Job ID = 14167181 SRX = SRX10367657 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 52145088 spots for SRR13990201/SRR13990201.sra Written 52145088 spots for SRR13990201/SRR13990201.sra fastq に変換しました。 bowtie でマッピング中... Your job 14167958 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:05:20 52145088 reads; of these: 52145088 (100.00%) were paired; of these: 13053263 (25.03%) aligned concordantly 0 times 27391074 (52.53%) aligned concordantly exactly 1 time 11700751 (22.44%) aligned concordantly >1 times ---- 13053263 pairs aligned concordantly 0 times; of these: 4363969 (33.43%) aligned discordantly 1 time ---- 8689294 pairs aligned 0 times concordantly or discordantly; of these: 17378588 mates make up the pairs; of these: 10208199 (58.74%) aligned 0 times 1887386 (10.86%) aligned exactly 1 time 5283003 (30.40%) aligned >1 times 90.21% overall alignment rate Time searching: 01:05:20 Overall time: 01:05:20 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 6581337 / 42746607 = 0.1540 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:16:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:16:27: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:16:27: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:16:32: 1000000 INFO @ Fri, 10 Dec 2021 12:16:37: 2000000 INFO @ Fri, 10 Dec 2021 12:16:42: 3000000 INFO @ Fri, 10 Dec 2021 12:16:47: 4000000 INFO @ Fri, 10 Dec 2021 12:16:53: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:16:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:16:57: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:16:57: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:16:58: 6000000 INFO @ Fri, 10 Dec 2021 12:17:03: 1000000 INFO @ Fri, 10 Dec 2021 12:17:04: 7000000 INFO @ Fri, 10 Dec 2021 12:17:09: 2000000 INFO @ Fri, 10 Dec 2021 12:17:10: 8000000 INFO @ Fri, 10 Dec 2021 12:17:15: 3000000 INFO @ Fri, 10 Dec 2021 12:17:16: 9000000 INFO @ Fri, 10 Dec 2021 12:17:21: 4000000 INFO @ Fri, 10 Dec 2021 12:17:22: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 12:17:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 12:17:27: #1 read tag files... INFO @ Fri, 10 Dec 2021 12:17:27: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 12:17:27: 5000000 INFO @ Fri, 10 Dec 2021 12:17:28: 11000000 INFO @ Fri, 10 Dec 2021 12:17:33: 1000000 INFO @ Fri, 10 Dec 2021 12:17:33: 6000000 INFO @ Fri, 10 Dec 2021 12:17:34: 12000000 INFO @ Fri, 10 Dec 2021 12:17:39: 2000000 INFO @ Fri, 10 Dec 2021 12:17:39: 7000000 INFO @ Fri, 10 Dec 2021 12:17:40: 13000000 INFO @ Fri, 10 Dec 2021 12:17:45: 3000000 INFO @ Fri, 10 Dec 2021 12:17:46: 8000000 INFO @ Fri, 10 Dec 2021 12:17:46: 14000000 INFO @ Fri, 10 Dec 2021 12:17:51: 4000000 INFO @ Fri, 10 Dec 2021 12:17:52: 15000000 INFO @ Fri, 10 Dec 2021 12:17:52: 9000000 INFO @ Fri, 10 Dec 2021 12:17:57: 5000000 INFO @ Fri, 10 Dec 2021 12:17:58: 16000000 INFO @ Fri, 10 Dec 2021 12:17:58: 10000000 INFO @ Fri, 10 Dec 2021 12:18:03: 6000000 INFO @ Fri, 10 Dec 2021 12:18:04: 17000000 INFO @ Fri, 10 Dec 2021 12:18:04: 11000000 INFO @ Fri, 10 Dec 2021 12:18:09: 7000000 INFO @ Fri, 10 Dec 2021 12:18:10: 18000000 INFO @ Fri, 10 Dec 2021 12:18:10: 12000000 INFO @ Fri, 10 Dec 2021 12:18:15: 8000000 INFO @ Fri, 10 Dec 2021 12:18:16: 19000000 INFO @ Fri, 10 Dec 2021 12:18:16: 13000000 INFO @ Fri, 10 Dec 2021 12:18:22: 9000000 INFO @ Fri, 10 Dec 2021 12:18:22: 20000000 INFO @ Fri, 10 Dec 2021 12:18:22: 14000000 INFO @ Fri, 10 Dec 2021 12:18:28: 10000000 INFO @ Fri, 10 Dec 2021 12:18:28: 21000000 INFO @ Fri, 10 Dec 2021 12:18:28: 15000000 INFO @ Fri, 10 Dec 2021 12:18:34: 11000000 INFO @ Fri, 10 Dec 2021 12:18:34: 22000000 INFO @ Fri, 10 Dec 2021 12:18:34: 16000000 INFO @ Fri, 10 Dec 2021 12:18:40: 12000000 INFO @ Fri, 10 Dec 2021 12:18:40: 23000000 INFO @ Fri, 10 Dec 2021 12:18:40: 17000000 INFO @ Fri, 10 Dec 2021 12:18:46: 13000000 INFO @ Fri, 10 Dec 2021 12:18:46: 24000000 INFO @ Fri, 10 Dec 2021 12:18:47: 18000000 INFO @ Fri, 10 Dec 2021 12:18:52: 14000000 INFO @ Fri, 10 Dec 2021 12:18:53: 25000000 INFO @ Fri, 10 Dec 2021 12:18:53: 19000000 INFO @ Fri, 10 Dec 2021 12:18:58: 15000000 INFO @ Fri, 10 Dec 2021 12:18:59: 26000000 INFO @ Fri, 10 Dec 2021 12:18:59: 20000000 INFO @ Fri, 10 Dec 2021 12:19:05: 16000000 INFO @ Fri, 10 Dec 2021 12:19:05: 27000000 INFO @ Fri, 10 Dec 2021 12:19:06: 21000000 INFO @ Fri, 10 Dec 2021 12:19:12: 17000000 INFO @ Fri, 10 Dec 2021 12:19:12: 28000000 INFO @ Fri, 10 Dec 2021 12:19:12: 22000000 INFO @ Fri, 10 Dec 2021 12:19:18: 18000000 INFO @ Fri, 10 Dec 2021 12:19:19: 29000000 INFO @ Fri, 10 Dec 2021 12:19:19: 23000000 INFO @ Fri, 10 Dec 2021 12:19:25: 19000000 INFO @ Fri, 10 Dec 2021 12:19:25: 30000000 INFO @ Fri, 10 Dec 2021 12:19:26: 24000000 INFO @ Fri, 10 Dec 2021 12:19:32: 20000000 INFO @ Fri, 10 Dec 2021 12:19:32: 31000000 INFO @ Fri, 10 Dec 2021 12:19:33: 25000000 INFO @ Fri, 10 Dec 2021 12:19:38: 21000000 INFO @ Fri, 10 Dec 2021 12:19:39: 32000000 INFO @ Fri, 10 Dec 2021 12:19:39: 26000000 INFO @ Fri, 10 Dec 2021 12:19:45: 22000000 INFO @ Fri, 10 Dec 2021 12:19:45: 33000000 INFO @ Fri, 10 Dec 2021 12:19:46: 27000000 INFO @ Fri, 10 Dec 2021 12:19:52: 34000000 INFO @ Fri, 10 Dec 2021 12:19:52: 23000000 INFO @ Fri, 10 Dec 2021 12:19:52: 28000000 INFO @ Fri, 10 Dec 2021 12:19:58: 35000000 INFO @ Fri, 10 Dec 2021 12:19:58: 24000000 INFO @ Fri, 10 Dec 2021 12:19:59: 29000000 INFO @ Fri, 10 Dec 2021 12:20:05: 36000000 INFO @ Fri, 10 Dec 2021 12:20:05: 25000000 INFO @ Fri, 10 Dec 2021 12:20:06: 30000000 INFO @ Fri, 10 Dec 2021 12:20:11: 37000000 INFO @ Fri, 10 Dec 2021 12:20:12: 26000000 INFO @ Fri, 10 Dec 2021 12:20:12: 31000000 INFO @ Fri, 10 Dec 2021 12:20:18: 38000000 INFO @ Fri, 10 Dec 2021 12:20:18: 27000000 INFO @ Fri, 10 Dec 2021 12:20:18: 32000000 INFO @ Fri, 10 Dec 2021 12:20:24: 39000000 INFO @ Fri, 10 Dec 2021 12:20:24: 28000000 INFO @ Fri, 10 Dec 2021 12:20:25: 33000000 INFO @ Fri, 10 Dec 2021 12:20:30: 40000000 INFO @ Fri, 10 Dec 2021 12:20:31: 29000000 INFO @ Fri, 10 Dec 2021 12:20:31: 34000000 INFO @ Fri, 10 Dec 2021 12:20:37: 41000000 INFO @ Fri, 10 Dec 2021 12:20:38: 30000000 INFO @ Fri, 10 Dec 2021 12:20:38: 35000000 INFO @ Fri, 10 Dec 2021 12:20:44: 42000000 INFO @ Fri, 10 Dec 2021 12:20:45: 31000000 INFO @ Fri, 10 Dec 2021 12:20:45: 36000000 INFO @ Fri, 10 Dec 2021 12:20:51: 43000000 INFO @ Fri, 10 Dec 2021 12:20:52: 37000000 INFO @ Fri, 10 Dec 2021 12:20:52: 32000000 INFO @ Fri, 10 Dec 2021 12:20:57: 44000000 INFO @ Fri, 10 Dec 2021 12:20:59: 38000000 INFO @ Fri, 10 Dec 2021 12:21:00: 33000000 INFO @ Fri, 10 Dec 2021 12:21:04: 45000000 INFO @ Fri, 10 Dec 2021 12:21:06: 39000000 INFO @ Fri, 10 Dec 2021 12:21:07: 34000000 INFO @ Fri, 10 Dec 2021 12:21:11: 46000000 INFO @ Fri, 10 Dec 2021 12:21:13: 40000000 INFO @ Fri, 10 Dec 2021 12:21:14: 35000000 INFO @ Fri, 10 Dec 2021 12:21:18: 47000000 INFO @ Fri, 10 Dec 2021 12:21:20: 41000000 INFO @ Fri, 10 Dec 2021 12:21:22: 36000000 INFO @ Fri, 10 Dec 2021 12:21:24: 48000000 INFO @ Fri, 10 Dec 2021 12:21:27: 42000000 INFO @ Fri, 10 Dec 2021 12:21:29: 37000000 INFO @ Fri, 10 Dec 2021 12:21:31: 49000000 INFO @ Fri, 10 Dec 2021 12:21:34: 43000000 INFO @ Fri, 10 Dec 2021 12:21:36: 38000000 INFO @ Fri, 10 Dec 2021 12:21:38: 50000000 INFO @ Fri, 10 Dec 2021 12:21:41: 44000000 INFO @ Fri, 10 Dec 2021 12:21:44: 39000000 INFO @ Fri, 10 Dec 2021 12:21:45: 51000000 INFO @ Fri, 10 Dec 2021 12:21:48: 45000000 INFO @ Fri, 10 Dec 2021 12:21:51: 40000000 INFO @ Fri, 10 Dec 2021 12:21:52: 52000000 INFO @ Fri, 10 Dec 2021 12:21:55: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 12:21:58: 41000000 INFO @ Fri, 10 Dec 2021 12:21:59: 53000000 INFO @ Fri, 10 Dec 2021 12:22:02: 47000000 INFO @ Fri, 10 Dec 2021 12:22:05: 54000000 INFO @ Fri, 10 Dec 2021 12:22:06: 42000000 INFO @ Fri, 10 Dec 2021 12:22:09: 48000000 INFO @ Fri, 10 Dec 2021 12:22:12: 55000000 INFO @ Fri, 10 Dec 2021 12:22:13: 43000000 INFO @ Fri, 10 Dec 2021 12:22:15: 49000000 INFO @ Fri, 10 Dec 2021 12:22:19: 56000000 INFO @ Fri, 10 Dec 2021 12:22:20: 44000000 INFO @ Fri, 10 Dec 2021 12:22:22: 50000000 INFO @ Fri, 10 Dec 2021 12:22:25: 57000000 INFO @ Fri, 10 Dec 2021 12:22:28: 45000000 INFO @ Fri, 10 Dec 2021 12:22:29: 51000000 INFO @ Fri, 10 Dec 2021 12:22:31: 58000000 INFO @ Fri, 10 Dec 2021 12:22:35: 46000000 INFO @ Fri, 10 Dec 2021 12:22:35: 52000000 INFO @ Fri, 10 Dec 2021 12:22:38: 59000000 INFO @ Fri, 10 Dec 2021 12:22:42: 53000000 INFO @ Fri, 10 Dec 2021 12:22:42: 47000000 INFO @ Fri, 10 Dec 2021 12:22:44: 60000000 INFO @ Fri, 10 Dec 2021 12:22:48: 54000000 INFO @ Fri, 10 Dec 2021 12:22:50: 48000000 INFO @ Fri, 10 Dec 2021 12:22:51: 61000000 INFO @ Fri, 10 Dec 2021 12:22:55: 55000000 INFO @ Fri, 10 Dec 2021 12:22:57: 49000000 INFO @ Fri, 10 Dec 2021 12:22:58: 62000000 INFO @ Fri, 10 Dec 2021 12:23:02: 56000000 INFO @ Fri, 10 Dec 2021 12:23:04: 63000000 INFO @ Fri, 10 Dec 2021 12:23:04: 50000000 INFO @ Fri, 10 Dec 2021 12:23:08: 57000000 INFO @ Fri, 10 Dec 2021 12:23:11: 64000000 INFO @ Fri, 10 Dec 2021 12:23:12: 51000000 INFO @ Fri, 10 Dec 2021 12:23:14: 58000000 INFO @ Fri, 10 Dec 2021 12:23:17: 65000000 INFO @ Fri, 10 Dec 2021 12:23:19: 52000000 INFO @ Fri, 10 Dec 2021 12:23:21: 59000000 INFO @ Fri, 10 Dec 2021 12:23:24: 66000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 12:23:26: 53000000 INFO @ Fri, 10 Dec 2021 12:23:27: 60000000 INFO @ Fri, 10 Dec 2021 12:23:31: 67000000 INFO @ Fri, 10 Dec 2021 12:23:34: 61000000 INFO @ Fri, 10 Dec 2021 12:23:34: 54000000 INFO @ Fri, 10 Dec 2021 12:23:37: 68000000 INFO @ Fri, 10 Dec 2021 12:23:40: 62000000 INFO @ Fri, 10 Dec 2021 12:23:41: 55000000 INFO @ Fri, 10 Dec 2021 12:23:44: 69000000 INFO @ Fri, 10 Dec 2021 12:23:47: 63000000 INFO @ Fri, 10 Dec 2021 12:23:49: 56000000 INFO @ Fri, 10 Dec 2021 12:23:51: 70000000 INFO @ Fri, 10 Dec 2021 12:23:54: 64000000 INFO @ Fri, 10 Dec 2021 12:23:56: 57000000 INFO @ Fri, 10 Dec 2021 12:23:58: 71000000 INFO @ Fri, 10 Dec 2021 12:24:01: 65000000 INFO @ Fri, 10 Dec 2021 12:24:03: 58000000 INFO @ Fri, 10 Dec 2021 12:24:04: 72000000 INFO @ Fri, 10 Dec 2021 12:24:08: 66000000 INFO @ Fri, 10 Dec 2021 12:24:10: 59000000 INFO @ Fri, 10 Dec 2021 12:24:11: 73000000 INFO @ Fri, 10 Dec 2021 12:24:15: 67000000 INFO @ Fri, 10 Dec 2021 12:24:17: 60000000 INFO @ Fri, 10 Dec 2021 12:24:18: 74000000 INFO @ Fri, 10 Dec 2021 12:24:22: 68000000 INFO @ Fri, 10 Dec 2021 12:24:25: 61000000 INFO @ Fri, 10 Dec 2021 12:24:25: 75000000 INFO @ Fri, 10 Dec 2021 12:24:29: 69000000 INFO @ Fri, 10 Dec 2021 12:24:32: 76000000 INFO @ Fri, 10 Dec 2021 12:24:32: 62000000 INFO @ Fri, 10 Dec 2021 12:24:35: 70000000 INFO @ Fri, 10 Dec 2021 12:24:38: 77000000 INFO @ Fri, 10 Dec 2021 12:24:40: 63000000 INFO @ Fri, 10 Dec 2021 12:24:42: 71000000 INFO @ Fri, 10 Dec 2021 12:24:45: 78000000 INFO @ Fri, 10 Dec 2021 12:24:47: 64000000 INFO @ Fri, 10 Dec 2021 12:24:49: 72000000 INFO @ Fri, 10 Dec 2021 12:24:52: 79000000 INFO @ Fri, 10 Dec 2021 12:24:55: 65000000 INFO @ Fri, 10 Dec 2021 12:24:56: 73000000 INFO @ Fri, 10 Dec 2021 12:24:59: 80000000 INFO @ Fri, 10 Dec 2021 12:25:02: 66000000 INFO @ Fri, 10 Dec 2021 12:25:02: 74000000 INFO @ Fri, 10 Dec 2021 12:25:05: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 12:25:05: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 12:25:05: #1 total tags in treatment: 33028398 INFO @ Fri, 10 Dec 2021 12:25:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:25:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:25:06: #1 tags after filtering in treatment: 22543079 INFO @ Fri, 10 Dec 2021 12:25:06: #1 Redundant rate of treatment: 0.32 INFO @ Fri, 10 Dec 2021 12:25:06: #1 finished! INFO @ Fri, 10 Dec 2021 12:25:06: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:25:06: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:25:07: #2 number of paired peaks: 214 WARNING @ Fri, 10 Dec 2021 12:25:07: Fewer paired peaks (214) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 214 pairs to build model! INFO @ Fri, 10 Dec 2021 12:25:07: start model_add_line... INFO @ Fri, 10 Dec 2021 12:25:07: start X-correlation... INFO @ Fri, 10 Dec 2021 12:25:07: end of X-cor INFO @ Fri, 10 Dec 2021 12:25:07: #2 finished! INFO @ Fri, 10 Dec 2021 12:25:07: #2 predicted fragment length is 56 bps INFO @ Fri, 10 Dec 2021 12:25:07: #2 alternative fragment length(s) may be 3,56 bps INFO @ Fri, 10 Dec 2021 12:25:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.05_model.r WARNING @ Fri, 10 Dec 2021 12:25:08: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:25:08: #2 You may need to consider one of the other alternative d(s): 3,56 WARNING @ Fri, 10 Dec 2021 12:25:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:25:08: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:25:08: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:25:09: 75000000 INFO @ Fri, 10 Dec 2021 12:25:09: 67000000 INFO @ Fri, 10 Dec 2021 12:25:15: 76000000 INFO @ Fri, 10 Dec 2021 12:25:16: 68000000 INFO @ Fri, 10 Dec 2021 12:25:22: 77000000 INFO @ Fri, 10 Dec 2021 12:25:23: 69000000 INFO @ Fri, 10 Dec 2021 12:25:28: 78000000 INFO @ Fri, 10 Dec 2021 12:25:31: 70000000 INFO @ Fri, 10 Dec 2021 12:25:34: 79000000 INFO @ Fri, 10 Dec 2021 12:25:38: 71000000 INFO @ Fri, 10 Dec 2021 12:25:41: 80000000 INFO @ Fri, 10 Dec 2021 12:25:45: 72000000 INFO @ Fri, 10 Dec 2021 12:25:47: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 12:25:47: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 12:25:47: #1 total tags in treatment: 33028398 INFO @ Fri, 10 Dec 2021 12:25:47: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:25:47: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:25:47: #1 tags after filtering in treatment: 22543079 INFO @ Fri, 10 Dec 2021 12:25:47: #1 Redundant rate of treatment: 0.32 INFO @ Fri, 10 Dec 2021 12:25:47: #1 finished! INFO @ Fri, 10 Dec 2021 12:25:47: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:25:47: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:25:47: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:25:49: #2 number of paired peaks: 214 WARNING @ Fri, 10 Dec 2021 12:25:49: Fewer paired peaks (214) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 214 pairs to build model! INFO @ Fri, 10 Dec 2021 12:25:49: start model_add_line... INFO @ Fri, 10 Dec 2021 12:25:49: start X-correlation... INFO @ Fri, 10 Dec 2021 12:25:49: end of X-cor INFO @ Fri, 10 Dec 2021 12:25:49: #2 finished! INFO @ Fri, 10 Dec 2021 12:25:49: #2 predicted fragment length is 56 bps INFO @ Fri, 10 Dec 2021 12:25:49: #2 alternative fragment length(s) may be 3,56 bps INFO @ Fri, 10 Dec 2021 12:25:49: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.10_model.r WARNING @ Fri, 10 Dec 2021 12:25:49: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:25:49: #2 You may need to consider one of the other alternative d(s): 3,56 WARNING @ Fri, 10 Dec 2021 12:25:49: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:25:49: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:25:49: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:25:52: 73000000 INFO @ Fri, 10 Dec 2021 12:25:59: 74000000 INFO @ Fri, 10 Dec 2021 12:26:05: 75000000 INFO @ Fri, 10 Dec 2021 12:26:07: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.05_peaks.xls INFO @ Fri, 10 Dec 2021 12:26:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:26:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.05_summits.bed INFO @ Fri, 10 Dec 2021 12:26:08: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (16991 records, 4 fields): 16 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:26:12: 76000000 INFO @ Fri, 10 Dec 2021 12:26:19: 77000000 INFO @ Fri, 10 Dec 2021 12:26:25: 78000000 INFO @ Fri, 10 Dec 2021 12:26:28: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:26:31: 79000000 INFO @ Fri, 10 Dec 2021 12:26:38: 80000000 INFO @ Fri, 10 Dec 2021 12:26:44: #1 tag size is determined as 51 bps INFO @ Fri, 10 Dec 2021 12:26:44: #1 tag size = 51 INFO @ Fri, 10 Dec 2021 12:26:44: #1 total tags in treatment: 33028398 INFO @ Fri, 10 Dec 2021 12:26:44: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 12:26:44: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 12:26:45: #1 tags after filtering in treatment: 22543079 INFO @ Fri, 10 Dec 2021 12:26:45: #1 Redundant rate of treatment: 0.32 INFO @ Fri, 10 Dec 2021 12:26:45: #1 finished! INFO @ Fri, 10 Dec 2021 12:26:45: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 12:26:45: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 12:26:46: #2 number of paired peaks: 214 WARNING @ Fri, 10 Dec 2021 12:26:46: Fewer paired peaks (214) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 214 pairs to build model! INFO @ Fri, 10 Dec 2021 12:26:46: start model_add_line... INFO @ Fri, 10 Dec 2021 12:26:46: start X-correlation... INFO @ Fri, 10 Dec 2021 12:26:46: end of X-cor INFO @ Fri, 10 Dec 2021 12:26:46: #2 finished! INFO @ Fri, 10 Dec 2021 12:26:46: #2 predicted fragment length is 56 bps INFO @ Fri, 10 Dec 2021 12:26:46: #2 alternative fragment length(s) may be 3,56 bps INFO @ Fri, 10 Dec 2021 12:26:46: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.20_model.r WARNING @ Fri, 10 Dec 2021 12:26:46: #2 Since the d (56) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 12:26:46: #2 You may need to consider one of the other alternative d(s): 3,56 WARNING @ Fri, 10 Dec 2021 12:26:46: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 12:26:46: #3 Call peaks... INFO @ Fri, 10 Dec 2021 12:26:46: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 12:26:47: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.10_peaks.xls INFO @ Fri, 10 Dec 2021 12:26:47: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:26:48: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.10_summits.bed INFO @ Fri, 10 Dec 2021 12:26:48: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (8412 records, 4 fields): 42 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 12:27:23: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 12:27:41: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.20_peaks.xls INFO @ Fri, 10 Dec 2021 12:27:42: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 12:27:42: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10367657/SRX10367657.20_summits.bed INFO @ Fri, 10 Dec 2021 12:27:42: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1572 records, 4 fields): 100 millis CompletedMACS2peakCalling