Job ID = 9158074 sra ファイルのダウンロード中... Completed: 866413K bytes transferred in 10 seconds (647169K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 22513368 spots for /home/okishinya/chipatlas/results/dm3/SRX033318/SRR080713.sra Written 22513368 spots total Written 27050643 spots for /home/okishinya/chipatlas/results/dm3/SRX033318/SRR080712.sra Written 27050643 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:19:30 49564011 reads; of these: 49564011 (100.00%) were unpaired; of these: 629005 (1.27%) aligned 0 times 34820616 (70.25%) aligned exactly 1 time 14114390 (28.48%) aligned >1 times 98.73% overall alignment rate Time searching: 00:19:30 Overall time: 00:19:30 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 20557350 / 48935006 = 0.4201 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:19:01: # Command line: callpeak -t SRX033318.bam -f BAM -g dm -n SRX033318.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX033318.10 # format = BAM # ChIP-seq file = ['SRX033318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:19:01: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:19:01: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:19:01: # Command line: callpeak -t SRX033318.bam -f BAM -g dm -n SRX033318.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX033318.20 # format = BAM # ChIP-seq file = ['SRX033318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:19:01: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:19:01: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:19:01: # Command line: callpeak -t SRX033318.bam -f BAM -g dm -n SRX033318.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX033318.05 # format = BAM # ChIP-seq file = ['SRX033318.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:19:01: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:19:01: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:19:08: 1000000 INFO @ Tue, 27 Jun 2017 15:19:08: 1000000 INFO @ Tue, 27 Jun 2017 15:19:08: 1000000 INFO @ Tue, 27 Jun 2017 15:19:16: 2000000 INFO @ Tue, 27 Jun 2017 15:19:16: 2000000 INFO @ Tue, 27 Jun 2017 15:19:16: 2000000 INFO @ Tue, 27 Jun 2017 15:19:23: 3000000 INFO @ Tue, 27 Jun 2017 15:19:23: 3000000 INFO @ Tue, 27 Jun 2017 15:19:24: 3000000 INFO @ Tue, 27 Jun 2017 15:19:30: 4000000 INFO @ Tue, 27 Jun 2017 15:19:30: 4000000 INFO @ Tue, 27 Jun 2017 15:19:31: 4000000 INFO @ Tue, 27 Jun 2017 15:19:38: 5000000 INFO @ Tue, 27 Jun 2017 15:19:38: 5000000 INFO @ Tue, 27 Jun 2017 15:19:39: 5000000 INFO @ Tue, 27 Jun 2017 15:19:45: 6000000 INFO @ Tue, 27 Jun 2017 15:19:45: 6000000 INFO @ Tue, 27 Jun 2017 15:19:46: 6000000 INFO @ Tue, 27 Jun 2017 15:19:52: 7000000 INFO @ Tue, 27 Jun 2017 15:19:52: 7000000 INFO @ Tue, 27 Jun 2017 15:19:54: 7000000 INFO @ Tue, 27 Jun 2017 15:20:00: 8000000 INFO @ Tue, 27 Jun 2017 15:20:00: 8000000 INFO @ Tue, 27 Jun 2017 15:20:02: 8000000 INFO @ Tue, 27 Jun 2017 15:20:07: 9000000 INFO @ Tue, 27 Jun 2017 15:20:07: 9000000 INFO @ Tue, 27 Jun 2017 15:20:09: 9000000 INFO @ Tue, 27 Jun 2017 15:20:15: 10000000 INFO @ Tue, 27 Jun 2017 15:20:15: 10000000 INFO @ Tue, 27 Jun 2017 15:20:17: 10000000 INFO @ Tue, 27 Jun 2017 15:20:22: 11000000 INFO @ Tue, 27 Jun 2017 15:20:22: 11000000 INFO @ Tue, 27 Jun 2017 15:20:25: 11000000 INFO @ Tue, 27 Jun 2017 15:20:30: 12000000 INFO @ Tue, 27 Jun 2017 15:20:30: 12000000 INFO @ Tue, 27 Jun 2017 15:20:32: 12000000 INFO @ Tue, 27 Jun 2017 15:20:37: 13000000 INFO @ Tue, 27 Jun 2017 15:20:37: 13000000 INFO @ Tue, 27 Jun 2017 15:20:40: 13000000 INFO @ Tue, 27 Jun 2017 15:20:45: 14000000 INFO @ Tue, 27 Jun 2017 15:20:45: 14000000 INFO @ Tue, 27 Jun 2017 15:20:47: 14000000 INFO @ Tue, 27 Jun 2017 15:20:52: 15000000 INFO @ Tue, 27 Jun 2017 15:20:52: 15000000 INFO @ Tue, 27 Jun 2017 15:20:55: 15000000 INFO @ Tue, 27 Jun 2017 15:21:00: 16000000 INFO @ Tue, 27 Jun 2017 15:21:00: 16000000 INFO @ Tue, 27 Jun 2017 15:21:03: 16000000 INFO @ Tue, 27 Jun 2017 15:21:07: 17000000 INFO @ Tue, 27 Jun 2017 15:21:07: 17000000 INFO @ Tue, 27 Jun 2017 15:21:10: 17000000 INFO @ Tue, 27 Jun 2017 15:21:14: 18000000 INFO @ Tue, 27 Jun 2017 15:21:14: 18000000 INFO @ Tue, 27 Jun 2017 15:21:17: 18000000 INFO @ Tue, 27 Jun 2017 15:21:21: 19000000 INFO @ Tue, 27 Jun 2017 15:21:21: 19000000 INFO @ Tue, 27 Jun 2017 15:21:24: 19000000 INFO @ Tue, 27 Jun 2017 15:21:28: 20000000 INFO @ Tue, 27 Jun 2017 15:21:28: 20000000 INFO @ Tue, 27 Jun 2017 15:21:32: 20000000 INFO @ Tue, 27 Jun 2017 15:21:35: 21000000 INFO @ Tue, 27 Jun 2017 15:21:35: 21000000 INFO @ Tue, 27 Jun 2017 15:21:39: 21000000 INFO @ Tue, 27 Jun 2017 15:21:41: 22000000 INFO @ Tue, 27 Jun 2017 15:21:41: 22000000 INFO @ Tue, 27 Jun 2017 15:21:46: 22000000 INFO @ Tue, 27 Jun 2017 15:21:48: 23000000 INFO @ Tue, 27 Jun 2017 15:21:48: 23000000 INFO @ Tue, 27 Jun 2017 15:21:53: 23000000 INFO @ Tue, 27 Jun 2017 15:21:55: 24000000 INFO @ Tue, 27 Jun 2017 15:21:55: 24000000 INFO @ Tue, 27 Jun 2017 15:22:00: 24000000 INFO @ Tue, 27 Jun 2017 15:22:02: 25000000 INFO @ Tue, 27 Jun 2017 15:22:02: 25000000 INFO @ Tue, 27 Jun 2017 15:22:07: 25000000 INFO @ Tue, 27 Jun 2017 15:22:08: 26000000 INFO @ Tue, 27 Jun 2017 15:22:08: 26000000 INFO @ Tue, 27 Jun 2017 15:22:14: 26000000 INFO @ Tue, 27 Jun 2017 15:22:15: 27000000 INFO @ Tue, 27 Jun 2017 15:22:15: 27000000 INFO @ Tue, 27 Jun 2017 15:22:21: 27000000 INFO @ Tue, 27 Jun 2017 15:22:22: 28000000 INFO @ Tue, 27 Jun 2017 15:22:22: 28000000 INFO @ Tue, 27 Jun 2017 15:22:25: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:22:25: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:22:25: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:22:25: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:22:25: #1 total tags in treatment: 28377656 INFO @ Tue, 27 Jun 2017 15:22:25: #1 total tags in treatment: 28377656 INFO @ Tue, 27 Jun 2017 15:22:25: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:22:25: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:22:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:22:25: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:22:25: #1 tags after filtering in treatment: 28377656 INFO @ Tue, 27 Jun 2017 15:22:25: #1 tags after filtering in treatment: 28377656 INFO @ Tue, 27 Jun 2017 15:22:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:22:25: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:22:25: #1 finished! INFO @ Tue, 27 Jun 2017 15:22:25: #1 finished! INFO @ Tue, 27 Jun 2017 15:22:25: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:22:25: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:22:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:22:25: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:22:27: #2 number of paired peaks: 93 WARNING @ Tue, 27 Jun 2017 15:22:27: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:22:27: Process for pairing-model is terminated! cat: SRX033318.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033318.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033318.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033318.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:22:27: #2 number of paired peaks: 93 WARNING @ Tue, 27 Jun 2017 15:22:27: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:22:27: Process for pairing-model is terminated! cat: SRX033318.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033318.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033318.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033318.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:22:28: 28000000 INFO @ Tue, 27 Jun 2017 15:22:31: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:22:31: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:22:31: #1 total tags in treatment: 28377656 INFO @ Tue, 27 Jun 2017 15:22:31: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:22:31: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:22:32: #1 tags after filtering in treatment: 28377656 INFO @ Tue, 27 Jun 2017 15:22:32: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:22:32: #1 finished! INFO @ Tue, 27 Jun 2017 15:22:32: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:22:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:22:34: #2 number of paired peaks: 93 WARNING @ Tue, 27 Jun 2017 15:22:34: Too few paired peaks (93) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:22:34: Process for pairing-model is terminated! cat: SRX033318.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033318.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033318.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033318.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。