Job ID = 14172113 SRX = SRX10089761 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 32853039 spots for SRR13700648/SRR13700648.sra Written 32853039 spots for SRR13700648/SRR13700648.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172601 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:50 32853039 reads; of these: 32853039 (100.00%) were unpaired; of these: 766163 (2.33%) aligned 0 times 23382483 (71.17%) aligned exactly 1 time 8704393 (26.49%) aligned >1 times 97.67% overall alignment rate Time searching: 00:13:50 Overall time: 00:13:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 4752814 / 32086876 = 0.1481 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:41:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:41:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:41:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:41:10: 1000000 INFO @ Sat, 11 Dec 2021 14:41:16: 2000000 INFO @ Sat, 11 Dec 2021 14:41:22: 3000000 INFO @ Sat, 11 Dec 2021 14:41:28: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:41:33: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:41:33: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:41:33: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:41:34: 5000000 INFO @ Sat, 11 Dec 2021 14:41:39: 1000000 INFO @ Sat, 11 Dec 2021 14:41:40: 6000000 INFO @ Sat, 11 Dec 2021 14:41:45: 2000000 INFO @ Sat, 11 Dec 2021 14:41:46: 7000000 INFO @ Sat, 11 Dec 2021 14:41:50: 3000000 INFO @ Sat, 11 Dec 2021 14:41:52: 8000000 INFO @ Sat, 11 Dec 2021 14:41:56: 4000000 INFO @ Sat, 11 Dec 2021 14:41:57: 9000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:42:02: 5000000 INFO @ Sat, 11 Dec 2021 14:42:03: 10000000 INFO @ Sat, 11 Dec 2021 14:42:03: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:42:03: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:42:03: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:42:08: 6000000 INFO @ Sat, 11 Dec 2021 14:42:09: 11000000 INFO @ Sat, 11 Dec 2021 14:42:10: 1000000 INFO @ Sat, 11 Dec 2021 14:42:13: 7000000 INFO @ Sat, 11 Dec 2021 14:42:14: 12000000 INFO @ Sat, 11 Dec 2021 14:42:16: 2000000 INFO @ Sat, 11 Dec 2021 14:42:19: 8000000 INFO @ Sat, 11 Dec 2021 14:42:20: 13000000 INFO @ Sat, 11 Dec 2021 14:42:22: 3000000 INFO @ Sat, 11 Dec 2021 14:42:25: 9000000 INFO @ Sat, 11 Dec 2021 14:42:25: 14000000 INFO @ Sat, 11 Dec 2021 14:42:29: 4000000 INFO @ Sat, 11 Dec 2021 14:42:30: 10000000 INFO @ Sat, 11 Dec 2021 14:42:31: 15000000 INFO @ Sat, 11 Dec 2021 14:42:35: 5000000 INFO @ Sat, 11 Dec 2021 14:42:36: 11000000 INFO @ Sat, 11 Dec 2021 14:42:37: 16000000 INFO @ Sat, 11 Dec 2021 14:42:41: 6000000 INFO @ Sat, 11 Dec 2021 14:42:42: 12000000 INFO @ Sat, 11 Dec 2021 14:42:42: 17000000 INFO @ Sat, 11 Dec 2021 14:42:47: 13000000 INFO @ Sat, 11 Dec 2021 14:42:48: 18000000 INFO @ Sat, 11 Dec 2021 14:42:48: 7000000 INFO @ Sat, 11 Dec 2021 14:42:53: 14000000 INFO @ Sat, 11 Dec 2021 14:42:53: 19000000 INFO @ Sat, 11 Dec 2021 14:42:54: 8000000 INFO @ Sat, 11 Dec 2021 14:42:59: 15000000 INFO @ Sat, 11 Dec 2021 14:42:59: 20000000 INFO @ Sat, 11 Dec 2021 14:43:01: 9000000 INFO @ Sat, 11 Dec 2021 14:43:04: 16000000 INFO @ Sat, 11 Dec 2021 14:43:05: 21000000 INFO @ Sat, 11 Dec 2021 14:43:08: 10000000 INFO @ Sat, 11 Dec 2021 14:43:10: 17000000 INFO @ Sat, 11 Dec 2021 14:43:11: 22000000 INFO @ Sat, 11 Dec 2021 14:43:14: 11000000 INFO @ Sat, 11 Dec 2021 14:43:16: 18000000 INFO @ Sat, 11 Dec 2021 14:43:16: 23000000 INFO @ Sat, 11 Dec 2021 14:43:20: 12000000 INFO @ Sat, 11 Dec 2021 14:43:21: 19000000 INFO @ Sat, 11 Dec 2021 14:43:22: 24000000 INFO @ Sat, 11 Dec 2021 14:43:27: 13000000 INFO @ Sat, 11 Dec 2021 14:43:27: 20000000 INFO @ Sat, 11 Dec 2021 14:43:28: 25000000 INFO @ Sat, 11 Dec 2021 14:43:33: 21000000 INFO @ Sat, 11 Dec 2021 14:43:33: 14000000 INFO @ Sat, 11 Dec 2021 14:43:33: 26000000 INFO @ Sat, 11 Dec 2021 14:43:39: 22000000 INFO @ Sat, 11 Dec 2021 14:43:39: 27000000 INFO @ Sat, 11 Dec 2021 14:43:39: 15000000 INFO @ Sat, 11 Dec 2021 14:43:41: #1 tag size is determined as 74 bps INFO @ Sat, 11 Dec 2021 14:43:41: #1 tag size = 74 INFO @ Sat, 11 Dec 2021 14:43:41: #1 total tags in treatment: 27334062 INFO @ Sat, 11 Dec 2021 14:43:41: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:43:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:43:41: #1 tags after filtering in treatment: 27334062 INFO @ Sat, 11 Dec 2021 14:43:41: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:43:41: #1 finished! INFO @ Sat, 11 Dec 2021 14:43:41: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:43:41: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:43:43: #2 number of paired peaks: 53 WARNING @ Sat, 11 Dec 2021 14:43:43: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:43:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:43:44: 23000000 INFO @ Sat, 11 Dec 2021 14:43:46: 16000000 INFO @ Sat, 11 Dec 2021 14:43:50: 24000000 INFO @ Sat, 11 Dec 2021 14:43:52: 17000000 INFO @ Sat, 11 Dec 2021 14:43:56: 25000000 INFO @ Sat, 11 Dec 2021 14:43:58: 18000000 INFO @ Sat, 11 Dec 2021 14:44:01: 26000000 INFO @ Sat, 11 Dec 2021 14:44:05: 19000000 INFO @ Sat, 11 Dec 2021 14:44:07: 27000000 INFO @ Sat, 11 Dec 2021 14:44:09: #1 tag size is determined as 74 bps INFO @ Sat, 11 Dec 2021 14:44:09: #1 tag size = 74 INFO @ Sat, 11 Dec 2021 14:44:09: #1 total tags in treatment: 27334062 INFO @ Sat, 11 Dec 2021 14:44:09: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:44:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:44:09: #1 tags after filtering in treatment: 27334062 INFO @ Sat, 11 Dec 2021 14:44:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:44:09: #1 finished! INFO @ Sat, 11 Dec 2021 14:44:09: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:44:09: #2 looking for paired plus/minus strand peaks... BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:44:11: 20000000 INFO @ Sat, 11 Dec 2021 14:44:11: #2 number of paired peaks: 53 WARNING @ Sat, 11 Dec 2021 14:44:11: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:44:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:44:17: 21000000 INFO @ Sat, 11 Dec 2021 14:44:24: 22000000 INFO @ Sat, 11 Dec 2021 14:44:30: 23000000 INFO @ Sat, 11 Dec 2021 14:44:36: 24000000 INFO @ Sat, 11 Dec 2021 14:44:42: 25000000 INFO @ Sat, 11 Dec 2021 14:44:48: 26000000 INFO @ Sat, 11 Dec 2021 14:44:53: 27000000 INFO @ Sat, 11 Dec 2021 14:44:56: #1 tag size is determined as 74 bps INFO @ Sat, 11 Dec 2021 14:44:56: #1 tag size = 74 INFO @ Sat, 11 Dec 2021 14:44:56: #1 total tags in treatment: 27334062 INFO @ Sat, 11 Dec 2021 14:44:56: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:44:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:44:56: #1 tags after filtering in treatment: 27334062 INFO @ Sat, 11 Dec 2021 14:44:56: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:44:56: #1 finished! INFO @ Sat, 11 Dec 2021 14:44:56: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:44:56: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:44:58: #2 number of paired peaks: 53 WARNING @ Sat, 11 Dec 2021 14:44:58: Too few paired peaks (53) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:44:58: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089761/SRX10089761.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。