Job ID = 16440032 SRX = SRX10447588 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28013014 spots for SRR14073303/SRR14073303.sra Written 28013014 spots for SRR14073303/SRR14073303.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440254 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:44:29 28013014 reads; of these: 28013014 (100.00%) were paired; of these: 3776283 (13.48%) aligned concordantly 0 times 19829096 (70.79%) aligned concordantly exactly 1 time 4407635 (15.73%) aligned concordantly >1 times ---- 3776283 pairs aligned concordantly 0 times; of these: 750506 (19.87%) aligned discordantly 1 time ---- 3025777 pairs aligned 0 times concordantly or discordantly; of these: 6051554 mates make up the pairs; of these: 5265312 (87.01%) aligned 0 times 464407 (7.67%) aligned exactly 1 time 321835 (5.32%) aligned >1 times 90.60% overall alignment rate Time searching: 00:44:29 Overall time: 00:44:29 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 2528048 / 24948322 = 0.1013 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:44:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:44:17: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:44:17: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:44:24: 1000000 INFO @ Tue, 02 Aug 2022 17:44:30: 2000000 INFO @ Tue, 02 Aug 2022 17:44:36: 3000000 INFO @ Tue, 02 Aug 2022 17:44:42: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:44:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:44:46: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:44:46: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:44:47: 5000000 INFO @ Tue, 02 Aug 2022 17:44:53: 1000000 INFO @ Tue, 02 Aug 2022 17:44:54: 6000000 INFO @ Tue, 02 Aug 2022 17:45:00: 2000000 INFO @ Tue, 02 Aug 2022 17:45:01: 7000000 INFO @ Tue, 02 Aug 2022 17:45:07: 3000000 INFO @ Tue, 02 Aug 2022 17:45:07: 8000000 INFO @ Tue, 02 Aug 2022 17:45:14: 9000000 BedGraph に変換中... INFO @ Tue, 02 Aug 2022 17:45:14: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 17:45:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 17:45:16: #1 read tag files... INFO @ Tue, 02 Aug 2022 17:45:16: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 17:45:20: 10000000 INFO @ Tue, 02 Aug 2022 17:45:21: 5000000 INFO @ Tue, 02 Aug 2022 17:45:23: 1000000 INFO @ Tue, 02 Aug 2022 17:45:27: 11000000 INFO @ Tue, 02 Aug 2022 17:45:28: 6000000 INFO @ Tue, 02 Aug 2022 17:45:30: 2000000 INFO @ Tue, 02 Aug 2022 17:45:33: 12000000 INFO @ Tue, 02 Aug 2022 17:45:34: 7000000 INFO @ Tue, 02 Aug 2022 17:45:36: 3000000 INFO @ Tue, 02 Aug 2022 17:45:40: 13000000 INFO @ Tue, 02 Aug 2022 17:45:41: 8000000 INFO @ Tue, 02 Aug 2022 17:45:43: 4000000 INFO @ Tue, 02 Aug 2022 17:45:46: 14000000 INFO @ Tue, 02 Aug 2022 17:45:48: 9000000 INFO @ Tue, 02 Aug 2022 17:45:50: 5000000 INFO @ Tue, 02 Aug 2022 17:45:53: 15000000 INFO @ Tue, 02 Aug 2022 17:45:55: 10000000 INFO @ Tue, 02 Aug 2022 17:45:56: 6000000 INFO @ Tue, 02 Aug 2022 17:46:00: 16000000 INFO @ Tue, 02 Aug 2022 17:46:02: 11000000 INFO @ Tue, 02 Aug 2022 17:46:03: 7000000 INFO @ Tue, 02 Aug 2022 17:46:06: 17000000 INFO @ Tue, 02 Aug 2022 17:46:09: 12000000 INFO @ Tue, 02 Aug 2022 17:46:09: 8000000 INFO @ Tue, 02 Aug 2022 17:46:13: 18000000 INFO @ Tue, 02 Aug 2022 17:46:16: 13000000 INFO @ Tue, 02 Aug 2022 17:46:16: 9000000 INFO @ Tue, 02 Aug 2022 17:46:20: 19000000 INFO @ Tue, 02 Aug 2022 17:46:23: 10000000 INFO @ Tue, 02 Aug 2022 17:46:23: 14000000 INFO @ Tue, 02 Aug 2022 17:46:27: 20000000 INFO @ Tue, 02 Aug 2022 17:46:29: 11000000 INFO @ Tue, 02 Aug 2022 17:46:30: 15000000 INFO @ Tue, 02 Aug 2022 17:46:33: 21000000 INFO @ Tue, 02 Aug 2022 17:46:36: 12000000 INFO @ Tue, 02 Aug 2022 17:46:37: 16000000 INFO @ Tue, 02 Aug 2022 17:46:40: 22000000 INFO @ Tue, 02 Aug 2022 17:46:42: 13000000 INFO @ Tue, 02 Aug 2022 17:46:44: 17000000 INFO @ Tue, 02 Aug 2022 17:46:47: 23000000 INFO @ Tue, 02 Aug 2022 17:46:49: 14000000 INFO @ Tue, 02 Aug 2022 17:46:51: 18000000 INFO @ Tue, 02 Aug 2022 17:46:54: 24000000 INFO @ Tue, 02 Aug 2022 17:46:55: 15000000 INFO @ Tue, 02 Aug 2022 17:46:58: 19000000 INFO @ Tue, 02 Aug 2022 17:47:00: 25000000 INFO @ Tue, 02 Aug 2022 17:47:02: 16000000 INFO @ Tue, 02 Aug 2022 17:47:05: 20000000 INFO @ Tue, 02 Aug 2022 17:47:07: 26000000 INFO @ Tue, 02 Aug 2022 17:47:08: 17000000 INFO @ Tue, 02 Aug 2022 17:47:12: 21000000 INFO @ Tue, 02 Aug 2022 17:47:13: 27000000 INFO @ Tue, 02 Aug 2022 17:47:15: 18000000 INFO @ Tue, 02 Aug 2022 17:47:19: 22000000 INFO @ Tue, 02 Aug 2022 17:47:20: 28000000 INFO @ Tue, 02 Aug 2022 17:47:22: 19000000 INFO @ Tue, 02 Aug 2022 17:47:26: 29000000 INFO @ Tue, 02 Aug 2022 17:47:26: 23000000 INFO @ Tue, 02 Aug 2022 17:47:28: 20000000 INFO @ Tue, 02 Aug 2022 17:47:33: 30000000 INFO @ Tue, 02 Aug 2022 17:47:33: 24000000 INFO @ Tue, 02 Aug 2022 17:47:35: 21000000 INFO @ Tue, 02 Aug 2022 17:47:39: 31000000 INFO @ Tue, 02 Aug 2022 17:47:40: 25000000 INFO @ Tue, 02 Aug 2022 17:47:42: 22000000 INFO @ Tue, 02 Aug 2022 17:47:45: 32000000 INFO @ Tue, 02 Aug 2022 17:47:47: 26000000 INFO @ Tue, 02 Aug 2022 17:47:48: 23000000 INFO @ Tue, 02 Aug 2022 17:47:52: 33000000 INFO @ Tue, 02 Aug 2022 17:47:54: 27000000 INFO @ Tue, 02 Aug 2022 17:47:55: 24000000 INFO @ Tue, 02 Aug 2022 17:47:58: 34000000 INFO @ Tue, 02 Aug 2022 17:48:01: 28000000 INFO @ Tue, 02 Aug 2022 17:48:02: 25000000 INFO @ Tue, 02 Aug 2022 17:48:05: 35000000 INFO @ Tue, 02 Aug 2022 17:48:08: 29000000 INFO @ Tue, 02 Aug 2022 17:48:08: 26000000 INFO @ Tue, 02 Aug 2022 17:48:12: 36000000 INFO @ Tue, 02 Aug 2022 17:48:15: 30000000 INFO @ Tue, 02 Aug 2022 17:48:15: 27000000 INFO @ Tue, 02 Aug 2022 17:48:22: 37000000 INFO @ Tue, 02 Aug 2022 17:48:24: 31000000 INFO @ Tue, 02 Aug 2022 17:48:25: 28000000 INFO @ Tue, 02 Aug 2022 17:48:29: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 17:48:31: 32000000 INFO @ Tue, 02 Aug 2022 17:48:32: 29000000 INFO @ Tue, 02 Aug 2022 17:48:35: 39000000 INFO @ Tue, 02 Aug 2022 17:48:38: 33000000 INFO @ Tue, 02 Aug 2022 17:48:39: 30000000 INFO @ Tue, 02 Aug 2022 17:48:42: 40000000 INFO @ Tue, 02 Aug 2022 17:48:44: 34000000 INFO @ Tue, 02 Aug 2022 17:48:45: 31000000 INFO @ Tue, 02 Aug 2022 17:48:50: 41000000 INFO @ Tue, 02 Aug 2022 17:48:51: 35000000 INFO @ Tue, 02 Aug 2022 17:48:52: 32000000 INFO @ Tue, 02 Aug 2022 17:48:57: 42000000 INFO @ Tue, 02 Aug 2022 17:48:58: 36000000 INFO @ Tue, 02 Aug 2022 17:48:59: 33000000 INFO @ Tue, 02 Aug 2022 17:49:04: 43000000 INFO @ Tue, 02 Aug 2022 17:49:05: 37000000 INFO @ Tue, 02 Aug 2022 17:49:06: 34000000 INFO @ Tue, 02 Aug 2022 17:49:11: 44000000 INFO @ Tue, 02 Aug 2022 17:49:11: 38000000 INFO @ Tue, 02 Aug 2022 17:49:13: 35000000 INFO @ Tue, 02 Aug 2022 17:49:18: 45000000 INFO @ Tue, 02 Aug 2022 17:49:18: 39000000 INFO @ Tue, 02 Aug 2022 17:49:19: 36000000 INFO @ Tue, 02 Aug 2022 17:49:23: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:49:23: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:49:23: #1 total tags in treatment: 21731045 INFO @ Tue, 02 Aug 2022 17:49:23: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:49:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:49:23: #1 tags after filtering in treatment: 14654323 INFO @ Tue, 02 Aug 2022 17:49:23: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 17:49:23: #1 finished! INFO @ Tue, 02 Aug 2022 17:49:23: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:49:23: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:49:24: #2 number of paired peaks: 39 WARNING @ Tue, 02 Aug 2022 17:49:24: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:49:24: Process for pairing-model is terminated! INFO @ Tue, 02 Aug 2022 17:49:24: 40000000 cut: /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:49:26: 37000000 INFO @ Tue, 02 Aug 2022 17:49:31: 41000000 INFO @ Tue, 02 Aug 2022 17:49:33: 38000000 INFO @ Tue, 02 Aug 2022 17:49:37: 42000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 17:49:39: 39000000 INFO @ Tue, 02 Aug 2022 17:49:44: 43000000 INFO @ Tue, 02 Aug 2022 17:49:46: 40000000 INFO @ Tue, 02 Aug 2022 17:49:50: 44000000 INFO @ Tue, 02 Aug 2022 17:49:52: 41000000 INFO @ Tue, 02 Aug 2022 17:49:57: 45000000 INFO @ Tue, 02 Aug 2022 17:49:59: 42000000 INFO @ Tue, 02 Aug 2022 17:50:01: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:50:01: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:50:01: #1 total tags in treatment: 21731045 INFO @ Tue, 02 Aug 2022 17:50:01: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:50:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:50:02: #1 tags after filtering in treatment: 14654323 INFO @ Tue, 02 Aug 2022 17:50:02: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 17:50:02: #1 finished! INFO @ Tue, 02 Aug 2022 17:50:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:50:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:50:03: #2 number of paired peaks: 39 WARNING @ Tue, 02 Aug 2022 17:50:03: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:50:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 17:50:05: 43000000 INFO @ Tue, 02 Aug 2022 17:50:11: 44000000 INFO @ Tue, 02 Aug 2022 17:50:17: 45000000 INFO @ Tue, 02 Aug 2022 17:50:22: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 17:50:22: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 17:50:22: #1 total tags in treatment: 21731045 INFO @ Tue, 02 Aug 2022 17:50:22: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 17:50:22: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 17:50:22: #1 tags after filtering in treatment: 14654323 INFO @ Tue, 02 Aug 2022 17:50:22: #1 Redundant rate of treatment: 0.33 INFO @ Tue, 02 Aug 2022 17:50:22: #1 finished! INFO @ Tue, 02 Aug 2022 17:50:22: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 17:50:22: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 17:50:23: #2 number of paired peaks: 39 WARNING @ Tue, 02 Aug 2022 17:50:23: Too few paired peaks (39) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 17:50:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10447588/SRX10447588.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling