Job ID = 14171024 SRX = SRX10000670 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... Read 37665328 spots for SRR13606526/SRR13606526.sra Written 37665328 spots for SRR13606526/SRR13606526.sra fastq に変換しました。 bowtie でマッピング中... Your job 14171494 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:06:02 37665328 reads; of these: 37665328 (100.00%) were unpaired; of these: 26695310 (70.88%) aligned 0 times 8775982 (23.30%) aligned exactly 1 time 2194036 (5.83%) aligned >1 times 29.12% overall alignment rate Time searching: 00:06:02 Overall time: 00:06:02 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2188050 / 10970018 = 0.1995 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:23:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:23:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:23:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:23:24: 1000000 INFO @ Sat, 11 Dec 2021 09:23:29: 2000000 INFO @ Sat, 11 Dec 2021 09:23:34: 3000000 INFO @ Sat, 11 Dec 2021 09:23:39: 4000000 INFO @ Sat, 11 Dec 2021 09:23:44: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:23:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:23:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:23:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:23:49: 6000000 INFO @ Sat, 11 Dec 2021 09:23:54: 1000000 INFO @ Sat, 11 Dec 2021 09:23:54: 7000000 INFO @ Sat, 11 Dec 2021 09:23:59: 8000000 INFO @ Sat, 11 Dec 2021 09:24:00: 2000000 INFO @ Sat, 11 Dec 2021 09:24:03: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 09:24:03: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 09:24:03: #1 total tags in treatment: 8781968 INFO @ Sat, 11 Dec 2021 09:24:03: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:24:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:24:04: #1 tags after filtering in treatment: 8781968 INFO @ Sat, 11 Dec 2021 09:24:04: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:24:04: #1 finished! INFO @ Sat, 11 Dec 2021 09:24:04: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:24:04: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:24:04: #2 number of paired peaks: 1660 INFO @ Sat, 11 Dec 2021 09:24:04: start model_add_line... INFO @ Sat, 11 Dec 2021 09:24:04: start X-correlation... INFO @ Sat, 11 Dec 2021 09:24:04: end of X-cor INFO @ Sat, 11 Dec 2021 09:24:04: #2 finished! INFO @ Sat, 11 Dec 2021 09:24:04: #2 predicted fragment length is 151 bps INFO @ Sat, 11 Dec 2021 09:24:04: #2 alternative fragment length(s) may be 4,151 bps INFO @ Sat, 11 Dec 2021 09:24:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.05_model.r INFO @ Sat, 11 Dec 2021 09:24:04: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:24:04: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:24:05: 3000000 INFO @ Sat, 11 Dec 2021 09:24:11: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 09:24:16: 5000000 INFO @ Sat, 11 Dec 2021 09:24:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 09:24:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 09:24:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 09:24:21: 6000000 INFO @ Sat, 11 Dec 2021 09:24:24: 1000000 INFO @ Sat, 11 Dec 2021 09:24:25: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:24:26: 7000000 INFO @ Sat, 11 Dec 2021 09:24:30: 2000000 INFO @ Sat, 11 Dec 2021 09:24:32: 8000000 INFO @ Sat, 11 Dec 2021 09:24:36: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.05_peaks.xls INFO @ Sat, 11 Dec 2021 09:24:36: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:24:36: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.05_summits.bed INFO @ Sat, 11 Dec 2021 09:24:36: Done! INFO @ Sat, 11 Dec 2021 09:24:36: 3000000 pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2687 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 09:24:36: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 09:24:36: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 09:24:36: #1 total tags in treatment: 8781968 INFO @ Sat, 11 Dec 2021 09:24:36: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:24:36: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:24:36: #1 tags after filtering in treatment: 8781968 INFO @ Sat, 11 Dec 2021 09:24:36: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:24:36: #1 finished! INFO @ Sat, 11 Dec 2021 09:24:36: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:24:36: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:24:37: #2 number of paired peaks: 1660 INFO @ Sat, 11 Dec 2021 09:24:37: start model_add_line... INFO @ Sat, 11 Dec 2021 09:24:37: start X-correlation... INFO @ Sat, 11 Dec 2021 09:24:37: end of X-cor INFO @ Sat, 11 Dec 2021 09:24:37: #2 finished! INFO @ Sat, 11 Dec 2021 09:24:37: #2 predicted fragment length is 151 bps INFO @ Sat, 11 Dec 2021 09:24:37: #2 alternative fragment length(s) may be 4,151 bps INFO @ Sat, 11 Dec 2021 09:24:37: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.10_model.r INFO @ Sat, 11 Dec 2021 09:24:37: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:24:37: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:24:41: 4000000 INFO @ Sat, 11 Dec 2021 09:24:46: 5000000 INFO @ Sat, 11 Dec 2021 09:24:52: 6000000 INFO @ Sat, 11 Dec 2021 09:24:57: 7000000 INFO @ Sat, 11 Dec 2021 09:24:58: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 09:25:02: 8000000 INFO @ Sat, 11 Dec 2021 09:25:06: #1 tag size is determined as 51 bps INFO @ Sat, 11 Dec 2021 09:25:06: #1 tag size = 51 INFO @ Sat, 11 Dec 2021 09:25:06: #1 total tags in treatment: 8781968 INFO @ Sat, 11 Dec 2021 09:25:06: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 09:25:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 09:25:06: #1 tags after filtering in treatment: 8781968 INFO @ Sat, 11 Dec 2021 09:25:06: #1 Redundant rate of treatment: 0.00 INFO @ Sat, 11 Dec 2021 09:25:06: #1 finished! INFO @ Sat, 11 Dec 2021 09:25:06: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 09:25:06: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 09:25:07: #2 number of paired peaks: 1660 INFO @ Sat, 11 Dec 2021 09:25:07: start model_add_line... INFO @ Sat, 11 Dec 2021 09:25:07: start X-correlation... INFO @ Sat, 11 Dec 2021 09:25:07: end of X-cor INFO @ Sat, 11 Dec 2021 09:25:07: #2 finished! INFO @ Sat, 11 Dec 2021 09:25:07: #2 predicted fragment length is 151 bps INFO @ Sat, 11 Dec 2021 09:25:07: #2 alternative fragment length(s) may be 4,151 bps INFO @ Sat, 11 Dec 2021 09:25:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.20_model.r INFO @ Sat, 11 Dec 2021 09:25:07: #3 Call peaks... INFO @ Sat, 11 Dec 2021 09:25:07: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 09:25:09: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.10_peaks.xls INFO @ Sat, 11 Dec 2021 09:25:09: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:25:09: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.10_summits.bed INFO @ Sat, 11 Dec 2021 09:25:09: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (1422 records, 4 fields): 4 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 09:25:27: #3 Call peaks for each chromosome... BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 09:25:38: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.20_peaks.xls INFO @ Sat, 11 Dec 2021 09:25:38: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 09:25:38: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/SRX10000670/SRX10000670.20_summits.bed INFO @ Sat, 11 Dec 2021 09:25:38: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (720 records, 4 fields): 3 millis CompletedMACS2peakCalling