Job ID = 14172051 SRX = SRX10089753 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 25352395 spots for SRR13700640/SRR13700640.sra Written 25352395 spots for SRR13700640/SRR13700640.sra fastq に変換しました。 bowtie でマッピング中... Your job 14172510 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:09 25352395 reads; of these: 25352395 (100.00%) were unpaired; of these: 2319139 (9.15%) aligned 0 times 17628200 (69.53%) aligned exactly 1 time 5405056 (21.32%) aligned >1 times 90.85% overall alignment rate Time searching: 00:06:09 Overall time: 00:06:09 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3266549 / 23033256 = 0.1418 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:04:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:04:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:04:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:04:37: 1000000 INFO @ Sat, 11 Dec 2021 14:04:41: 2000000 INFO @ Sat, 11 Dec 2021 14:04:46: 3000000 INFO @ Sat, 11 Dec 2021 14:04:50: 4000000 INFO @ Sat, 11 Dec 2021 14:04:55: 5000000 INFO @ Sat, 11 Dec 2021 14:04:59: 6000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:05:02: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:05:02: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:05:02: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:05:04: 7000000 INFO @ Sat, 11 Dec 2021 14:05:08: 1000000 INFO @ Sat, 11 Dec 2021 14:05:09: 8000000 INFO @ Sat, 11 Dec 2021 14:05:13: 2000000 INFO @ Sat, 11 Dec 2021 14:05:14: 9000000 INFO @ Sat, 11 Dec 2021 14:05:19: 3000000 INFO @ Sat, 11 Dec 2021 14:05:19: 10000000 INFO @ Sat, 11 Dec 2021 14:05:24: 11000000 INFO @ Sat, 11 Dec 2021 14:05:24: 4000000 INFO @ Sat, 11 Dec 2021 14:05:29: 12000000 INFO @ Sat, 11 Dec 2021 14:05:30: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 14:05:32: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 14:05:32: #1 read tag files... INFO @ Sat, 11 Dec 2021 14:05:32: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 14:05:34: 13000000 INFO @ Sat, 11 Dec 2021 14:05:35: 6000000 INFO @ Sat, 11 Dec 2021 14:05:37: 1000000 INFO @ Sat, 11 Dec 2021 14:05:39: 14000000 INFO @ Sat, 11 Dec 2021 14:05:41: 7000000 INFO @ Sat, 11 Dec 2021 14:05:42: 2000000 INFO @ Sat, 11 Dec 2021 14:05:44: 15000000 INFO @ Sat, 11 Dec 2021 14:05:46: 8000000 INFO @ Sat, 11 Dec 2021 14:05:47: 3000000 INFO @ Sat, 11 Dec 2021 14:05:49: 16000000 INFO @ Sat, 11 Dec 2021 14:05:52: 4000000 INFO @ Sat, 11 Dec 2021 14:05:52: 9000000 INFO @ Sat, 11 Dec 2021 14:05:54: 17000000 INFO @ Sat, 11 Dec 2021 14:05:57: 5000000 INFO @ Sat, 11 Dec 2021 14:05:58: 10000000 INFO @ Sat, 11 Dec 2021 14:05:59: 18000000 INFO @ Sat, 11 Dec 2021 14:06:02: 6000000 INFO @ Sat, 11 Dec 2021 14:06:03: 11000000 INFO @ Sat, 11 Dec 2021 14:06:04: 19000000 INFO @ Sat, 11 Dec 2021 14:06:07: 7000000 INFO @ Sat, 11 Dec 2021 14:06:08: #1 tag size is determined as 41 bps INFO @ Sat, 11 Dec 2021 14:06:08: #1 tag size = 41 INFO @ Sat, 11 Dec 2021 14:06:08: #1 total tags in treatment: 19766707 INFO @ Sat, 11 Dec 2021 14:06:08: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:06:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:06:08: #1 tags after filtering in treatment: 19766707 INFO @ Sat, 11 Dec 2021 14:06:08: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:06:08: #1 finished! INFO @ Sat, 11 Dec 2021 14:06:08: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:06:08: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:06:09: 12000000 INFO @ Sat, 11 Dec 2021 14:06:09: #2 number of paired peaks: 74 WARNING @ Sat, 11 Dec 2021 14:06:09: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:06:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:06:12: 8000000 INFO @ Sat, 11 Dec 2021 14:06:14: 13000000 INFO @ Sat, 11 Dec 2021 14:06:17: 9000000 INFO @ Sat, 11 Dec 2021 14:06:20: 14000000 INFO @ Sat, 11 Dec 2021 14:06:22: 10000000 INFO @ Sat, 11 Dec 2021 14:06:25: 15000000 INFO @ Sat, 11 Dec 2021 14:06:26: 11000000 INFO @ Sat, 11 Dec 2021 14:06:31: 16000000 INFO @ Sat, 11 Dec 2021 14:06:31: 12000000 INFO @ Sat, 11 Dec 2021 14:06:36: 13000000 INFO @ Sat, 11 Dec 2021 14:06:36: 17000000 INFO @ Sat, 11 Dec 2021 14:06:41: 14000000 INFO @ Sat, 11 Dec 2021 14:06:42: 18000000 INFO @ Sat, 11 Dec 2021 14:06:46: 15000000 INFO @ Sat, 11 Dec 2021 14:06:47: 19000000 INFO @ Sat, 11 Dec 2021 14:06:51: 16000000 INFO @ Sat, 11 Dec 2021 14:06:52: #1 tag size is determined as 41 bps INFO @ Sat, 11 Dec 2021 14:06:52: #1 tag size = 41 INFO @ Sat, 11 Dec 2021 14:06:52: #1 total tags in treatment: 19766707 INFO @ Sat, 11 Dec 2021 14:06:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:06:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:06:52: #1 tags after filtering in treatment: 19766707 INFO @ Sat, 11 Dec 2021 14:06:52: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:06:52: #1 finished! INFO @ Sat, 11 Dec 2021 14:06:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:06:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:06:53: #2 number of paired peaks: 74 WARNING @ Sat, 11 Dec 2021 14:06:53: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:06:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 14:06:56: 17000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 14:07:01: 18000000 INFO @ Sat, 11 Dec 2021 14:07:05: 19000000 INFO @ Sat, 11 Dec 2021 14:07:09: #1 tag size is determined as 41 bps INFO @ Sat, 11 Dec 2021 14:07:09: #1 tag size = 41 INFO @ Sat, 11 Dec 2021 14:07:09: #1 total tags in treatment: 19766707 INFO @ Sat, 11 Dec 2021 14:07:09: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 14:07:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 14:07:09: #1 tags after filtering in treatment: 19766707 INFO @ Sat, 11 Dec 2021 14:07:09: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 14:07:09: #1 finished! INFO @ Sat, 11 Dec 2021 14:07:09: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 14:07:09: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 14:07:11: #2 number of paired peaks: 74 WARNING @ Sat, 11 Dec 2021 14:07:11: Too few paired peaks (74) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Sat, 11 Dec 2021 14:07:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10089753/SRX10089753.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。