Job ID = 9158056 sra ファイルのダウンロード中... Completed: 423247K bytes transferred in 6 seconds (556832K bits/sec), in 1 file, 2 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 27253273 spots for /home/okishinya/chipatlas/results/dm3/SRX025477/SRR063876.sra Written 27253273 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:05:44 27253273 reads; of these: 27253273 (100.00%) were unpaired; of these: 2083090 (7.64%) aligned 0 times 21510241 (78.93%) aligned exactly 1 time 3659942 (13.43%) aligned >1 times 92.36% overall alignment rate Time searching: 00:05:44 Overall time: 00:05:44 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 8 files... [bam_rmdupse_core] 6907445 / 25170183 = 0.2744 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 14:54:53: # Command line: callpeak -t SRX025477.bam -f BAM -g dm -n SRX025477.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX025477.10 # format = BAM # ChIP-seq file = ['SRX025477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:54:53: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:54:53: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:54:53: # Command line: callpeak -t SRX025477.bam -f BAM -g dm -n SRX025477.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX025477.05 # format = BAM # ChIP-seq file = ['SRX025477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:54:53: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:54:53: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:54:53: # Command line: callpeak -t SRX025477.bam -f BAM -g dm -n SRX025477.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX025477.20 # format = BAM # ChIP-seq file = ['SRX025477.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 14:54:53: #1 read tag files... INFO @ Tue, 27 Jun 2017 14:54:53: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 14:55:00: 1000000 INFO @ Tue, 27 Jun 2017 14:55:00: 1000000 INFO @ Tue, 27 Jun 2017 14:55:00: 1000000 INFO @ Tue, 27 Jun 2017 14:55:07: 2000000 INFO @ Tue, 27 Jun 2017 14:55:08: 2000000 INFO @ Tue, 27 Jun 2017 14:55:08: 2000000 INFO @ Tue, 27 Jun 2017 14:55:13: 3000000 INFO @ Tue, 27 Jun 2017 14:55:15: 3000000 INFO @ Tue, 27 Jun 2017 14:55:15: 3000000 INFO @ Tue, 27 Jun 2017 14:55:19: 4000000 INFO @ Tue, 27 Jun 2017 14:55:22: 4000000 INFO @ Tue, 27 Jun 2017 14:55:22: 4000000 INFO @ Tue, 27 Jun 2017 14:55:26: 5000000 INFO @ Tue, 27 Jun 2017 14:55:30: 5000000 INFO @ Tue, 27 Jun 2017 14:55:30: 5000000 INFO @ Tue, 27 Jun 2017 14:55:32: 6000000 INFO @ Tue, 27 Jun 2017 14:55:37: 6000000 INFO @ Tue, 27 Jun 2017 14:55:37: 6000000 INFO @ Tue, 27 Jun 2017 14:55:39: 7000000 INFO @ Tue, 27 Jun 2017 14:55:44: 7000000 INFO @ Tue, 27 Jun 2017 14:55:44: 7000000 INFO @ Tue, 27 Jun 2017 14:55:45: 8000000 INFO @ Tue, 27 Jun 2017 14:55:50: 8000000 INFO @ Tue, 27 Jun 2017 14:55:50: 8000000 INFO @ Tue, 27 Jun 2017 14:55:51: 9000000 INFO @ Tue, 27 Jun 2017 14:55:57: 9000000 INFO @ Tue, 27 Jun 2017 14:55:57: 9000000 INFO @ Tue, 27 Jun 2017 14:55:58: 10000000 INFO @ Tue, 27 Jun 2017 14:56:04: 10000000 INFO @ Tue, 27 Jun 2017 14:56:05: 10000000 INFO @ Tue, 27 Jun 2017 14:56:06: 11000000 INFO @ Tue, 27 Jun 2017 14:56:11: 11000000 INFO @ Tue, 27 Jun 2017 14:56:12: 11000000 INFO @ Tue, 27 Jun 2017 14:56:14: 12000000 INFO @ Tue, 27 Jun 2017 14:56:18: 12000000 INFO @ Tue, 27 Jun 2017 14:56:19: 12000000 INFO @ Tue, 27 Jun 2017 14:56:21: 13000000 INFO @ Tue, 27 Jun 2017 14:56:25: 13000000 INFO @ Tue, 27 Jun 2017 14:56:26: 13000000 INFO @ Tue, 27 Jun 2017 14:56:29: 14000000 INFO @ Tue, 27 Jun 2017 14:56:32: 14000000 INFO @ Tue, 27 Jun 2017 14:56:34: 14000000 INFO @ Tue, 27 Jun 2017 14:56:37: 15000000 INFO @ Tue, 27 Jun 2017 14:56:39: 15000000 INFO @ Tue, 27 Jun 2017 14:56:41: 15000000 INFO @ Tue, 27 Jun 2017 14:56:44: 16000000 INFO @ Tue, 27 Jun 2017 14:56:46: 16000000 INFO @ Tue, 27 Jun 2017 14:56:48: 16000000 INFO @ Tue, 27 Jun 2017 14:56:51: 17000000 INFO @ Tue, 27 Jun 2017 14:56:52: 17000000 INFO @ Tue, 27 Jun 2017 14:56:54: 17000000 INFO @ Tue, 27 Jun 2017 14:56:58: 18000000 INFO @ Tue, 27 Jun 2017 14:56:58: 18000000 INFO @ Tue, 27 Jun 2017 14:57:00: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 14:57:00: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 14:57:00: #1 total tags in treatment: 18262738 INFO @ Tue, 27 Jun 2017 14:57:00: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:57:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:57:00: #1 tags after filtering in treatment: 18262738 INFO @ Tue, 27 Jun 2017 14:57:00: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:57:00: #1 finished! INFO @ Tue, 27 Jun 2017 14:57:00: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:57:00: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:57:00: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 14:57:00: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 14:57:00: #1 total tags in treatment: 18262738 INFO @ Tue, 27 Jun 2017 14:57:00: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:57:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:57:01: #1 tags after filtering in treatment: 18262738 INFO @ Tue, 27 Jun 2017 14:57:01: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:57:01: #1 finished! INFO @ Tue, 27 Jun 2017 14:57:01: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:57:01: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:57:01: 18000000 INFO @ Tue, 27 Jun 2017 14:57:01: #2 number of paired peaks: 88 WARNING @ Tue, 27 Jun 2017 14:57:01: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:57:01: Process for pairing-model is terminated! cat: SRX025477.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX025477.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX025477.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX025477.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 14:57:02: #2 number of paired peaks: 88 WARNING @ Tue, 27 Jun 2017 14:57:02: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:57:02: Process for pairing-model is terminated! cat: SRX025477.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX025477.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX025477.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX025477.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 14:57:03: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 14:57:03: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 14:57:03: #1 total tags in treatment: 18262738 INFO @ Tue, 27 Jun 2017 14:57:03: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 14:57:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 14:57:03: #1 tags after filtering in treatment: 18262738 INFO @ Tue, 27 Jun 2017 14:57:03: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 14:57:03: #1 finished! INFO @ Tue, 27 Jun 2017 14:57:03: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 14:57:03: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 14:57:04: #2 number of paired peaks: 88 WARNING @ Tue, 27 Jun 2017 14:57:04: Too few paired peaks (88) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 14:57:04: Process for pairing-model is terminated! cat: SRX025477.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX025477.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX025477.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX025477.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。