Job ID = 14172073 SRX = SRX10386198 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 26126999 spots for SRR14009263/SRR14009263.sra Written 26126999 spots for SRR14009263/SRR14009263.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172783 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:07:41 26126999 reads; of these: 26126999 (100.00%) were paired; of these: 4446504 (17.02%) aligned concordantly 0 times 14843301 (56.81%) aligned concordantly exactly 1 time 6837194 (26.17%) aligned concordantly >1 times ---- 4446504 pairs aligned concordantly 0 times; of these: 387233 (8.71%) aligned discordantly 1 time ---- 4059271 pairs aligned 0 times concordantly or discordantly; of these: 8118542 mates make up the pairs; of these: 7162303 (88.22%) aligned 0 times 499243 (6.15%) aligned exactly 1 time 456996 (5.63%) aligned >1 times 86.29% overall alignment rate Time searching: 01:07:42 Overall time: 01:07:42 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 20 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 4066068 / 22007415 = 0.1848 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:23:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:23:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:23:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:23:43: 1000000 INFO @ Sat, 11 Dec 2021 15:23:54: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:24:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:24:01: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:24:01: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:24:04: 3000000 INFO @ Sat, 11 Dec 2021 15:24:11: 1000000 INFO @ Sat, 11 Dec 2021 15:24:13: 4000000 INFO @ Sat, 11 Dec 2021 15:24:21: 2000000 INFO @ Sat, 11 Dec 2021 15:24:23: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 15:24:31: 3000000 INFO @ Sat, 11 Dec 2021 15:24:31: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 15:24:31: #1 read tag files... INFO @ Sat, 11 Dec 2021 15:24:31: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 15:24:33: 6000000 INFO @ Sat, 11 Dec 2021 15:24:41: 4000000 INFO @ Sat, 11 Dec 2021 15:24:41: 1000000 INFO @ Sat, 11 Dec 2021 15:24:43: 7000000 INFO @ Sat, 11 Dec 2021 15:24:51: 5000000 INFO @ Sat, 11 Dec 2021 15:24:52: 2000000 INFO @ Sat, 11 Dec 2021 15:24:52: 8000000 INFO @ Sat, 11 Dec 2021 15:25:01: 6000000 INFO @ Sat, 11 Dec 2021 15:25:02: 9000000 INFO @ Sat, 11 Dec 2021 15:25:02: 3000000 INFO @ Sat, 11 Dec 2021 15:25:11: 7000000 INFO @ Sat, 11 Dec 2021 15:25:12: 10000000 INFO @ Sat, 11 Dec 2021 15:25:12: 4000000 INFO @ Sat, 11 Dec 2021 15:25:21: 8000000 INFO @ Sat, 11 Dec 2021 15:25:21: 11000000 INFO @ Sat, 11 Dec 2021 15:25:22: 5000000 INFO @ Sat, 11 Dec 2021 15:25:31: 9000000 INFO @ Sat, 11 Dec 2021 15:25:31: 12000000 INFO @ Sat, 11 Dec 2021 15:25:32: 6000000 INFO @ Sat, 11 Dec 2021 15:25:41: 13000000 INFO @ Sat, 11 Dec 2021 15:25:41: 10000000 INFO @ Sat, 11 Dec 2021 15:25:42: 7000000 INFO @ Sat, 11 Dec 2021 15:25:50: 14000000 INFO @ Sat, 11 Dec 2021 15:25:52: 8000000 INFO @ Sat, 11 Dec 2021 15:25:52: 11000000 INFO @ Sat, 11 Dec 2021 15:26:00: 15000000 INFO @ Sat, 11 Dec 2021 15:26:01: 9000000 INFO @ Sat, 11 Dec 2021 15:26:02: 12000000 INFO @ Sat, 11 Dec 2021 15:26:09: 16000000 INFO @ Sat, 11 Dec 2021 15:26:11: 10000000 INFO @ Sat, 11 Dec 2021 15:26:12: 13000000 INFO @ Sat, 11 Dec 2021 15:26:19: 17000000 INFO @ Sat, 11 Dec 2021 15:26:21: 11000000 INFO @ Sat, 11 Dec 2021 15:26:22: 14000000 INFO @ Sat, 11 Dec 2021 15:26:29: 18000000 INFO @ Sat, 11 Dec 2021 15:26:31: 12000000 INFO @ Sat, 11 Dec 2021 15:26:32: 15000000 INFO @ Sat, 11 Dec 2021 15:26:38: 19000000 INFO @ Sat, 11 Dec 2021 15:26:41: 13000000 INFO @ Sat, 11 Dec 2021 15:26:42: 16000000 INFO @ Sat, 11 Dec 2021 15:26:48: 20000000 INFO @ Sat, 11 Dec 2021 15:26:51: 14000000 INFO @ Sat, 11 Dec 2021 15:26:52: 17000000 INFO @ Sat, 11 Dec 2021 15:26:58: 21000000 INFO @ Sat, 11 Dec 2021 15:27:00: 15000000 INFO @ Sat, 11 Dec 2021 15:27:02: 18000000 INFO @ Sat, 11 Dec 2021 15:27:07: 22000000 INFO @ Sat, 11 Dec 2021 15:27:09: 16000000 INFO @ Sat, 11 Dec 2021 15:27:12: 19000000 INFO @ Sat, 11 Dec 2021 15:27:17: 23000000 INFO @ Sat, 11 Dec 2021 15:27:18: 17000000 INFO @ Sat, 11 Dec 2021 15:27:22: 20000000 INFO @ Sat, 11 Dec 2021 15:27:26: 24000000 INFO @ Sat, 11 Dec 2021 15:27:27: 18000000 INFO @ Sat, 11 Dec 2021 15:27:31: 21000000 INFO @ Sat, 11 Dec 2021 15:27:36: 25000000 INFO @ Sat, 11 Dec 2021 15:27:36: 19000000 INFO @ Sat, 11 Dec 2021 15:27:41: 22000000 INFO @ Sat, 11 Dec 2021 15:27:45: 20000000 INFO @ Sat, 11 Dec 2021 15:27:45: 26000000 INFO @ Sat, 11 Dec 2021 15:27:50: 23000000 INFO @ Sat, 11 Dec 2021 15:27:54: 21000000 INFO @ Sat, 11 Dec 2021 15:27:55: 27000000 INFO @ Sat, 11 Dec 2021 15:27:59: 24000000 INFO @ Sat, 11 Dec 2021 15:28:03: 22000000 INFO @ Sat, 11 Dec 2021 15:28:04: 28000000 INFO @ Sat, 11 Dec 2021 15:28:09: 25000000 INFO @ Sat, 11 Dec 2021 15:28:12: 23000000 INFO @ Sat, 11 Dec 2021 15:28:14: 29000000 INFO @ Sat, 11 Dec 2021 15:28:18: 26000000 INFO @ Sat, 11 Dec 2021 15:28:21: 24000000 INFO @ Sat, 11 Dec 2021 15:28:23: 30000000 INFO @ Sat, 11 Dec 2021 15:28:27: 27000000 INFO @ Sat, 11 Dec 2021 15:28:30: 25000000 INFO @ Sat, 11 Dec 2021 15:28:33: 31000000 INFO @ Sat, 11 Dec 2021 15:28:37: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 15:28:39: 26000000 INFO @ Sat, 11 Dec 2021 15:28:42: 32000000 INFO @ Sat, 11 Dec 2021 15:28:46: 29000000 INFO @ Sat, 11 Dec 2021 15:28:48: 27000000 INFO @ Sat, 11 Dec 2021 15:28:52: 33000000 INFO @ Sat, 11 Dec 2021 15:28:56: 30000000 INFO @ Sat, 11 Dec 2021 15:28:57: 28000000 INFO @ Sat, 11 Dec 2021 15:29:01: 34000000 INFO @ Sat, 11 Dec 2021 15:29:05: 31000000 INFO @ Sat, 11 Dec 2021 15:29:06: 29000000 INFO @ Sat, 11 Dec 2021 15:29:11: 35000000 INFO @ Sat, 11 Dec 2021 15:29:14: 32000000 INFO @ Sat, 11 Dec 2021 15:29:15: 30000000 INFO @ Sat, 11 Dec 2021 15:29:21: 36000000 INFO @ Sat, 11 Dec 2021 15:29:24: 31000000 INFO @ Sat, 11 Dec 2021 15:29:24: 33000000 INFO @ Sat, 11 Dec 2021 15:29:30: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:29:30: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:29:30: #1 total tags in treatment: 17659941 INFO @ Sat, 11 Dec 2021 15:29:30: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:29:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:29:30: #1 tags after filtering in treatment: 15301732 INFO @ Sat, 11 Dec 2021 15:29:30: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 15:29:30: #1 finished! INFO @ Sat, 11 Dec 2021 15:29:30: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:29:30: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:29:32: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 15:29:32: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:29:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:29:33: 32000000 INFO @ Sat, 11 Dec 2021 15:29:34: 34000000 INFO @ Sat, 11 Dec 2021 15:29:42: 33000000 INFO @ Sat, 11 Dec 2021 15:29:43: 35000000 INFO @ Sat, 11 Dec 2021 15:29:50: 34000000 INFO @ Sat, 11 Dec 2021 15:29:52: 36000000 INFO @ Sat, 11 Dec 2021 15:30:00: 35000000 INFO @ Sat, 11 Dec 2021 15:30:02: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:30:02: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:30:02: #1 total tags in treatment: 17659941 INFO @ Sat, 11 Dec 2021 15:30:02: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:30:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:30:02: #1 tags after filtering in treatment: 15301732 INFO @ Sat, 11 Dec 2021 15:30:02: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 15:30:02: #1 finished! INFO @ Sat, 11 Dec 2021 15:30:02: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:30:02: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 15:30:03: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 15:30:03: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:30:03: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 15:30:08: 36000000 INFO @ Sat, 11 Dec 2021 15:30:16: #1 tag size is determined as 50 bps INFO @ Sat, 11 Dec 2021 15:30:16: #1 tag size = 50 INFO @ Sat, 11 Dec 2021 15:30:16: #1 total tags in treatment: 17659941 INFO @ Sat, 11 Dec 2021 15:30:16: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 15:30:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 15:30:17: #1 tags after filtering in treatment: 15301732 INFO @ Sat, 11 Dec 2021 15:30:17: #1 Redundant rate of treatment: 0.13 INFO @ Sat, 11 Dec 2021 15:30:17: #1 finished! INFO @ Sat, 11 Dec 2021 15:30:17: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 15:30:17: #2 looking for paired plus/minus strand peaks... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 15:30:18: #2 number of paired peaks: 85 WARNING @ Sat, 11 Dec 2021 15:30:18: Too few paired peaks (85) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 15:30:18: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 2 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10386198/SRX10386198.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling