Job ID = 16439819 SRX = ERX5970266 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T07:14:39 prefetch.2.10.7: 1) Downloading 'ERR6338589'... 2022-08-02T07:14:39 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T07:17:48 prefetch.2.10.7: HTTPS download succeed 2022-08-02T07:17:48 prefetch.2.10.7: 1) 'ERR6338589' was downloaded successfully 2022-08-02T07:17:48 prefetch.2.10.7: 'ERR6338589' has 0 unresolved dependencies Read 27106931 spots for ERR6338589/ERR6338589.sra Written 27106931 spots for ERR6338589/ERR6338589.sra fastq に変換しました。 bowtie でマッピング中... Your job 16440957 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:01 Multiseed full-index search: 01:47:24 27106931 reads; of these: 27106931 (100.00%) were paired; of these: 3749633 (13.83%) aligned concordantly 0 times 18644466 (68.78%) aligned concordantly exactly 1 time 4712832 (17.39%) aligned concordantly >1 times ---- 3749633 pairs aligned concordantly 0 times; of these: 2105477 (56.15%) aligned discordantly 1 time ---- 1644156 pairs aligned 0 times concordantly or discordantly; of these: 3288312 mates make up the pairs; of these: 1641239 (49.91%) aligned 0 times 612704 (18.63%) aligned exactly 1 time 1034369 (31.46%) aligned >1 times 96.97% overall alignment rate Time searching: 01:47:25 Overall time: 01:47:25 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1065885 / 17299189 = 0.0616 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:40:43: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:40:43: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:40:43: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:40:52: 1000000 INFO @ Tue, 02 Aug 2022 18:41:00: 2000000 INFO @ Tue, 02 Aug 2022 18:41:08: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:41:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:41:12: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:41:12: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:41:17: 4000000 INFO @ Tue, 02 Aug 2022 18:41:21: 1000000 INFO @ Tue, 02 Aug 2022 18:41:27: 5000000 INFO @ Tue, 02 Aug 2022 18:41:30: 2000000 INFO @ Tue, 02 Aug 2022 18:41:36: 6000000 INFO @ Tue, 02 Aug 2022 18:41:40: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 18:41:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 18:41:42: #1 read tag files... INFO @ Tue, 02 Aug 2022 18:41:42: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 18:41:45: 7000000 INFO @ Tue, 02 Aug 2022 18:41:49: 4000000 INFO @ Tue, 02 Aug 2022 18:41:49: 1000000 INFO @ Tue, 02 Aug 2022 18:41:55: 8000000 INFO @ Tue, 02 Aug 2022 18:41:56: 2000000 INFO @ Tue, 02 Aug 2022 18:41:58: 5000000 INFO @ Tue, 02 Aug 2022 18:42:03: 3000000 INFO @ Tue, 02 Aug 2022 18:42:05: 9000000 INFO @ Tue, 02 Aug 2022 18:42:08: 6000000 INFO @ Tue, 02 Aug 2022 18:42:10: 4000000 INFO @ Tue, 02 Aug 2022 18:42:14: 10000000 INFO @ Tue, 02 Aug 2022 18:42:17: 7000000 INFO @ Tue, 02 Aug 2022 18:42:17: 5000000 INFO @ Tue, 02 Aug 2022 18:42:24: 11000000 INFO @ Tue, 02 Aug 2022 18:42:24: 6000000 INFO @ Tue, 02 Aug 2022 18:42:27: 8000000 INFO @ Tue, 02 Aug 2022 18:42:31: 7000000 INFO @ Tue, 02 Aug 2022 18:42:33: 12000000 INFO @ Tue, 02 Aug 2022 18:42:36: 9000000 INFO @ Tue, 02 Aug 2022 18:42:38: 8000000 INFO @ Tue, 02 Aug 2022 18:42:43: 13000000 INFO @ Tue, 02 Aug 2022 18:42:46: 10000000 INFO @ Tue, 02 Aug 2022 18:42:47: 9000000 INFO @ Tue, 02 Aug 2022 18:42:52: 14000000 INFO @ Tue, 02 Aug 2022 18:42:55: 10000000 INFO @ Tue, 02 Aug 2022 18:42:55: 11000000 INFO @ Tue, 02 Aug 2022 18:43:02: 15000000 INFO @ Tue, 02 Aug 2022 18:43:03: 11000000 INFO @ Tue, 02 Aug 2022 18:43:04: 12000000 INFO @ Tue, 02 Aug 2022 18:43:11: 16000000 INFO @ Tue, 02 Aug 2022 18:43:12: 12000000 INFO @ Tue, 02 Aug 2022 18:43:14: 13000000 INFO @ Tue, 02 Aug 2022 18:43:21: 17000000 INFO @ Tue, 02 Aug 2022 18:43:22: 13000000 INFO @ Tue, 02 Aug 2022 18:43:23: 14000000 INFO @ Tue, 02 Aug 2022 18:43:30: 18000000 INFO @ Tue, 02 Aug 2022 18:43:32: 14000000 INFO @ Tue, 02 Aug 2022 18:43:33: 15000000 INFO @ Tue, 02 Aug 2022 18:43:40: 19000000 INFO @ Tue, 02 Aug 2022 18:43:42: 15000000 INFO @ Tue, 02 Aug 2022 18:43:43: 16000000 INFO @ Tue, 02 Aug 2022 18:43:50: 20000000 INFO @ Tue, 02 Aug 2022 18:43:52: 16000000 INFO @ Tue, 02 Aug 2022 18:43:53: 17000000 INFO @ Tue, 02 Aug 2022 18:44:00: 21000000 INFO @ Tue, 02 Aug 2022 18:44:01: 17000000 INFO @ Tue, 02 Aug 2022 18:44:02: 18000000 INFO @ Tue, 02 Aug 2022 18:44:10: 22000000 INFO @ Tue, 02 Aug 2022 18:44:11: 18000000 INFO @ Tue, 02 Aug 2022 18:44:12: 19000000 INFO @ Tue, 02 Aug 2022 18:44:20: 23000000 INFO @ Tue, 02 Aug 2022 18:44:21: 19000000 INFO @ Tue, 02 Aug 2022 18:44:22: 20000000 INFO @ Tue, 02 Aug 2022 18:44:30: 20000000 INFO @ Tue, 02 Aug 2022 18:44:30: 24000000 INFO @ Tue, 02 Aug 2022 18:44:32: 21000000 INFO @ Tue, 02 Aug 2022 18:44:40: 21000000 INFO @ Tue, 02 Aug 2022 18:44:41: 25000000 INFO @ Tue, 02 Aug 2022 18:44:42: 22000000 INFO @ Tue, 02 Aug 2022 18:44:50: 22000000 INFO @ Tue, 02 Aug 2022 18:44:51: 26000000 INFO @ Tue, 02 Aug 2022 18:44:52: 23000000 INFO @ Tue, 02 Aug 2022 18:44:59: 23000000 INFO @ Tue, 02 Aug 2022 18:45:00: 27000000 INFO @ Tue, 02 Aug 2022 18:45:02: 24000000 INFO @ Tue, 02 Aug 2022 18:45:09: 24000000 INFO @ Tue, 02 Aug 2022 18:45:10: 28000000 INFO @ Tue, 02 Aug 2022 18:45:11: 25000000 INFO @ Tue, 02 Aug 2022 18:45:19: 25000000 INFO @ Tue, 02 Aug 2022 18:45:19: 29000000 INFO @ Tue, 02 Aug 2022 18:45:20: 26000000 INFO @ Tue, 02 Aug 2022 18:45:29: 30000000 INFO @ Tue, 02 Aug 2022 18:45:29: 26000000 INFO @ Tue, 02 Aug 2022 18:45:30: 27000000 INFO @ Tue, 02 Aug 2022 18:45:38: 31000000 INFO @ Tue, 02 Aug 2022 18:45:39: 27000000 INFO @ Tue, 02 Aug 2022 18:45:39: 28000000 INFO @ Tue, 02 Aug 2022 18:45:48: 32000000 INFO @ Tue, 02 Aug 2022 18:45:48: 28000000 INFO @ Tue, 02 Aug 2022 18:45:49: 29000000 INFO @ Tue, 02 Aug 2022 18:45:58: 29000000 INFO @ Tue, 02 Aug 2022 18:45:58: 33000000 INFO @ Tue, 02 Aug 2022 18:45:59: 30000000 INFO @ Tue, 02 Aug 2022 18:46:07: 30000000 INFO @ Tue, 02 Aug 2022 18:46:08: 34000000 INFO @ Tue, 02 Aug 2022 18:46:09: 31000000 INFO @ Tue, 02 Aug 2022 18:46:16: 31000000 INFO @ Tue, 02 Aug 2022 18:46:18: 35000000 INFO @ Tue, 02 Aug 2022 18:46:19: 32000000 INFO @ Tue, 02 Aug 2022 18:46:25: 32000000 INFO @ Tue, 02 Aug 2022 18:46:28: 36000000 INFO @ Tue, 02 Aug 2022 18:46:29: 33000000 INFO @ Tue, 02 Aug 2022 18:46:34: 33000000 INFO @ Tue, 02 Aug 2022 18:46:38: 37000000 INFO @ Tue, 02 Aug 2022 18:46:39: 34000000 INFO @ Tue, 02 Aug 2022 18:46:44: 34000000 INFO @ Tue, 02 Aug 2022 18:46:48: 38000000 INFO @ Tue, 02 Aug 2022 18:46:50: 35000000 INFO @ Tue, 02 Aug 2022 18:46:53: 35000000 INFO @ Tue, 02 Aug 2022 18:46:59: 39000000 INFO @ Tue, 02 Aug 2022 18:47:00: 36000000 INFO @ Tue, 02 Aug 2022 18:47:03: 36000000 INFO @ Tue, 02 Aug 2022 18:47:09: 40000000 INFO @ Tue, 02 Aug 2022 18:47:10: 37000000 INFO @ Tue, 02 Aug 2022 18:47:13: 37000000 INFO @ Tue, 02 Aug 2022 18:47:20: 41000000 INFO @ Tue, 02 Aug 2022 18:47:20: 38000000 INFO @ Tue, 02 Aug 2022 18:47:23: 38000000 INFO @ Tue, 02 Aug 2022 18:47:31: 42000000 INFO @ Tue, 02 Aug 2022 18:47:31: 39000000 INFO @ Tue, 02 Aug 2022 18:47:33: 39000000 INFO @ Tue, 02 Aug 2022 18:47:42: 43000000 INFO @ Tue, 02 Aug 2022 18:47:42: 40000000 INFO @ Tue, 02 Aug 2022 18:47:44: 40000000 INFO @ Tue, 02 Aug 2022 18:47:53: 41000000 INFO @ Tue, 02 Aug 2022 18:47:53: 44000000 INFO @ Tue, 02 Aug 2022 18:47:54: 41000000 INFO @ Tue, 02 Aug 2022 18:48:03: 42000000 INFO @ Tue, 02 Aug 2022 18:48:04: 45000000 INFO @ Tue, 02 Aug 2022 18:48:04: 42000000 INFO @ Tue, 02 Aug 2022 18:48:14: 46000000 INFO @ Tue, 02 Aug 2022 18:48:14: 43000000 INFO @ Tue, 02 Aug 2022 18:48:15: 43000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 18:48:25: 47000000 INFO @ Tue, 02 Aug 2022 18:48:26: 44000000 INFO @ Tue, 02 Aug 2022 18:48:26: 44000000 INFO @ Tue, 02 Aug 2022 18:48:36: 45000000 INFO @ Tue, 02 Aug 2022 18:48:36: 48000000 INFO @ Tue, 02 Aug 2022 18:48:36: 45000000 INFO @ Tue, 02 Aug 2022 18:48:46: 46000000 INFO @ Tue, 02 Aug 2022 18:48:46: 49000000 INFO @ Tue, 02 Aug 2022 18:48:47: 46000000 INFO @ Tue, 02 Aug 2022 18:48:55: 47000000 INFO @ Tue, 02 Aug 2022 18:48:57: 50000000 INFO @ Tue, 02 Aug 2022 18:48:57: 47000000 INFO @ Tue, 02 Aug 2022 18:49:02: #1 tag size is determined as 116 bps INFO @ Tue, 02 Aug 2022 18:49:02: #1 tag size = 116 INFO @ Tue, 02 Aug 2022 18:49:02: #1 total tags in treatment: 22378498 INFO @ Tue, 02 Aug 2022 18:49:02: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:49:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:49:02: #1 tags after filtering in treatment: 20738492 INFO @ Tue, 02 Aug 2022 18:49:02: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 18:49:02: #1 finished! INFO @ Tue, 02 Aug 2022 18:49:02: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:49:02: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:49:04: #2 number of paired peaks: 64 WARNING @ Tue, 02 Aug 2022 18:49:04: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:49:04: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:49:05: 48000000 INFO @ Tue, 02 Aug 2022 18:49:07: 48000000 INFO @ Tue, 02 Aug 2022 18:49:15: 49000000 INFO @ Tue, 02 Aug 2022 18:49:17: 49000000 INFO @ Tue, 02 Aug 2022 18:49:25: 50000000 INFO @ Tue, 02 Aug 2022 18:49:27: 50000000 INFO @ Tue, 02 Aug 2022 18:49:30: #1 tag size is determined as 116 bps INFO @ Tue, 02 Aug 2022 18:49:30: #1 tag size = 116 INFO @ Tue, 02 Aug 2022 18:49:30: #1 total tags in treatment: 22378498 INFO @ Tue, 02 Aug 2022 18:49:30: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:49:30: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:49:30: #1 tags after filtering in treatment: 20738492 INFO @ Tue, 02 Aug 2022 18:49:30: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 18:49:30: #1 finished! INFO @ Tue, 02 Aug 2022 18:49:30: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:49:30: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:49:32: #2 number of paired peaks: 64 WARNING @ Tue, 02 Aug 2022 18:49:32: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:49:32: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 3 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 18:49:32: #1 tag size is determined as 116 bps INFO @ Tue, 02 Aug 2022 18:49:32: #1 tag size = 116 INFO @ Tue, 02 Aug 2022 18:49:32: #1 total tags in treatment: 22378498 INFO @ Tue, 02 Aug 2022 18:49:32: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 18:49:32: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 18:49:32: #1 tags after filtering in treatment: 20738492 INFO @ Tue, 02 Aug 2022 18:49:32: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 02 Aug 2022 18:49:32: #1 finished! INFO @ Tue, 02 Aug 2022 18:49:32: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 18:49:32: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 18:49:34: #2 number of paired peaks: 64 WARNING @ Tue, 02 Aug 2022 18:49:34: Too few paired peaks (64) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 18:49:34: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5970266/ERX5970266.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。