Job ID = 14171761 SRX = SRX10386232 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19477072 spots for SRR14009297/SRR14009297.sra Written 19477072 spots for SRR14009297/SRR14009297.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172310 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:32:19 19477072 reads; of these: 19477072 (100.00%) were paired; of these: 3076123 (15.79%) aligned concordantly 0 times 12107310 (62.16%) aligned concordantly exactly 1 time 4293639 (22.04%) aligned concordantly >1 times ---- 3076123 pairs aligned concordantly 0 times; of these: 384666 (12.50%) aligned discordantly 1 time ---- 2691457 pairs aligned 0 times concordantly or discordantly; of these: 5382914 mates make up the pairs; of these: 3868971 (71.88%) aligned 0 times 697562 (12.96%) aligned exactly 1 time 816381 (15.17%) aligned >1 times 90.07% overall alignment rate Time searching: 00:32:19 Overall time: 00:32:19 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1644492 / 16758715 = 0.0981 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:25:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:25:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:25:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:25:37: 1000000 INFO @ Sat, 11 Dec 2021 13:25:42: 2000000 INFO @ Sat, 11 Dec 2021 13:25:47: 3000000 INFO @ Sat, 11 Dec 2021 13:25:53: 4000000 INFO @ Sat, 11 Dec 2021 13:25:57: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:26:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:26:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:26:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:26:02: 6000000 INFO @ Sat, 11 Dec 2021 13:26:08: 1000000 INFO @ Sat, 11 Dec 2021 13:26:08: 7000000 INFO @ Sat, 11 Dec 2021 13:26:14: 8000000 INFO @ Sat, 11 Dec 2021 13:26:14: 2000000 INFO @ Sat, 11 Dec 2021 13:26:19: 9000000 INFO @ Sat, 11 Dec 2021 13:26:19: 3000000 INFO @ Sat, 11 Dec 2021 13:26:25: 10000000 INFO @ Sat, 11 Dec 2021 13:26:25: 4000000 INFO @ Sat, 11 Dec 2021 13:26:30: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:26:31: 5000000 INFO @ Sat, 11 Dec 2021 13:26:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:26:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:26:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:26:36: 12000000 INFO @ Sat, 11 Dec 2021 13:26:37: 6000000 INFO @ Sat, 11 Dec 2021 13:26:39: 1000000 INFO @ Sat, 11 Dec 2021 13:26:42: 13000000 INFO @ Sat, 11 Dec 2021 13:26:43: 7000000 INFO @ Sat, 11 Dec 2021 13:26:45: 2000000 INFO @ Sat, 11 Dec 2021 13:26:48: 14000000 INFO @ Sat, 11 Dec 2021 13:26:49: 8000000 INFO @ Sat, 11 Dec 2021 13:26:51: 3000000 INFO @ Sat, 11 Dec 2021 13:26:54: 15000000 INFO @ Sat, 11 Dec 2021 13:26:55: 9000000 INFO @ Sat, 11 Dec 2021 13:26:57: 4000000 INFO @ Sat, 11 Dec 2021 13:27:00: 16000000 INFO @ Sat, 11 Dec 2021 13:27:01: 10000000 INFO @ Sat, 11 Dec 2021 13:27:03: 5000000 INFO @ Sat, 11 Dec 2021 13:27:06: 17000000 INFO @ Sat, 11 Dec 2021 13:27:07: 11000000 INFO @ Sat, 11 Dec 2021 13:27:09: 6000000 INFO @ Sat, 11 Dec 2021 13:27:12: 18000000 INFO @ Sat, 11 Dec 2021 13:27:13: 12000000 INFO @ Sat, 11 Dec 2021 13:27:15: 7000000 INFO @ Sat, 11 Dec 2021 13:27:18: 19000000 INFO @ Sat, 11 Dec 2021 13:27:19: 13000000 INFO @ Sat, 11 Dec 2021 13:27:20: 8000000 INFO @ Sat, 11 Dec 2021 13:27:23: 20000000 INFO @ Sat, 11 Dec 2021 13:27:24: 14000000 INFO @ Sat, 11 Dec 2021 13:27:26: 9000000 INFO @ Sat, 11 Dec 2021 13:27:29: 21000000 INFO @ Sat, 11 Dec 2021 13:27:30: 15000000 INFO @ Sat, 11 Dec 2021 13:27:32: 10000000 INFO @ Sat, 11 Dec 2021 13:27:35: 22000000 INFO @ Sat, 11 Dec 2021 13:27:36: 16000000 INFO @ Sat, 11 Dec 2021 13:27:38: 11000000 INFO @ Sat, 11 Dec 2021 13:27:40: 23000000 INFO @ Sat, 11 Dec 2021 13:27:42: 17000000 INFO @ Sat, 11 Dec 2021 13:27:43: 12000000 INFO @ Sat, 11 Dec 2021 13:27:46: 24000000 INFO @ Sat, 11 Dec 2021 13:27:48: 18000000 INFO @ Sat, 11 Dec 2021 13:27:49: 13000000 INFO @ Sat, 11 Dec 2021 13:27:52: 25000000 INFO @ Sat, 11 Dec 2021 13:27:53: 19000000 INFO @ Sat, 11 Dec 2021 13:27:55: 14000000 INFO @ Sat, 11 Dec 2021 13:27:58: 26000000 INFO @ Sat, 11 Dec 2021 13:27:59: 20000000 INFO @ Sat, 11 Dec 2021 13:28:01: 15000000 INFO @ Sat, 11 Dec 2021 13:28:03: 27000000 INFO @ Sat, 11 Dec 2021 13:28:05: 21000000 INFO @ Sat, 11 Dec 2021 13:28:06: 16000000 INFO @ Sat, 11 Dec 2021 13:28:09: 28000000 INFO @ Sat, 11 Dec 2021 13:28:11: 22000000 INFO @ Sat, 11 Dec 2021 13:28:12: 17000000 INFO @ Sat, 11 Dec 2021 13:28:15: 29000000 INFO @ Sat, 11 Dec 2021 13:28:16: 23000000 INFO @ Sat, 11 Dec 2021 13:28:18: 18000000 INFO @ Sat, 11 Dec 2021 13:28:21: 30000000 INFO @ Sat, 11 Dec 2021 13:28:22: 24000000 INFO @ Sat, 11 Dec 2021 13:28:23: 19000000 INFO @ Sat, 11 Dec 2021 13:28:26: 31000000 INFO @ Sat, 11 Dec 2021 13:28:28: 25000000 INFO @ Sat, 11 Dec 2021 13:28:29: 20000000 INFO @ Sat, 11 Dec 2021 13:28:31: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:28:31: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:28:31: #1 total tags in treatment: 14772923 INFO @ Sat, 11 Dec 2021 13:28:31: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:28:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:28:31: #1 tags after filtering in treatment: 13218872 INFO @ Sat, 11 Dec 2021 13:28:31: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 13:28:31: #1 finished! INFO @ Sat, 11 Dec 2021 13:28:31: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:28:31: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:28:32: #2 number of paired peaks: 40 WARNING @ Sat, 11 Dec 2021 13:28:32: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:28:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:28:34: 26000000 INFO @ Sat, 11 Dec 2021 13:28:35: 21000000 INFO @ Sat, 11 Dec 2021 13:28:39: 27000000 INFO @ Sat, 11 Dec 2021 13:28:40: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:28:45: 28000000 INFO @ Sat, 11 Dec 2021 13:28:46: 23000000 INFO @ Sat, 11 Dec 2021 13:28:51: 29000000 INFO @ Sat, 11 Dec 2021 13:28:52: 24000000 INFO @ Sat, 11 Dec 2021 13:28:56: 30000000 INFO @ Sat, 11 Dec 2021 13:28:57: 25000000 INFO @ Sat, 11 Dec 2021 13:29:02: 31000000 INFO @ Sat, 11 Dec 2021 13:29:03: 26000000 INFO @ Sat, 11 Dec 2021 13:29:07: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:29:07: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:29:07: #1 total tags in treatment: 14772923 INFO @ Sat, 11 Dec 2021 13:29:07: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:29:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:29:07: #1 tags after filtering in treatment: 13218872 INFO @ Sat, 11 Dec 2021 13:29:07: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 13:29:07: #1 finished! INFO @ Sat, 11 Dec 2021 13:29:07: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:29:07: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:29:08: #2 number of paired peaks: 40 WARNING @ Sat, 11 Dec 2021 13:29:08: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:29:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:29:08: 27000000 INFO @ Sat, 11 Dec 2021 13:29:14: 28000000 INFO @ Sat, 11 Dec 2021 13:29:19: 29000000 INFO @ Sat, 11 Dec 2021 13:29:24: 30000000 INFO @ Sat, 11 Dec 2021 13:29:29: 31000000 INFO @ Sat, 11 Dec 2021 13:29:33: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:29:33: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:29:33: #1 total tags in treatment: 14772923 INFO @ Sat, 11 Dec 2021 13:29:33: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:29:33: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:29:33: #1 tags after filtering in treatment: 13218872 INFO @ Sat, 11 Dec 2021 13:29:33: #1 Redundant rate of treatment: 0.11 INFO @ Sat, 11 Dec 2021 13:29:33: #1 finished! INFO @ Sat, 11 Dec 2021 13:29:33: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:29:33: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:29:34: #2 number of paired peaks: 40 WARNING @ Sat, 11 Dec 2021 13:29:34: Too few paired peaks (40) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 13:29:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386232/SRX10386232.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。