Job ID = 6527523 SRX = SRX101477 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:47:30 prefetch.2.10.7: 1) Downloading 'SRR353681'... 2020-06-29T13:47:30 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:50:44 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:50:44 prefetch.2.10.7: 1) 'SRR353681' was downloaded successfully Read 37152226 spots for SRR353681/SRR353681.sra Written 37152226 spots for SRR353681/SRR353681.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:09:47 37152226 reads; of these: 37152226 (100.00%) were unpaired; of these: 14055198 (37.83%) aligned 0 times 17318274 (46.61%) aligned exactly 1 time 5778754 (15.55%) aligned >1 times 62.17% overall alignment rate Time searching: 00:09:47 Overall time: 00:09:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3018435 / 23097028 = 0.1307 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:17:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:17:15: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:17:15: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:17:21: 1000000 INFO @ Mon, 29 Jun 2020 23:17:27: 2000000 INFO @ Mon, 29 Jun 2020 23:17:33: 3000000 INFO @ Mon, 29 Jun 2020 23:17:39: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:17:45: 5000000 INFO @ Mon, 29 Jun 2020 23:17:45: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:17:45: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:17:45: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:17:51: 6000000 INFO @ Mon, 29 Jun 2020 23:17:52: 1000000 INFO @ Mon, 29 Jun 2020 23:17:57: 7000000 INFO @ Mon, 29 Jun 2020 23:17:58: 2000000 INFO @ Mon, 29 Jun 2020 23:18:03: 8000000 INFO @ Mon, 29 Jun 2020 23:18:05: 3000000 INFO @ Mon, 29 Jun 2020 23:18:09: 9000000 INFO @ Mon, 29 Jun 2020 23:18:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 23:18:15: 10000000 INFO @ Mon, 29 Jun 2020 23:18:15: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 23:18:15: #1 read tag files... INFO @ Mon, 29 Jun 2020 23:18:15: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 23:18:18: 5000000 INFO @ Mon, 29 Jun 2020 23:18:22: 11000000 INFO @ Mon, 29 Jun 2020 23:18:23: 1000000 INFO @ Mon, 29 Jun 2020 23:18:25: 6000000 INFO @ Mon, 29 Jun 2020 23:18:28: 12000000 INFO @ Mon, 29 Jun 2020 23:18:30: 2000000 INFO @ Mon, 29 Jun 2020 23:18:31: 7000000 INFO @ Mon, 29 Jun 2020 23:18:35: 13000000 INFO @ Mon, 29 Jun 2020 23:18:37: 3000000 INFO @ Mon, 29 Jun 2020 23:18:38: 8000000 INFO @ Mon, 29 Jun 2020 23:18:42: 14000000 INFO @ Mon, 29 Jun 2020 23:18:44: 4000000 INFO @ Mon, 29 Jun 2020 23:18:44: 9000000 INFO @ Mon, 29 Jun 2020 23:18:49: 15000000 INFO @ Mon, 29 Jun 2020 23:18:51: 10000000 INFO @ Mon, 29 Jun 2020 23:18:51: 5000000 INFO @ Mon, 29 Jun 2020 23:18:56: 16000000 INFO @ Mon, 29 Jun 2020 23:18:57: 11000000 INFO @ Mon, 29 Jun 2020 23:18:58: 6000000 INFO @ Mon, 29 Jun 2020 23:19:03: 17000000 INFO @ Mon, 29 Jun 2020 23:19:04: 12000000 INFO @ Mon, 29 Jun 2020 23:19:05: 7000000 INFO @ Mon, 29 Jun 2020 23:19:10: 13000000 INFO @ Mon, 29 Jun 2020 23:19:11: 18000000 INFO @ Mon, 29 Jun 2020 23:19:13: 8000000 INFO @ Mon, 29 Jun 2020 23:19:17: 14000000 INFO @ Mon, 29 Jun 2020 23:19:17: 19000000 INFO @ Mon, 29 Jun 2020 23:19:20: 9000000 INFO @ Mon, 29 Jun 2020 23:19:23: 15000000 INFO @ Mon, 29 Jun 2020 23:19:25: 20000000 INFO @ Mon, 29 Jun 2020 23:19:26: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 23:19:26: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 23:19:26: #1 total tags in treatment: 20078593 INFO @ Mon, 29 Jun 2020 23:19:26: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:19:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:19:26: #1 tags after filtering in treatment: 20078593 INFO @ Mon, 29 Jun 2020 23:19:26: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:19:26: #1 finished! INFO @ Mon, 29 Jun 2020 23:19:26: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:19:26: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:19:27: 10000000 INFO @ Mon, 29 Jun 2020 23:19:27: #2 number of paired peaks: 74 WARNING @ Mon, 29 Jun 2020 23:19:27: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:19:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:19:30: 16000000 INFO @ Mon, 29 Jun 2020 23:19:33: 11000000 INFO @ Mon, 29 Jun 2020 23:19:37: 17000000 INFO @ Mon, 29 Jun 2020 23:19:40: 12000000 INFO @ Mon, 29 Jun 2020 23:19:43: 18000000 INFO @ Mon, 29 Jun 2020 23:19:46: 13000000 INFO @ Mon, 29 Jun 2020 23:19:50: 19000000 INFO @ Mon, 29 Jun 2020 23:19:53: 14000000 INFO @ Mon, 29 Jun 2020 23:19:56: 20000000 INFO @ Mon, 29 Jun 2020 23:19:57: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 23:19:57: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 23:19:57: #1 total tags in treatment: 20078593 INFO @ Mon, 29 Jun 2020 23:19:57: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:19:57: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:19:57: #1 tags after filtering in treatment: 20078593 INFO @ Mon, 29 Jun 2020 23:19:57: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:19:57: #1 finished! INFO @ Mon, 29 Jun 2020 23:19:57: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:19:57: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:19:58: #2 number of paired peaks: 74 WARNING @ Mon, 29 Jun 2020 23:19:58: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:19:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 23:19:59: 15000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 23:20:06: 16000000 INFO @ Mon, 29 Jun 2020 23:20:12: 17000000 INFO @ Mon, 29 Jun 2020 23:20:18: 18000000 INFO @ Mon, 29 Jun 2020 23:20:24: 19000000 INFO @ Mon, 29 Jun 2020 23:20:31: 20000000 INFO @ Mon, 29 Jun 2020 23:20:31: #1 tag size is determined as 41 bps INFO @ Mon, 29 Jun 2020 23:20:31: #1 tag size = 41 INFO @ Mon, 29 Jun 2020 23:20:31: #1 total tags in treatment: 20078593 INFO @ Mon, 29 Jun 2020 23:20:31: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 23:20:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 23:20:32: #1 tags after filtering in treatment: 20078593 INFO @ Mon, 29 Jun 2020 23:20:32: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 23:20:32: #1 finished! INFO @ Mon, 29 Jun 2020 23:20:32: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 23:20:32: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 23:20:33: #2 number of paired peaks: 74 WARNING @ Mon, 29 Jun 2020 23:20:33: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 23:20:33: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX101477/SRX101477.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。