Job ID = 9158201 sra ファイルのダウンロード中... Completed: 1301366K bytes transferred in 13 seconds (777101K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 19141145 spots for /home/okishinya/chipatlas/results/dm3/SRX110510/SRR387831.sra Written 19141145 spots total Written 23287832 spots for /home/okishinya/chipatlas/results/dm3/SRX110510/SRR387832.sra Written 23287832 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:36 42428977 reads; of these: 42428977 (100.00%) were unpaired; of these: 5146180 (12.13%) aligned 0 times 32911845 (77.57%) aligned exactly 1 time 4370952 (10.30%) aligned >1 times 87.87% overall alignment rate Time searching: 00:10:37 Overall time: 00:10:37 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 10711506 / 37282797 = 0.2873 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:57:41: # Command line: callpeak -t SRX110510.bam -f BAM -g dm -n SRX110510.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX110510.05 # format = BAM # ChIP-seq file = ['SRX110510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:57:41: # Command line: callpeak -t SRX110510.bam -f BAM -g dm -n SRX110510.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX110510.20 # format = BAM # ChIP-seq file = ['SRX110510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:57:41: # Command line: callpeak -t SRX110510.bam -f BAM -g dm -n SRX110510.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX110510.10 # format = BAM # ChIP-seq file = ['SRX110510.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:57:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:57:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:57:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:57:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:57:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:57:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:57:49: 1000000 INFO @ Tue, 27 Jun 2017 15:57:49: 1000000 INFO @ Tue, 27 Jun 2017 15:57:49: 1000000 INFO @ Tue, 27 Jun 2017 15:57:57: 2000000 INFO @ Tue, 27 Jun 2017 15:57:57: 2000000 INFO @ Tue, 27 Jun 2017 15:57:57: 2000000 INFO @ Tue, 27 Jun 2017 15:58:04: 3000000 INFO @ Tue, 27 Jun 2017 15:58:04: 3000000 INFO @ Tue, 27 Jun 2017 15:58:04: 3000000 INFO @ Tue, 27 Jun 2017 15:58:12: 4000000 INFO @ Tue, 27 Jun 2017 15:58:12: 4000000 INFO @ Tue, 27 Jun 2017 15:58:12: 4000000 INFO @ Tue, 27 Jun 2017 15:58:20: 5000000 INFO @ Tue, 27 Jun 2017 15:58:20: 5000000 INFO @ Tue, 27 Jun 2017 15:58:20: 5000000 INFO @ Tue, 27 Jun 2017 15:58:27: 6000000 INFO @ Tue, 27 Jun 2017 15:58:27: 6000000 INFO @ Tue, 27 Jun 2017 15:58:27: 6000000 INFO @ Tue, 27 Jun 2017 15:58:35: 7000000 INFO @ Tue, 27 Jun 2017 15:58:35: 7000000 INFO @ Tue, 27 Jun 2017 15:58:35: 7000000 INFO @ Tue, 27 Jun 2017 15:58:43: 8000000 INFO @ Tue, 27 Jun 2017 15:58:43: 8000000 INFO @ Tue, 27 Jun 2017 15:58:43: 8000000 INFO @ Tue, 27 Jun 2017 15:58:51: 9000000 INFO @ Tue, 27 Jun 2017 15:58:51: 9000000 INFO @ Tue, 27 Jun 2017 15:58:51: 9000000 INFO @ Tue, 27 Jun 2017 15:58:59: 10000000 INFO @ Tue, 27 Jun 2017 15:58:59: 10000000 INFO @ Tue, 27 Jun 2017 15:58:59: 10000000 INFO @ Tue, 27 Jun 2017 15:59:06: 11000000 INFO @ Tue, 27 Jun 2017 15:59:06: 11000000 INFO @ Tue, 27 Jun 2017 15:59:06: 11000000 INFO @ Tue, 27 Jun 2017 15:59:13: 12000000 INFO @ Tue, 27 Jun 2017 15:59:14: 12000000 INFO @ Tue, 27 Jun 2017 15:59:14: 12000000 INFO @ Tue, 27 Jun 2017 15:59:20: 13000000 INFO @ Tue, 27 Jun 2017 15:59:21: 13000000 INFO @ Tue, 27 Jun 2017 15:59:21: 13000000 INFO @ Tue, 27 Jun 2017 15:59:27: 14000000 INFO @ Tue, 27 Jun 2017 15:59:29: 14000000 INFO @ Tue, 27 Jun 2017 15:59:29: 14000000 INFO @ Tue, 27 Jun 2017 15:59:36: 15000000 INFO @ Tue, 27 Jun 2017 15:59:36: 15000000 INFO @ Tue, 27 Jun 2017 15:59:37: 15000000 INFO @ Tue, 27 Jun 2017 15:59:43: 16000000 INFO @ Tue, 27 Jun 2017 15:59:44: 16000000 INFO @ Tue, 27 Jun 2017 15:59:45: 16000000 INFO @ Tue, 27 Jun 2017 15:59:50: 17000000 INFO @ Tue, 27 Jun 2017 15:59:53: 17000000 INFO @ Tue, 27 Jun 2017 15:59:54: 17000000 INFO @ Tue, 27 Jun 2017 15:59:57: 18000000 INFO @ Tue, 27 Jun 2017 16:00:02: 18000000 INFO @ Tue, 27 Jun 2017 16:00:03: 18000000 INFO @ Tue, 27 Jun 2017 16:00:04: 19000000 INFO @ Tue, 27 Jun 2017 16:00:10: 19000000 INFO @ Tue, 27 Jun 2017 16:00:11: 20000000 INFO @ Tue, 27 Jun 2017 16:00:12: 19000000 INFO @ Tue, 27 Jun 2017 16:00:18: 21000000 INFO @ Tue, 27 Jun 2017 16:00:19: 20000000 INFO @ Tue, 27 Jun 2017 16:00:20: 20000000 INFO @ Tue, 27 Jun 2017 16:00:25: 22000000 INFO @ Tue, 27 Jun 2017 16:00:28: 21000000 INFO @ Tue, 27 Jun 2017 16:00:29: 21000000 INFO @ Tue, 27 Jun 2017 16:00:32: 23000000 INFO @ Tue, 27 Jun 2017 16:00:36: 22000000 INFO @ Tue, 27 Jun 2017 16:00:38: 22000000 INFO @ Tue, 27 Jun 2017 16:00:39: 24000000 INFO @ Tue, 27 Jun 2017 16:00:45: 23000000 INFO @ Tue, 27 Jun 2017 16:00:46: 25000000 INFO @ Tue, 27 Jun 2017 16:00:46: 23000000 INFO @ Tue, 27 Jun 2017 16:00:53: 26000000 INFO @ Tue, 27 Jun 2017 16:00:54: 24000000 INFO @ Tue, 27 Jun 2017 16:00:56: 24000000 INFO @ Tue, 27 Jun 2017 16:00:58: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:00:58: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:00:58: #1 total tags in treatment: 26571291 INFO @ Tue, 27 Jun 2017 16:00:58: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:00:58: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:00:58: #1 tags after filtering in treatment: 26571291 INFO @ Tue, 27 Jun 2017 16:00:58: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:00:58: #1 finished! INFO @ Tue, 27 Jun 2017 16:00:58: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:00:58: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:01:00: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:01:00: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:01:00: Process for pairing-model is terminated! cat: SRX110510.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX110510.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110510.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110510.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:01:04: 25000000 INFO @ Tue, 27 Jun 2017 16:01:06: 25000000 INFO @ Tue, 27 Jun 2017 16:01:15: 26000000 INFO @ Tue, 27 Jun 2017 16:01:16: 26000000 INFO @ Tue, 27 Jun 2017 16:01:20: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:01:20: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:01:20: #1 total tags in treatment: 26571291 INFO @ Tue, 27 Jun 2017 16:01:20: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:01:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:01:21: #1 tags after filtering in treatment: 26571291 INFO @ Tue, 27 Jun 2017 16:01:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:01:21: #1 finished! INFO @ Tue, 27 Jun 2017 16:01:21: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:01:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:01:22: #1 tag size is determined as 44 bps INFO @ Tue, 27 Jun 2017 16:01:22: #1 tag size = 44 INFO @ Tue, 27 Jun 2017 16:01:22: #1 total tags in treatment: 26571291 INFO @ Tue, 27 Jun 2017 16:01:22: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:01:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:01:23: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:01:23: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:01:23: Process for pairing-model is terminated! cat: SRX110510.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) INFO @ Tue, 27 Jun 2017 16:01:23: #1 tags after filtering in treatment: 26571291 INFO @ Tue, 27 Jun 2017 16:01:23: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:01:23: #1 finished! INFO @ Tue, 27 Jun 2017 16:01:23: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:01:23: #2 looking for paired plus/minus strand peaks... rm: cannot remove `SRX110510.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110510.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110510.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:01:24: #2 number of paired peaks: 1 WARNING @ Tue, 27 Jun 2017 16:01:24: Too few paired peaks (1) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:01:24: Process for pairing-model is terminated! cat: SRX110510.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX110510.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110510.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX110510.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。