Job ID = 9158072 sra ファイルのダウンロード中... Completed: 1113084K bytes transferred in 14 seconds (649991K bits/sec), in 2 files, 3 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 25675302 spots for /home/okishinya/chipatlas/results/dm3/SRX033313/SRR080705.sra Written 25675302 spots total Written 29456758 spots for /home/okishinya/chipatlas/results/dm3/SRX033313/SRR080706.sra Written 29456758 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:13:21 55132060 reads; of these: 55132060 (100.00%) were unpaired; of these: 2314468 (4.20%) aligned 0 times 46277164 (83.94%) aligned exactly 1 time 6540428 (11.86%) aligned >1 times 95.80% overall alignment rate Time searching: 00:13:21 Overall time: 00:13:21 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 16 files... [bam_rmdupse_core] 13761708 / 52817592 = 0.2606 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:14:41: # Command line: callpeak -t SRX033313.bam -f BAM -g dm -n SRX033313.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX033313.05 # format = BAM # ChIP-seq file = ['SRX033313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:14:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:14:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:14:41: # Command line: callpeak -t SRX033313.bam -f BAM -g dm -n SRX033313.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX033313.10 # format = BAM # ChIP-seq file = ['SRX033313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:14:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:14:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:14:41: # Command line: callpeak -t SRX033313.bam -f BAM -g dm -n SRX033313.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX033313.20 # format = BAM # ChIP-seq file = ['SRX033313.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:14:41: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:14:41: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:14:49: 1000000 INFO @ Tue, 27 Jun 2017 15:14:50: 1000000 INFO @ Tue, 27 Jun 2017 15:14:50: 1000000 INFO @ Tue, 27 Jun 2017 15:14:56: 2000000 INFO @ Tue, 27 Jun 2017 15:14:58: 2000000 INFO @ Tue, 27 Jun 2017 15:14:58: 2000000 INFO @ Tue, 27 Jun 2017 15:15:04: 3000000 INFO @ Tue, 27 Jun 2017 15:15:07: 3000000 INFO @ Tue, 27 Jun 2017 15:15:07: 3000000 INFO @ Tue, 27 Jun 2017 15:15:12: 4000000 INFO @ Tue, 27 Jun 2017 15:15:16: 4000000 INFO @ Tue, 27 Jun 2017 15:15:16: 4000000 INFO @ Tue, 27 Jun 2017 15:15:19: 5000000 INFO @ Tue, 27 Jun 2017 15:15:24: 5000000 INFO @ Tue, 27 Jun 2017 15:15:24: 5000000 INFO @ Tue, 27 Jun 2017 15:15:27: 6000000 INFO @ Tue, 27 Jun 2017 15:15:33: 6000000 INFO @ Tue, 27 Jun 2017 15:15:33: 6000000 INFO @ Tue, 27 Jun 2017 15:15:34: 7000000 INFO @ Tue, 27 Jun 2017 15:15:41: 8000000 INFO @ Tue, 27 Jun 2017 15:15:41: 7000000 INFO @ Tue, 27 Jun 2017 15:15:41: 7000000 INFO @ Tue, 27 Jun 2017 15:15:48: 9000000 INFO @ Tue, 27 Jun 2017 15:15:49: 8000000 INFO @ Tue, 27 Jun 2017 15:15:49: 8000000 INFO @ Tue, 27 Jun 2017 15:15:54: 10000000 INFO @ Tue, 27 Jun 2017 15:15:56: 9000000 INFO @ Tue, 27 Jun 2017 15:15:56: 9000000 INFO @ Tue, 27 Jun 2017 15:16:01: 11000000 INFO @ Tue, 27 Jun 2017 15:16:03: 10000000 INFO @ Tue, 27 Jun 2017 15:16:03: 10000000 INFO @ Tue, 27 Jun 2017 15:16:07: 12000000 INFO @ Tue, 27 Jun 2017 15:16:10: 11000000 INFO @ Tue, 27 Jun 2017 15:16:10: 11000000 INFO @ Tue, 27 Jun 2017 15:16:14: 13000000 INFO @ Tue, 27 Jun 2017 15:16:17: 12000000 INFO @ Tue, 27 Jun 2017 15:16:17: 12000000 INFO @ Tue, 27 Jun 2017 15:16:20: 14000000 INFO @ Tue, 27 Jun 2017 15:16:24: 13000000 INFO @ Tue, 27 Jun 2017 15:16:24: 13000000 INFO @ Tue, 27 Jun 2017 15:16:27: 15000000 INFO @ Tue, 27 Jun 2017 15:16:31: 14000000 INFO @ Tue, 27 Jun 2017 15:16:31: 14000000 INFO @ Tue, 27 Jun 2017 15:16:33: 16000000 INFO @ Tue, 27 Jun 2017 15:16:38: 15000000 INFO @ Tue, 27 Jun 2017 15:16:38: 15000000 INFO @ Tue, 27 Jun 2017 15:16:40: 17000000 INFO @ Tue, 27 Jun 2017 15:16:45: 16000000 INFO @ Tue, 27 Jun 2017 15:16:45: 16000000 INFO @ Tue, 27 Jun 2017 15:16:47: 18000000 INFO @ Tue, 27 Jun 2017 15:16:52: 17000000 INFO @ Tue, 27 Jun 2017 15:16:52: 17000000 INFO @ Tue, 27 Jun 2017 15:16:53: 19000000 INFO @ Tue, 27 Jun 2017 15:17:00: 18000000 INFO @ Tue, 27 Jun 2017 15:17:00: 18000000 INFO @ Tue, 27 Jun 2017 15:17:00: 20000000 INFO @ Tue, 27 Jun 2017 15:17:06: 21000000 INFO @ Tue, 27 Jun 2017 15:17:06: 19000000 INFO @ Tue, 27 Jun 2017 15:17:06: 19000000 INFO @ Tue, 27 Jun 2017 15:17:13: 22000000 INFO @ Tue, 27 Jun 2017 15:17:13: 20000000 INFO @ Tue, 27 Jun 2017 15:17:13: 20000000 INFO @ Tue, 27 Jun 2017 15:17:19: 23000000 INFO @ Tue, 27 Jun 2017 15:17:20: 21000000 INFO @ Tue, 27 Jun 2017 15:17:20: 21000000 INFO @ Tue, 27 Jun 2017 15:17:26: 24000000 INFO @ Tue, 27 Jun 2017 15:17:27: 22000000 INFO @ Tue, 27 Jun 2017 15:17:27: 22000000 INFO @ Tue, 27 Jun 2017 15:17:32: 25000000 INFO @ Tue, 27 Jun 2017 15:17:34: 23000000 INFO @ Tue, 27 Jun 2017 15:17:34: 23000000 INFO @ Tue, 27 Jun 2017 15:17:39: 26000000 INFO @ Tue, 27 Jun 2017 15:17:42: 24000000 INFO @ Tue, 27 Jun 2017 15:17:42: 24000000 INFO @ Tue, 27 Jun 2017 15:17:46: 27000000 INFO @ Tue, 27 Jun 2017 15:17:49: 25000000 INFO @ Tue, 27 Jun 2017 15:17:49: 25000000 INFO @ Tue, 27 Jun 2017 15:17:52: 28000000 INFO @ Tue, 27 Jun 2017 15:17:56: 26000000 INFO @ Tue, 27 Jun 2017 15:17:56: 26000000 INFO @ Tue, 27 Jun 2017 15:17:59: 29000000 INFO @ Tue, 27 Jun 2017 15:18:03: 27000000 INFO @ Tue, 27 Jun 2017 15:18:03: 27000000 INFO @ Tue, 27 Jun 2017 15:18:05: 30000000 INFO @ Tue, 27 Jun 2017 15:18:10: 28000000 INFO @ Tue, 27 Jun 2017 15:18:10: 28000000 INFO @ Tue, 27 Jun 2017 15:18:12: 31000000 INFO @ Tue, 27 Jun 2017 15:18:17: 29000000 INFO @ Tue, 27 Jun 2017 15:18:17: 29000000 INFO @ Tue, 27 Jun 2017 15:18:18: 32000000 INFO @ Tue, 27 Jun 2017 15:18:25: 30000000 INFO @ Tue, 27 Jun 2017 15:18:25: 30000000 INFO @ Tue, 27 Jun 2017 15:18:25: 33000000 INFO @ Tue, 27 Jun 2017 15:18:31: 34000000 INFO @ Tue, 27 Jun 2017 15:18:32: 31000000 INFO @ Tue, 27 Jun 2017 15:18:32: 31000000 INFO @ Tue, 27 Jun 2017 15:18:38: 35000000 INFO @ Tue, 27 Jun 2017 15:18:39: 32000000 INFO @ Tue, 27 Jun 2017 15:18:39: 32000000 INFO @ Tue, 27 Jun 2017 15:18:44: 36000000 INFO @ Tue, 27 Jun 2017 15:18:46: 33000000 INFO @ Tue, 27 Jun 2017 15:18:46: 33000000 INFO @ Tue, 27 Jun 2017 15:18:51: 37000000 INFO @ Tue, 27 Jun 2017 15:18:53: 34000000 INFO @ Tue, 27 Jun 2017 15:18:53: 34000000 INFO @ Tue, 27 Jun 2017 15:18:57: 38000000 INFO @ Tue, 27 Jun 2017 15:19:01: 35000000 INFO @ Tue, 27 Jun 2017 15:19:01: 35000000 INFO @ Tue, 27 Jun 2017 15:19:04: 39000000 INFO @ Tue, 27 Jun 2017 15:19:05: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:19:05: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:19:05: #1 total tags in treatment: 39055884 INFO @ Tue, 27 Jun 2017 15:19:05: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:19:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:19:05: #1 tags after filtering in treatment: 39055884 INFO @ Tue, 27 Jun 2017 15:19:05: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:19:05: #1 finished! INFO @ Tue, 27 Jun 2017 15:19:05: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:19:05: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:19:08: 36000000 INFO @ Tue, 27 Jun 2017 15:19:08: #2 number of paired peaks: 11 WARNING @ Tue, 27 Jun 2017 15:19:08: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:19:08: Process for pairing-model is terminated! INFO @ Tue, 27 Jun 2017 15:19:08: 36000000 cat: SRX033313.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033313.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033313.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033313.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:19:15: 37000000 INFO @ Tue, 27 Jun 2017 15:19:15: 37000000 INFO @ Tue, 27 Jun 2017 15:19:22: 38000000 INFO @ Tue, 27 Jun 2017 15:19:22: 38000000 INFO @ Tue, 27 Jun 2017 15:19:29: 39000000 INFO @ Tue, 27 Jun 2017 15:19:29: 39000000 INFO @ Tue, 27 Jun 2017 15:19:29: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:19:29: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:19:29: #1 total tags in treatment: 39055884 INFO @ Tue, 27 Jun 2017 15:19:29: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:19:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:19:29: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:19:29: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:19:29: #1 total tags in treatment: 39055884 INFO @ Tue, 27 Jun 2017 15:19:29: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:19:29: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:19:30: #1 tags after filtering in treatment: 39055884 INFO @ Tue, 27 Jun 2017 15:19:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:19:30: #1 finished! INFO @ Tue, 27 Jun 2017 15:19:30: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:19:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:19:30: #1 tags after filtering in treatment: 39055884 INFO @ Tue, 27 Jun 2017 15:19:30: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:19:30: #1 finished! INFO @ Tue, 27 Jun 2017 15:19:30: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:19:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:19:33: #2 number of paired peaks: 11 WARNING @ Tue, 27 Jun 2017 15:19:33: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:19:33: Process for pairing-model is terminated! cat: SRX033313.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033313.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033313.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033313.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:19:33: #2 number of paired peaks: 11 WARNING @ Tue, 27 Jun 2017 15:19:33: Too few paired peaks (11) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:19:33: Process for pairing-model is terminated! cat: SRX033313.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX033313.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033313.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX033313.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。