Job ID = 9158064 sra ファイルのダウンロード中... Completed: 567492K bytes transferred in 8 seconds (574719K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 28798960 spots for /home/okishinya/chipatlas/results/dm3/SRX030961/SRR072389.sra Written 28798960 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:08:52 28798960 reads; of these: 28798960 (100.00%) were unpaired; of these: 1444701 (5.02%) aligned 0 times 22045222 (76.55%) aligned exactly 1 time 5309037 (18.43%) aligned >1 times 94.98% overall alignment rate Time searching: 00:08:52 Overall time: 00:08:52 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 3736803 / 27354259 = 0.1366 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:02:17: # Command line: callpeak -t SRX030961.bam -f BAM -g dm -n SRX030961.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX030961.10 # format = BAM # ChIP-seq file = ['SRX030961.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:02:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:02:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:02:17: # Command line: callpeak -t SRX030961.bam -f BAM -g dm -n SRX030961.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX030961.20 # format = BAM # ChIP-seq file = ['SRX030961.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:02:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:02:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:02:17: # Command line: callpeak -t SRX030961.bam -f BAM -g dm -n SRX030961.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX030961.05 # format = BAM # ChIP-seq file = ['SRX030961.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:02:17: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:02:17: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:02:23: 1000000 INFO @ Tue, 27 Jun 2017 15:02:24: 1000000 INFO @ Tue, 27 Jun 2017 15:02:24: 1000000 INFO @ Tue, 27 Jun 2017 15:02:30: 2000000 INFO @ Tue, 27 Jun 2017 15:02:30: 2000000 INFO @ Tue, 27 Jun 2017 15:02:30: 2000000 INFO @ Tue, 27 Jun 2017 15:02:36: 3000000 INFO @ Tue, 27 Jun 2017 15:02:37: 3000000 INFO @ Tue, 27 Jun 2017 15:02:37: 3000000 INFO @ Tue, 27 Jun 2017 15:02:43: 4000000 INFO @ Tue, 27 Jun 2017 15:02:44: 4000000 INFO @ Tue, 27 Jun 2017 15:02:44: 4000000 INFO @ Tue, 27 Jun 2017 15:02:49: 5000000 INFO @ Tue, 27 Jun 2017 15:02:50: 5000000 INFO @ Tue, 27 Jun 2017 15:02:50: 5000000 INFO @ Tue, 27 Jun 2017 15:02:56: 6000000 INFO @ Tue, 27 Jun 2017 15:02:57: 6000000 INFO @ Tue, 27 Jun 2017 15:02:57: 6000000 INFO @ Tue, 27 Jun 2017 15:03:02: 7000000 INFO @ Tue, 27 Jun 2017 15:03:04: 7000000 INFO @ Tue, 27 Jun 2017 15:03:04: 7000000 INFO @ Tue, 27 Jun 2017 15:03:09: 8000000 INFO @ Tue, 27 Jun 2017 15:03:10: 8000000 INFO @ Tue, 27 Jun 2017 15:03:10: 8000000 INFO @ Tue, 27 Jun 2017 15:03:15: 9000000 INFO @ Tue, 27 Jun 2017 15:03:17: 9000000 INFO @ Tue, 27 Jun 2017 15:03:17: 9000000 INFO @ Tue, 27 Jun 2017 15:03:22: 10000000 INFO @ Tue, 27 Jun 2017 15:03:24: 10000000 INFO @ Tue, 27 Jun 2017 15:03:24: 10000000 INFO @ Tue, 27 Jun 2017 15:03:28: 11000000 INFO @ Tue, 27 Jun 2017 15:03:30: 11000000 INFO @ Tue, 27 Jun 2017 15:03:30: 11000000 INFO @ Tue, 27 Jun 2017 15:03:35: 12000000 INFO @ Tue, 27 Jun 2017 15:03:37: 12000000 INFO @ Tue, 27 Jun 2017 15:03:37: 12000000 INFO @ Tue, 27 Jun 2017 15:03:41: 13000000 INFO @ Tue, 27 Jun 2017 15:03:44: 13000000 INFO @ Tue, 27 Jun 2017 15:03:44: 13000000 INFO @ Tue, 27 Jun 2017 15:03:48: 14000000 INFO @ Tue, 27 Jun 2017 15:03:50: 14000000 INFO @ Tue, 27 Jun 2017 15:03:50: 14000000 INFO @ Tue, 27 Jun 2017 15:03:54: 15000000 INFO @ Tue, 27 Jun 2017 15:03:57: 15000000 INFO @ Tue, 27 Jun 2017 15:03:57: 15000000 INFO @ Tue, 27 Jun 2017 15:04:01: 16000000 INFO @ Tue, 27 Jun 2017 15:04:03: 16000000 INFO @ Tue, 27 Jun 2017 15:04:03: 16000000 INFO @ Tue, 27 Jun 2017 15:04:07: 17000000 INFO @ Tue, 27 Jun 2017 15:04:10: 17000000 INFO @ Tue, 27 Jun 2017 15:04:10: 17000000 INFO @ Tue, 27 Jun 2017 15:04:14: 18000000 INFO @ Tue, 27 Jun 2017 15:04:17: 18000000 INFO @ Tue, 27 Jun 2017 15:04:17: 18000000 INFO @ Tue, 27 Jun 2017 15:04:20: 19000000 INFO @ Tue, 27 Jun 2017 15:04:23: 19000000 INFO @ Tue, 27 Jun 2017 15:04:23: 19000000 INFO @ Tue, 27 Jun 2017 15:04:27: 20000000 INFO @ Tue, 27 Jun 2017 15:04:30: 20000000 INFO @ Tue, 27 Jun 2017 15:04:30: 20000000 INFO @ Tue, 27 Jun 2017 15:04:33: 21000000 INFO @ Tue, 27 Jun 2017 15:04:37: 21000000 INFO @ Tue, 27 Jun 2017 15:04:37: 21000000 INFO @ Tue, 27 Jun 2017 15:04:40: 22000000 INFO @ Tue, 27 Jun 2017 15:04:43: 22000000 INFO @ Tue, 27 Jun 2017 15:04:43: 22000000 INFO @ Tue, 27 Jun 2017 15:04:46: 23000000 INFO @ Tue, 27 Jun 2017 15:04:50: 23000000 INFO @ Tue, 27 Jun 2017 15:04:50: 23000000 INFO @ Tue, 27 Jun 2017 15:04:50: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:04:50: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:04:50: #1 total tags in treatment: 23617456 INFO @ Tue, 27 Jun 2017 15:04:50: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:04:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:04:51: #1 tags after filtering in treatment: 23617456 INFO @ Tue, 27 Jun 2017 15:04:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:04:51: #1 finished! INFO @ Tue, 27 Jun 2017 15:04:51: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:04:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:04:52: #2 number of paired peaks: 76 WARNING @ Tue, 27 Jun 2017 15:04:52: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:04:52: Process for pairing-model is terminated! cat: SRX030961.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX030961.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030961.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030961.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:04:54: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:04:54: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:04:54: #1 total tags in treatment: 23617456 INFO @ Tue, 27 Jun 2017 15:04:54: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:04:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:04:54: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:04:54: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:04:54: #1 total tags in treatment: 23617456 INFO @ Tue, 27 Jun 2017 15:04:54: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:04:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:04:55: #1 tags after filtering in treatment: 23617456 INFO @ Tue, 27 Jun 2017 15:04:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:04:55: #1 finished! INFO @ Tue, 27 Jun 2017 15:04:55: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:04:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:04:55: #1 tags after filtering in treatment: 23617456 INFO @ Tue, 27 Jun 2017 15:04:55: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:04:55: #1 finished! INFO @ Tue, 27 Jun 2017 15:04:55: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:04:55: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:04:56: #2 number of paired peaks: 76 WARNING @ Tue, 27 Jun 2017 15:04:56: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:04:56: Process for pairing-model is terminated! cat: SRX030961.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX030961.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030961.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030961.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:04:56: #2 number of paired peaks: 76 WARNING @ Tue, 27 Jun 2017 15:04:56: Too few paired peaks (76) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:04:56: Process for pairing-model is terminated! cat: SRX030961.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX030961.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030961.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX030961.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。