Job ID = 6527470 SRX = SRX017855 Genome = dm3 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... 2020-06-29T13:04:39 prefetch.2.10.7: 1) Downloading 'SRR038290'... 2020-06-29T13:04:39 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:06:00 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:06:01 prefetch.2.10.7: 'SRR038290' is valid 2020-06-29T13:06:01 prefetch.2.10.7: 1) 'SRR038290' was downloaded successfully Read 11791287 spots for SRR038290/SRR038290.sra Written 11791287 spots for SRR038290/SRR038290.sra 2020-06-29T13:06:52 prefetch.2.10.7: 1) Downloading 'SRR038291'... 2020-06-29T13:06:52 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:07:51 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:07:52 prefetch.2.10.7: 'SRR038291' is valid 2020-06-29T13:07:52 prefetch.2.10.7: 1) 'SRR038291' was downloaded successfully Read 7161626 spots for SRR038291/SRR038291.sra Written 7161626 spots for SRR038291/SRR038291.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:54 18952913 reads; of these: 18952913 (100.00%) were unpaired; of these: 15278733 (80.61%) aligned 0 times 2760198 (14.56%) aligned exactly 1 time 913982 (4.82%) aligned >1 times 19.39% overall alignment rate Time searching: 00:01:54 Overall time: 00:01:54 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 524334 / 3674180 = 0.1427 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:14:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:14:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:14:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:14:27: 1000000 INFO @ Mon, 29 Jun 2020 22:14:32: 2000000 INFO @ Mon, 29 Jun 2020 22:14:37: 3000000 INFO @ Mon, 29 Jun 2020 22:14:38: #1 tag size is determined as 27 bps INFO @ Mon, 29 Jun 2020 22:14:38: #1 tag size = 27 INFO @ Mon, 29 Jun 2020 22:14:38: #1 total tags in treatment: 3149846 INFO @ Mon, 29 Jun 2020 22:14:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:14:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:14:38: #1 tags after filtering in treatment: 3149846 INFO @ Mon, 29 Jun 2020 22:14:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:14:38: #1 finished! INFO @ Mon, 29 Jun 2020 22:14:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:14:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:14:38: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:14:38: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:14:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:14:52: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:14:52: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:14:52: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:14:57: 1000000 INFO @ Mon, 29 Jun 2020 22:15:03: 2000000 INFO @ Mon, 29 Jun 2020 22:15:08: 3000000 INFO @ Mon, 29 Jun 2020 22:15:09: #1 tag size is determined as 27 bps INFO @ Mon, 29 Jun 2020 22:15:09: #1 tag size = 27 INFO @ Mon, 29 Jun 2020 22:15:09: #1 total tags in treatment: 3149846 INFO @ Mon, 29 Jun 2020 22:15:09: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:15:09: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:15:09: #1 tags after filtering in treatment: 3149846 INFO @ Mon, 29 Jun 2020 22:15:09: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:15:09: #1 finished! INFO @ Mon, 29 Jun 2020 22:15:09: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:15:09: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:15:09: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:15:09: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:15:09: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Mon, 29 Jun 2020 22:15:22: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Mon, 29 Jun 2020 22:15:22: #1 read tag files... INFO @ Mon, 29 Jun 2020 22:15:22: #1 read treatment tags... INFO @ Mon, 29 Jun 2020 22:15:27: 1000000 INFO @ Mon, 29 Jun 2020 22:15:32: 2000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Mon, 29 Jun 2020 22:15:37: 3000000 INFO @ Mon, 29 Jun 2020 22:15:38: #1 tag size is determined as 27 bps INFO @ Mon, 29 Jun 2020 22:15:38: #1 tag size = 27 INFO @ Mon, 29 Jun 2020 22:15:38: #1 total tags in treatment: 3149846 INFO @ Mon, 29 Jun 2020 22:15:38: #1 user defined the maximum tags... INFO @ Mon, 29 Jun 2020 22:15:38: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Mon, 29 Jun 2020 22:15:38: #1 tags after filtering in treatment: 3149846 INFO @ Mon, 29 Jun 2020 22:15:38: #1 Redundant rate of treatment: 0.00 INFO @ Mon, 29 Jun 2020 22:15:38: #1 finished! INFO @ Mon, 29 Jun 2020 22:15:38: #2 Build Peak Model... INFO @ Mon, 29 Jun 2020 22:15:38: #2 looking for paired plus/minus strand peaks... INFO @ Mon, 29 Jun 2020 22:15:38: #2 number of paired peaks: 32 WARNING @ Mon, 29 Jun 2020 22:15:38: Too few paired peaks (32) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Mon, 29 Jun 2020 22:15:38: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/SRX017855/SRX017855.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。