Job ID = 14167099 SRX = SRX10412061 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 37033207 spots for SRR14035725/SRR14035725.sra Written 37033207 spots for SRR14035725/SRR14035725.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168163 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:57:55 37033207 reads; of these: 37033207 (100.00%) were paired; of these: 4401100 (11.88%) aligned concordantly 0 times 22522299 (60.82%) aligned concordantly exactly 1 time 10109808 (27.30%) aligned concordantly >1 times ---- 4401100 pairs aligned concordantly 0 times; of these: 716231 (16.27%) aligned discordantly 1 time ---- 3684869 pairs aligned 0 times concordantly or discordantly; of these: 7369738 mates make up the pairs; of these: 5428892 (73.66%) aligned 0 times 694738 (9.43%) aligned exactly 1 time 1246108 (16.91%) aligned >1 times 92.67% overall alignment rate Time searching: 02:57:55 Overall time: 02:57:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 7536838 / 33272558 = 0.2265 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:23:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:23:08: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:23:08: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:23:19: 1000000 INFO @ Fri, 10 Dec 2021 13:23:31: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:23:37: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:23:37: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:23:37: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:23:44: 3000000 INFO @ Fri, 10 Dec 2021 13:23:51: 1000000 INFO @ Fri, 10 Dec 2021 13:23:57: 4000000 INFO @ Fri, 10 Dec 2021 13:24:04: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 13:24:08: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 13:24:08: #1 read tag files... INFO @ Fri, 10 Dec 2021 13:24:08: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 13:24:10: 5000000 INFO @ Fri, 10 Dec 2021 13:24:17: 3000000 INFO @ Fri, 10 Dec 2021 13:24:21: 1000000 INFO @ Fri, 10 Dec 2021 13:24:23: 6000000 INFO @ Fri, 10 Dec 2021 13:24:31: 4000000 INFO @ Fri, 10 Dec 2021 13:24:34: 2000000 INFO @ Fri, 10 Dec 2021 13:24:36: 7000000 INFO @ Fri, 10 Dec 2021 13:24:44: 5000000 INFO @ Fri, 10 Dec 2021 13:24:47: 3000000 INFO @ Fri, 10 Dec 2021 13:24:49: 8000000 INFO @ Fri, 10 Dec 2021 13:24:57: 6000000 INFO @ Fri, 10 Dec 2021 13:24:59: 4000000 INFO @ Fri, 10 Dec 2021 13:25:02: 9000000 INFO @ Fri, 10 Dec 2021 13:25:10: 7000000 INFO @ Fri, 10 Dec 2021 13:25:12: 5000000 INFO @ Fri, 10 Dec 2021 13:25:15: 10000000 INFO @ Fri, 10 Dec 2021 13:25:23: 8000000 INFO @ Fri, 10 Dec 2021 13:25:25: 6000000 INFO @ Fri, 10 Dec 2021 13:25:27: 11000000 INFO @ Fri, 10 Dec 2021 13:25:37: 9000000 INFO @ Fri, 10 Dec 2021 13:25:37: 7000000 INFO @ Fri, 10 Dec 2021 13:25:40: 12000000 INFO @ Fri, 10 Dec 2021 13:25:50: 10000000 INFO @ Fri, 10 Dec 2021 13:25:50: 8000000 INFO @ Fri, 10 Dec 2021 13:25:53: 13000000 INFO @ Fri, 10 Dec 2021 13:26:03: 9000000 INFO @ Fri, 10 Dec 2021 13:26:03: 11000000 INFO @ Fri, 10 Dec 2021 13:26:06: 14000000 INFO @ Fri, 10 Dec 2021 13:26:16: 10000000 INFO @ Fri, 10 Dec 2021 13:26:16: 12000000 INFO @ Fri, 10 Dec 2021 13:26:19: 15000000 INFO @ Fri, 10 Dec 2021 13:26:29: 11000000 INFO @ Fri, 10 Dec 2021 13:26:29: 13000000 INFO @ Fri, 10 Dec 2021 13:26:32: 16000000 INFO @ Fri, 10 Dec 2021 13:26:42: 12000000 INFO @ Fri, 10 Dec 2021 13:26:42: 14000000 INFO @ Fri, 10 Dec 2021 13:26:44: 17000000 INFO @ Fri, 10 Dec 2021 13:26:55: 13000000 INFO @ Fri, 10 Dec 2021 13:26:55: 15000000 INFO @ Fri, 10 Dec 2021 13:26:57: 18000000 INFO @ Fri, 10 Dec 2021 13:27:07: 14000000 INFO @ Fri, 10 Dec 2021 13:27:08: 16000000 INFO @ Fri, 10 Dec 2021 13:27:10: 19000000 INFO @ Fri, 10 Dec 2021 13:27:20: 15000000 INFO @ Fri, 10 Dec 2021 13:27:21: 17000000 INFO @ Fri, 10 Dec 2021 13:27:23: 20000000 INFO @ Fri, 10 Dec 2021 13:27:33: 16000000 INFO @ Fri, 10 Dec 2021 13:27:34: 18000000 INFO @ Fri, 10 Dec 2021 13:27:36: 21000000 INFO @ Fri, 10 Dec 2021 13:27:45: 17000000 INFO @ Fri, 10 Dec 2021 13:27:47: 19000000 INFO @ Fri, 10 Dec 2021 13:27:49: 22000000 INFO @ Fri, 10 Dec 2021 13:27:57: 18000000 INFO @ Fri, 10 Dec 2021 13:28:01: 20000000 INFO @ Fri, 10 Dec 2021 13:28:02: 23000000 INFO @ Fri, 10 Dec 2021 13:28:10: 19000000 INFO @ Fri, 10 Dec 2021 13:28:14: 21000000 INFO @ Fri, 10 Dec 2021 13:28:15: 24000000 INFO @ Fri, 10 Dec 2021 13:28:23: 20000000 INFO @ Fri, 10 Dec 2021 13:28:27: 22000000 INFO @ Fri, 10 Dec 2021 13:28:28: 25000000 INFO @ Fri, 10 Dec 2021 13:28:35: 21000000 INFO @ Fri, 10 Dec 2021 13:28:41: 23000000 INFO @ Fri, 10 Dec 2021 13:28:41: 26000000 INFO @ Fri, 10 Dec 2021 13:28:48: 22000000 INFO @ Fri, 10 Dec 2021 13:28:53: 24000000 INFO @ Fri, 10 Dec 2021 13:28:54: 27000000 INFO @ Fri, 10 Dec 2021 13:29:01: 23000000 INFO @ Fri, 10 Dec 2021 13:29:06: 25000000 INFO @ Fri, 10 Dec 2021 13:29:07: 28000000 INFO @ Fri, 10 Dec 2021 13:29:13: 24000000 INFO @ Fri, 10 Dec 2021 13:29:19: 29000000 INFO @ Fri, 10 Dec 2021 13:29:19: 26000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 13:29:26: 25000000 INFO @ Fri, 10 Dec 2021 13:29:32: 30000000 INFO @ Fri, 10 Dec 2021 13:29:32: 27000000 INFO @ Fri, 10 Dec 2021 13:29:38: 26000000 INFO @ Fri, 10 Dec 2021 13:29:45: 31000000 INFO @ Fri, 10 Dec 2021 13:29:45: 28000000 INFO @ Fri, 10 Dec 2021 13:29:51: 27000000 INFO @ Fri, 10 Dec 2021 13:29:57: 32000000 INFO @ Fri, 10 Dec 2021 13:29:57: 29000000 INFO @ Fri, 10 Dec 2021 13:30:03: 28000000 INFO @ Fri, 10 Dec 2021 13:30:10: 30000000 INFO @ Fri, 10 Dec 2021 13:30:10: 33000000 INFO @ Fri, 10 Dec 2021 13:30:16: 29000000 INFO @ Fri, 10 Dec 2021 13:30:23: 31000000 INFO @ Fri, 10 Dec 2021 13:30:23: 34000000 INFO @ Fri, 10 Dec 2021 13:30:28: 30000000 INFO @ Fri, 10 Dec 2021 13:30:35: 32000000 INFO @ Fri, 10 Dec 2021 13:30:36: 35000000 INFO @ Fri, 10 Dec 2021 13:30:40: 31000000 INFO @ Fri, 10 Dec 2021 13:30:48: 33000000 INFO @ Fri, 10 Dec 2021 13:30:48: 36000000 INFO @ Fri, 10 Dec 2021 13:30:52: 32000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 13:31:00: 34000000 INFO @ Fri, 10 Dec 2021 13:31:01: 37000000 INFO @ Fri, 10 Dec 2021 13:31:05: 33000000 INFO @ Fri, 10 Dec 2021 13:31:13: 35000000 INFO @ Fri, 10 Dec 2021 13:31:15: 38000000 INFO @ Fri, 10 Dec 2021 13:31:17: 34000000 INFO @ Fri, 10 Dec 2021 13:31:25: 36000000 INFO @ Fri, 10 Dec 2021 13:31:28: 39000000 INFO @ Fri, 10 Dec 2021 13:31:29: 35000000 INFO @ Fri, 10 Dec 2021 13:31:38: 37000000 INFO @ Fri, 10 Dec 2021 13:31:42: 36000000 INFO @ Fri, 10 Dec 2021 13:31:42: 40000000 INFO @ Fri, 10 Dec 2021 13:31:51: 38000000 INFO @ Fri, 10 Dec 2021 13:31:54: 37000000 INFO @ Fri, 10 Dec 2021 13:31:55: 41000000 INFO @ Fri, 10 Dec 2021 13:32:04: 39000000 INFO @ Fri, 10 Dec 2021 13:32:07: 38000000 INFO @ Fri, 10 Dec 2021 13:32:09: 42000000 INFO @ Fri, 10 Dec 2021 13:32:18: 40000000 INFO @ Fri, 10 Dec 2021 13:32:20: 39000000 INFO @ Fri, 10 Dec 2021 13:32:22: 43000000 INFO @ Fri, 10 Dec 2021 13:32:31: 41000000 INFO @ Fri, 10 Dec 2021 13:32:33: 40000000 INFO @ Fri, 10 Dec 2021 13:32:36: 44000000 INFO @ Fri, 10 Dec 2021 13:32:44: 42000000 INFO @ Fri, 10 Dec 2021 13:32:46: 41000000 INFO @ Fri, 10 Dec 2021 13:32:49: 45000000 INFO @ Fri, 10 Dec 2021 13:32:58: 43000000 INFO @ Fri, 10 Dec 2021 13:32:59: 42000000 INFO @ Fri, 10 Dec 2021 13:33:03: 46000000 INFO @ Fri, 10 Dec 2021 13:33:11: 44000000 INFO @ Fri, 10 Dec 2021 13:33:11: 43000000 INFO @ Fri, 10 Dec 2021 13:33:17: 47000000 INFO @ Fri, 10 Dec 2021 13:33:24: 44000000 INFO @ Fri, 10 Dec 2021 13:33:24: 45000000 INFO @ Fri, 10 Dec 2021 13:33:31: 48000000 INFO @ Fri, 10 Dec 2021 13:33:36: 45000000 INFO @ Fri, 10 Dec 2021 13:33:37: 46000000 INFO @ Fri, 10 Dec 2021 13:33:45: 49000000 INFO @ Fri, 10 Dec 2021 13:33:49: 46000000 INFO @ Fri, 10 Dec 2021 13:33:51: 47000000 INFO @ Fri, 10 Dec 2021 13:33:58: 50000000 INFO @ Fri, 10 Dec 2021 13:34:02: 47000000 INFO @ Fri, 10 Dec 2021 13:34:04: 48000000 INFO @ Fri, 10 Dec 2021 13:34:12: 51000000 INFO @ Fri, 10 Dec 2021 13:34:15: 48000000 INFO @ Fri, 10 Dec 2021 13:34:18: 49000000 INFO @ Fri, 10 Dec 2021 13:34:26: 52000000 INFO @ Fri, 10 Dec 2021 13:34:28: 49000000 INFO @ Fri, 10 Dec 2021 13:34:32: 50000000 INFO @ Fri, 10 Dec 2021 13:34:39: 53000000 INFO @ Fri, 10 Dec 2021 13:34:41: 50000000 INFO @ Fri, 10 Dec 2021 13:34:45: 51000000 INFO @ Fri, 10 Dec 2021 13:34:47: #1 tag size is determined as 150 bps INFO @ Fri, 10 Dec 2021 13:34:47: #1 tag size = 150 INFO @ Fri, 10 Dec 2021 13:34:47: #1 total tags in treatment: 25213973 INFO @ Fri, 10 Dec 2021 13:34:47: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:34:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:34:48: #1 tags after filtering in treatment: 22809548 INFO @ Fri, 10 Dec 2021 13:34:48: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 13:34:48: #1 finished! INFO @ Fri, 10 Dec 2021 13:34:48: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:34:48: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:34:49: #2 number of paired peaks: 118 WARNING @ Fri, 10 Dec 2021 13:34:49: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Fri, 10 Dec 2021 13:34:49: start model_add_line... INFO @ Fri, 10 Dec 2021 13:34:49: start X-correlation... INFO @ Fri, 10 Dec 2021 13:34:49: end of X-cor INFO @ Fri, 10 Dec 2021 13:34:49: #2 finished! INFO @ Fri, 10 Dec 2021 13:34:49: #2 predicted fragment length is 203 bps INFO @ Fri, 10 Dec 2021 13:34:49: #2 alternative fragment length(s) may be 203 bps INFO @ Fri, 10 Dec 2021 13:34:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.05_model.r WARNING @ Fri, 10 Dec 2021 13:34:50: #2 Since the d (203) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:34:50: #2 You may need to consider one of the other alternative d(s): 203 WARNING @ Fri, 10 Dec 2021 13:34:50: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:34:50: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:34:50: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:34:54: 51000000 INFO @ Fri, 10 Dec 2021 13:34:58: 52000000 INFO @ Fri, 10 Dec 2021 13:35:07: 52000000 INFO @ Fri, 10 Dec 2021 13:35:10: 53000000 INFO @ Fri, 10 Dec 2021 13:35:18: #1 tag size is determined as 150 bps INFO @ Fri, 10 Dec 2021 13:35:18: #1 tag size = 150 INFO @ Fri, 10 Dec 2021 13:35:18: #1 total tags in treatment: 25213973 INFO @ Fri, 10 Dec 2021 13:35:18: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:35:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:35:18: #1 tags after filtering in treatment: 22809548 INFO @ Fri, 10 Dec 2021 13:35:18: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 13:35:18: #1 finished! INFO @ Fri, 10 Dec 2021 13:35:18: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:35:18: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:35:19: 53000000 INFO @ Fri, 10 Dec 2021 13:35:20: #2 number of paired peaks: 118 WARNING @ Fri, 10 Dec 2021 13:35:20: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Fri, 10 Dec 2021 13:35:20: start model_add_line... INFO @ Fri, 10 Dec 2021 13:35:20: start X-correlation... INFO @ Fri, 10 Dec 2021 13:35:20: end of X-cor INFO @ Fri, 10 Dec 2021 13:35:20: #2 finished! INFO @ Fri, 10 Dec 2021 13:35:20: #2 predicted fragment length is 203 bps INFO @ Fri, 10 Dec 2021 13:35:20: #2 alternative fragment length(s) may be 203 bps INFO @ Fri, 10 Dec 2021 13:35:20: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.10_model.r WARNING @ Fri, 10 Dec 2021 13:35:20: #2 Since the d (203) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:35:20: #2 You may need to consider one of the other alternative d(s): 203 WARNING @ Fri, 10 Dec 2021 13:35:20: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:35:20: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:35:20: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:35:25: #1 tag size is determined as 150 bps INFO @ Fri, 10 Dec 2021 13:35:25: #1 tag size = 150 INFO @ Fri, 10 Dec 2021 13:35:25: #1 total tags in treatment: 25213973 INFO @ Fri, 10 Dec 2021 13:35:25: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 13:35:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 13:35:26: #1 tags after filtering in treatment: 22809548 INFO @ Fri, 10 Dec 2021 13:35:26: #1 Redundant rate of treatment: 0.10 INFO @ Fri, 10 Dec 2021 13:35:26: #1 finished! INFO @ Fri, 10 Dec 2021 13:35:26: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 13:35:26: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 13:35:27: #2 number of paired peaks: 118 WARNING @ Fri, 10 Dec 2021 13:35:27: Fewer paired peaks (118) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 118 pairs to build model! INFO @ Fri, 10 Dec 2021 13:35:27: start model_add_line... INFO @ Fri, 10 Dec 2021 13:35:28: start X-correlation... INFO @ Fri, 10 Dec 2021 13:35:28: end of X-cor INFO @ Fri, 10 Dec 2021 13:35:28: #2 finished! INFO @ Fri, 10 Dec 2021 13:35:28: #2 predicted fragment length is 203 bps INFO @ Fri, 10 Dec 2021 13:35:28: #2 alternative fragment length(s) may be 203 bps INFO @ Fri, 10 Dec 2021 13:35:28: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.20_model.r WARNING @ Fri, 10 Dec 2021 13:35:28: #2 Since the d (203) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 13:35:28: #2 You may need to consider one of the other alternative d(s): 203 WARNING @ Fri, 10 Dec 2021 13:35:28: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 13:35:28: #3 Call peaks... INFO @ Fri, 10 Dec 2021 13:35:28: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 13:35:36: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:35:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.05_peaks.xls INFO @ Fri, 10 Dec 2021 13:35:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:35:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.05_summits.bed INFO @ Fri, 10 Dec 2021 13:35:58: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (7184 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:36:06: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:36:14: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 13:36:28: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.10_peaks.xls INFO @ Fri, 10 Dec 2021 13:36:28: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:36:28: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.10_summits.bed INFO @ Fri, 10 Dec 2021 13:36:28: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (4380 records, 4 fields): 7 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 13:36:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.20_peaks.xls INFO @ Fri, 10 Dec 2021 13:36:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 13:36:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10412061/SRX10412061.20_summits.bed INFO @ Fri, 10 Dec 2021 13:36:36: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (2050 records, 4 fields): 3 millis CompletedMACS2peakCalling