Job ID = 16438135 SRX = SRX10031711 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 129707306 spots for SRR13634481/SRR13634481.sra Written 129707306 spots for SRR13634481/SRR13634481.sra fastq に変換しました。 bowtie でマッピング中... Your job 16439296 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:59:04 129707306 reads; of these: 129707306 (100.00%) were unpaired; of these: 48887864 (37.69%) aligned 0 times 63771648 (49.17%) aligned exactly 1 time 17047794 (13.14%) aligned >1 times 62.31% overall alignment rate Time searching: 00:59:04 Overall time: 00:59:04 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 36 files... [bam_rmdupse_core] 38640711 / 80819442 = 0.4781 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:09:35: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:09:35: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:09:35: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:09:41: 1000000 INFO @ Tue, 02 Aug 2022 15:09:46: 2000000 INFO @ Tue, 02 Aug 2022 15:09:52: 3000000 INFO @ Tue, 02 Aug 2022 15:09:57: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:10:03: 5000000 INFO @ Tue, 02 Aug 2022 15:10:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:10:04: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:10:04: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:10:09: 6000000 INFO @ Tue, 02 Aug 2022 15:10:10: 1000000 INFO @ Tue, 02 Aug 2022 15:10:14: 7000000 INFO @ Tue, 02 Aug 2022 15:10:16: 2000000 INFO @ Tue, 02 Aug 2022 15:10:20: 8000000 INFO @ Tue, 02 Aug 2022 15:10:22: 3000000 INFO @ Tue, 02 Aug 2022 15:10:26: 9000000 INFO @ Tue, 02 Aug 2022 15:10:27: 4000000 INFO @ Tue, 02 Aug 2022 15:10:31: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 15:10:33: 5000000 INFO @ Tue, 02 Aug 2022 15:10:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 15:10:34: #1 read tag files... INFO @ Tue, 02 Aug 2022 15:10:34: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 15:10:37: 11000000 INFO @ Tue, 02 Aug 2022 15:10:39: 6000000 INFO @ Tue, 02 Aug 2022 15:10:40: 1000000 INFO @ Tue, 02 Aug 2022 15:10:43: 12000000 INFO @ Tue, 02 Aug 2022 15:10:45: 7000000 INFO @ Tue, 02 Aug 2022 15:10:47: 2000000 INFO @ Tue, 02 Aug 2022 15:10:50: 13000000 INFO @ Tue, 02 Aug 2022 15:10:52: 8000000 INFO @ Tue, 02 Aug 2022 15:10:54: 3000000 INFO @ Tue, 02 Aug 2022 15:10:56: 14000000 INFO @ Tue, 02 Aug 2022 15:10:58: 9000000 INFO @ Tue, 02 Aug 2022 15:11:01: 4000000 INFO @ Tue, 02 Aug 2022 15:11:02: 15000000 INFO @ Tue, 02 Aug 2022 15:11:04: 10000000 INFO @ Tue, 02 Aug 2022 15:11:08: 5000000 INFO @ Tue, 02 Aug 2022 15:11:09: 16000000 INFO @ Tue, 02 Aug 2022 15:11:10: 11000000 INFO @ Tue, 02 Aug 2022 15:11:14: 6000000 INFO @ Tue, 02 Aug 2022 15:11:15: 17000000 INFO @ Tue, 02 Aug 2022 15:11:16: 12000000 INFO @ Tue, 02 Aug 2022 15:11:21: 18000000 INFO @ Tue, 02 Aug 2022 15:11:21: 7000000 INFO @ Tue, 02 Aug 2022 15:11:23: 13000000 INFO @ Tue, 02 Aug 2022 15:11:27: 19000000 INFO @ Tue, 02 Aug 2022 15:11:28: 8000000 INFO @ Tue, 02 Aug 2022 15:11:29: 14000000 INFO @ Tue, 02 Aug 2022 15:11:33: 20000000 INFO @ Tue, 02 Aug 2022 15:11:34: 9000000 INFO @ Tue, 02 Aug 2022 15:11:35: 15000000 INFO @ Tue, 02 Aug 2022 15:11:39: 21000000 INFO @ Tue, 02 Aug 2022 15:11:41: 16000000 INFO @ Tue, 02 Aug 2022 15:11:41: 10000000 INFO @ Tue, 02 Aug 2022 15:11:45: 22000000 INFO @ Tue, 02 Aug 2022 15:11:47: 17000000 INFO @ Tue, 02 Aug 2022 15:11:48: 11000000 INFO @ Tue, 02 Aug 2022 15:11:51: 23000000 INFO @ Tue, 02 Aug 2022 15:11:53: 18000000 INFO @ Tue, 02 Aug 2022 15:11:54: 12000000 INFO @ Tue, 02 Aug 2022 15:11:57: 24000000 INFO @ Tue, 02 Aug 2022 15:11:59: 19000000 INFO @ Tue, 02 Aug 2022 15:12:01: 13000000 INFO @ Tue, 02 Aug 2022 15:12:03: 25000000 INFO @ Tue, 02 Aug 2022 15:12:05: 20000000 INFO @ Tue, 02 Aug 2022 15:12:08: 14000000 INFO @ Tue, 02 Aug 2022 15:12:09: 26000000 INFO @ Tue, 02 Aug 2022 15:12:10: 21000000 INFO @ Tue, 02 Aug 2022 15:12:15: 15000000 INFO @ Tue, 02 Aug 2022 15:12:15: 27000000 INFO @ Tue, 02 Aug 2022 15:12:16: 22000000 INFO @ Tue, 02 Aug 2022 15:12:21: 28000000 INFO @ Tue, 02 Aug 2022 15:12:22: 16000000 INFO @ Tue, 02 Aug 2022 15:12:22: 23000000 INFO @ Tue, 02 Aug 2022 15:12:27: 29000000 INFO @ Tue, 02 Aug 2022 15:12:28: 24000000 INFO @ Tue, 02 Aug 2022 15:12:28: 17000000 INFO @ Tue, 02 Aug 2022 15:12:33: 30000000 INFO @ Tue, 02 Aug 2022 15:12:34: 25000000 INFO @ Tue, 02 Aug 2022 15:12:35: 18000000 INFO @ Tue, 02 Aug 2022 15:12:40: 31000000 INFO @ Tue, 02 Aug 2022 15:12:40: 26000000 INFO @ Tue, 02 Aug 2022 15:12:42: 19000000 INFO @ Tue, 02 Aug 2022 15:12:46: 32000000 INFO @ Tue, 02 Aug 2022 15:12:46: 27000000 INFO @ Tue, 02 Aug 2022 15:12:48: 20000000 INFO @ Tue, 02 Aug 2022 15:12:52: 33000000 INFO @ Tue, 02 Aug 2022 15:12:52: 28000000 INFO @ Tue, 02 Aug 2022 15:12:55: 21000000 INFO @ Tue, 02 Aug 2022 15:12:58: 29000000 INFO @ Tue, 02 Aug 2022 15:12:58: 34000000 INFO @ Tue, 02 Aug 2022 15:13:02: 22000000 INFO @ Tue, 02 Aug 2022 15:13:04: 30000000 INFO @ Tue, 02 Aug 2022 15:13:04: 35000000 INFO @ Tue, 02 Aug 2022 15:13:08: 23000000 INFO @ Tue, 02 Aug 2022 15:13:10: 31000000 INFO @ Tue, 02 Aug 2022 15:13:11: 36000000 INFO @ Tue, 02 Aug 2022 15:13:15: 24000000 INFO @ Tue, 02 Aug 2022 15:13:16: 32000000 INFO @ Tue, 02 Aug 2022 15:13:17: 37000000 INFO @ Tue, 02 Aug 2022 15:13:22: 25000000 INFO @ Tue, 02 Aug 2022 15:13:22: 33000000 INFO @ Tue, 02 Aug 2022 15:13:23: 38000000 INFO @ Tue, 02 Aug 2022 15:13:28: 34000000 INFO @ Tue, 02 Aug 2022 15:13:29: 39000000 INFO @ Tue, 02 Aug 2022 15:13:29: 26000000 INFO @ Tue, 02 Aug 2022 15:13:35: 35000000 INFO @ Tue, 02 Aug 2022 15:13:35: 40000000 INFO @ Tue, 02 Aug 2022 15:13:36: 27000000 INFO @ Tue, 02 Aug 2022 15:13:41: 36000000 INFO @ Tue, 02 Aug 2022 15:13:41: 41000000 INFO @ Tue, 02 Aug 2022 15:13:43: 28000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 15:13:47: 37000000 INFO @ Tue, 02 Aug 2022 15:13:47: 42000000 INFO @ Tue, 02 Aug 2022 15:13:49: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 15:13:49: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 15:13:49: #1 total tags in treatment: 42178731 INFO @ Tue, 02 Aug 2022 15:13:49: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:13:49: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:13:49: #1 tags after filtering in treatment: 42178731 INFO @ Tue, 02 Aug 2022 15:13:49: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:13:49: #1 finished! INFO @ Tue, 02 Aug 2022 15:13:49: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:13:49: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:13:50: 29000000 INFO @ Tue, 02 Aug 2022 15:13:52: #2 number of paired peaks: 69 WARNING @ Tue, 02 Aug 2022 15:13:52: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:13:52: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:13:52: 38000000 INFO @ Tue, 02 Aug 2022 15:13:57: 30000000 INFO @ Tue, 02 Aug 2022 15:13:58: 39000000 INFO @ Tue, 02 Aug 2022 15:14:04: 31000000 INFO @ Tue, 02 Aug 2022 15:14:04: 40000000 INFO @ Tue, 02 Aug 2022 15:14:10: 41000000 INFO @ Tue, 02 Aug 2022 15:14:11: 32000000 INFO @ Tue, 02 Aug 2022 15:14:16: 42000000 INFO @ Tue, 02 Aug 2022 15:14:17: 33000000 INFO @ Tue, 02 Aug 2022 15:14:18: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 15:14:18: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 15:14:18: #1 total tags in treatment: 42178731 INFO @ Tue, 02 Aug 2022 15:14:18: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:14:18: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:14:18: #1 tags after filtering in treatment: 42178731 INFO @ Tue, 02 Aug 2022 15:14:18: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:14:18: #1 finished! INFO @ Tue, 02 Aug 2022 15:14:18: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:14:18: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:14:21: #2 number of paired peaks: 69 WARNING @ Tue, 02 Aug 2022 15:14:21: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:14:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 15:14:24: 34000000 INFO @ Tue, 02 Aug 2022 15:14:31: 35000000 INFO @ Tue, 02 Aug 2022 15:14:38: 36000000 INFO @ Tue, 02 Aug 2022 15:14:45: 37000000 INFO @ Tue, 02 Aug 2022 15:14:52: 38000000 INFO @ Tue, 02 Aug 2022 15:14:58: 39000000 BigWig に変換しました。 INFO @ Tue, 02 Aug 2022 15:15:05: 40000000 INFO @ Tue, 02 Aug 2022 15:15:12: 41000000 INFO @ Tue, 02 Aug 2022 15:15:18: 42000000 INFO @ Tue, 02 Aug 2022 15:15:20: #1 tag size is determined as 76 bps INFO @ Tue, 02 Aug 2022 15:15:20: #1 tag size = 76 INFO @ Tue, 02 Aug 2022 15:15:20: #1 total tags in treatment: 42178731 INFO @ Tue, 02 Aug 2022 15:15:20: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 15:15:20: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 15:15:21: #1 tags after filtering in treatment: 42178731 INFO @ Tue, 02 Aug 2022 15:15:21: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 02 Aug 2022 15:15:21: #1 finished! INFO @ Tue, 02 Aug 2022 15:15:21: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 15:15:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 15:15:23: #2 number of paired peaks: 69 WARNING @ Tue, 02 Aug 2022 15:15:23: Too few paired peaks (69) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 15:15:23: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX10031711/SRX10031711.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling