Job ID = 16440073 SRX = SRX10855785 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 31271998 spots for SRR14509972/SRR14509972.sra Written 31271998 spots for SRR14509972/SRR14509972.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440971 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:09:54 31271998 reads; of these: 31271998 (100.00%) were paired; of these: 2368285 (7.57%) aligned concordantly 0 times 21653496 (69.24%) aligned concordantly exactly 1 time 7250217 (23.18%) aligned concordantly >1 times ---- 2368285 pairs aligned concordantly 0 times; of these: 438376 (18.51%) aligned discordantly 1 time ---- 1929909 pairs aligned 0 times concordantly or discordantly; of these: 3859818 mates make up the pairs; of these: 2277113 (59.00%) aligned 0 times 1112418 (28.82%) aligned exactly 1 time 470287 (12.18%) aligned >1 times 96.36% overall alignment rate Time searching: 01:09:55 Overall time: 01:09:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3986338 / 29241050 = 0.1363 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:37:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:37:11: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:37:11: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:37:18: 1000000 INFO @ Tue, 02 Aug 2022 18:37:25: 2000000 INFO @ Tue, 02 Aug 2022 18:37:33: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:37:40: 4000000 INFO @ Tue, 02 Aug 2022 18:37:41: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:37:41: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:37:41: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:37:48: 5000000 INFO @ Tue, 02 Aug 2022 18:37:49: 1000000 INFO @ Tue, 02 Aug 2022 18:37:56: 6000000 INFO @ Tue, 02 Aug 2022 18:37:57: 2000000 INFO @ Tue, 02 Aug 2022 18:38:04: 7000000 INFO @ Tue, 02 Aug 2022 18:38:05: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:38:11: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:38:11: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:38:11: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:38:12: 8000000 INFO @ Tue, 02 Aug 2022 18:38:13: 4000000 INFO @ Tue, 02 Aug 2022 18:38:19: 1000000 INFO @ Tue, 02 Aug 2022 18:38:20: 9000000 INFO @ Tue, 02 Aug 2022 18:38:21: 5000000 INFO @ Tue, 02 Aug 2022 18:38:27: 2000000 INFO @ Tue, 02 Aug 2022 18:38:28: 10000000 INFO @ Tue, 02 Aug 2022 18:38:29: 6000000 INFO @ Tue, 02 Aug 2022 18:38:35: 3000000 INFO @ Tue, 02 Aug 2022 18:38:35: 11000000 INFO @ Tue, 02 Aug 2022 18:38:37: 7000000 INFO @ Tue, 02 Aug 2022 18:38:43: 4000000 INFO @ Tue, 02 Aug 2022 18:38:43: 12000000 INFO @ Tue, 02 Aug 2022 18:38:45: 8000000 INFO @ Tue, 02 Aug 2022 18:38:51: 5000000 INFO @ Tue, 02 Aug 2022 18:38:51: 13000000 INFO @ Tue, 02 Aug 2022 18:38:52: 9000000 INFO @ Tue, 02 Aug 2022 18:38:59: 6000000 INFO @ Tue, 02 Aug 2022 18:38:59: 14000000 INFO @ Tue, 02 Aug 2022 18:39:00: 10000000 INFO @ Tue, 02 Aug 2022 18:39:06: 7000000 INFO @ Tue, 02 Aug 2022 18:39:07: 15000000 INFO @ Tue, 02 Aug 2022 18:39:08: 11000000 INFO @ Tue, 02 Aug 2022 18:39:14: 8000000 INFO @ Tue, 02 Aug 2022 18:39:15: 16000000 INFO @ Tue, 02 Aug 2022 18:39:16: 12000000 INFO @ Tue, 02 Aug 2022 18:39:22: 9000000 INFO @ Tue, 02 Aug 2022 18:39:23: 17000000 INFO @ Tue, 02 Aug 2022 18:39:24: 13000000 INFO @ Tue, 02 Aug 2022 18:39:30: 10000000 INFO @ Tue, 02 Aug 2022 18:39:31: 18000000 INFO @ Tue, 02 Aug 2022 18:39:32: 14000000 INFO @ Tue, 02 Aug 2022 18:39:37: 11000000 INFO @ Tue, 02 Aug 2022 18:39:39: 19000000 INFO @ Tue, 02 Aug 2022 18:39:40: 15000000 INFO @ Tue, 02 Aug 2022 18:39:45: 12000000 INFO @ Tue, 02 Aug 2022 18:39:47: 20000000 INFO @ Tue, 02 Aug 2022 18:39:48: 16000000 INFO @ Tue, 02 Aug 2022 18:39:54: 13000000 INFO @ Tue, 02 Aug 2022 18:39:55: 21000000 INFO @ Tue, 02 Aug 2022 18:39:56: 17000000 INFO @ Tue, 02 Aug 2022 18:40:02: 14000000 INFO @ Tue, 02 Aug 2022 18:40:03: 22000000 INFO @ Tue, 02 Aug 2022 18:40:05: 18000000 INFO @ Tue, 02 Aug 2022 18:40:10: 15000000 INFO @ Tue, 02 Aug 2022 18:40:11: 23000000 INFO @ Tue, 02 Aug 2022 18:40:13: 19000000 INFO @ Tue, 02 Aug 2022 18:40:18: 16000000 INFO @ Tue, 02 Aug 2022 18:40:19: 24000000 INFO @ Tue, 02 Aug 2022 18:40:20: 20000000 INFO @ Tue, 02 Aug 2022 18:40:27: 17000000 INFO @ Tue, 02 Aug 2022 18:40:28: 25000000 INFO @ Tue, 02 Aug 2022 18:40:29: 21000000 INFO @ Tue, 02 Aug 2022 18:40:35: 18000000 INFO @ Tue, 02 Aug 2022 18:40:36: 26000000 INFO @ Tue, 02 Aug 2022 18:40:37: 22000000 INFO @ Tue, 02 Aug 2022 18:40:42: 19000000 INFO @ Tue, 02 Aug 2022 18:40:44: 27000000 INFO @ Tue, 02 Aug 2022 18:40:45: 23000000 INFO @ Tue, 02 Aug 2022 18:40:50: 20000000 INFO @ Tue, 02 Aug 2022 18:40:52: 28000000 INFO @ Tue, 02 Aug 2022 18:40:53: 24000000 INFO @ Tue, 02 Aug 2022 18:40:59: 21000000 INFO @ Tue, 02 Aug 2022 18:41:00: 29000000 INFO @ Tue, 02 Aug 2022 18:41:02: 25000000 INFO @ Tue, 02 Aug 2022 18:41:07: 22000000 INFO @ Tue, 02 Aug 2022 18:41:09: 30000000 INFO @ Tue, 02 Aug 2022 18:41:10: 26000000 INFO @ Tue, 02 Aug 2022 18:41:14: 23000000 INFO @ Tue, 02 Aug 2022 18:41:17: 31000000 INFO @ Tue, 02 Aug 2022 18:41:18: 27000000 INFO @ Tue, 02 Aug 2022 18:41:22: 24000000 INFO @ Tue, 02 Aug 2022 18:41:25: 32000000 INFO @ Tue, 02 Aug 2022 18:41:26: 28000000 INFO @ Tue, 02 Aug 2022 18:41:30: 25000000 INFO @ Tue, 02 Aug 2022 18:41:33: 33000000 INFO @ Tue, 02 Aug 2022 18:41:34: 29000000 INFO @ Tue, 02 Aug 2022 18:41:38: 26000000 INFO @ Tue, 02 Aug 2022 18:41:41: 34000000 INFO @ Tue, 02 Aug 2022 18:41:42: 30000000 INFO @ Tue, 02 Aug 2022 18:41:45: 27000000 INFO @ Tue, 02 Aug 2022 18:41:50: 35000000 INFO @ Tue, 02 Aug 2022 18:41:50: 31000000 INFO @ Tue, 02 Aug 2022 18:41:53: 28000000 INFO @ Tue, 02 Aug 2022 18:41:58: 36000000 INFO @ Tue, 02 Aug 2022 18:41:58: 32000000 INFO @ Tue, 02 Aug 2022 18:42:01: 29000000 INFO @ Tue, 02 Aug 2022 18:42:06: 37000000 INFO @ Tue, 02 Aug 2022 18:42:06: 33000000 INFO @ Tue, 02 Aug 2022 18:42:09: 30000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:42:14: 38000000 INFO @ Tue, 02 Aug 2022 18:42:14: 34000000 INFO @ Tue, 02 Aug 2022 18:42:17: 31000000 INFO @ Tue, 02 Aug 2022 18:42:23: 39000000 INFO @ Tue, 02 Aug 2022 18:42:23: 35000000 INFO @ Tue, 02 Aug 2022 18:42:25: 32000000 INFO @ Tue, 02 Aug 2022 18:42:31: 36000000 INFO @ Tue, 02 Aug 2022 18:42:31: 40000000 INFO @ Tue, 02 Aug 2022 18:42:34: 33000000 INFO @ Tue, 02 Aug 2022 18:42:40: 37000000 INFO @ Tue, 02 Aug 2022 18:42:40: 41000000 INFO @ Tue, 02 Aug 2022 18:42:42: 34000000 INFO @ Tue, 02 Aug 2022 18:42:48: 38000000 INFO @ Tue, 02 Aug 2022 18:42:48: 42000000 INFO @ Tue, 02 Aug 2022 18:42:50: 35000000 INFO @ Tue, 02 Aug 2022 18:42:56: 39000000 INFO @ Tue, 02 Aug 2022 18:42:56: 43000000 INFO @ Tue, 02 Aug 2022 18:42:58: 36000000 INFO @ Tue, 02 Aug 2022 18:43:05: 44000000 INFO @ Tue, 02 Aug 2022 18:43:05: 40000000 INFO @ Tue, 02 Aug 2022 18:43:06: 37000000 INFO @ Tue, 02 Aug 2022 18:43:13: 41000000 INFO @ Tue, 02 Aug 2022 18:43:13: 45000000 INFO @ Tue, 02 Aug 2022 18:43:14: 38000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 18:43:21: 42000000 INFO @ Tue, 02 Aug 2022 18:43:21: 46000000 INFO @ Tue, 02 Aug 2022 18:43:22: 39000000 INFO @ Tue, 02 Aug 2022 18:43:29: 43000000 INFO @ Tue, 02 Aug 2022 18:43:29: 47000000 INFO @ Tue, 02 Aug 2022 18:43:30: 40000000 INFO @ Tue, 02 Aug 2022 18:43:37: 44000000 INFO @ Tue, 02 Aug 2022 18:43:37: 48000000 INFO @ Tue, 02 Aug 2022 18:43:38: 41000000 INFO @ Tue, 02 Aug 2022 18:43:45: 45000000 INFO @ Tue, 02 Aug 2022 18:43:45: 49000000 INFO @ Tue, 02 Aug 2022 18:43:46: 42000000 INFO @ Tue, 02 Aug 2022 18:43:53: 46000000 INFO @ Tue, 02 Aug 2022 18:43:53: 50000000 INFO @ Tue, 02 Aug 2022 18:43:54: 43000000 INFO @ Tue, 02 Aug 2022 18:44:01: 47000000 INFO @ Tue, 02 Aug 2022 18:44:01: 51000000 INFO @ Tue, 02 Aug 2022 18:44:02: 44000000 INFO @ Tue, 02 Aug 2022 18:44:09: 48000000 INFO @ Tue, 02 Aug 2022 18:44:09: 52000000 INFO @ Tue, 02 Aug 2022 18:44:10: 45000000 INFO @ Tue, 02 Aug 2022 18:44:11: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 18:44:11: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 18:44:11: #1 total tags in treatment: 24932429 INFO @ Tue, 02 Aug 2022 18:44:11: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:44:11: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:44:12: #1 tags after filtering in treatment: 17732211 INFO @ Tue, 02 Aug 2022 18:44:12: #1 Redundant rate of treatment: 0.29 INFO @ Tue, 02 Aug 2022 18:44:12: #1 finished! INFO @ Tue, 02 Aug 2022 18:44:12: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:44:12: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:44:13: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 18:44:13: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:44:13: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:44:17: 49000000 INFO @ Tue, 02 Aug 2022 18:44:18: 46000000 INFO @ Tue, 02 Aug 2022 18:44:25: 50000000 INFO @ Tue, 02 Aug 2022 18:44:26: 47000000 INFO @ Tue, 02 Aug 2022 18:44:32: 51000000 INFO @ Tue, 02 Aug 2022 18:44:33: 48000000 INFO @ Tue, 02 Aug 2022 18:44:40: 52000000 INFO @ Tue, 02 Aug 2022 18:44:41: 49000000 INFO @ Tue, 02 Aug 2022 18:44:43: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 18:44:43: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 18:44:43: #1 total tags in treatment: 24932429 INFO @ Tue, 02 Aug 2022 18:44:43: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:44:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:44:43: #1 tags after filtering in treatment: 17732211 INFO @ Tue, 02 Aug 2022 18:44:43: #1 Redundant rate of treatment: 0.29 INFO @ Tue, 02 Aug 2022 18:44:43: #1 finished! INFO @ Tue, 02 Aug 2022 18:44:43: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:44:43: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:44:44: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 18:44:44: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:44:44: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:44:48: 50000000 INFO @ Tue, 02 Aug 2022 18:44:55: 51000000 INFO @ Tue, 02 Aug 2022 18:45:02: 52000000 INFO @ Tue, 02 Aug 2022 18:45:04: #1 tag size is determined as 100 bps INFO @ Tue, 02 Aug 2022 18:45:04: #1 tag size = 100 INFO @ Tue, 02 Aug 2022 18:45:04: #1 total tags in treatment: 24932429 INFO @ Tue, 02 Aug 2022 18:45:04: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:45:04: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:45:04: #1 tags after filtering in treatment: 17732211 INFO @ Tue, 02 Aug 2022 18:45:04: #1 Redundant rate of treatment: 0.29 INFO @ Tue, 02 Aug 2022 18:45:04: #1 finished! INFO @ Tue, 02 Aug 2022 18:45:04: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:45:04: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:45:06: #2 number of paired peaks: 99 WARNING @ Tue, 02 Aug 2022 18:45:06: Too few paired peaks (99) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:45:06: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10855785/SRX10855785.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling