Job ID = 9158078 sra ファイルのダウンロード中... Completed: 707760K bytes transferred in 8 seconds (644782K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 14795589 spots for /home/okishinya/chipatlas/results/dm3/SRX033323/SRR080720.sra Written 14795589 spots total Written 16096991 spots for /home/okishinya/chipatlas/results/dm3/SRX033323/SRR080719.sra Written 16096991 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:56 30892580 reads; of these: 30892580 (100.00%) were unpaired; of these: 1534900 (4.97%) aligned 0 times 23193651 (75.08%) aligned exactly 1 time 6164029 (19.95%) aligned >1 times 95.03% overall alignment rate Time searching: 00:08:56 Overall time: 00:08:56 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6740161 / 29357680 = 0.2296 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:08:47: # Command line: callpeak -t SRX033323.bam -f BAM -g dm -n SRX033323.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX033323.05 # format = BAM # ChIP-seq file = ['SRX033323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:08:47: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:08:47: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:08:47: # Command line: callpeak -t SRX033323.bam -f BAM -g dm -n SRX033323.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX033323.10 # format = BAM # ChIP-seq file = ['SRX033323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:08:47: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:08:47: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:08:47: # Command line: callpeak -t SRX033323.bam -f BAM -g dm -n SRX033323.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX033323.20 # format = BAM # ChIP-seq file = ['SRX033323.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:08:47: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:08:47: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:08:56: 1000000 INFO @ Tue, 27 Jun 2017 15:08:56: 1000000 INFO @ Tue, 27 Jun 2017 15:08:56: 1000000 INFO @ Tue, 27 Jun 2017 15:09:04: 2000000 INFO @ Tue, 27 Jun 2017 15:09:04: 2000000 INFO @ Tue, 27 Jun 2017 15:09:04: 2000000 INFO @ Tue, 27 Jun 2017 15:09:13: 3000000 INFO @ Tue, 27 Jun 2017 15:09:13: 3000000 INFO @ Tue, 27 Jun 2017 15:09:13: 3000000 INFO @ Tue, 27 Jun 2017 15:09:22: 4000000 INFO @ Tue, 27 Jun 2017 15:09:22: 4000000 INFO @ Tue, 27 Jun 2017 15:09:22: 4000000 INFO @ Tue, 27 Jun 2017 15:09:30: 5000000 INFO @ Tue, 27 Jun 2017 15:09:30: 5000000 INFO @ Tue, 27 Jun 2017 15:09:30: 5000000 INFO @ Tue, 27 Jun 2017 15:09:39: 6000000 INFO @ Tue, 27 Jun 2017 15:09:39: 6000000 INFO @ Tue, 27 Jun 2017 15:09:39: 6000000 INFO @ Tue, 27 Jun 2017 15:09:47: 7000000 INFO @ Tue, 27 Jun 2017 15:09:47: 7000000 INFO @ Tue, 27 Jun 2017 15:09:47: 7000000 INFO @ Tue, 27 Jun 2017 15:09:56: 8000000 INFO @ Tue, 27 Jun 2017 15:09:56: 8000000 INFO @ Tue, 27 Jun 2017 15:09:56: 8000000 INFO @ Tue, 27 Jun 2017 15:10:04: 9000000 INFO @ Tue, 27 Jun 2017 15:10:04: 9000000 INFO @ Tue, 27 Jun 2017 15:10:04: 9000000 INFO @ Tue, 27 Jun 2017 15:10:12: 10000000 INFO @ Tue, 27 Jun 2017 15:10:12: 10000000 INFO @ Tue, 27 Jun 2017 15:10:12: 10000000 INFO @ Tue, 27 Jun 2017 15:10:20: 11000000 INFO @ Tue, 27 Jun 2017 15:10:20: 11000000 INFO @ Tue, 27 Jun 2017 15:10:20: 11000000 INFO @ Tue, 27 Jun 2017 15:10:28: 12000000 INFO @ Tue, 27 Jun 2017 15:10:28: 12000000 INFO @ Tue, 27 Jun 2017 15:10:28: 12000000 INFO @ Tue, 27 Jun 2017 15:10:36: 13000000 INFO @ Tue, 27 Jun 2017 15:10:36: 13000000 INFO @ Tue, 27 Jun 2017 15:10:36: 13000000 INFO @ Tue, 27 Jun 2017 15:10:44: 14000000 INFO @ Tue, 27 Jun 2017 15:10:44: 14000000 INFO @ Tue, 27 Jun 2017 15:10:44: 14000000 INFO @ Tue, 27 Jun 2017 15:10:52: 15000000 INFO @ Tue, 27 Jun 2017 15:10:52: 15000000 INFO @ Tue, 27 Jun 2017 15:10:52: 15000000 INFO @ Tue, 27 Jun 2017 15:11:00: 16000000 INFO @ Tue, 27 Jun 2017 15:11:00: 16000000 INFO @ Tue, 27 Jun 2017 15:11:00: 16000000 INFO @ Tue, 27 Jun 2017 15:11:07: 17000000 INFO @ Tue, 27 Jun 2017 15:11:07: 17000000 INFO @ Tue, 27 Jun 2017 15:11:07: 17000000 INFO @ Tue, 27 Jun 2017 15:11:15: 18000000 INFO @ Tue, 27 Jun 2017 15:11:15: 18000000 INFO @ Tue, 27 Jun 2017 15:11:15: 18000000 INFO @ Tue, 27 Jun 2017 15:11:24: 19000000 INFO @ Tue, 27 Jun 2017 15:11:24: 19000000 INFO @ Tue, 27 Jun 2017 15:11:24: 19000000 INFO @ Tue, 27 Jun 2017 15:11:32: 20000000 INFO @ Tue, 27 Jun 2017 15:11:32: 20000000 INFO @ Tue, 27 Jun 2017 15:11:32: 20000000 INFO @ Tue, 27 Jun 2017 15:11:40: 21000000 INFO @ Tue, 27 Jun 2017 15:11:40: 21000000 INFO @ Tue, 27 Jun 2017 15:11:40: 21000000 INFO @ Tue, 27 Jun 2017 15:11:48: 22000000 INFO @ Tue, 27 Jun 2017 15:11:48: 22000000 INFO @ Tue, 27 Jun 2017 15:11:48: 22000000 INFO @ Tue, 27 Jun 2017 15:11:53: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:11:53: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:11:53: #1 total tags in treatment: 22617519 INFO @ Tue, 27 Jun 2017 15:11:53: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:11:53: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:11:53: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:11:53: #1 total tags in treatment: 22617519 INFO @ Tue, 27 Jun 2017 15:11:53: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:11:53: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:11:53: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:11:53: #1 total tags in treatment: 22617519 INFO @ Tue, 27 Jun 2017 15:11:53: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:11:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:11:53: #1 tags after filtering in treatment: 22617519 INFO @ Tue, 27 Jun 2017 15:11:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:11:53: #1 finished! INFO @ Tue, 27 Jun 2017 15:11:53: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:11:53: #1 tags after filtering in treatment: 22617519 INFO @ Tue, 27 Jun 2017 15:11:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:11:53: #1 finished! INFO @ Tue, 27 Jun 2017 15:11:53: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:11:53: #1 tags after filtering in treatment: 22617519 INFO @ Tue, 27 Jun 2017 15:11:53: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:11:53: #1 finished! INFO @ Tue, 27 Jun 2017 15:11:53: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:11:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:11:55: #2 number of paired peaks: 36 WARNING @ Tue, 27 Jun 2017 15:11:55: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:11:55: Process for pairing-model is terminated! cat: SRX033323.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033323.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033323.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033323.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:11:55: #2 number of paired peaks: 36 WARNING @ Tue, 27 Jun 2017 15:11:55: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:11:55: Process for pairing-model is terminated! cat: SRX033323.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033323.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033323.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033323.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:11:55: #2 number of paired peaks: 36 WARNING @ Tue, 27 Jun 2017 15:11:55: Too few paired peaks (36) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:11:55: Process for pairing-model is terminated! cat: SRX033323.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033323.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033323.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033323.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。