Job ID = 1293704 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 18,098,421 reads read : 18,098,421 reads written : 18,098,421 spots read : 15,841,233 reads read : 15,841,233 reads written : 15,841,233 rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:51 33939654 reads; of these: 33939654 (100.00%) were unpaired; of these: 2048020 (6.03%) aligned 0 times 25173238 (74.17%) aligned exactly 1 time 6718396 (19.80%) aligned >1 times 93.97% overall alignment rate Time searching: 00:09:51 Overall time: 00:09:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10107856 / 31891634 = 0.3169 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Mon, 03 Jun 2019 02:08:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:08:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:08:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:08:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:08:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:08:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:08:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 03 Jun 2019 02:08:26: #1 read tag files... INFO @ Mon, 03 Jun 2019 02:08:26: #1 read treatment tags... INFO @ Mon, 03 Jun 2019 02:08:33: 1000000 INFO @ Mon, 03 Jun 2019 02:08:33: 1000000 INFO @ Mon, 03 Jun 2019 02:08:34: 1000000 INFO @ Mon, 03 Jun 2019 02:08:40: 2000000 INFO @ Mon, 03 Jun 2019 02:08:40: 2000000 INFO @ Mon, 03 Jun 2019 02:08:42: 2000000 INFO @ Mon, 03 Jun 2019 02:08:48: 3000000 INFO @ Mon, 03 Jun 2019 02:08:48: 3000000 INFO @ Mon, 03 Jun 2019 02:08:50: 3000000 INFO @ Mon, 03 Jun 2019 02:08:55: 4000000 INFO @ Mon, 03 Jun 2019 02:08:55: 4000000 INFO @ Mon, 03 Jun 2019 02:08:58: 4000000 INFO @ Mon, 03 Jun 2019 02:09:02: 5000000 INFO @ Mon, 03 Jun 2019 02:09:02: 5000000 INFO @ Mon, 03 Jun 2019 02:09:06: 5000000 INFO @ Mon, 03 Jun 2019 02:09:09: 6000000 INFO @ Mon, 03 Jun 2019 02:09:09: 6000000 INFO @ Mon, 03 Jun 2019 02:09:14: 6000000 INFO @ Mon, 03 Jun 2019 02:09:16: 7000000 INFO @ Mon, 03 Jun 2019 02:09:16: 7000000 INFO @ Mon, 03 Jun 2019 02:09:21: 7000000 INFO @ Mon, 03 Jun 2019 02:09:22: 8000000 INFO @ Mon, 03 Jun 2019 02:09:22: 8000000 INFO @ Mon, 03 Jun 2019 02:09:29: 8000000 INFO @ Mon, 03 Jun 2019 02:09:29: 9000000 INFO @ Mon, 03 Jun 2019 02:09:29: 9000000 INFO @ Mon, 03 Jun 2019 02:09:36: 10000000 INFO @ Mon, 03 Jun 2019 02:09:36: 10000000 INFO @ Mon, 03 Jun 2019 02:09:37: 9000000 INFO @ Mon, 03 Jun 2019 02:09:43: 11000000 INFO @ Mon, 03 Jun 2019 02:09:43: 11000000 INFO @ Mon, 03 Jun 2019 02:09:44: 10000000 INFO @ Mon, 03 Jun 2019 02:09:49: 12000000 INFO @ Mon, 03 Jun 2019 02:09:50: 12000000 INFO @ Mon, 03 Jun 2019 02:09:52: 11000000 INFO @ Mon, 03 Jun 2019 02:09:56: 13000000 INFO @ Mon, 03 Jun 2019 02:09:56: 13000000 INFO @ Mon, 03 Jun 2019 02:10:00: 12000000 INFO @ Mon, 03 Jun 2019 02:10:03: 14000000 INFO @ Mon, 03 Jun 2019 02:10:03: 14000000 INFO @ Mon, 03 Jun 2019 02:10:07: 13000000 INFO @ Mon, 03 Jun 2019 02:10:10: 15000000 INFO @ Mon, 03 Jun 2019 02:10:10: 15000000 INFO @ Mon, 03 Jun 2019 02:10:15: 14000000 INFO @ Mon, 03 Jun 2019 02:10:16: 16000000 INFO @ Mon, 03 Jun 2019 02:10:17: 16000000 INFO @ Mon, 03 Jun 2019 02:10:22: 15000000 INFO @ Mon, 03 Jun 2019 02:10:23: 17000000 INFO @ Mon, 03 Jun 2019 02:10:24: 17000000 INFO @ Mon, 03 Jun 2019 02:10:29: 16000000 INFO @ Mon, 03 Jun 2019 02:10:30: 18000000 INFO @ Mon, 03 Jun 2019 02:10:31: 18000000 INFO @ Mon, 03 Jun 2019 02:10:37: 17000000 INFO @ Mon, 03 Jun 2019 02:10:38: 19000000 INFO @ Mon, 03 Jun 2019 02:10:38: 19000000 INFO @ Mon, 03 Jun 2019 02:10:44: 20000000 INFO @ Mon, 03 Jun 2019 02:10:45: 20000000 INFO @ Mon, 03 Jun 2019 02:10:45: 18000000 INFO @ Mon, 03 Jun 2019 02:10:51: 21000000 INFO @ Mon, 03 Jun 2019 02:10:51: 21000000 INFO @ Mon, 03 Jun 2019 02:10:53: 19000000 INFO @ Mon, 03 Jun 2019 02:10:57: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:10:57: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:10:57: #1 total tags in treatment: 21783778 INFO @ Mon, 03 Jun 2019 02:10:57: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:10:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:10:57: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:10:57: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:10:57: #1 total tags in treatment: 21783778 INFO @ Mon, 03 Jun 2019 02:10:57: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:10:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:10:57: #1 tags after filtering in treatment: 21783778 INFO @ Mon, 03 Jun 2019 02:10:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:10:57: #1 finished! INFO @ Mon, 03 Jun 2019 02:10:57: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:10:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:10:57: #1 tags after filtering in treatment: 21783778 INFO @ Mon, 03 Jun 2019 02:10:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:10:57: #1 finished! INFO @ Mon, 03 Jun 2019 02:10:57: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:10:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:10:59: #2 number of paired peaks: 24 WARNING @ Mon, 03 Jun 2019 02:10:59: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:10:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:10:59: #2 number of paired peaks: 24 WARNING @ Mon, 03 Jun 2019 02:10:59: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:10:59: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 03 Jun 2019 02:11:01: 20000000 INFO @ Mon, 03 Jun 2019 02:11:09: 21000000 INFO @ Mon, 03 Jun 2019 02:11:15: #1 tag size is determined as 44 bps INFO @ Mon, 03 Jun 2019 02:11:15: #1 tag size = 44 INFO @ Mon, 03 Jun 2019 02:11:15: #1 total tags in treatment: 21783778 INFO @ Mon, 03 Jun 2019 02:11:15: #1 user defined the maximum tags... INFO @ Mon, 03 Jun 2019 02:11:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 03 Jun 2019 02:11:15: #1 tags after filtering in treatment: 21783778 INFO @ Mon, 03 Jun 2019 02:11:15: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 03 Jun 2019 02:11:15: #1 finished! INFO @ Mon, 03 Jun 2019 02:11:15: #2 Build Peak Model... INFO @ Mon, 03 Jun 2019 02:11:15: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 03 Jun 2019 02:11:17: #2 number of paired peaks: 24 WARNING @ Mon, 03 Jun 2019 02:11:17: Too few paired peaks (24) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 03 Jun 2019 02:11:17: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX111784/SRX111784.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。