Job ID = 14168452 SRX = SRX11078131 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2021-12-10T09:28:07 prefetch.2.10.7: 1) Downloading 'SRR14743559'... 2021-12-10T09:28:07 prefetch.2.10.7: Downloading via HTTPS... 2021-12-10T09:28:21 prefetch.2.10.7: HTTPS download succeed 2021-12-10T09:28:21 prefetch.2.10.7: 'SRR14743559' is valid 2021-12-10T09:28:21 prefetch.2.10.7: 1) 'SRR14743559' was downloaded successfully 2021-12-10T09:28:21 prefetch.2.10.7: 'SRR14743559' has 0 unresolved dependencies Read 6683907 spots for SRR14743559/SRR14743559.sra Written 6683907 spots for SRR14743559/SRR14743559.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169582 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:10 6683907 reads; of these: 6683907 (100.00%) were paired; of these: 6304786 (94.33%) aligned concordantly 0 times 280429 (4.20%) aligned concordantly exactly 1 time 98692 (1.48%) aligned concordantly >1 times ---- 6304786 pairs aligned concordantly 0 times; of these: 872 (0.01%) aligned discordantly 1 time ---- 6303914 pairs aligned 0 times concordantly or discordantly; of these: 12607828 mates make up the pairs; of these: 12555904 (99.59%) aligned 0 times 18493 (0.15%) aligned exactly 1 time 33431 (0.27%) aligned >1 times 6.07% overall alignment rate Time searching: 00:01:10 Overall time: 00:01:10 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 9035 / 379737 = 0.0238 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:30:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:30:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:30:45: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:30:48: #1 tag size is determined as 40 bps INFO @ Fri, 10 Dec 2021 18:30:48: #1 tag size = 40 INFO @ Fri, 10 Dec 2021 18:30:48: #1 total tags in treatment: 370100 INFO @ Fri, 10 Dec 2021 18:30:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:30:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:30:48: #1 tags after filtering in treatment: 365828 INFO @ Fri, 10 Dec 2021 18:30:48: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 10 Dec 2021 18:30:48: #1 finished! INFO @ Fri, 10 Dec 2021 18:30:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:30:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:30:48: #2 number of paired peaks: 668 WARNING @ Fri, 10 Dec 2021 18:30:48: Fewer paired peaks (668) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 668 pairs to build model! INFO @ Fri, 10 Dec 2021 18:30:48: start model_add_line... INFO @ Fri, 10 Dec 2021 18:30:48: start X-correlation... INFO @ Fri, 10 Dec 2021 18:30:48: end of X-cor INFO @ Fri, 10 Dec 2021 18:30:48: #2 finished! INFO @ Fri, 10 Dec 2021 18:30:48: #2 predicted fragment length is 96 bps INFO @ Fri, 10 Dec 2021 18:30:48: #2 alternative fragment length(s) may be 96 bps INFO @ Fri, 10 Dec 2021 18:30:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.05_model.r INFO @ Fri, 10 Dec 2021 18:30:48: #3 Call peaks... INFO @ Fri, 10 Dec 2021 18:30:48: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 18:30:49: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 18:30:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.05_peaks.xls INFO @ Fri, 10 Dec 2021 18:30:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 18:30:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.05_summits.bed INFO @ Fri, 10 Dec 2021 18:30:49: Done! pass1 - making usageList (5 chroms): 1 millis pass2 - checking and writing primary data (62 records, 4 fields): 1 millis CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:31:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:31:15: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:31:15: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 18:31:18: #1 tag size is determined as 40 bps INFO @ Fri, 10 Dec 2021 18:31:18: #1 tag size = 40 INFO @ Fri, 10 Dec 2021 18:31:18: #1 total tags in treatment: 370100 INFO @ Fri, 10 Dec 2021 18:31:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:31:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:31:18: #1 tags after filtering in treatment: 365828 INFO @ Fri, 10 Dec 2021 18:31:18: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 10 Dec 2021 18:31:18: #1 finished! INFO @ Fri, 10 Dec 2021 18:31:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:31:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:31:18: #2 number of paired peaks: 668 WARNING @ Fri, 10 Dec 2021 18:31:18: Fewer paired peaks (668) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 668 pairs to build model! INFO @ Fri, 10 Dec 2021 18:31:18: start model_add_line... INFO @ Fri, 10 Dec 2021 18:31:18: start X-correlation... INFO @ Fri, 10 Dec 2021 18:31:18: end of X-cor INFO @ Fri, 10 Dec 2021 18:31:18: #2 finished! INFO @ Fri, 10 Dec 2021 18:31:18: #2 predicted fragment length is 96 bps INFO @ Fri, 10 Dec 2021 18:31:18: #2 alternative fragment length(s) may be 96 bps INFO @ Fri, 10 Dec 2021 18:31:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.10_model.r INFO @ Fri, 10 Dec 2021 18:31:18: #3 Call peaks... INFO @ Fri, 10 Dec 2021 18:31:18: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 18:31:19: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 18:31:19: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.10_peaks.xls INFO @ Fri, 10 Dec 2021 18:31:19: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 18:31:19: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.10_summits.bed INFO @ Fri, 10 Dec 2021 18:31:19: Done! pass1 - making usageList (4 chroms): 1 millis pass2 - checking and writing primary data (41 records, 4 fields): 1 millis CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 18:31:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 18:31:45: #1 read tag files... INFO @ Fri, 10 Dec 2021 18:31:45: #1 read treatment tags... BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 18:31:48: #1 tag size is determined as 40 bps INFO @ Fri, 10 Dec 2021 18:31:48: #1 tag size = 40 INFO @ Fri, 10 Dec 2021 18:31:48: #1 total tags in treatment: 370100 INFO @ Fri, 10 Dec 2021 18:31:48: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 18:31:48: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 18:31:48: #1 tags after filtering in treatment: 365828 INFO @ Fri, 10 Dec 2021 18:31:48: #1 Redundant rate of treatment: 0.01 INFO @ Fri, 10 Dec 2021 18:31:48: #1 finished! INFO @ Fri, 10 Dec 2021 18:31:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 18:31:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 18:31:48: #2 number of paired peaks: 668 WARNING @ Fri, 10 Dec 2021 18:31:48: Fewer paired peaks (668) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 668 pairs to build model! INFO @ Fri, 10 Dec 2021 18:31:48: start model_add_line... INFO @ Fri, 10 Dec 2021 18:31:48: start X-correlation... INFO @ Fri, 10 Dec 2021 18:31:48: end of X-cor INFO @ Fri, 10 Dec 2021 18:31:48: #2 finished! INFO @ Fri, 10 Dec 2021 18:31:48: #2 predicted fragment length is 96 bps INFO @ Fri, 10 Dec 2021 18:31:48: #2 alternative fragment length(s) may be 96 bps INFO @ Fri, 10 Dec 2021 18:31:48: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.20_model.r INFO @ Fri, 10 Dec 2021 18:31:48: #3 Call peaks... INFO @ Fri, 10 Dec 2021 18:31:48: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 18:31:49: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 18:31:49: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.20_peaks.xls INFO @ Fri, 10 Dec 2021 18:31:49: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 18:31:49: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX11078131/SRX11078131.20_summits.bed INFO @ Fri, 10 Dec 2021 18:31:49: Done! pass1 - making usageList (3 chroms): 0 millis pass2 - checking and writing primary data (31 records, 4 fields): 1 millis CompletedMACS2peakCalling