Job ID = 14171832 SRX = SRX10386245 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 30973185 spots for SRR14009310/SRR14009310.sra Written 30973185 spots for SRR14009310/SRR14009310.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172548 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:09:22 30973185 reads; of these: 30973185 (100.00%) were paired; of these: 1861065 (6.01%) aligned concordantly 0 times 18234442 (58.87%) aligned concordantly exactly 1 time 10877678 (35.12%) aligned concordantly >1 times ---- 1861065 pairs aligned concordantly 0 times; of these: 159816 (8.59%) aligned discordantly 1 time ---- 1701249 pairs aligned 0 times concordantly or discordantly; of these: 3402498 mates make up the pairs; of these: 2418070 (71.07%) aligned 0 times 520184 (15.29%) aligned exactly 1 time 464244 (13.64%) aligned >1 times 96.10% overall alignment rate Time searching: 01:09:22 Overall time: 01:09:22 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 28 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4223919 / 29212249 = 0.1446 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:28:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:28:29: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:28:29: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:28:33: 1000000 INFO @ Sat, 11 Dec 2021 14:28:38: 2000000 INFO @ Sat, 11 Dec 2021 14:28:43: 3000000 INFO @ Sat, 11 Dec 2021 14:28:47: 4000000 INFO @ Sat, 11 Dec 2021 14:28:52: 5000000 INFO @ Sat, 11 Dec 2021 14:28:57: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:28:58: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:28:58: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:28:58: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:29:01: 7000000 INFO @ Sat, 11 Dec 2021 14:29:04: 1000000 INFO @ Sat, 11 Dec 2021 14:29:06: 8000000 INFO @ Sat, 11 Dec 2021 14:29:10: 2000000 INFO @ Sat, 11 Dec 2021 14:29:12: 9000000 INFO @ Sat, 11 Dec 2021 14:29:15: 3000000 INFO @ Sat, 11 Dec 2021 14:29:17: 10000000 INFO @ Sat, 11 Dec 2021 14:29:20: 4000000 INFO @ Sat, 11 Dec 2021 14:29:22: 11000000 INFO @ Sat, 11 Dec 2021 14:29:25: 5000000 BedGraph に変換中... INFO @ Sat, 11 Dec 2021 14:29:27: 12000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:29:29: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:29:29: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:29:29: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:29:30: 6000000 INFO @ Sat, 11 Dec 2021 14:29:32: 13000000 INFO @ Sat, 11 Dec 2021 14:29:33: 1000000 INFO @ Sat, 11 Dec 2021 14:29:36: 7000000 INFO @ Sat, 11 Dec 2021 14:29:37: 14000000 INFO @ Sat, 11 Dec 2021 14:29:38: 2000000 INFO @ Sat, 11 Dec 2021 14:29:41: 8000000 INFO @ Sat, 11 Dec 2021 14:29:41: 15000000 INFO @ Sat, 11 Dec 2021 14:29:44: 3000000 INFO @ Sat, 11 Dec 2021 14:29:46: 16000000 INFO @ Sat, 11 Dec 2021 14:29:46: 9000000 INFO @ Sat, 11 Dec 2021 14:29:49: 4000000 INFO @ Sat, 11 Dec 2021 14:29:51: 17000000 INFO @ Sat, 11 Dec 2021 14:29:52: 10000000 INFO @ Sat, 11 Dec 2021 14:29:54: 5000000 INFO @ Sat, 11 Dec 2021 14:29:56: 18000000 INFO @ Sat, 11 Dec 2021 14:29:57: 11000000 INFO @ Sat, 11 Dec 2021 14:29:59: 6000000 INFO @ Sat, 11 Dec 2021 14:30:01: 19000000 INFO @ Sat, 11 Dec 2021 14:30:02: 12000000 INFO @ Sat, 11 Dec 2021 14:30:04: 7000000 INFO @ Sat, 11 Dec 2021 14:30:05: 20000000 INFO @ Sat, 11 Dec 2021 14:30:08: 13000000 INFO @ Sat, 11 Dec 2021 14:30:09: 8000000 INFO @ Sat, 11 Dec 2021 14:30:10: 21000000 INFO @ Sat, 11 Dec 2021 14:30:13: 14000000 INFO @ Sat, 11 Dec 2021 14:30:14: 9000000 INFO @ Sat, 11 Dec 2021 14:30:15: 22000000 INFO @ Sat, 11 Dec 2021 14:30:19: 15000000 INFO @ Sat, 11 Dec 2021 14:30:19: 10000000 INFO @ Sat, 11 Dec 2021 14:30:20: 23000000 INFO @ Sat, 11 Dec 2021 14:30:24: 11000000 INFO @ Sat, 11 Dec 2021 14:30:24: 16000000 INFO @ Sat, 11 Dec 2021 14:30:25: 24000000 INFO @ Sat, 11 Dec 2021 14:30:29: 12000000 INFO @ Sat, 11 Dec 2021 14:30:29: 25000000 INFO @ Sat, 11 Dec 2021 14:30:30: 17000000 INFO @ Sat, 11 Dec 2021 14:30:34: 13000000 INFO @ Sat, 11 Dec 2021 14:30:34: 26000000 INFO @ Sat, 11 Dec 2021 14:30:36: 18000000 INFO @ Sat, 11 Dec 2021 14:30:39: 14000000 INFO @ Sat, 11 Dec 2021 14:30:39: 27000000 INFO @ Sat, 11 Dec 2021 14:30:41: 19000000 INFO @ Sat, 11 Dec 2021 14:30:44: 15000000 INFO @ Sat, 11 Dec 2021 14:30:44: 28000000 INFO @ Sat, 11 Dec 2021 14:30:47: 20000000 INFO @ Sat, 11 Dec 2021 14:30:49: 16000000 INFO @ Sat, 11 Dec 2021 14:30:49: 29000000 INFO @ Sat, 11 Dec 2021 14:30:53: 21000000 INFO @ Sat, 11 Dec 2021 14:30:53: 17000000 INFO @ Sat, 11 Dec 2021 14:30:53: 30000000 INFO @ Sat, 11 Dec 2021 14:30:58: 18000000 INFO @ Sat, 11 Dec 2021 14:30:58: 31000000 INFO @ Sat, 11 Dec 2021 14:30:58: 22000000 INFO @ Sat, 11 Dec 2021 14:31:03: 19000000 INFO @ Sat, 11 Dec 2021 14:31:03: 32000000 INFO @ Sat, 11 Dec 2021 14:31:04: 23000000 INFO @ Sat, 11 Dec 2021 14:31:08: 20000000 INFO @ Sat, 11 Dec 2021 14:31:08: 33000000 INFO @ Sat, 11 Dec 2021 14:31:10: 24000000 INFO @ Sat, 11 Dec 2021 14:31:13: 21000000 INFO @ Sat, 11 Dec 2021 14:31:13: 34000000 INFO @ Sat, 11 Dec 2021 14:31:16: 25000000 INFO @ Sat, 11 Dec 2021 14:31:18: 22000000 INFO @ Sat, 11 Dec 2021 14:31:18: 35000000 INFO @ Sat, 11 Dec 2021 14:31:21: 26000000 INFO @ Sat, 11 Dec 2021 14:31:22: 23000000 INFO @ Sat, 11 Dec 2021 14:31:23: 36000000 INFO @ Sat, 11 Dec 2021 14:31:27: 27000000 INFO @ Sat, 11 Dec 2021 14:31:27: 24000000 INFO @ Sat, 11 Dec 2021 14:31:28: 37000000 INFO @ Sat, 11 Dec 2021 14:31:32: 25000000 INFO @ Sat, 11 Dec 2021 14:31:32: 38000000 INFO @ Sat, 11 Dec 2021 14:31:33: 28000000 INFO @ Sat, 11 Dec 2021 14:31:37: 26000000 INFO @ Sat, 11 Dec 2021 14:31:37: 39000000 INFO @ Sat, 11 Dec 2021 14:31:38: 29000000 INFO @ Sat, 11 Dec 2021 14:31:42: 27000000 INFO @ Sat, 11 Dec 2021 14:31:42: 40000000 INFO @ Sat, 11 Dec 2021 14:31:44: 30000000 INFO @ Sat, 11 Dec 2021 14:31:46: 28000000 INFO @ Sat, 11 Dec 2021 14:31:47: 41000000 INFO @ Sat, 11 Dec 2021 14:31:50: 31000000 INFO @ Sat, 11 Dec 2021 14:31:51: 29000000 INFO @ Sat, 11 Dec 2021 14:31:52: 42000000 INFO @ Sat, 11 Dec 2021 14:31:55: 32000000 INFO @ Sat, 11 Dec 2021 14:31:56: 30000000 INFO @ Sat, 11 Dec 2021 14:31:57: 43000000 INFO @ Sat, 11 Dec 2021 14:32:01: 31000000 INFO @ Sat, 11 Dec 2021 14:32:01: 33000000 INFO @ Sat, 11 Dec 2021 14:32:01: 44000000 INFO @ Sat, 11 Dec 2021 14:32:06: 32000000 INFO @ Sat, 11 Dec 2021 14:32:06: 45000000 INFO @ Sat, 11 Dec 2021 14:32:07: 34000000 INFO @ Sat, 11 Dec 2021 14:32:11: 33000000 INFO @ Sat, 11 Dec 2021 14:32:11: 46000000 INFO @ Sat, 11 Dec 2021 14:32:13: 35000000 INFO @ Sat, 11 Dec 2021 14:32:15: 34000000 INFO @ Sat, 11 Dec 2021 14:32:16: 47000000 INFO @ Sat, 11 Dec 2021 14:32:18: 36000000 INFO @ Sat, 11 Dec 2021 14:32:20: 35000000 INFO @ Sat, 11 Dec 2021 14:32:21: 48000000 INFO @ Sat, 11 Dec 2021 14:32:24: 37000000 INFO @ Sat, 11 Dec 2021 14:32:25: 49000000 INFO @ Sat, 11 Dec 2021 14:32:25: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:32:30: 38000000 INFO @ Sat, 11 Dec 2021 14:32:30: 50000000 INFO @ Sat, 11 Dec 2021 14:32:30: 37000000 INFO @ Sat, 11 Dec 2021 14:32:35: 51000000 INFO @ Sat, 11 Dec 2021 14:32:35: 38000000 INFO @ Sat, 11 Dec 2021 14:32:36: 39000000 INFO @ Sat, 11 Dec 2021 14:32:36: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:32:36: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:32:36: #1 total tags in treatment: 24890344 INFO @ Sat, 11 Dec 2021 14:32:36: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:32:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:32:36: #1 tags after filtering in treatment: 17045868 INFO @ Sat, 11 Dec 2021 14:32:36: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 11 Dec 2021 14:32:36: #1 finished! INFO @ Sat, 11 Dec 2021 14:32:36: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:32:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:32:37: #2 number of paired peaks: 224 WARNING @ Sat, 11 Dec 2021 14:32:37: Fewer paired peaks (224) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 224 pairs to build model! INFO @ Sat, 11 Dec 2021 14:32:37: start model_add_line... INFO @ Sat, 11 Dec 2021 14:32:37: start X-correlation... INFO @ Sat, 11 Dec 2021 14:32:37: end of X-cor INFO @ Sat, 11 Dec 2021 14:32:37: #2 finished! INFO @ Sat, 11 Dec 2021 14:32:37: #2 predicted fragment length is 81 bps INFO @ Sat, 11 Dec 2021 14:32:37: #2 alternative fragment length(s) may be 81 bps INFO @ Sat, 11 Dec 2021 14:32:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.05_model.r WARNING @ Sat, 11 Dec 2021 14:32:37: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 14:32:37: #2 You may need to consider one of the other alternative d(s): 81 WARNING @ Sat, 11 Dec 2021 14:32:37: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 14:32:37: #3 Call peaks... INFO @ Sat, 11 Dec 2021 14:32:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 14:32:40: 39000000 INFO @ Sat, 11 Dec 2021 14:32:41: 40000000 INFO @ Sat, 11 Dec 2021 14:32:45: 40000000 INFO @ Sat, 11 Dec 2021 14:32:47: 41000000 INFO @ Sat, 11 Dec 2021 14:32:50: 41000000 INFO @ Sat, 11 Dec 2021 14:32:53: 42000000 INFO @ Sat, 11 Dec 2021 14:32:55: 42000000 INFO @ Sat, 11 Dec 2021 14:32:58: 43000000 INFO @ Sat, 11 Dec 2021 14:33:00: 43000000 INFO @ Sat, 11 Dec 2021 14:33:04: 44000000 INFO @ Sat, 11 Dec 2021 14:33:05: 44000000 INFO @ Sat, 11 Dec 2021 14:33:08: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 14:33:10: 45000000 INFO @ Sat, 11 Dec 2021 14:33:10: 45000000 INFO @ Sat, 11 Dec 2021 14:33:15: 46000000 INFO @ Sat, 11 Dec 2021 14:33:16: 46000000 INFO @ Sat, 11 Dec 2021 14:33:21: 47000000 INFO @ Sat, 11 Dec 2021 14:33:21: 47000000 INFO @ Sat, 11 Dec 2021 14:33:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.05_peaks.xls INFO @ Sat, 11 Dec 2021 14:33:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 14:33:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.05_summits.bed INFO @ Sat, 11 Dec 2021 14:33:23: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2999 records, 4 fields): 4 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:33:26: 48000000 INFO @ Sat, 11 Dec 2021 14:33:27: 48000000 INFO @ Sat, 11 Dec 2021 14:33:32: 49000000 INFO @ Sat, 11 Dec 2021 14:33:33: 49000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 14:33:38: 50000000 INFO @ Sat, 11 Dec 2021 14:33:38: 50000000 INFO @ Sat, 11 Dec 2021 14:33:43: 51000000 INFO @ Sat, 11 Dec 2021 14:33:44: 51000000 INFO @ Sat, 11 Dec 2021 14:33:44: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:33:44: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:33:44: #1 total tags in treatment: 24890344 INFO @ Sat, 11 Dec 2021 14:33:44: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:33:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:33:45: #1 tags after filtering in treatment: 17045868 INFO @ Sat, 11 Dec 2021 14:33:45: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 11 Dec 2021 14:33:45: #1 finished! INFO @ Sat, 11 Dec 2021 14:33:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:33:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:33:45: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 14:33:45: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 14:33:45: #1 total tags in treatment: 24890344 INFO @ Sat, 11 Dec 2021 14:33:45: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:33:45: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:33:45: #1 tags after filtering in treatment: 17045868 INFO @ Sat, 11 Dec 2021 14:33:45: #1 Redundant rate of treatment: 0.32 INFO @ Sat, 11 Dec 2021 14:33:45: #1 finished! INFO @ Sat, 11 Dec 2021 14:33:45: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:33:45: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:33:46: #2 number of paired peaks: 224 WARNING @ Sat, 11 Dec 2021 14:33:46: Fewer paired peaks (224) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 224 pairs to build model! INFO @ Sat, 11 Dec 2021 14:33:46: start model_add_line... INFO @ Sat, 11 Dec 2021 14:33:46: start X-correlation... INFO @ Sat, 11 Dec 2021 14:33:46: end of X-cor INFO @ Sat, 11 Dec 2021 14:33:46: #2 finished! INFO @ Sat, 11 Dec 2021 14:33:46: #2 predicted fragment length is 81 bps INFO @ Sat, 11 Dec 2021 14:33:46: #2 alternative fragment length(s) may be 81 bps INFO @ Sat, 11 Dec 2021 14:33:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.10_model.r WARNING @ Sat, 11 Dec 2021 14:33:46: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 14:33:46: #2 You may need to consider one of the other alternative d(s): 81 WARNING @ Sat, 11 Dec 2021 14:33:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 14:33:46: #3 Call peaks... INFO @ Sat, 11 Dec 2021 14:33:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 14:33:46: #2 number of paired peaks: 224 WARNING @ Sat, 11 Dec 2021 14:33:46: Fewer paired peaks (224) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 224 pairs to build model! INFO @ Sat, 11 Dec 2021 14:33:46: start model_add_line... INFO @ Sat, 11 Dec 2021 14:33:46: start X-correlation... INFO @ Sat, 11 Dec 2021 14:33:46: end of X-cor INFO @ Sat, 11 Dec 2021 14:33:46: #2 finished! INFO @ Sat, 11 Dec 2021 14:33:46: #2 predicted fragment length is 81 bps INFO @ Sat, 11 Dec 2021 14:33:46: #2 alternative fragment length(s) may be 81 bps INFO @ Sat, 11 Dec 2021 14:33:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.20_model.r WARNING @ Sat, 11 Dec 2021 14:33:46: #2 Since the d (81) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 14:33:46: #2 You may need to consider one of the other alternative d(s): 81 WARNING @ Sat, 11 Dec 2021 14:33:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 14:33:46: #3 Call peaks... INFO @ Sat, 11 Dec 2021 14:33:46: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 14:34:17: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 14:34:17: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 14:34:31: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.20_peaks.xls INFO @ Sat, 11 Dec 2021 14:34:31: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 14:34:31: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.20_summits.bed INFO @ Sat, 11 Dec 2021 14:34:31: Done! pass1 - making usageList (11 chroms): 1 millis pass2 - checking and writing primary data (802 records, 4 fields): 2 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:34:32: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.10_peaks.xls INFO @ Sat, 11 Dec 2021 14:34:32: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 14:34:32: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10386245/SRX10386245.10_summits.bed INFO @ Sat, 11 Dec 2021 14:34:32: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (1199 records, 4 fields): 2 millis CompletedMACS2peakCalling