Job ID = 9158109 sra ファイルのダウンロード中... Completed: 3753003K bytes transferred in 38 seconds (794878K bits/sec), in 7 files, 8 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 19855297 spots for /home/okishinya/chipatlas/results/dm3/SRX046653/SRR121525.sra Written 19855297 spots total Written 22962607 spots for /home/okishinya/chipatlas/results/dm3/SRX046653/SRR121526.sra Written 22962607 spots total Written 24432741 spots for /home/okishinya/chipatlas/results/dm3/SRX046653/SRR121527.sra Written 24432741 spots total Written 30911782 spots for /home/okishinya/chipatlas/results/dm3/SRX046653/SRR121528.sra Written 30911782 spots total Written 30825615 spots for /home/okishinya/chipatlas/results/dm3/SRX046653/SRR121529.sra Written 30825615 spots total Written 30800494 spots for /home/okishinya/chipatlas/results/dm3/SRX046653/SRR121531.sra Written 30800494 spots total Written 31046209 spots for /home/okishinya/chipatlas/results/dm3/SRX046653/SRR121530.sra Written 31046209 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:41:23 190834745 reads; of these: 190834745 (100.00%) were unpaired; of these: 19133863 (10.03%) aligned 0 times 146244895 (76.63%) aligned exactly 1 time 25455987 (13.34%) aligned >1 times 89.97% overall alignment rate Time searching: 00:41:24 Overall time: 00:41:24 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdupse_core] 113387851 / 171700882 = 0.6604 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 16:16:23: # Command line: callpeak -t SRX046653.bam -f BAM -g dm -n SRX046653.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX046653.20 # format = BAM # ChIP-seq file = ['SRX046653.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:16:23: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:16:23: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:16:23: # Command line: callpeak -t SRX046653.bam -f BAM -g dm -n SRX046653.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX046653.05 # format = BAM # ChIP-seq file = ['SRX046653.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:16:23: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:16:23: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:16:23: # Command line: callpeak -t SRX046653.bam -f BAM -g dm -n SRX046653.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX046653.10 # format = BAM # ChIP-seq file = ['SRX046653.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 16:16:23: #1 read tag files... INFO @ Tue, 27 Jun 2017 16:16:23: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 16:16:29: 1000000 INFO @ Tue, 27 Jun 2017 16:16:29: 1000000 INFO @ Tue, 27 Jun 2017 16:16:29: 1000000 INFO @ Tue, 27 Jun 2017 16:16:35: 2000000 INFO @ Tue, 27 Jun 2017 16:16:35: 2000000 INFO @ Tue, 27 Jun 2017 16:16:35: 2000000 INFO @ Tue, 27 Jun 2017 16:16:41: 3000000 INFO @ Tue, 27 Jun 2017 16:16:42: 3000000 INFO @ Tue, 27 Jun 2017 16:16:42: 3000000 INFO @ Tue, 27 Jun 2017 16:16:46: 4000000 INFO @ Tue, 27 Jun 2017 16:16:48: 4000000 INFO @ Tue, 27 Jun 2017 16:16:48: 4000000 INFO @ Tue, 27 Jun 2017 16:16:52: 5000000 INFO @ Tue, 27 Jun 2017 16:16:54: 5000000 INFO @ Tue, 27 Jun 2017 16:16:54: 5000000 INFO @ Tue, 27 Jun 2017 16:16:58: 6000000 INFO @ Tue, 27 Jun 2017 16:17:00: 6000000 INFO @ Tue, 27 Jun 2017 16:17:00: 6000000 INFO @ Tue, 27 Jun 2017 16:17:04: 7000000 INFO @ Tue, 27 Jun 2017 16:17:07: 7000000 INFO @ Tue, 27 Jun 2017 16:17:07: 7000000 INFO @ Tue, 27 Jun 2017 16:17:10: 8000000 INFO @ Tue, 27 Jun 2017 16:17:13: 8000000 INFO @ Tue, 27 Jun 2017 16:17:13: 8000000 INFO @ Tue, 27 Jun 2017 16:17:16: 9000000 INFO @ Tue, 27 Jun 2017 16:17:19: 9000000 INFO @ Tue, 27 Jun 2017 16:17:19: 9000000 INFO @ Tue, 27 Jun 2017 16:17:22: 10000000 INFO @ Tue, 27 Jun 2017 16:17:25: 10000000 INFO @ Tue, 27 Jun 2017 16:17:25: 10000000 INFO @ Tue, 27 Jun 2017 16:17:28: 11000000 INFO @ Tue, 27 Jun 2017 16:17:33: 11000000 INFO @ Tue, 27 Jun 2017 16:17:33: 11000000 INFO @ Tue, 27 Jun 2017 16:17:35: 12000000 INFO @ Tue, 27 Jun 2017 16:17:40: 12000000 INFO @ Tue, 27 Jun 2017 16:17:40: 12000000 INFO @ Tue, 27 Jun 2017 16:17:42: 13000000 INFO @ Tue, 27 Jun 2017 16:17:48: 13000000 INFO @ Tue, 27 Jun 2017 16:17:48: 13000000 INFO @ Tue, 27 Jun 2017 16:17:49: 14000000 INFO @ Tue, 27 Jun 2017 16:17:55: 15000000 INFO @ Tue, 27 Jun 2017 16:17:55: 14000000 INFO @ Tue, 27 Jun 2017 16:17:55: 14000000 INFO @ Tue, 27 Jun 2017 16:18:02: 16000000 INFO @ Tue, 27 Jun 2017 16:18:03: 15000000 INFO @ Tue, 27 Jun 2017 16:18:03: 15000000 INFO @ Tue, 27 Jun 2017 16:18:09: 17000000 INFO @ Tue, 27 Jun 2017 16:18:10: 16000000 INFO @ Tue, 27 Jun 2017 16:18:10: 16000000 INFO @ Tue, 27 Jun 2017 16:18:16: 18000000 INFO @ Tue, 27 Jun 2017 16:18:18: 17000000 INFO @ Tue, 27 Jun 2017 16:18:18: 17000000 INFO @ Tue, 27 Jun 2017 16:18:23: 19000000 INFO @ Tue, 27 Jun 2017 16:18:25: 18000000 INFO @ Tue, 27 Jun 2017 16:18:25: 18000000 INFO @ Tue, 27 Jun 2017 16:18:30: 20000000 INFO @ Tue, 27 Jun 2017 16:18:33: 19000000 INFO @ Tue, 27 Jun 2017 16:18:33: 19000000 INFO @ Tue, 27 Jun 2017 16:18:37: 21000000 INFO @ Tue, 27 Jun 2017 16:18:40: 20000000 INFO @ Tue, 27 Jun 2017 16:18:40: 20000000 INFO @ Tue, 27 Jun 2017 16:18:43: 22000000 INFO @ Tue, 27 Jun 2017 16:18:48: 21000000 INFO @ Tue, 27 Jun 2017 16:18:48: 21000000 INFO @ Tue, 27 Jun 2017 16:18:50: 23000000 INFO @ Tue, 27 Jun 2017 16:18:55: 22000000 INFO @ Tue, 27 Jun 2017 16:18:55: 22000000 INFO @ Tue, 27 Jun 2017 16:18:56: 24000000 INFO @ Tue, 27 Jun 2017 16:19:02: 23000000 INFO @ Tue, 27 Jun 2017 16:19:02: 23000000 INFO @ Tue, 27 Jun 2017 16:19:03: 25000000 INFO @ Tue, 27 Jun 2017 16:19:08: 24000000 INFO @ Tue, 27 Jun 2017 16:19:08: 24000000 INFO @ Tue, 27 Jun 2017 16:19:11: 26000000 INFO @ Tue, 27 Jun 2017 16:19:15: 25000000 INFO @ Tue, 27 Jun 2017 16:19:15: 25000000 INFO @ Tue, 27 Jun 2017 16:19:18: 27000000 INFO @ Tue, 27 Jun 2017 16:19:22: 26000000 INFO @ Tue, 27 Jun 2017 16:19:22: 26000000 INFO @ Tue, 27 Jun 2017 16:19:25: 28000000 INFO @ Tue, 27 Jun 2017 16:19:29: 27000000 INFO @ Tue, 27 Jun 2017 16:19:29: 27000000 INFO @ Tue, 27 Jun 2017 16:19:33: 29000000 INFO @ Tue, 27 Jun 2017 16:19:35: 28000000 INFO @ Tue, 27 Jun 2017 16:19:35: 28000000 INFO @ Tue, 27 Jun 2017 16:19:41: 30000000 INFO @ Tue, 27 Jun 2017 16:19:42: 29000000 INFO @ Tue, 27 Jun 2017 16:19:42: 29000000 INFO @ Tue, 27 Jun 2017 16:19:48: 30000000 INFO @ Tue, 27 Jun 2017 16:19:48: 30000000 INFO @ Tue, 27 Jun 2017 16:19:48: 31000000 INFO @ Tue, 27 Jun 2017 16:19:54: 31000000 INFO @ Tue, 27 Jun 2017 16:19:54: 31000000 INFO @ Tue, 27 Jun 2017 16:19:56: 32000000 INFO @ Tue, 27 Jun 2017 16:20:01: 32000000 INFO @ Tue, 27 Jun 2017 16:20:01: 32000000 INFO @ Tue, 27 Jun 2017 16:20:03: 33000000 INFO @ Tue, 27 Jun 2017 16:20:09: 33000000 INFO @ Tue, 27 Jun 2017 16:20:09: 33000000 INFO @ Tue, 27 Jun 2017 16:20:11: 34000000 INFO @ Tue, 27 Jun 2017 16:20:16: 34000000 INFO @ Tue, 27 Jun 2017 16:20:16: 34000000 INFO @ Tue, 27 Jun 2017 16:20:18: 35000000 INFO @ Tue, 27 Jun 2017 16:20:23: 35000000 INFO @ Tue, 27 Jun 2017 16:20:23: 35000000 INFO @ Tue, 27 Jun 2017 16:20:26: 36000000 INFO @ Tue, 27 Jun 2017 16:20:30: 36000000 INFO @ Tue, 27 Jun 2017 16:20:30: 36000000 INFO @ Tue, 27 Jun 2017 16:20:33: 37000000 INFO @ Tue, 27 Jun 2017 16:20:37: 37000000 INFO @ Tue, 27 Jun 2017 16:20:37: 37000000 INFO @ Tue, 27 Jun 2017 16:20:39: 38000000 INFO @ Tue, 27 Jun 2017 16:20:44: 38000000 INFO @ Tue, 27 Jun 2017 16:20:44: 38000000 INFO @ Tue, 27 Jun 2017 16:20:46: 39000000 INFO @ Tue, 27 Jun 2017 16:20:51: 39000000 INFO @ Tue, 27 Jun 2017 16:20:51: 39000000 INFO @ Tue, 27 Jun 2017 16:20:52: 40000000 INFO @ Tue, 27 Jun 2017 16:20:57: 40000000 INFO @ Tue, 27 Jun 2017 16:20:57: 40000000 INFO @ Tue, 27 Jun 2017 16:20:58: 41000000 INFO @ Tue, 27 Jun 2017 16:21:03: 41000000 INFO @ Tue, 27 Jun 2017 16:21:03: 41000000 INFO @ Tue, 27 Jun 2017 16:21:04: 42000000 INFO @ Tue, 27 Jun 2017 16:21:09: 43000000 INFO @ Tue, 27 Jun 2017 16:21:10: 42000000 INFO @ Tue, 27 Jun 2017 16:21:10: 42000000 INFO @ Tue, 27 Jun 2017 16:21:15: 44000000 INFO @ Tue, 27 Jun 2017 16:21:16: 43000000 INFO @ Tue, 27 Jun 2017 16:21:16: 43000000 INFO @ Tue, 27 Jun 2017 16:21:21: 45000000 INFO @ Tue, 27 Jun 2017 16:21:23: 44000000 INFO @ Tue, 27 Jun 2017 16:21:23: 44000000 INFO @ Tue, 27 Jun 2017 16:21:27: 46000000 INFO @ Tue, 27 Jun 2017 16:21:29: 45000000 INFO @ Tue, 27 Jun 2017 16:21:29: 45000000 INFO @ Tue, 27 Jun 2017 16:21:33: 47000000 INFO @ Tue, 27 Jun 2017 16:21:35: 46000000 INFO @ Tue, 27 Jun 2017 16:21:35: 46000000 INFO @ Tue, 27 Jun 2017 16:21:39: 48000000 INFO @ Tue, 27 Jun 2017 16:21:42: 47000000 INFO @ Tue, 27 Jun 2017 16:21:42: 47000000 INFO @ Tue, 27 Jun 2017 16:21:44: 49000000 INFO @ Tue, 27 Jun 2017 16:21:48: 48000000 INFO @ Tue, 27 Jun 2017 16:21:48: 48000000 INFO @ Tue, 27 Jun 2017 16:21:50: 50000000 INFO @ Tue, 27 Jun 2017 16:21:54: 49000000 INFO @ Tue, 27 Jun 2017 16:21:54: 49000000 INFO @ Tue, 27 Jun 2017 16:21:56: 51000000 INFO @ Tue, 27 Jun 2017 16:22:01: 50000000 INFO @ Tue, 27 Jun 2017 16:22:01: 50000000 INFO @ Tue, 27 Jun 2017 16:22:02: 52000000 INFO @ Tue, 27 Jun 2017 16:22:07: 51000000 INFO @ Tue, 27 Jun 2017 16:22:07: 51000000 INFO @ Tue, 27 Jun 2017 16:22:08: 53000000 INFO @ Tue, 27 Jun 2017 16:22:13: 52000000 INFO @ Tue, 27 Jun 2017 16:22:13: 52000000 INFO @ Tue, 27 Jun 2017 16:22:13: 54000000 INFO @ Tue, 27 Jun 2017 16:22:19: 55000000 INFO @ Tue, 27 Jun 2017 16:22:19: 53000000 INFO @ Tue, 27 Jun 2017 16:22:19: 53000000 INFO @ Tue, 27 Jun 2017 16:22:25: 56000000 INFO @ Tue, 27 Jun 2017 16:22:26: 54000000 INFO @ Tue, 27 Jun 2017 16:22:26: 54000000 INFO @ Tue, 27 Jun 2017 16:22:31: 57000000 INFO @ Tue, 27 Jun 2017 16:22:32: 55000000 INFO @ Tue, 27 Jun 2017 16:22:32: 55000000 INFO @ Tue, 27 Jun 2017 16:22:36: 58000000 INFO @ Tue, 27 Jun 2017 16:22:38: 56000000 INFO @ Tue, 27 Jun 2017 16:22:38: 56000000 INFO @ Tue, 27 Jun 2017 16:22:39: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 16:22:39: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 16:22:39: #1 total tags in treatment: 58313031 INFO @ Tue, 27 Jun 2017 16:22:39: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:22:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:22:40: #1 tags after filtering in treatment: 58313031 INFO @ Tue, 27 Jun 2017 16:22:40: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:22:40: #1 finished! INFO @ Tue, 27 Jun 2017 16:22:40: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:22:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:22:44: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:22:44: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:22:44: Process for pairing-model is terminated! cat: SRX046653.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX046653.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX046653.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX046653.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:22:44: 57000000 INFO @ Tue, 27 Jun 2017 16:22:44: 57000000 INFO @ Tue, 27 Jun 2017 16:22:50: 58000000 INFO @ Tue, 27 Jun 2017 16:22:50: 58000000 INFO @ Tue, 27 Jun 2017 16:22:53: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 16:22:53: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 16:22:53: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 16:22:53: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 16:22:53: #1 total tags in treatment: 58313031 INFO @ Tue, 27 Jun 2017 16:22:53: #1 total tags in treatment: 58313031 INFO @ Tue, 27 Jun 2017 16:22:53: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:22:53: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 16:22:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:22:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 16:22:54: #1 tags after filtering in treatment: 58313031 INFO @ Tue, 27 Jun 2017 16:22:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:22:54: #1 finished! INFO @ Tue, 27 Jun 2017 16:22:54: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:22:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:22:54: #1 tags after filtering in treatment: 58313031 INFO @ Tue, 27 Jun 2017 16:22:54: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 16:22:54: #1 finished! INFO @ Tue, 27 Jun 2017 16:22:54: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 16:22:54: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 16:22:58: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:22:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:22:58: Process for pairing-model is terminated! cat: SRX046653.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX046653.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX046653.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX046653.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 16:22:58: #2 number of paired peaks: 0 WARNING @ Tue, 27 Jun 2017 16:22:58: Too few paired peaks (0) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 16:22:58: Process for pairing-model is terminated! cat: SRX046653.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX046653.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX046653.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX046653.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。