Job ID = 10480648 sra ファイルのダウンロード中... Completed: 590445K bytes transferred in 10 seconds (469024K bits/sec), in 1 file. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 24335275 spots for /home/okishinya/chipatlas/results/dm3/SRX1115693/SRR2124122.sra Written 24335275 spots total rm: cannot remove `[DSE]RX*': そのようなファイルやディレクトリはありません rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:07:43 24335275 reads; of these: 24335275 (100.00%) were unpaired; of these: 863319 (3.55%) aligned 0 times 17894942 (73.53%) aligned exactly 1 time 5577014 (22.92%) aligned >1 times 96.45% overall alignment rate Time searching: 00:07:44 Overall time: 00:07:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 2851013 / 23471956 = 0.1215 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Fri, 16 Mar 2018 07:38:11: # Command line: callpeak -t SRX1115693.bam -f BAM -g dm -n SRX1115693.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX1115693.05 # format = BAM # ChIP-seq file = ['SRX1115693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Mar 2018 07:38:11: #1 read tag files... INFO @ Fri, 16 Mar 2018 07:38:11: #1 read treatment tags... INFO @ Fri, 16 Mar 2018 07:38:11: # Command line: callpeak -t SRX1115693.bam -f BAM -g dm -n SRX1115693.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX1115693.20 # format = BAM # ChIP-seq file = ['SRX1115693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Mar 2018 07:38:11: #1 read tag files... INFO @ Fri, 16 Mar 2018 07:38:11: #1 read treatment tags... INFO @ Fri, 16 Mar 2018 07:38:11: # Command line: callpeak -t SRX1115693.bam -f BAM -g dm -n SRX1115693.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX1115693.10 # format = BAM # ChIP-seq file = ['SRX1115693.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 16 Mar 2018 07:38:11: #1 read tag files... INFO @ Fri, 16 Mar 2018 07:38:11: #1 read treatment tags... INFO @ Fri, 16 Mar 2018 07:38:18: 1000000 INFO @ Fri, 16 Mar 2018 07:38:18: 1000000 INFO @ Fri, 16 Mar 2018 07:38:18: 1000000 INFO @ Fri, 16 Mar 2018 07:38:24: 2000000 INFO @ Fri, 16 Mar 2018 07:38:25: 2000000 INFO @ Fri, 16 Mar 2018 07:38:26: 2000000 INFO @ Fri, 16 Mar 2018 07:38:31: 3000000 INFO @ Fri, 16 Mar 2018 07:38:32: 3000000 INFO @ Fri, 16 Mar 2018 07:38:33: 3000000 INFO @ Fri, 16 Mar 2018 07:38:38: 4000000 INFO @ Fri, 16 Mar 2018 07:38:39: 4000000 INFO @ Fri, 16 Mar 2018 07:38:40: 4000000 INFO @ Fri, 16 Mar 2018 07:38:45: 5000000 INFO @ Fri, 16 Mar 2018 07:38:46: 5000000 INFO @ Fri, 16 Mar 2018 07:38:47: 5000000 INFO @ Fri, 16 Mar 2018 07:38:52: 6000000 INFO @ Fri, 16 Mar 2018 07:38:53: 6000000 INFO @ Fri, 16 Mar 2018 07:38:55: 6000000 INFO @ Fri, 16 Mar 2018 07:38:59: 7000000 INFO @ Fri, 16 Mar 2018 07:39:00: 7000000 INFO @ Fri, 16 Mar 2018 07:39:02: 7000000 INFO @ Fri, 16 Mar 2018 07:39:06: 8000000 INFO @ Fri, 16 Mar 2018 07:39:08: 8000000 INFO @ Fri, 16 Mar 2018 07:39:09: 8000000 INFO @ Fri, 16 Mar 2018 07:39:13: 9000000 INFO @ Fri, 16 Mar 2018 07:39:15: 9000000 INFO @ Fri, 16 Mar 2018 07:39:16: 9000000 INFO @ Fri, 16 Mar 2018 07:39:20: 10000000 INFO @ Fri, 16 Mar 2018 07:39:22: 10000000 INFO @ Fri, 16 Mar 2018 07:39:24: 10000000 INFO @ Fri, 16 Mar 2018 07:39:26: 11000000 INFO @ Fri, 16 Mar 2018 07:39:28: 11000000 INFO @ Fri, 16 Mar 2018 07:39:32: 11000000 INFO @ Fri, 16 Mar 2018 07:39:32: 12000000 INFO @ Fri, 16 Mar 2018 07:39:35: 12000000 INFO @ Fri, 16 Mar 2018 07:39:39: 13000000 INFO @ Fri, 16 Mar 2018 07:39:39: 12000000 INFO @ Fri, 16 Mar 2018 07:39:41: 13000000 INFO @ Fri, 16 Mar 2018 07:39:45: 14000000 INFO @ Fri, 16 Mar 2018 07:39:47: 13000000 INFO @ Fri, 16 Mar 2018 07:39:48: 14000000 INFO @ Fri, 16 Mar 2018 07:39:52: 15000000 INFO @ Fri, 16 Mar 2018 07:39:54: 15000000 INFO @ Fri, 16 Mar 2018 07:39:54: 14000000 INFO @ Fri, 16 Mar 2018 07:40:00: 16000000 INFO @ Fri, 16 Mar 2018 07:40:01: 16000000 INFO @ Fri, 16 Mar 2018 07:40:02: 15000000 INFO @ Fri, 16 Mar 2018 07:40:07: 17000000 INFO @ Fri, 16 Mar 2018 07:40:09: 17000000 INFO @ Fri, 16 Mar 2018 07:40:10: 16000000 INFO @ Fri, 16 Mar 2018 07:40:14: 18000000 INFO @ Fri, 16 Mar 2018 07:40:18: 18000000 INFO @ Fri, 16 Mar 2018 07:40:18: 17000000 INFO @ Fri, 16 Mar 2018 07:40:20: 19000000 INFO @ Fri, 16 Mar 2018 07:40:26: 18000000 INFO @ Fri, 16 Mar 2018 07:40:26: 19000000 INFO @ Fri, 16 Mar 2018 07:40:27: 20000000 INFO @ Fri, 16 Mar 2018 07:40:31: #1 tag size is determined as 51 bps INFO @ Fri, 16 Mar 2018 07:40:31: #1 tag size = 51 INFO @ Fri, 16 Mar 2018 07:40:31: #1 total tags in treatment: 20620943 INFO @ Fri, 16 Mar 2018 07:40:31: #1 user defined the maximum tags... INFO @ Fri, 16 Mar 2018 07:40:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Mar 2018 07:40:31: #1 tags after filtering in treatment: 20620943 INFO @ Fri, 16 Mar 2018 07:40:31: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Mar 2018 07:40:31: #1 finished! INFO @ Fri, 16 Mar 2018 07:40:31: #2 Build Peak Model... INFO @ Fri, 16 Mar 2018 07:40:31: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Mar 2018 07:40:33: #2 number of paired peaks: 17 WARNING @ Fri, 16 Mar 2018 07:40:33: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Mar 2018 07:40:33: Process for pairing-model is terminated! cat: SRX1115693.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1115693.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1115693.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1115693.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 16 Mar 2018 07:40:34: 19000000 INFO @ Fri, 16 Mar 2018 07:40:35: 20000000 INFO @ Fri, 16 Mar 2018 07:40:39: #1 tag size is determined as 51 bps INFO @ Fri, 16 Mar 2018 07:40:39: #1 tag size = 51 INFO @ Fri, 16 Mar 2018 07:40:39: #1 total tags in treatment: 20620943 INFO @ Fri, 16 Mar 2018 07:40:39: #1 user defined the maximum tags... INFO @ Fri, 16 Mar 2018 07:40:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Mar 2018 07:40:39: #1 tags after filtering in treatment: 20620943 INFO @ Fri, 16 Mar 2018 07:40:39: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Mar 2018 07:40:39: #1 finished! INFO @ Fri, 16 Mar 2018 07:40:39: #2 Build Peak Model... INFO @ Fri, 16 Mar 2018 07:40:39: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Mar 2018 07:40:40: 20000000 INFO @ Fri, 16 Mar 2018 07:40:40: #2 number of paired peaks: 17 WARNING @ Fri, 16 Mar 2018 07:40:40: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Mar 2018 07:40:40: Process for pairing-model is terminated! cat: SRX1115693.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1115693.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1115693.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1115693.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Fri, 16 Mar 2018 07:40:44: #1 tag size is determined as 51 bps INFO @ Fri, 16 Mar 2018 07:40:44: #1 tag size = 51 INFO @ Fri, 16 Mar 2018 07:40:44: #1 total tags in treatment: 20620943 INFO @ Fri, 16 Mar 2018 07:40:44: #1 user defined the maximum tags... INFO @ Fri, 16 Mar 2018 07:40:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 16 Mar 2018 07:40:45: #1 tags after filtering in treatment: 20620943 INFO @ Fri, 16 Mar 2018 07:40:45: #1 Redundant rate of treatment: 0.00 INFO @ Fri, 16 Mar 2018 07:40:45: #1 finished! INFO @ Fri, 16 Mar 2018 07:40:45: #2 Build Peak Model... INFO @ Fri, 16 Mar 2018 07:40:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 16 Mar 2018 07:40:46: #2 number of paired peaks: 17 WARNING @ Fri, 16 Mar 2018 07:40:46: Too few paired peaks (17) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Fri, 16 Mar 2018 07:40:46: Process for pairing-model is terminated! cat: SRX1115693.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX1115693.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1115693.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX1115693.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。