Job ID = 6527521 SRX = SRX066250 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T12:58:57 prefetch.2.10.7: 1) Downloading 'SRR222081'... 2020-06-29T12:58:57 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T12:59:58 prefetch.2.10.7: HTTPS download succeed 2020-06-29T12:59:58 prefetch.2.10.7: 'SRR222081' is valid 2020-06-29T12:59:58 prefetch.2.10.7: 1) 'SRR222081' was downloaded successfully Read 19763112 spots for SRR222081/SRR222081.sra Written 19763112 spots for SRR222081/SRR222081.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:52 19763112 reads; of these: 19763112 (100.00%) were unpaired; of these: 592372 (3.00%) aligned 0 times 14447458 (73.10%) aligned exactly 1 time 4723282 (23.90%) aligned >1 times 97.00% overall alignment rate Time searching: 00:05:53 Overall time: 00:05:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 2432783 / 19170740 = 0.1269 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:15:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:15:40: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:15:40: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:15:45: 1000000 INFO @ Mon, 29 Jun 2020 22:15:50: 2000000 INFO @ Mon, 29 Jun 2020 22:15:55: 3000000 INFO @ Mon, 29 Jun 2020 22:16:00: 4000000 INFO @ Mon, 29 Jun 2020 22:16:05: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:16:10: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:16:10: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:16:10: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:16:10: 6000000 INFO @ Mon, 29 Jun 2020 22:16:15: 1000000 INFO @ Mon, 29 Jun 2020 22:16:15: 7000000 INFO @ Mon, 29 Jun 2020 22:16:20: 2000000 INFO @ Mon, 29 Jun 2020 22:16:20: 8000000 INFO @ Mon, 29 Jun 2020 22:16:25: 3000000 INFO @ Mon, 29 Jun 2020 22:16:26: 9000000 INFO @ Mon, 29 Jun 2020 22:16:30: 4000000 INFO @ Mon, 29 Jun 2020 22:16:31: 10000000 INFO @ Mon, 29 Jun 2020 22:16:35: 5000000 INFO @ Mon, 29 Jun 2020 22:16:36: 11000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:16:40: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:16:40: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:16:40: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:16:41: 6000000 INFO @ Mon, 29 Jun 2020 22:16:41: 12000000 INFO @ Mon, 29 Jun 2020 22:16:45: 1000000 INFO @ Mon, 29 Jun 2020 22:16:46: 7000000 INFO @ Mon, 29 Jun 2020 22:16:47: 13000000 INFO @ Mon, 29 Jun 2020 22:16:51: 2000000 INFO @ Mon, 29 Jun 2020 22:16:52: 8000000 INFO @ Mon, 29 Jun 2020 22:16:52: 14000000 INFO @ Mon, 29 Jun 2020 22:16:57: 3000000 INFO @ Mon, 29 Jun 2020 22:16:57: 15000000 INFO @ Mon, 29 Jun 2020 22:16:58: 9000000 INFO @ Mon, 29 Jun 2020 22:17:02: 16000000 INFO @ Mon, 29 Jun 2020 22:17:02: 4000000 INFO @ Mon, 29 Jun 2020 22:17:03: 10000000 INFO @ Mon, 29 Jun 2020 22:17:06: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 22:17:06: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 22:17:06: #1 total tags in treatment: 16737957 INFO @ Mon, 29 Jun 2020 22:17:06: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:17:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:17:06: #1 tags after filtering in treatment: 16737957 INFO @ Mon, 29 Jun 2020 22:17:06: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:17:06: #1 finished! INFO @ Mon, 29 Jun 2020 22:17:06: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:17:06: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:17:08: #2 number of paired peaks: 42 WARNING @ Mon, 29 Jun 2020 22:17:08: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:17:08: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:17:08: 5000000 INFO @ Mon, 29 Jun 2020 22:17:09: 11000000 INFO @ Mon, 29 Jun 2020 22:17:14: 6000000 INFO @ Mon, 29 Jun 2020 22:17:14: 12000000 INFO @ Mon, 29 Jun 2020 22:17:19: 7000000 INFO @ Mon, 29 Jun 2020 22:17:20: 13000000 INFO @ Mon, 29 Jun 2020 22:17:24: 8000000 INFO @ Mon, 29 Jun 2020 22:17:25: 14000000 INFO @ Mon, 29 Jun 2020 22:17:30: 9000000 INFO @ Mon, 29 Jun 2020 22:17:31: 15000000 INFO @ Mon, 29 Jun 2020 22:17:35: 10000000 INFO @ Mon, 29 Jun 2020 22:17:36: 16000000 INFO @ Mon, 29 Jun 2020 22:17:40: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 22:17:40: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 22:17:40: #1 total tags in treatment: 16737957 INFO @ Mon, 29 Jun 2020 22:17:40: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:17:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:17:41: #1 tags after filtering in treatment: 16737957 INFO @ Mon, 29 Jun 2020 22:17:41: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:17:41: #1 finished! INFO @ Mon, 29 Jun 2020 22:17:41: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:17:41: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:17:41: 11000000 INFO @ Mon, 29 Jun 2020 22:17:42: #2 number of paired peaks: 42 WARNING @ Mon, 29 Jun 2020 22:17:42: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:17:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Mon, 29 Jun 2020 22:17:46: 12000000 INFO @ Mon, 29 Jun 2020 22:17:51: 13000000 INFO @ Mon, 29 Jun 2020 22:17:56: 14000000 INFO @ Mon, 29 Jun 2020 22:18:01: 15000000 INFO @ Mon, 29 Jun 2020 22:18:05: 16000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:18:09: #1 tag size is determined as 40 bps INFO @ Mon, 29 Jun 2020 22:18:09: #1 tag size = 40 INFO @ Mon, 29 Jun 2020 22:18:09: #1 total tags in treatment: 16737957 INFO @ Mon, 29 Jun 2020 22:18:09: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:18:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:18:10: #1 tags after filtering in treatment: 16737957 INFO @ Mon, 29 Jun 2020 22:18:10: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:18:10: #1 finished! INFO @ Mon, 29 Jun 2020 22:18:10: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:18:10: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:18:11: #2 number of paired peaks: 42 WARNING @ Mon, 29 Jun 2020 22:18:11: Too few paired peaks (42) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:18:11: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX066250/SRX066250.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。