Job ID = 14171656 SRX = SRX10386216 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26021124 spots for SRR14009281/SRR14009281.sra Written 26021124 spots for SRR14009281/SRR14009281.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172302 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:56:45 26021124 reads; of these: 26021124 (100.00%) were paired; of these: 3360561 (12.91%) aligned concordantly 0 times 13551882 (52.08%) aligned concordantly exactly 1 time 9108681 (35.00%) aligned concordantly >1 times ---- 3360561 pairs aligned concordantly 0 times; of these: 374004 (11.13%) aligned discordantly 1 time ---- 2986557 pairs aligned 0 times concordantly or discordantly; of these: 5973114 mates make up the pairs; of these: 4845801 (81.13%) aligned 0 times 508451 (8.51%) aligned exactly 1 time 618862 (10.36%) aligned >1 times 90.69% overall alignment rate Time searching: 00:56:45 Overall time: 00:56:45 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4189683 / 22969385 = 0.1824 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:24:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:24:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:24:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:24:54: 1000000 INFO @ Sat, 11 Dec 2021 13:24:59: 2000000 INFO @ Sat, 11 Dec 2021 13:25:05: 3000000 INFO @ Sat, 11 Dec 2021 13:25:10: 4000000 INFO @ Sat, 11 Dec 2021 13:25:15: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:25:19: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:25:19: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:25:19: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:25:20: 6000000 INFO @ Sat, 11 Dec 2021 13:25:24: 1000000 INFO @ Sat, 11 Dec 2021 13:25:25: 7000000 INFO @ Sat, 11 Dec 2021 13:25:28: 2000000 INFO @ Sat, 11 Dec 2021 13:25:30: 8000000 INFO @ Sat, 11 Dec 2021 13:25:33: 3000000 INFO @ Sat, 11 Dec 2021 13:25:35: 9000000 INFO @ Sat, 11 Dec 2021 13:25:37: 4000000 INFO @ Sat, 11 Dec 2021 13:25:40: 10000000 INFO @ Sat, 11 Dec 2021 13:25:42: 5000000 INFO @ Sat, 11 Dec 2021 13:25:46: 11000000 INFO @ Sat, 11 Dec 2021 13:25:46: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 13:25:49: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 13:25:49: #1 read tag files... INFO @ Sat, 11 Dec 2021 13:25:49: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 13:25:51: 7000000 INFO @ Sat, 11 Dec 2021 13:25:51: 12000000 INFO @ Sat, 11 Dec 2021 13:25:54: 1000000 INFO @ Sat, 11 Dec 2021 13:25:55: 8000000 INFO @ Sat, 11 Dec 2021 13:25:56: 13000000 INFO @ Sat, 11 Dec 2021 13:25:58: 2000000 INFO @ Sat, 11 Dec 2021 13:26:00: 9000000 INFO @ Sat, 11 Dec 2021 13:26:01: 14000000 INFO @ Sat, 11 Dec 2021 13:26:03: 3000000 INFO @ Sat, 11 Dec 2021 13:26:04: 10000000 INFO @ Sat, 11 Dec 2021 13:26:06: 15000000 INFO @ Sat, 11 Dec 2021 13:26:07: 4000000 INFO @ Sat, 11 Dec 2021 13:26:09: 11000000 INFO @ Sat, 11 Dec 2021 13:26:11: 16000000 INFO @ Sat, 11 Dec 2021 13:26:12: 5000000 INFO @ Sat, 11 Dec 2021 13:26:13: 12000000 INFO @ Sat, 11 Dec 2021 13:26:16: 6000000 INFO @ Sat, 11 Dec 2021 13:26:16: 17000000 INFO @ Sat, 11 Dec 2021 13:26:18: 13000000 INFO @ Sat, 11 Dec 2021 13:26:21: 7000000 INFO @ Sat, 11 Dec 2021 13:26:22: 18000000 INFO @ Sat, 11 Dec 2021 13:26:22: 14000000 INFO @ Sat, 11 Dec 2021 13:26:25: 8000000 INFO @ Sat, 11 Dec 2021 13:26:27: 19000000 INFO @ Sat, 11 Dec 2021 13:26:27: 15000000 INFO @ Sat, 11 Dec 2021 13:26:30: 9000000 INFO @ Sat, 11 Dec 2021 13:26:31: 16000000 INFO @ Sat, 11 Dec 2021 13:26:32: 20000000 INFO @ Sat, 11 Dec 2021 13:26:34: 10000000 INFO @ Sat, 11 Dec 2021 13:26:36: 17000000 INFO @ Sat, 11 Dec 2021 13:26:37: 21000000 INFO @ Sat, 11 Dec 2021 13:26:39: 11000000 INFO @ Sat, 11 Dec 2021 13:26:40: 18000000 INFO @ Sat, 11 Dec 2021 13:26:42: 22000000 INFO @ Sat, 11 Dec 2021 13:26:43: 12000000 INFO @ Sat, 11 Dec 2021 13:26:45: 19000000 INFO @ Sat, 11 Dec 2021 13:26:47: 23000000 INFO @ Sat, 11 Dec 2021 13:26:48: 13000000 INFO @ Sat, 11 Dec 2021 13:26:49: 20000000 INFO @ Sat, 11 Dec 2021 13:26:52: 14000000 INFO @ Sat, 11 Dec 2021 13:26:52: 24000000 INFO @ Sat, 11 Dec 2021 13:26:54: 21000000 INFO @ Sat, 11 Dec 2021 13:26:57: 15000000 INFO @ Sat, 11 Dec 2021 13:26:58: 25000000 INFO @ Sat, 11 Dec 2021 13:26:58: 22000000 INFO @ Sat, 11 Dec 2021 13:27:01: 16000000 INFO @ Sat, 11 Dec 2021 13:27:03: 26000000 INFO @ Sat, 11 Dec 2021 13:27:03: 23000000 INFO @ Sat, 11 Dec 2021 13:27:06: 17000000 INFO @ Sat, 11 Dec 2021 13:27:07: 24000000 INFO @ Sat, 11 Dec 2021 13:27:08: 27000000 INFO @ Sat, 11 Dec 2021 13:27:10: 18000000 INFO @ Sat, 11 Dec 2021 13:27:12: 25000000 INFO @ Sat, 11 Dec 2021 13:27:13: 28000000 INFO @ Sat, 11 Dec 2021 13:27:15: 19000000 INFO @ Sat, 11 Dec 2021 13:27:16: 26000000 INFO @ Sat, 11 Dec 2021 13:27:18: 29000000 INFO @ Sat, 11 Dec 2021 13:27:19: 20000000 INFO @ Sat, 11 Dec 2021 13:27:21: 27000000 INFO @ Sat, 11 Dec 2021 13:27:24: 30000000 INFO @ Sat, 11 Dec 2021 13:27:24: 21000000 INFO @ Sat, 11 Dec 2021 13:27:26: 28000000 INFO @ Sat, 11 Dec 2021 13:27:28: 22000000 INFO @ Sat, 11 Dec 2021 13:27:29: 31000000 INFO @ Sat, 11 Dec 2021 13:27:30: 29000000 INFO @ Sat, 11 Dec 2021 13:27:33: 23000000 INFO @ Sat, 11 Dec 2021 13:27:34: 32000000 INFO @ Sat, 11 Dec 2021 13:27:35: 30000000 INFO @ Sat, 11 Dec 2021 13:27:37: 24000000 INFO @ Sat, 11 Dec 2021 13:27:39: 33000000 INFO @ Sat, 11 Dec 2021 13:27:39: 31000000 INFO @ Sat, 11 Dec 2021 13:27:42: 25000000 INFO @ Sat, 11 Dec 2021 13:27:44: 32000000 INFO @ Sat, 11 Dec 2021 13:27:44: 34000000 INFO @ Sat, 11 Dec 2021 13:27:46: 26000000 INFO @ Sat, 11 Dec 2021 13:27:48: 33000000 INFO @ Sat, 11 Dec 2021 13:27:50: 35000000 INFO @ Sat, 11 Dec 2021 13:27:51: 27000000 INFO @ Sat, 11 Dec 2021 13:27:53: 34000000 INFO @ Sat, 11 Dec 2021 13:27:55: 36000000 INFO @ Sat, 11 Dec 2021 13:27:55: 28000000 INFO @ Sat, 11 Dec 2021 13:27:58: 35000000 INFO @ Sat, 11 Dec 2021 13:28:00: 29000000 INFO @ Sat, 11 Dec 2021 13:28:00: 37000000 INFO @ Sat, 11 Dec 2021 13:28:02: 36000000 INFO @ Sat, 11 Dec 2021 13:28:04: 30000000 INFO @ Sat, 11 Dec 2021 13:28:05: 38000000 INFO @ Sat, 11 Dec 2021 13:28:07: 37000000 INFO @ Sat, 11 Dec 2021 13:28:09: 31000000 INFO @ Sat, 11 Dec 2021 13:28:10: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:28:10: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:28:10: #1 total tags in treatment: 18481046 INFO @ Sat, 11 Dec 2021 13:28:10: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:28:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:28:10: #1 tags after filtering in treatment: 14229531 INFO @ Sat, 11 Dec 2021 13:28:10: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 11 Dec 2021 13:28:10: #1 finished! INFO @ Sat, 11 Dec 2021 13:28:10: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:28:10: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:28:11: #2 number of paired peaks: 306 WARNING @ Sat, 11 Dec 2021 13:28:11: Fewer paired peaks (306) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 306 pairs to build model! INFO @ Sat, 11 Dec 2021 13:28:11: start model_add_line... INFO @ Sat, 11 Dec 2021 13:28:11: start X-correlation... INFO @ Sat, 11 Dec 2021 13:28:11: end of X-cor INFO @ Sat, 11 Dec 2021 13:28:11: #2 finished! INFO @ Sat, 11 Dec 2021 13:28:11: #2 predicted fragment length is 98 bps INFO @ Sat, 11 Dec 2021 13:28:11: #2 alternative fragment length(s) may be 98 bps INFO @ Sat, 11 Dec 2021 13:28:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.05_model.r WARNING @ Sat, 11 Dec 2021 13:28:11: #2 Since the d (98) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 13:28:11: #2 You may need to consider one of the other alternative d(s): 98 WARNING @ Sat, 11 Dec 2021 13:28:11: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 13:28:11: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:28:11: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:28:11: 38000000 INFO @ Sat, 11 Dec 2021 13:28:13: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 13:28:15: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:28:15: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:28:15: #1 total tags in treatment: 18481046 INFO @ Sat, 11 Dec 2021 13:28:15: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:28:15: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:28:15: #1 tags after filtering in treatment: 14229531 INFO @ Sat, 11 Dec 2021 13:28:15: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 11 Dec 2021 13:28:15: #1 finished! INFO @ Sat, 11 Dec 2021 13:28:15: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:28:15: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:28:16: #2 number of paired peaks: 306 WARNING @ Sat, 11 Dec 2021 13:28:16: Fewer paired peaks (306) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 306 pairs to build model! INFO @ Sat, 11 Dec 2021 13:28:16: start model_add_line... INFO @ Sat, 11 Dec 2021 13:28:16: start X-correlation... INFO @ Sat, 11 Dec 2021 13:28:16: end of X-cor INFO @ Sat, 11 Dec 2021 13:28:16: #2 finished! INFO @ Sat, 11 Dec 2021 13:28:16: #2 predicted fragment length is 98 bps INFO @ Sat, 11 Dec 2021 13:28:16: #2 alternative fragment length(s) may be 98 bps INFO @ Sat, 11 Dec 2021 13:28:16: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.10_model.r WARNING @ Sat, 11 Dec 2021 13:28:16: #2 Since the d (98) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 13:28:16: #2 You may need to consider one of the other alternative d(s): 98 WARNING @ Sat, 11 Dec 2021 13:28:16: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 13:28:16: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:28:16: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:28:18: 33000000 INFO @ Sat, 11 Dec 2021 13:28:22: 34000000 INFO @ Sat, 11 Dec 2021 13:28:27: 35000000 INFO @ Sat, 11 Dec 2021 13:28:31: 36000000 INFO @ Sat, 11 Dec 2021 13:28:35: 37000000 INFO @ Sat, 11 Dec 2021 13:28:39: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 13:28:40: 38000000 INFO @ Sat, 11 Dec 2021 13:28:43: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 13:28:43: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 13:28:43: #1 total tags in treatment: 18481046 INFO @ Sat, 11 Dec 2021 13:28:43: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 13:28:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 13:28:44: #1 tags after filtering in treatment: 14229531 INFO @ Sat, 11 Dec 2021 13:28:44: #1 Redundant rate of treatment: 0.23 INFO @ Sat, 11 Dec 2021 13:28:44: #1 finished! INFO @ Sat, 11 Dec 2021 13:28:44: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 13:28:44: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 13:28:44: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 13:28:44: #2 number of paired peaks: 306 WARNING @ Sat, 11 Dec 2021 13:28:44: Fewer paired peaks (306) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 306 pairs to build model! INFO @ Sat, 11 Dec 2021 13:28:44: start model_add_line... INFO @ Sat, 11 Dec 2021 13:28:44: start X-correlation... INFO @ Sat, 11 Dec 2021 13:28:44: end of X-cor INFO @ Sat, 11 Dec 2021 13:28:44: #2 finished! INFO @ Sat, 11 Dec 2021 13:28:44: #2 predicted fragment length is 98 bps INFO @ Sat, 11 Dec 2021 13:28:44: #2 alternative fragment length(s) may be 98 bps INFO @ Sat, 11 Dec 2021 13:28:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.20_model.r WARNING @ Sat, 11 Dec 2021 13:28:44: #2 Since the d (98) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 13:28:44: #2 You may need to consider one of the other alternative d(s): 98 WARNING @ Sat, 11 Dec 2021 13:28:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 13:28:44: #3 Call peaks... INFO @ Sat, 11 Dec 2021 13:28:44: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 13:28:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.05_peaks.xls INFO @ Sat, 11 Dec 2021 13:28:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:28:53: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.05_summits.bed INFO @ Sat, 11 Dec 2021 13:28:53: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2074 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:28:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.10_peaks.xls INFO @ Sat, 11 Dec 2021 13:28:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:28:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.10_summits.bed INFO @ Sat, 11 Dec 2021 13:28:58: Done! pass1 - making usageList (12 chroms): 1 millis pass2 - checking and writing primary data (1291 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 13:29:11: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 13:29:24: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.20_peaks.xls INFO @ Sat, 11 Dec 2021 13:29:24: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 13:29:24: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10386216/SRX10386216.20_summits.bed INFO @ Sat, 11 Dec 2021 13:29:24: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (858 records, 4 fields): 4 millis CompletedMACS2peakCalling