Job ID = 14170267 SRX = SRX10706039 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28898286 spots for SRR14352193/SRR14352193.sra Written 28898286 spots for SRR14352193/SRR14352193.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170919 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:02:46 28898286 reads; of these: 28898286 (100.00%) were paired; of these: 2086584 (7.22%) aligned concordantly 0 times 24757992 (85.67%) aligned concordantly exactly 1 time 2053710 (7.11%) aligned concordantly >1 times ---- 2086584 pairs aligned concordantly 0 times; of these: 273998 (13.13%) aligned discordantly 1 time ---- 1812586 pairs aligned 0 times concordantly or discordantly; of these: 3625172 mates make up the pairs; of these: 2694278 (74.32%) aligned 0 times 787572 (21.73%) aligned exactly 1 time 143322 (3.95%) aligned >1 times 95.34% overall alignment rate Time searching: 01:02:46 Overall time: 01:02:46 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6140314 / 20348914 = 0.3018 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:20:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:20:45: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:20:45: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:20:53: 1000000 INFO @ Sat, 11 Dec 2021 07:21:01: 2000000 INFO @ Sat, 11 Dec 2021 07:21:09: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:21:14: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:21:14: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:21:14: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:21:18: 4000000 INFO @ Sat, 11 Dec 2021 07:21:27: 1000000 INFO @ Sat, 11 Dec 2021 07:21:29: 5000000 INFO @ Sat, 11 Dec 2021 07:21:39: 2000000 INFO @ Sat, 11 Dec 2021 07:21:40: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 07:21:44: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 07:21:44: #1 read tag files... INFO @ Sat, 11 Dec 2021 07:21:44: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 07:21:51: 3000000 INFO @ Sat, 11 Dec 2021 07:21:52: 7000000 INFO @ Sat, 11 Dec 2021 07:21:56: 1000000 INFO @ Sat, 11 Dec 2021 07:22:04: 4000000 INFO @ Sat, 11 Dec 2021 07:22:04: 8000000 INFO @ Sat, 11 Dec 2021 07:22:08: 2000000 INFO @ Sat, 11 Dec 2021 07:22:16: 5000000 INFO @ Sat, 11 Dec 2021 07:22:16: 9000000 INFO @ Sat, 11 Dec 2021 07:22:20: 3000000 INFO @ Sat, 11 Dec 2021 07:22:28: 10000000 INFO @ Sat, 11 Dec 2021 07:22:28: 6000000 INFO @ Sat, 11 Dec 2021 07:22:32: 4000000 INFO @ Sat, 11 Dec 2021 07:22:40: 11000000 INFO @ Sat, 11 Dec 2021 07:22:40: 7000000 INFO @ Sat, 11 Dec 2021 07:22:44: 5000000 INFO @ Sat, 11 Dec 2021 07:22:52: 12000000 INFO @ Sat, 11 Dec 2021 07:22:53: 8000000 INFO @ Sat, 11 Dec 2021 07:22:56: 6000000 INFO @ Sat, 11 Dec 2021 07:23:04: 13000000 INFO @ Sat, 11 Dec 2021 07:23:05: 9000000 INFO @ Sat, 11 Dec 2021 07:23:08: 7000000 INFO @ Sat, 11 Dec 2021 07:23:16: 14000000 INFO @ Sat, 11 Dec 2021 07:23:17: 10000000 INFO @ Sat, 11 Dec 2021 07:23:20: 8000000 INFO @ Sat, 11 Dec 2021 07:23:27: 15000000 INFO @ Sat, 11 Dec 2021 07:23:29: 11000000 INFO @ Sat, 11 Dec 2021 07:23:31: 9000000 INFO @ Sat, 11 Dec 2021 07:23:39: 16000000 INFO @ Sat, 11 Dec 2021 07:23:41: 12000000 INFO @ Sat, 11 Dec 2021 07:23:43: 10000000 INFO @ Sat, 11 Dec 2021 07:23:51: 17000000 INFO @ Sat, 11 Dec 2021 07:23:53: 13000000 INFO @ Sat, 11 Dec 2021 07:23:55: 11000000 INFO @ Sat, 11 Dec 2021 07:24:03: 18000000 INFO @ Sat, 11 Dec 2021 07:24:05: 14000000 INFO @ Sat, 11 Dec 2021 07:24:07: 12000000 INFO @ Sat, 11 Dec 2021 07:24:15: 19000000 INFO @ Sat, 11 Dec 2021 07:24:17: 15000000 INFO @ Sat, 11 Dec 2021 07:24:19: 13000000 INFO @ Sat, 11 Dec 2021 07:24:27: 20000000 INFO @ Sat, 11 Dec 2021 07:24:29: 16000000 INFO @ Sat, 11 Dec 2021 07:24:31: 14000000 INFO @ Sat, 11 Dec 2021 07:24:38: 21000000 INFO @ Sat, 11 Dec 2021 07:24:41: 17000000 INFO @ Sat, 11 Dec 2021 07:24:42: 15000000 INFO @ Sat, 11 Dec 2021 07:24:50: 22000000 INFO @ Sat, 11 Dec 2021 07:24:54: 18000000 INFO @ Sat, 11 Dec 2021 07:24:54: 16000000 INFO @ Sat, 11 Dec 2021 07:25:02: 23000000 INFO @ Sat, 11 Dec 2021 07:25:06: 19000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 07:25:06: 17000000 INFO @ Sat, 11 Dec 2021 07:25:14: 24000000 INFO @ Sat, 11 Dec 2021 07:25:18: 18000000 INFO @ Sat, 11 Dec 2021 07:25:18: 20000000 INFO @ Sat, 11 Dec 2021 07:25:25: 25000000 INFO @ Sat, 11 Dec 2021 07:25:30: 19000000 INFO @ Sat, 11 Dec 2021 07:25:32: 21000000 INFO @ Sat, 11 Dec 2021 07:25:37: 26000000 INFO @ Sat, 11 Dec 2021 07:25:41: 20000000 INFO @ Sat, 11 Dec 2021 07:25:44: 22000000 INFO @ Sat, 11 Dec 2021 07:25:49: 27000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 07:25:53: 21000000 INFO @ Sat, 11 Dec 2021 07:25:57: 23000000 INFO @ Sat, 11 Dec 2021 07:26:00: 28000000 INFO @ Sat, 11 Dec 2021 07:26:05: 22000000 INFO @ Sat, 11 Dec 2021 07:26:10: 24000000 INFO @ Sat, 11 Dec 2021 07:26:12: 29000000 INFO @ Sat, 11 Dec 2021 07:26:17: 23000000 INFO @ Sat, 11 Dec 2021 07:26:22: 25000000 INFO @ Sat, 11 Dec 2021 07:26:24: 30000000 INFO @ Sat, 11 Dec 2021 07:26:28: 24000000 INFO @ Sat, 11 Dec 2021 07:26:34: 26000000 INFO @ Sat, 11 Dec 2021 07:26:36: 31000000 INFO @ Sat, 11 Dec 2021 07:26:40: 25000000 INFO @ Sat, 11 Dec 2021 07:26:47: 27000000 INFO @ Sat, 11 Dec 2021 07:26:47: 32000000 INFO @ Sat, 11 Dec 2021 07:26:52: 26000000 INFO @ Sat, 11 Dec 2021 07:26:59: 28000000 INFO @ Sat, 11 Dec 2021 07:26:59: 33000000 INFO @ Sat, 11 Dec 2021 07:27:03: 27000000 INFO @ Sat, 11 Dec 2021 07:27:11: 34000000 INFO @ Sat, 11 Dec 2021 07:27:11: 29000000 INFO @ Sat, 11 Dec 2021 07:27:15: 28000000 INFO @ Sat, 11 Dec 2021 07:27:23: 35000000 INFO @ Sat, 11 Dec 2021 07:27:23: 30000000 INFO @ Sat, 11 Dec 2021 07:27:27: 29000000 INFO @ Sat, 11 Dec 2021 07:27:34: 36000000 INFO @ Sat, 11 Dec 2021 07:27:35: 31000000 INFO @ Sat, 11 Dec 2021 07:27:39: 30000000 INFO @ Sat, 11 Dec 2021 07:27:46: 37000000 INFO @ Sat, 11 Dec 2021 07:27:48: 32000000 INFO @ Sat, 11 Dec 2021 07:27:51: 31000000 INFO @ Sat, 11 Dec 2021 07:27:59: 38000000 INFO @ Sat, 11 Dec 2021 07:28:00: 33000000 INFO @ Sat, 11 Dec 2021 07:28:03: 32000000 INFO @ Sat, 11 Dec 2021 07:28:11: 39000000 INFO @ Sat, 11 Dec 2021 07:28:12: 34000000 INFO @ Sat, 11 Dec 2021 07:28:15: 33000000 INFO @ Sat, 11 Dec 2021 07:28:22: 40000000 INFO @ Sat, 11 Dec 2021 07:28:25: 35000000 INFO @ Sat, 11 Dec 2021 07:28:26: 34000000 INFO @ Sat, 11 Dec 2021 07:28:34: 41000000 INFO @ Sat, 11 Dec 2021 07:28:37: 36000000 INFO @ Sat, 11 Dec 2021 07:28:38: 35000000 INFO @ Sat, 11 Dec 2021 07:28:46: 42000000 INFO @ Sat, 11 Dec 2021 07:28:50: 37000000 INFO @ Sat, 11 Dec 2021 07:28:50: 36000000 INFO @ Sat, 11 Dec 2021 07:28:56: #1 tag size is determined as 148 bps INFO @ Sat, 11 Dec 2021 07:28:56: #1 tag size = 148 INFO @ Sat, 11 Dec 2021 07:28:56: #1 total tags in treatment: 20718999 INFO @ Sat, 11 Dec 2021 07:28:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:28:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:28:56: #1 tags after filtering in treatment: 13478848 INFO @ Sat, 11 Dec 2021 07:28:56: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 11 Dec 2021 07:28:56: #1 finished! INFO @ Sat, 11 Dec 2021 07:28:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:28:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:28:57: #2 number of paired peaks: 2881 INFO @ Sat, 11 Dec 2021 07:28:57: start model_add_line... INFO @ Sat, 11 Dec 2021 07:28:58: start X-correlation... INFO @ Sat, 11 Dec 2021 07:28:58: end of X-cor INFO @ Sat, 11 Dec 2021 07:28:58: #2 finished! INFO @ Sat, 11 Dec 2021 07:28:58: #2 predicted fragment length is 3 bps INFO @ Sat, 11 Dec 2021 07:28:58: #2 alternative fragment length(s) may be 3,13 bps INFO @ Sat, 11 Dec 2021 07:28:58: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.05_model.r WARNING @ Sat, 11 Dec 2021 07:28:58: #2 Since the d (3) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 07:28:58: #2 You may need to consider one of the other alternative d(s): 3,13 WARNING @ Sat, 11 Dec 2021 07:28:58: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 07:28:58: #3 Call peaks... INFO @ Sat, 11 Dec 2021 07:28:58: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 07:29:02: 37000000 INFO @ Sat, 11 Dec 2021 07:29:03: 38000000 INFO @ Sat, 11 Dec 2021 07:29:13: 38000000 INFO @ Sat, 11 Dec 2021 07:29:15: 39000000 INFO @ Sat, 11 Dec 2021 07:29:19: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 07:29:24: 39000000 INFO @ Sat, 11 Dec 2021 07:29:28: 40000000 INFO @ Sat, 11 Dec 2021 07:29:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.05_peaks.xls INFO @ Sat, 11 Dec 2021 07:29:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 07:29:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.05_summits.bed INFO @ Sat, 11 Dec 2021 07:29:30: Done! pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:29:35: 40000000 INFO @ Sat, 11 Dec 2021 07:29:41: 41000000 INFO @ Sat, 11 Dec 2021 07:29:47: 41000000 INFO @ Sat, 11 Dec 2021 07:29:53: 42000000 INFO @ Sat, 11 Dec 2021 07:29:58: 42000000 INFO @ Sat, 11 Dec 2021 07:30:03: #1 tag size is determined as 148 bps INFO @ Sat, 11 Dec 2021 07:30:03: #1 tag size = 148 INFO @ Sat, 11 Dec 2021 07:30:03: #1 total tags in treatment: 20718999 INFO @ Sat, 11 Dec 2021 07:30:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:30:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:30:04: #1 tags after filtering in treatment: 13478848 INFO @ Sat, 11 Dec 2021 07:30:04: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 11 Dec 2021 07:30:04: #1 finished! INFO @ Sat, 11 Dec 2021 07:30:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:30:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:30:05: #2 number of paired peaks: 2881 INFO @ Sat, 11 Dec 2021 07:30:05: start model_add_line... INFO @ Sat, 11 Dec 2021 07:30:05: start X-correlation... INFO @ Sat, 11 Dec 2021 07:30:05: end of X-cor INFO @ Sat, 11 Dec 2021 07:30:05: #2 finished! INFO @ Sat, 11 Dec 2021 07:30:05: #2 predicted fragment length is 3 bps INFO @ Sat, 11 Dec 2021 07:30:05: #2 alternative fragment length(s) may be 3,13 bps INFO @ Sat, 11 Dec 2021 07:30:05: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.10_model.r WARNING @ Sat, 11 Dec 2021 07:30:05: #2 Since the d (3) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 07:30:05: #2 You may need to consider one of the other alternative d(s): 3,13 WARNING @ Sat, 11 Dec 2021 07:30:05: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 07:30:05: #3 Call peaks... INFO @ Sat, 11 Dec 2021 07:30:05: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 07:30:07: #1 tag size is determined as 148 bps INFO @ Sat, 11 Dec 2021 07:30:07: #1 tag size = 148 INFO @ Sat, 11 Dec 2021 07:30:07: #1 total tags in treatment: 20718999 INFO @ Sat, 11 Dec 2021 07:30:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 07:30:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 07:30:07: #1 tags after filtering in treatment: 13478848 INFO @ Sat, 11 Dec 2021 07:30:07: #1 Redundant rate of treatment: 0.35 INFO @ Sat, 11 Dec 2021 07:30:07: #1 finished! INFO @ Sat, 11 Dec 2021 07:30:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 07:30:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 07:30:08: #2 number of paired peaks: 2881 INFO @ Sat, 11 Dec 2021 07:30:08: start model_add_line... INFO @ Sat, 11 Dec 2021 07:30:08: start X-correlation... INFO @ Sat, 11 Dec 2021 07:30:08: end of X-cor INFO @ Sat, 11 Dec 2021 07:30:08: #2 finished! INFO @ Sat, 11 Dec 2021 07:30:08: #2 predicted fragment length is 3 bps INFO @ Sat, 11 Dec 2021 07:30:08: #2 alternative fragment length(s) may be 3,13 bps INFO @ Sat, 11 Dec 2021 07:30:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.20_model.r WARNING @ Sat, 11 Dec 2021 07:30:08: #2 Since the d (3) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 07:30:08: #2 You may need to consider one of the other alternative d(s): 3,13 WARNING @ Sat, 11 Dec 2021 07:30:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 07:30:08: #3 Call peaks... INFO @ Sat, 11 Dec 2021 07:30:08: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 07:30:27: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 07:30:30: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 07:30:37: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.10_peaks.xls INFO @ Sat, 11 Dec 2021 07:30:37: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 07:30:37: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.10_summits.bed INFO @ Sat, 11 Dec 2021 07:30:37: Done! pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 07:30:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.20_peaks.xls INFO @ Sat, 11 Dec 2021 07:30:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 07:30:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10706039/SRX10706039.20_summits.bed INFO @ Sat, 11 Dec 2021 07:30:40: Done! pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) CompletedMACS2peakCalling