Job ID = 14171830 SRX = SRX10386244 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18460954 spots for SRR14009309/SRR14009309.sra Written 18460954 spots for SRR14009309/SRR14009309.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172406 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:28:45 18460954 reads; of these: 18460954 (100.00%) were paired; of these: 2889501 (15.65%) aligned concordantly 0 times 12162653 (65.88%) aligned concordantly exactly 1 time 3408800 (18.46%) aligned concordantly >1 times ---- 2889501 pairs aligned concordantly 0 times; of these: 658173 (22.78%) aligned discordantly 1 time ---- 2231328 pairs aligned 0 times concordantly or discordantly; of these: 4462656 mates make up the pairs; of these: 3265860 (73.18%) aligned 0 times 637915 (14.29%) aligned exactly 1 time 558881 (12.52%) aligned >1 times 91.15% overall alignment rate Time searching: 00:28:45 Overall time: 00:28:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 673362 / 16182456 = 0.0416 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:41:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:41:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:41:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:41:08: 1000000 INFO @ Sat, 11 Dec 2021 13:41:14: 2000000 INFO @ Sat, 11 Dec 2021 13:41:19: 3000000 INFO @ Sat, 11 Dec 2021 13:41:25: 4000000 INFO @ Sat, 11 Dec 2021 13:41:30: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:41:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:41:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:41:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:41:36: 6000000 INFO @ Sat, 11 Dec 2021 13:41:39: 1000000 INFO @ Sat, 11 Dec 2021 13:41:42: 7000000 INFO @ Sat, 11 Dec 2021 13:41:45: 2000000 INFO @ Sat, 11 Dec 2021 13:41:48: 8000000 INFO @ Sat, 11 Dec 2021 13:41:52: 3000000 INFO @ Sat, 11 Dec 2021 13:41:54: 9000000 INFO @ Sat, 11 Dec 2021 13:41:58: 4000000 INFO @ Sat, 11 Dec 2021 13:42:00: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:42:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:42:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:42:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:42:04: 5000000 INFO @ Sat, 11 Dec 2021 13:42:07: 11000000 INFO @ Sat, 11 Dec 2021 13:42:09: 1000000 INFO @ Sat, 11 Dec 2021 13:42:10: 6000000 INFO @ Sat, 11 Dec 2021 13:42:13: 12000000 INFO @ Sat, 11 Dec 2021 13:42:16: 2000000 INFO @ Sat, 11 Dec 2021 13:42:17: 7000000 INFO @ Sat, 11 Dec 2021 13:42:19: 13000000 INFO @ Sat, 11 Dec 2021 13:42:22: 3000000 INFO @ Sat, 11 Dec 2021 13:42:23: 8000000 INFO @ Sat, 11 Dec 2021 13:42:26: 14000000 INFO @ Sat, 11 Dec 2021 13:42:28: 4000000 INFO @ Sat, 11 Dec 2021 13:42:30: 9000000 INFO @ Sat, 11 Dec 2021 13:42:32: 15000000 INFO @ Sat, 11 Dec 2021 13:42:35: 5000000 INFO @ Sat, 11 Dec 2021 13:42:36: 10000000 INFO @ Sat, 11 Dec 2021 13:42:38: 16000000 INFO @ Sat, 11 Dec 2021 13:42:41: 6000000 INFO @ Sat, 11 Dec 2021 13:42:43: 11000000 INFO @ Sat, 11 Dec 2021 13:42:44: 17000000 INFO @ Sat, 11 Dec 2021 13:42:47: 7000000 INFO @ Sat, 11 Dec 2021 13:42:49: 12000000 INFO @ Sat, 11 Dec 2021 13:42:51: 18000000 INFO @ Sat, 11 Dec 2021 13:42:54: 8000000 INFO @ Sat, 11 Dec 2021 13:42:56: 13000000 INFO @ Sat, 11 Dec 2021 13:42:57: 19000000 INFO @ Sat, 11 Dec 2021 13:43:00: 9000000 INFO @ Sat, 11 Dec 2021 13:43:02: 14000000 INFO @ Sat, 11 Dec 2021 13:43:03: 20000000 INFO @ Sat, 11 Dec 2021 13:43:06: 10000000 INFO @ Sat, 11 Dec 2021 13:43:08: 15000000 INFO @ Sat, 11 Dec 2021 13:43:10: 21000000 INFO @ Sat, 11 Dec 2021 13:43:13: 11000000 INFO @ Sat, 11 Dec 2021 13:43:15: 16000000 INFO @ Sat, 11 Dec 2021 13:43:16: 22000000 INFO @ Sat, 11 Dec 2021 13:43:19: 12000000 INFO @ Sat, 11 Dec 2021 13:43:21: 17000000 INFO @ Sat, 11 Dec 2021 13:43:22: 23000000 INFO @ Sat, 11 Dec 2021 13:43:26: 13000000 INFO @ Sat, 11 Dec 2021 13:43:27: 18000000 INFO @ Sat, 11 Dec 2021 13:43:29: 24000000 INFO @ Sat, 11 Dec 2021 13:43:32: 14000000 INFO @ Sat, 11 Dec 2021 13:43:34: 19000000 INFO @ Sat, 11 Dec 2021 13:43:35: 25000000 INFO @ Sat, 11 Dec 2021 13:43:38: 15000000 INFO @ Sat, 11 Dec 2021 13:43:40: 20000000 INFO @ Sat, 11 Dec 2021 13:43:42: 26000000 INFO @ Sat, 11 Dec 2021 13:43:45: 16000000 INFO @ Sat, 11 Dec 2021 13:43:47: 21000000 INFO @ Sat, 11 Dec 2021 13:43:48: 27000000 INFO @ Sat, 11 Dec 2021 13:43:51: 17000000 INFO @ Sat, 11 Dec 2021 13:43:53: 22000000 INFO @ Sat, 11 Dec 2021 13:43:55: 28000000 INFO @ Sat, 11 Dec 2021 13:43:57: 18000000 INFO @ Sat, 11 Dec 2021 13:43:59: 23000000 INFO @ Sat, 11 Dec 2021 13:44:02: 29000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:44:04: 19000000 INFO @ Sat, 11 Dec 2021 13:44:06: 24000000 INFO @ Sat, 11 Dec 2021 13:44:08: 30000000 INFO @ Sat, 11 Dec 2021 13:44:10: 20000000 INFO @ Sat, 11 Dec 2021 13:44:13: 25000000 INFO @ Sat, 11 Dec 2021 13:44:15: 31000000 INFO @ Sat, 11 Dec 2021 13:44:17: 21000000 INFO @ Sat, 11 Dec 2021 13:44:20: 26000000 INFO @ Sat, 11 Dec 2021 13:44:22: 32000000 INFO @ Sat, 11 Dec 2021 13:44:24: 22000000 INFO @ Sat, 11 Dec 2021 13:44:24: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:44:24: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:44:24: #1 total tags in treatment: 14903528 INFO @ Sat, 11 Dec 2021 13:44:24: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:44:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:44:25: #1 tags after filtering in treatment: 11546106 INFO @ Sat, 11 Dec 2021 13:44:25: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 11 Dec 2021 13:44:25: #1 finished! INFO @ Sat, 11 Dec 2021 13:44:25: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:44:25: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:44:25: #2 number of paired peaks: 64 WARNING @ Sat, 11 Dec 2021 13:44:25: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:44:25: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:44:27: 27000000 INFO @ Sat, 11 Dec 2021 13:44:30: 23000000 INFO @ Sat, 11 Dec 2021 13:44:34: 28000000 INFO @ Sat, 11 Dec 2021 13:44:37: 24000000 INFO @ Sat, 11 Dec 2021 13:44:40: 29000000 INFO @ Sat, 11 Dec 2021 13:44:43: 25000000 INFO @ Sat, 11 Dec 2021 13:44:47: 30000000 INFO @ Sat, 11 Dec 2021 13:44:50: 26000000 INFO @ Sat, 11 Dec 2021 13:44:53: 31000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 13:44:56: 27000000 INFO @ Sat, 11 Dec 2021 13:45:00: 32000000 INFO @ Sat, 11 Dec 2021 13:45:02: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:45:02: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:45:02: #1 total tags in treatment: 14903528 INFO @ Sat, 11 Dec 2021 13:45:02: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:45:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:45:02: #1 tags after filtering in treatment: 11546106 INFO @ Sat, 11 Dec 2021 13:45:02: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 11 Dec 2021 13:45:02: #1 finished! INFO @ Sat, 11 Dec 2021 13:45:02: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:45:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:45:03: 28000000 INFO @ Sat, 11 Dec 2021 13:45:03: #2 number of paired peaks: 64 WARNING @ Sat, 11 Dec 2021 13:45:03: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:45:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:45:09: 29000000 INFO @ Sat, 11 Dec 2021 13:45:15: 30000000 INFO @ Sat, 11 Dec 2021 13:45:21: 31000000 INFO @ Sat, 11 Dec 2021 13:45:26: 32000000 INFO @ Sat, 11 Dec 2021 13:45:28: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:45:28: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:45:28: #1 total tags in treatment: 14903528 INFO @ Sat, 11 Dec 2021 13:45:28: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:45:28: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:45:28: #1 tags after filtering in treatment: 11546106 INFO @ Sat, 11 Dec 2021 13:45:28: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 11 Dec 2021 13:45:28: #1 finished! INFO @ Sat, 11 Dec 2021 13:45:28: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:45:28: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:45:29: #2 number of paired peaks: 64 WARNING @ Sat, 11 Dec 2021 13:45:29: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:45:29: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386244/SRX10386244.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling