Job ID = 6527543 SRX = SRX1084161 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:00:09 prefetch.2.10.7: 1) Downloading 'SRR2088907'... 2020-06-29T13:00:09 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:02:49 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:02:49 prefetch.2.10.7: 1) 'SRR2088907' was downloaded successfully Read 21974733 spots for SRR2088907/SRR2088907.sra Written 21974733 spots for SRR2088907/SRR2088907.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 00:06:00 21974733 reads; of these: 21974733 (100.00%) were unpaired; of these: 2475250 (11.26%) aligned 0 times 15920513 (72.45%) aligned exactly 1 time 3578970 (16.29%) aligned >1 times 88.74% overall alignment rate Time searching: 00:06:01 Overall time: 00:06:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2849266 / 19499483 = 0.1461 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:20:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:20:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:20:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:20:24: 1000000 INFO @ Mon, 29 Jun 2020 22:20:30: 2000000 INFO @ Mon, 29 Jun 2020 22:20:36: 3000000 INFO @ Mon, 29 Jun 2020 22:20:43: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:20:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:20:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:20:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:20:49: 5000000 INFO @ Mon, 29 Jun 2020 22:20:53: 1000000 INFO @ Mon, 29 Jun 2020 22:20:56: 6000000 INFO @ Mon, 29 Jun 2020 22:20:59: 2000000 INFO @ Mon, 29 Jun 2020 22:21:03: 7000000 INFO @ Mon, 29 Jun 2020 22:21:05: 3000000 INFO @ Mon, 29 Jun 2020 22:21:09: 8000000 INFO @ Mon, 29 Jun 2020 22:21:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:21:16: 9000000 INFO @ Mon, 29 Jun 2020 22:21:16: 5000000 INFO @ Mon, 29 Jun 2020 22:21:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:21:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:21:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:21:22: 6000000 INFO @ Mon, 29 Jun 2020 22:21:23: 10000000 INFO @ Mon, 29 Jun 2020 22:21:23: 1000000 INFO @ Mon, 29 Jun 2020 22:21:28: 7000000 INFO @ Mon, 29 Jun 2020 22:21:29: 11000000 INFO @ Mon, 29 Jun 2020 22:21:30: 2000000 INFO @ Mon, 29 Jun 2020 22:21:34: 8000000 INFO @ Mon, 29 Jun 2020 22:21:36: 3000000 INFO @ Mon, 29 Jun 2020 22:21:36: 12000000 INFO @ Mon, 29 Jun 2020 22:21:40: 9000000 INFO @ Mon, 29 Jun 2020 22:21:42: 4000000 INFO @ Mon, 29 Jun 2020 22:21:43: 13000000 INFO @ Mon, 29 Jun 2020 22:21:46: 10000000 INFO @ Mon, 29 Jun 2020 22:21:48: 5000000 INFO @ Mon, 29 Jun 2020 22:21:51: 14000000 INFO @ Mon, 29 Jun 2020 22:21:52: 11000000 INFO @ Mon, 29 Jun 2020 22:21:55: 6000000 INFO @ Mon, 29 Jun 2020 22:21:58: 15000000 INFO @ Mon, 29 Jun 2020 22:21:59: 12000000 INFO @ Mon, 29 Jun 2020 22:22:01: 7000000 INFO @ Mon, 29 Jun 2020 22:22:05: 16000000 INFO @ Mon, 29 Jun 2020 22:22:05: 13000000 INFO @ Mon, 29 Jun 2020 22:22:07: 8000000 INFO @ Mon, 29 Jun 2020 22:22:09: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 22:22:09: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 22:22:09: #1 total tags in treatment: 16650217 INFO @ Mon, 29 Jun 2020 22:22:09: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:22:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:22:10: #1 tags after filtering in treatment: 16650217 INFO @ Mon, 29 Jun 2020 22:22:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:22:10: #1 finished! INFO @ Mon, 29 Jun 2020 22:22:10: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:22:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:22:11: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:22:11: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:22:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:22:11: 14000000 INFO @ Mon, 29 Jun 2020 22:22:14: 9000000 INFO @ Mon, 29 Jun 2020 22:22:17: 15000000 INFO @ Mon, 29 Jun 2020 22:22:20: 10000000 INFO @ Mon, 29 Jun 2020 22:22:23: 16000000 INFO @ Mon, 29 Jun 2020 22:22:26: 11000000 INFO @ Mon, 29 Jun 2020 22:22:27: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 22:22:27: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 22:22:27: #1 total tags in treatment: 16650217 INFO @ Mon, 29 Jun 2020 22:22:27: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:22:27: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:22:27: #1 tags after filtering in treatment: 16650217 INFO @ Mon, 29 Jun 2020 22:22:27: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:22:27: #1 finished! INFO @ Mon, 29 Jun 2020 22:22:27: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:22:27: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:22:28: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:22:28: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:22:28: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:22:32: 12000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:22:38: 13000000 INFO @ Mon, 29 Jun 2020 22:22:44: 14000000 INFO @ Mon, 29 Jun 2020 22:22:50: 15000000 INFO @ Mon, 29 Jun 2020 22:22:56: 16000000 INFO @ Mon, 29 Jun 2020 22:22:59: #1 tag size is determined as 49 bps INFO @ Mon, 29 Jun 2020 22:22:59: #1 tag size = 49 INFO @ Mon, 29 Jun 2020 22:22:59: #1 total tags in treatment: 16650217 INFO @ Mon, 29 Jun 2020 22:22:59: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:22:59: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:23:00: #1 tags after filtering in treatment: 16650217 INFO @ Mon, 29 Jun 2020 22:23:00: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:23:00: #1 finished! INFO @ Mon, 29 Jun 2020 22:23:00: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:23:00: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:23:01: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:23:01: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:23:01: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX1084161/SRX1084161.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。