm -n SRX111794.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX111794.20 # format = BAM # ChIP-seq file = ['SRX111794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:05:52: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:05:52: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:05:52: # Command line: callpeak -t SRX111794.bam -f BAM -g dm -n SRX111794.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX111794.05 # format = BAM # ChIP-seq file = ['SRX111794.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:05:52: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:05:52: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:05:59: 1000000 INFO @ Tue, 27 Jun 2017 16:05:59: 1000000 INFO @ Tue, 27 Jun 2017 16:05:59: 1000000 INFO @ Tue, 27 Jun 2017 16:06:05: 2000000 INFO @ Tue, 27 Jun 2017 16:06:05: 2000000 INFO @ Tue, 27 Jun 2017 16:06:05: 2000000 INFO @ Tue, 27 Jun 2017 16:06:12: 3000000 INFO @ Tue, 27 Jun 2017 16:06:12: 3000000 INFO @ Tue, 27 Jun 2017 16:06:12: 3000000 INFO @ Tue, 27 Jun 2017 16:06:18: 4000000 INFO @ Tue, 27 Jun 2017 16:06:18: 4000000 INFO @ Tue, 27 Jun 2017 16:06:18: 4000000 INFO @ Tue, 27 Jun 2017 16:06:25: 5000000 INFO @ Tue, 27 Jun 2017 16:06:25: 5000000 INFO @ Tue, 27 Jun 2017 16:06:25: 5000000 INFO @ Tue, 27 Jun 2017 16:06:31: 6000000 INFO @ Tue, 27 Jun 2017 16:06:31: 6000000 INFO @ Tue, 27 Jun 2017 16:06:32: 6000000 INFO @ Tue, 27 Jun 2017 16:06:38: 7000000 INFO @ Tue, 27 Jun 2017 16:06:38: 7000000 INFO @ Tue, 27 Jun 2017 16:06:39: 7000000 INFO @ Tue, 27 Jun 2017 16:06:44: 8000000 INFO @ Tue, 27 Jun 2017 16:06:45: 8000000 INFO @ Tue, 27 Jun 2017 16:06:46: 8000000 INFO @ Tue, 27 Jun 2017 16:06:51: 9000000 INFO @ Tue, 27 Jun 2017 16:06:51: 9000000 INFO @ Tue, 27 Jun 2017 16:06:52: 9000000 INFO @ Tue, 27 Jun 2017 16:06:57: 10000000 INFO @ Tue, 27 Jun 2017 16:06:58: 10000000 INFO @ Tue, 27 Jun 2017 16:06:59: 10000000 INFO @ Tue, 27 Jun 2017 16:07:04: 11000000 INFO @ Tue, 27 Jun 2017 16:07:05: 11000000 INFO @ Tue, 27 Jun 2017 16:07:06: 11000000 INFO @ Tue, 27 Jun 2017 16:07:11: 12000000 INFO @ Tue, 27 Jun 2017 16:07:11: 12000000 INFO @ Tue, 27 Jun 2017 16:07:13: 12000000 INFO @ Tue, 27 Jun 2017 16:07:17: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:07:17: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:07:17: #1 total tags in treatment: 12985396 INFO @ Tue, 27 Jun 2017 16:07:17: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:07:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:07:17: #1 tags after filtering in treatment: 12985396 INFO @ Tue, 27 Jun 2017 16:07:17: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:07:17: #1 finished! INFO @ Tue, 27 Jun 2017 16:07:17: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:07:17: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:07:18: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:07:18: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:07:18: #1 total tags in treatment: 12985396 INFO @ Tue, 27 Jun 2017 16:07:18: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:07:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:07:18: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:07:18: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:07:18: Process for pairing-model is terminated! cat: SRX111794.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111794.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111794.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111794.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:07:18: #1 tags after filtering in treatment: 12985396 INFO @ Tue, 27 Jun 2017 16:07:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:07:18: #1 finished! INFO @ Tue, 27 Jun 2017 16:07:18: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:07:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:07:19: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:07:19: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:07:19: Process for pairing-model is terminated! cat: SRX111794.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません INFO @ Tue, 27 Jun 2017 16:07:19: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:07:19: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:07:19: #1 total tags in treatment: 12985396 INFO @ Tue, 27 Jun 2017 16:07:19: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:07:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111794.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111794.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111794.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:07:19: #1 tags after filtering in treatment: 12985396 INFO @ Tue, 27 Jun 2017 16:07:19: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:07:19: #1 finished! INFO @ Tue, 27 Jun 2017 16:07:19: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:07:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:07:20: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:07:20: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:07:20: Process for pairing-model is terminated! cat: SRX111794.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX111794.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111794.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX111794.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。