Job ID = 6527551 SRX = SRX110788 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:58:40 prefetch.2.10.7: 1) Downloading 'SRR388370'... 2020-06-29T12:58:40 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:59:22 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:59:22 prefetch.2.10.7: 'SRR388370' is valid 2020-06-29T12:59:22 prefetch.2.10.7: 1) 'SRR388370' was downloaded successfully Read 16261620 spots for SRR388370/SRR388370.sra Written 16261620 spots for SRR388370/SRR388370.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:50 16261620 reads; of these: 16261620 (100.00%) were unpaired; of these: 369091 (2.27%) aligned 0 times 7731366 (47.54%) aligned exactly 1 time 8161163 (50.19%) aligned >1 times 97.73% overall alignment rate Time searching: 00:05:51 Overall time: 00:05:51 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 1006947 / 15892529 = 0.0634 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:11:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:11:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:11:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:11:41: 1000000 INFO @ Mon, 29 Jun 2020 22:11:45: 2000000 INFO @ Mon, 29 Jun 2020 22:11:49: 3000000 INFO @ Mon, 29 Jun 2020 22:11:53: 4000000 INFO @ Mon, 29 Jun 2020 22:11:57: 5000000 INFO @ Mon, 29 Jun 2020 22:12:01: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:12:05: 7000000 INFO @ Mon, 29 Jun 2020 22:12:06: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:12:06: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:12:06: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:12:10: 8000000 INFO @ Mon, 29 Jun 2020 22:12:11: 1000000 INFO @ Mon, 29 Jun 2020 22:12:14: 9000000 INFO @ Mon, 29 Jun 2020 22:12:16: 2000000 INFO @ Mon, 29 Jun 2020 22:12:18: 10000000 INFO @ Mon, 29 Jun 2020 22:12:20: 3000000 INFO @ Mon, 29 Jun 2020 22:12:22: 11000000 INFO @ Mon, 29 Jun 2020 22:12:24: 4000000 INFO @ Mon, 29 Jun 2020 22:12:26: 12000000 INFO @ Mon, 29 Jun 2020 22:12:29: 5000000 INFO @ Mon, 29 Jun 2020 22:12:30: 13000000 INFO @ Mon, 29 Jun 2020 22:12:33: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:12:35: 14000000 INFO @ Mon, 29 Jun 2020 22:12:36: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:12:36: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:12:36: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:12:38: 7000000 INFO @ Mon, 29 Jun 2020 22:12:39: #1 tag size is determined as 18 bps INFO @ Mon, 29 Jun 2020 22:12:39: #1 tag size = 18 INFO @ Mon, 29 Jun 2020 22:12:39: #1 total tags in treatment: 14885582 INFO @ Mon, 29 Jun 2020 22:12:39: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:12:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:12:39: #1 tags after filtering in treatment: 14885582 INFO @ Mon, 29 Jun 2020 22:12:39: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:12:39: #1 finished! INFO @ Mon, 29 Jun 2020 22:12:39: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:12:39: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:12:40: #2 number of paired peaks: 95 WARNING @ Mon, 29 Jun 2020 22:12:40: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:12:40: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:12:41: 1000000 INFO @ Mon, 29 Jun 2020 22:12:43: 8000000 INFO @ Mon, 29 Jun 2020 22:12:45: 2000000 INFO @ Mon, 29 Jun 2020 22:12:47: 9000000 INFO @ Mon, 29 Jun 2020 22:12:50: 3000000 INFO @ Mon, 29 Jun 2020 22:12:51: 10000000 INFO @ Mon, 29 Jun 2020 22:12:54: 4000000 INFO @ Mon, 29 Jun 2020 22:12:56: 11000000 INFO @ Mon, 29 Jun 2020 22:12:58: 5000000 INFO @ Mon, 29 Jun 2020 22:13:00: 12000000 INFO @ Mon, 29 Jun 2020 22:13:03: 6000000 INFO @ Mon, 29 Jun 2020 22:13:05: 13000000 INFO @ Mon, 29 Jun 2020 22:13:07: 7000000 INFO @ Mon, 29 Jun 2020 22:13:09: 14000000 INFO @ Mon, 29 Jun 2020 22:13:11: 8000000 INFO @ Mon, 29 Jun 2020 22:13:13: #1 tag size is determined as 18 bps INFO @ Mon, 29 Jun 2020 22:13:13: #1 tag size = 18 INFO @ Mon, 29 Jun 2020 22:13:13: #1 total tags in treatment: 14885582 INFO @ Mon, 29 Jun 2020 22:13:13: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:13:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:13:13: #1 tags after filtering in treatment: 14885582 INFO @ Mon, 29 Jun 2020 22:13:13: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:13:13: #1 finished! INFO @ Mon, 29 Jun 2020 22:13:13: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:13:13: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:13:14: #2 number of paired peaks: 95 WARNING @ Mon, 29 Jun 2020 22:13:14: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:13:14: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:13:16: 9000000 INFO @ Mon, 29 Jun 2020 22:13:20: 10000000 INFO @ Mon, 29 Jun 2020 22:13:24: 11000000 INFO @ Mon, 29 Jun 2020 22:13:28: 12000000 INFO @ Mon, 29 Jun 2020 22:13:32: 13000000 INFO @ Mon, 29 Jun 2020 22:13:37: 14000000 INFO @ Mon, 29 Jun 2020 22:13:41: #1 tag size is determined as 18 bps INFO @ Mon, 29 Jun 2020 22:13:41: #1 tag size = 18 INFO @ Mon, 29 Jun 2020 22:13:41: #1 total tags in treatment: 14885582 INFO @ Mon, 29 Jun 2020 22:13:41: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:13:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:13:41: #1 tags after filtering in treatment: 14885582 INFO @ Mon, 29 Jun 2020 22:13:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:13:41: #1 finished! INFO @ Mon, 29 Jun 2020 22:13:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:13:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:13:42: #2 number of paired peaks: 95 WARNING @ Mon, 29 Jun 2020 22:13:42: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:13:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX110788/SRX110788.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。