Job ID = 2161075 sra ファイルのダウンロード中... Completed: 116227K bytes transferred in 4 seconds (216056K bits/sec), in 1 file, 2 directories. % Total % Received % Xferd Average Speed Time Time Time Current Dload Upload Total Spent Left Speed 0 0 0 0 0 0 0 0 --:--:-- --:--:-- --:--:-- 0 100 14916 0 14916 0 0 21475 0 --:--:-- --:--:-- --:--:-- 29595 100 35135 0 35135 0 0 50525 0 --:--:-- --:--:-- --:--:-- 69574 sra ファイルのダウンロードが完了しました。 Read layout: SINGLE fastq に変換中... Written 4682572 spots for /home/okishinya/chipatlas/results/dm3/SRX013009/SRR030275.sra Written 4682572 spots total rm: cannot remove `[DSE]RR*.fastq': そのようなファイルやディレクトリはありません fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:07 4682572 reads; of these: 4682572 (100.00%) were unpaired; of these: 584583 (12.48%) aligned 0 times 3417405 (72.98%) aligned exactly 1 time 680584 (14.53%) aligned >1 times 87.52% overall alignment rate Time searching: 00:01:07 Overall time: 00:01:07 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 152297 / 4097989 = 0.0372 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Tue, 21 Apr 2015 11:55:42: # Command line: callpeak -t SRX013009.bam -f BAM -g dm -n SRX013009.10 -q 1e-10 # ARGUMENTS LIST: # name = SRX013009.10 # format = BAM # ChIP-seq file = ['SRX013009.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:55:42: # Command line: callpeak -t SRX013009.bam -f BAM -g dm -n SRX013009.20 -q 1e-20 # ARGUMENTS LIST: # name = SRX013009.20 # format = BAM # ChIP-seq file = ['SRX013009.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:55:42: # Command line: callpeak -t SRX013009.bam -f BAM -g dm -n SRX013009.05 -q 1e-05 # ARGUMENTS LIST: # name = SRX013009.05 # format = BAM # ChIP-seq file = ['SRX013009.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off INFO @ Tue, 21 Apr 2015 11:55:42: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:55:42: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:55:42: #1 read tag files... INFO @ Tue, 21 Apr 2015 11:55:42: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:55:42: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:55:42: #1 read treatment tags... INFO @ Tue, 21 Apr 2015 11:55:48: 1000000 INFO @ Tue, 21 Apr 2015 11:55:48: 1000000 INFO @ Tue, 21 Apr 2015 11:55:48: 1000000 INFO @ Tue, 21 Apr 2015 11:55:53: 2000000 INFO @ Tue, 21 Apr 2015 11:55:53: 2000000 INFO @ Tue, 21 Apr 2015 11:55:53: 2000000 INFO @ Tue, 21 Apr 2015 11:55:58: 3000000 INFO @ Tue, 21 Apr 2015 11:55:58: 3000000 INFO @ Tue, 21 Apr 2015 11:55:58: 3000000 INFO @ Tue, 21 Apr 2015 11:56:03: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:56:03: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:56:03: #1 total tags in treatment: 3945692 INFO @ Tue, 21 Apr 2015 11:56:03: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:56:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:56:03: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:56:03: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:56:03: #1 total tags in treatment: 3945692 INFO @ Tue, 21 Apr 2015 11:56:03: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:56:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:56:03: #1 tag size is determined as 36 bps INFO @ Tue, 21 Apr 2015 11:56:03: #1 tag size = 36 INFO @ Tue, 21 Apr 2015 11:56:03: #1 total tags in treatment: 3945692 INFO @ Tue, 21 Apr 2015 11:56:03: #1 user defined the maximum tags... INFO @ Tue, 21 Apr 2015 11:56:03: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 21 Apr 2015 11:56:04: #1 tags after filtering in treatment: 3945496 INFO @ Tue, 21 Apr 2015 11:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:56:04: #1 finished! INFO @ Tue, 21 Apr 2015 11:56:04: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:56:04: #1 tags after filtering in treatment: 3945496 INFO @ Tue, 21 Apr 2015 11:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:56:04: #1 finished! INFO @ Tue, 21 Apr 2015 11:56:04: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:56:04: #1 tags after filtering in treatment: 3945496 INFO @ Tue, 21 Apr 2015 11:56:04: #1 Redundant rate of treatment: 0.00 INFO @ Tue, 21 Apr 2015 11:56:04: #1 finished! INFO @ Tue, 21 Apr 2015 11:56:04: #2 Build Peak Model... INFO @ Tue, 21 Apr 2015 11:56:04: #2 number of paired peaks: 747 WARNING @ Tue, 21 Apr 2015 11:56:04: Fewer paired peaks (747) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 747 pairs to build model! INFO @ Tue, 21 Apr 2015 11:56:04: start model_add_line... INFO @ Tue, 21 Apr 2015 11:56:04: #2 number of paired peaks: 747 WARNING @ Tue, 21 Apr 2015 11:56:04: Fewer paired peaks (747) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 747 pairs to build model! INFO @ Tue, 21 Apr 2015 11:56:04: start model_add_line... INFO @ Tue, 21 Apr 2015 11:56:05: #2 number of paired peaks: 747 WARNING @ Tue, 21 Apr 2015 11:56:05: Fewer paired peaks (747) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 747 pairs to build model! INFO @ Tue, 21 Apr 2015 11:56:05: start model_add_line... INFO @ Tue, 21 Apr 2015 11:56:06: start X-correlation... INFO @ Tue, 21 Apr 2015 11:56:06: end of X-cor INFO @ Tue, 21 Apr 2015 11:56:06: #2 finished! INFO @ Tue, 21 Apr 2015 11:56:06: #2 predicted fragment length is 145 bps INFO @ Tue, 21 Apr 2015 11:56:06: #2 alternative fragment length(s) may be 145 bps INFO @ Tue, 21 Apr 2015 11:56:06: #2.2 Generate R script for model : SRX013009.10_model.r INFO @ Tue, 21 Apr 2015 11:56:06: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:56:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:56:06: start X-correlation... INFO @ Tue, 21 Apr 2015 11:56:06: end of X-cor INFO @ Tue, 21 Apr 2015 11:56:06: #2 finished! INFO @ Tue, 21 Apr 2015 11:56:06: #2 predicted fragment length is 145 bps INFO @ Tue, 21 Apr 2015 11:56:06: #2 alternative fragment length(s) may be 145 bps INFO @ Tue, 21 Apr 2015 11:56:06: #2.2 Generate R script for model : SRX013009.20_model.r INFO @ Tue, 21 Apr 2015 11:56:06: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:56:06: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:56:07: start X-correlation... INFO @ Tue, 21 Apr 2015 11:56:07: end of X-cor INFO @ Tue, 21 Apr 2015 11:56:07: #2 finished! INFO @ Tue, 21 Apr 2015 11:56:07: #2 predicted fragment length is 145 bps INFO @ Tue, 21 Apr 2015 11:56:07: #2 alternative fragment length(s) may be 145 bps INFO @ Tue, 21 Apr 2015 11:56:07: #2.2 Generate R script for model : SRX013009.05_model.r INFO @ Tue, 21 Apr 2015 11:56:07: #3 Call peaks... INFO @ Tue, 21 Apr 2015 11:56:07: #3 Pre-compute pvalue-qvalue table... INFO @ Tue, 21 Apr 2015 11:56:29: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:56:29: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:56:29: #3 Call peaks for each chromosome... INFO @ Tue, 21 Apr 2015 11:56:45: #4 Write output xls file... SRX013009.10_peaks.xls INFO @ Tue, 21 Apr 2015 11:56:45: #4 Write peak in narrowPeak format file... SRX013009.10_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:56:45: #4 Write summits bed file... SRX013009.10_summits.bed INFO @ Tue, 21 Apr 2015 11:56:45: Done! pass1 - making usageList (9 chroms): 1 millis pass2 - checking and writing primary data (749 records, 4 fields): 3 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 11:56:46: #4 Write output xls file... SRX013009.20_peaks.xls INFO @ Tue, 21 Apr 2015 11:56:46: #4 Write peak in narrowPeak format file... SRX013009.20_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:56:46: #4 Write summits bed file... SRX013009.20_summits.bed INFO @ Tue, 21 Apr 2015 11:56:46: Done! pass1 - making usageList (8 chroms): 1 millis pass2 - checking and writing primary data (131 records, 4 fields): 1 millis CompletedMACS2peakCalling INFO @ Tue, 21 Apr 2015 11:56:48: #4 Write output xls file... SRX013009.05_peaks.xls INFO @ Tue, 21 Apr 2015 11:56:48: #4 Write peak in narrowPeak format file... SRX013009.05_peaks.narrowPeak INFO @ Tue, 21 Apr 2015 11:56:48: #4 Write summits bed file... SRX013009.05_summits.bed INFO @ Tue, 21 Apr 2015 11:56:48: Done! pass1 - making usageList (13 chroms): 1 millis pass2 - checking and writing primary data (2770 records, 4 fields): 5 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。