Job ID = 9158042 sra ファイルのダウンロード中... Completed: 1027997K bytes transferred in 11 seconds (733591K bits/sec), in 3 files, 4 directories. sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 13213445 spots for /home/okishinya/chipatlas/results/dm3/SRX016170/SRR034739.sra Written 13213445 spots total Written 14604881 spots for /home/okishinya/chipatlas/results/dm3/SRX016170/SRR034740.sra Written 14604881 spots total Written 15544574 spots for /home/okishinya/chipatlas/results/dm3/SRX016170/SRR034738.sra Written 15544574 spots total fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:10:46 43362900 reads; of these: 43362900 (100.00%) were unpaired; of these: 3181965 (7.34%) aligned 0 times 33505526 (77.27%) aligned exactly 1 time 6675409 (15.39%) aligned >1 times 92.66% overall alignment rate Time searching: 00:10:47 Overall time: 00:10:47 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdupse_core] 14278035 / 40180935 = 0.3553 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 27 Jun 2017 15:01:12: # Command line: callpeak -t SRX016170.bam -f BAM -g dm -n SRX016170.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX016170.05 # format = BAM # ChIP-seq file = ['SRX016170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:01:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:01:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:01:12: # Command line: callpeak -t SRX016170.bam -f BAM -g dm -n SRX016170.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX016170.20 # format = BAM # ChIP-seq file = ['SRX016170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:01:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:01:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:01:12: # Command line: callpeak -t SRX016170.bam -f BAM -g dm -n SRX016170.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX016170.10 # format = BAM # ChIP-seq file = ['SRX016170.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 27 Jun 2017 15:01:12: #1 read tag files... INFO @ Tue, 27 Jun 2017 15:01:12: #1 read treatment tags... INFO @ Tue, 27 Jun 2017 15:01:18: 1000000 INFO @ Tue, 27 Jun 2017 15:01:18: 1000000 INFO @ Tue, 27 Jun 2017 15:01:18: 1000000 INFO @ Tue, 27 Jun 2017 15:01:23: 2000000 INFO @ Tue, 27 Jun 2017 15:01:24: 2000000 INFO @ Tue, 27 Jun 2017 15:01:24: 2000000 INFO @ Tue, 27 Jun 2017 15:01:29: 3000000 INFO @ Tue, 27 Jun 2017 15:01:30: 3000000 INFO @ Tue, 27 Jun 2017 15:01:30: 3000000 INFO @ Tue, 27 Jun 2017 15:01:35: 4000000 INFO @ Tue, 27 Jun 2017 15:01:36: 4000000 INFO @ Tue, 27 Jun 2017 15:01:36: 4000000 INFO @ Tue, 27 Jun 2017 15:01:41: 5000000 INFO @ Tue, 27 Jun 2017 15:01:42: 5000000 INFO @ Tue, 27 Jun 2017 15:01:42: 5000000 INFO @ Tue, 27 Jun 2017 15:01:47: 6000000 INFO @ Tue, 27 Jun 2017 15:01:48: 6000000 INFO @ Tue, 27 Jun 2017 15:01:48: 6000000 INFO @ Tue, 27 Jun 2017 15:01:53: 7000000 INFO @ Tue, 27 Jun 2017 15:01:54: 7000000 INFO @ Tue, 27 Jun 2017 15:01:54: 7000000 INFO @ Tue, 27 Jun 2017 15:01:59: 8000000 INFO @ Tue, 27 Jun 2017 15:02:00: 8000000 INFO @ Tue, 27 Jun 2017 15:02:00: 8000000 INFO @ Tue, 27 Jun 2017 15:02:05: 9000000 INFO @ Tue, 27 Jun 2017 15:02:06: 9000000 INFO @ Tue, 27 Jun 2017 15:02:06: 9000000 INFO @ Tue, 27 Jun 2017 15:02:11: 10000000 INFO @ Tue, 27 Jun 2017 15:02:12: 10000000 INFO @ Tue, 27 Jun 2017 15:02:12: 10000000 INFO @ Tue, 27 Jun 2017 15:02:17: 11000000 INFO @ Tue, 27 Jun 2017 15:02:18: 11000000 INFO @ Tue, 27 Jun 2017 15:02:19: 11000000 INFO @ Tue, 27 Jun 2017 15:02:23: 12000000 INFO @ Tue, 27 Jun 2017 15:02:25: 12000000 INFO @ Tue, 27 Jun 2017 15:02:25: 12000000 INFO @ Tue, 27 Jun 2017 15:02:29: 13000000 INFO @ Tue, 27 Jun 2017 15:02:31: 13000000 INFO @ Tue, 27 Jun 2017 15:02:31: 13000000 INFO @ Tue, 27 Jun 2017 15:02:35: 14000000 INFO @ Tue, 27 Jun 2017 15:02:37: 14000000 INFO @ Tue, 27 Jun 2017 15:02:37: 14000000 INFO @ Tue, 27 Jun 2017 15:02:41: 15000000 INFO @ Tue, 27 Jun 2017 15:02:43: 15000000 INFO @ Tue, 27 Jun 2017 15:02:43: 15000000 INFO @ Tue, 27 Jun 2017 15:02:47: 16000000 INFO @ Tue, 27 Jun 2017 15:02:49: 16000000 INFO @ Tue, 27 Jun 2017 15:02:49: 16000000 INFO @ Tue, 27 Jun 2017 15:02:53: 17000000 INFO @ Tue, 27 Jun 2017 15:02:55: 17000000 INFO @ Tue, 27 Jun 2017 15:02:55: 17000000 INFO @ Tue, 27 Jun 2017 15:02:58: 18000000 INFO @ Tue, 27 Jun 2017 15:03:01: 18000000 INFO @ Tue, 27 Jun 2017 15:03:01: 18000000 INFO @ Tue, 27 Jun 2017 15:03:04: 19000000 INFO @ Tue, 27 Jun 2017 15:03:07: 19000000 INFO @ Tue, 27 Jun 2017 15:03:07: 19000000 INFO @ Tue, 27 Jun 2017 15:03:10: 20000000 INFO @ Tue, 27 Jun 2017 15:03:13: 20000000 INFO @ Tue, 27 Jun 2017 15:03:14: 20000000 INFO @ Tue, 27 Jun 2017 15:03:16: 21000000 INFO @ Tue, 27 Jun 2017 15:03:20: 21000000 INFO @ Tue, 27 Jun 2017 15:03:20: 21000000 INFO @ Tue, 27 Jun 2017 15:03:22: 22000000 INFO @ Tue, 27 Jun 2017 15:03:26: 22000000 INFO @ Tue, 27 Jun 2017 15:03:26: 22000000 INFO @ Tue, 27 Jun 2017 15:03:28: 23000000 INFO @ Tue, 27 Jun 2017 15:03:32: 23000000 INFO @ Tue, 27 Jun 2017 15:03:32: 23000000 INFO @ Tue, 27 Jun 2017 15:03:35: 24000000 INFO @ Tue, 27 Jun 2017 15:03:38: 24000000 INFO @ Tue, 27 Jun 2017 15:03:38: 24000000 INFO @ Tue, 27 Jun 2017 15:03:41: 25000000 INFO @ Tue, 27 Jun 2017 15:03:44: 25000000 INFO @ Tue, 27 Jun 2017 15:03:44: 25000000 INFO @ Tue, 27 Jun 2017 15:03:46: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:03:46: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:03:46: #1 total tags in treatment: 25902900 INFO @ Tue, 27 Jun 2017 15:03:46: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:03:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:03:47: #1 tags after filtering in treatment: 25902900 INFO @ Tue, 27 Jun 2017 15:03:47: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:03:47: #1 finished! INFO @ Tue, 27 Jun 2017 15:03:47: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:03:47: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:03:48: #2 number of paired peaks: 15 WARNING @ Tue, 27 Jun 2017 15:03:48: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:03:48: Process for pairing-model is terminated! cat: SRX016170.05_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX016170.05_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX016170.05_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX016170.05_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:03:50: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:03:50: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:03:50: #1 total tags in treatment: 25902900 INFO @ Tue, 27 Jun 2017 15:03:50: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:03:50: #1 tag size is determined as 36 bps INFO @ Tue, 27 Jun 2017 15:03:50: #1 tag size = 36 INFO @ Tue, 27 Jun 2017 15:03:50: #1 total tags in treatment: 25902900 INFO @ Tue, 27 Jun 2017 15:03:50: #1 user defined the maximum tags... INFO @ Tue, 27 Jun 2017 15:03:50: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 27 Jun 2017 15:03:50: #1 tags after filtering in treatment: 25902900 INFO @ Tue, 27 Jun 2017 15:03:50: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:03:50: #1 finished! INFO @ Tue, 27 Jun 2017 15:03:50: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:03:50: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:03:51: #1 tags after filtering in treatment: 25902900 INFO @ Tue, 27 Jun 2017 15:03:51: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 27 Jun 2017 15:03:51: #1 finished! INFO @ Tue, 27 Jun 2017 15:03:51: #2 Build Peak Model... INFO @ Tue, 27 Jun 2017 15:03:51: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 27 Jun 2017 15:03:52: #2 number of paired peaks: 15 WARNING @ Tue, 27 Jun 2017 15:03:52: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:03:52: Process for pairing-model is terminated! cat: SRX016170.10_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX016170.10_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX016170.10_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX016170.10_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling INFO @ Tue, 27 Jun 2017 15:03:52: #2 number of paired peaks: 15 WARNING @ Tue, 27 Jun 2017 15:03:52: Too few paired peaks (15) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 27 Jun 2017 15:03:52: Process for pairing-model is terminated! cat: SRX016170.20_peaks.narrowPeak: そのようなファイルやディレクトリはありません pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove `SRX016170.20_model.r': そのようなファイルやディレクトリはありません rm: cannot remove `SRX016170.20_*.xls': そのようなファイルやディレクトリはありません rm: cannot remove `SRX016170.20_peaks.narrowPeak': そのようなファイルやディレクトリはありません CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。