Job ID = 6527524 SRX = SRX101480 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:58:54 prefetch.2.10.7: 1) Downloading 'SRR353684'... 2020-06-29T12:58:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:01:29 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:01:29 prefetch.2.10.7: 1) 'SRR353684' was downloaded successfully Read 33954493 spots for SRR353684/SRR353684.sra Written 33954493 spots for SRR353684/SRR353684.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:53 33954493 reads; of these: 33954493 (100.00%) were unpaired; of these: 5300554 (15.61%) aligned 0 times 22578203 (66.50%) aligned exactly 1 time 6075736 (17.89%) aligned >1 times 84.39% overall alignment rate Time searching: 00:08:53 Overall time: 00:08:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 5532430 / 28653939 = 0.1931 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:24:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:24:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:24:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:24:52: 1000000 INFO @ Mon, 29 Jun 2020 22:24:58: 2000000 INFO @ Mon, 29 Jun 2020 22:25:03: 3000000 INFO @ Mon, 29 Jun 2020 22:25:08: 4000000 INFO @ Mon, 29 Jun 2020 22:25:13: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:25:17: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:25:17: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:25:17: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:25:19: 6000000 INFO @ Mon, 29 Jun 2020 22:25:23: 1000000 INFO @ Mon, 29 Jun 2020 22:25:24: 7000000 INFO @ Mon, 29 Jun 2020 22:25:28: 2000000 INFO @ Mon, 29 Jun 2020 22:25:30: 8000000 INFO @ Mon, 29 Jun 2020 22:25:34: 3000000 INFO @ Mon, 29 Jun 2020 22:25:35: 9000000 INFO @ Mon, 29 Jun 2020 22:25:39: 4000000 INFO @ Mon, 29 Jun 2020 22:25:40: 10000000 INFO @ Mon, 29 Jun 2020 22:25:45: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:25:46: 11000000 INFO @ Mon, 29 Jun 2020 22:25:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:25:47: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:25:47: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:25:50: 6000000 INFO @ Mon, 29 Jun 2020 22:25:51: 12000000 INFO @ Mon, 29 Jun 2020 22:25:53: 1000000 INFO @ Mon, 29 Jun 2020 22:25:56: 7000000 INFO @ Mon, 29 Jun 2020 22:25:57: 13000000 INFO @ Mon, 29 Jun 2020 22:25:58: 2000000 INFO @ Mon, 29 Jun 2020 22:26:01: 8000000 INFO @ Mon, 29 Jun 2020 22:26:02: 14000000 INFO @ Mon, 29 Jun 2020 22:26:04: 3000000 INFO @ Mon, 29 Jun 2020 22:26:07: 9000000 INFO @ Mon, 29 Jun 2020 22:26:08: 15000000 INFO @ Mon, 29 Jun 2020 22:26:09: 4000000 INFO @ Mon, 29 Jun 2020 22:26:12: 10000000 INFO @ Mon, 29 Jun 2020 22:26:13: 16000000 INFO @ Mon, 29 Jun 2020 22:26:15: 5000000 INFO @ Mon, 29 Jun 2020 22:26:18: 11000000 INFO @ Mon, 29 Jun 2020 22:26:18: 17000000 INFO @ Mon, 29 Jun 2020 22:26:20: 6000000 INFO @ Mon, 29 Jun 2020 22:26:23: 12000000 INFO @ Mon, 29 Jun 2020 22:26:24: 18000000 INFO @ Mon, 29 Jun 2020 22:26:25: 7000000 INFO @ Mon, 29 Jun 2020 22:26:29: 13000000 INFO @ Mon, 29 Jun 2020 22:26:29: 19000000 INFO @ Mon, 29 Jun 2020 22:26:31: 8000000 INFO @ Mon, 29 Jun 2020 22:26:34: 14000000 INFO @ Mon, 29 Jun 2020 22:26:35: 20000000 INFO @ Mon, 29 Jun 2020 22:26:36: 9000000 INFO @ Mon, 29 Jun 2020 22:26:40: 15000000 INFO @ Mon, 29 Jun 2020 22:26:40: 21000000 INFO @ Mon, 29 Jun 2020 22:26:42: 10000000 INFO @ Mon, 29 Jun 2020 22:26:45: 16000000 INFO @ Mon, 29 Jun 2020 22:26:46: 22000000 INFO @ Mon, 29 Jun 2020 22:26:48: 11000000 INFO @ Mon, 29 Jun 2020 22:26:51: 17000000 INFO @ Mon, 29 Jun 2020 22:26:51: 23000000 INFO @ Mon, 29 Jun 2020 22:26:52: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 22:26:52: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 22:26:52: #1 total tags in treatment: 23121509 INFO @ Mon, 29 Jun 2020 22:26:52: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:26:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:26:52: #1 tags after filtering in treatment: 23121509 INFO @ Mon, 29 Jun 2020 22:26:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:26:52: #1 finished! INFO @ Mon, 29 Jun 2020 22:26:52: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:26:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:26:53: 12000000 INFO @ Mon, 29 Jun 2020 22:26:54: #2 number of paired peaks: 18 WARNING @ Mon, 29 Jun 2020 22:26:54: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:26:54: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:26:56: 18000000 INFO @ Mon, 29 Jun 2020 22:26:58: 13000000 INFO @ Mon, 29 Jun 2020 22:27:02: 19000000 INFO @ Mon, 29 Jun 2020 22:27:04: 14000000 INFO @ Mon, 29 Jun 2020 22:27:07: 20000000 INFO @ Mon, 29 Jun 2020 22:27:09: 15000000 INFO @ Mon, 29 Jun 2020 22:27:12: 21000000 INFO @ Mon, 29 Jun 2020 22:27:14: 16000000 INFO @ Mon, 29 Jun 2020 22:27:18: 22000000 INFO @ Mon, 29 Jun 2020 22:27:20: 17000000 INFO @ Mon, 29 Jun 2020 22:27:23: 23000000 INFO @ Mon, 29 Jun 2020 22:27:24: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 22:27:24: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 22:27:24: #1 total tags in treatment: 23121509 INFO @ Mon, 29 Jun 2020 22:27:24: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:27:24: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:27:24: #1 tags after filtering in treatment: 23121509 INFO @ Mon, 29 Jun 2020 22:27:24: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:27:24: #1 finished! INFO @ Mon, 29 Jun 2020 22:27:24: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:27:24: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:27:25: 18000000 INFO @ Mon, 29 Jun 2020 22:27:26: #2 number of paired peaks: 18 WARNING @ Mon, 29 Jun 2020 22:27:26: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:27:26: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:27:30: 19000000 INFO @ Mon, 29 Jun 2020 22:27:35: 20000000 INFO @ Mon, 29 Jun 2020 22:27:40: 21000000 INFO @ Mon, 29 Jun 2020 22:27:46: 22000000 INFO @ Mon, 29 Jun 2020 22:27:51: 23000000 INFO @ Mon, 29 Jun 2020 22:27:51: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 22:27:51: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 22:27:51: #1 total tags in treatment: 23121509 INFO @ Mon, 29 Jun 2020 22:27:51: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:27:51: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:27:52: #1 tags after filtering in treatment: 23121509 INFO @ Mon, 29 Jun 2020 22:27:52: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:27:52: #1 finished! INFO @ Mon, 29 Jun 2020 22:27:52: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:27:52: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:27:53: #2 number of paired peaks: 18 WARNING @ Mon, 29 Jun 2020 22:27:53: Too few paired peaks (18) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:27:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101480/SRX101480.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。