Job ID = 16437594 SRX = ERX5847884 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2022-08-02T03:52:12 prefetch.2.10.7: 1) Downloading 'ERR6212894'... 2022-08-02T03:52:12 prefetch.2.10.7: Downloading via HTTPS... 2022-08-02T03:53:14 prefetch.2.10.7: HTTPS download succeed 2022-08-02T03:53:14 prefetch.2.10.7: 1) 'ERR6212894' was downloaded successfully 2022-08-02T03:53:14 prefetch.2.10.7: 'ERR6212894' has 0 unresolved dependencies Read 17656775 spots for ERR6212894/ERR6212894.sra Written 17656775 spots for ERR6212894/ERR6212894.sra fastq に変換しました。 bowtie でマッピング中... Your job 16438021 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:15:34 17656775 reads; of these: 17656775 (100.00%) were paired; of these: 4858730 (27.52%) aligned concordantly 0 times 10368882 (58.72%) aligned concordantly exactly 1 time 2429163 (13.76%) aligned concordantly >1 times ---- 4858730 pairs aligned concordantly 0 times; of these: 22997 (0.47%) aligned discordantly 1 time ---- 4835733 pairs aligned 0 times concordantly or discordantly; of these: 9671466 mates make up the pairs; of these: 7770325 (80.34%) aligned 0 times 1504601 (15.56%) aligned exactly 1 time 396540 (4.10%) aligned >1 times 78.00% overall alignment rate Time searching: 00:15:35 Overall time: 00:15:35 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 12 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 144834 / 12808798 = 0.0113 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:21:01: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:21:01: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:21:01: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:21:07: 1000000 INFO @ Tue, 02 Aug 2022 13:21:12: 2000000 INFO @ Tue, 02 Aug 2022 13:21:17: 3000000 INFO @ Tue, 02 Aug 2022 13:21:23: 4000000 INFO @ Tue, 02 Aug 2022 13:21:28: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:21:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:21:30: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:21:30: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:21:33: 6000000 INFO @ Tue, 02 Aug 2022 13:21:36: 1000000 INFO @ Tue, 02 Aug 2022 13:21:38: 7000000 INFO @ Tue, 02 Aug 2022 13:21:41: 2000000 INFO @ Tue, 02 Aug 2022 13:21:43: 8000000 INFO @ Tue, 02 Aug 2022 13:21:47: 3000000 INFO @ Tue, 02 Aug 2022 13:21:48: 9000000 INFO @ Tue, 02 Aug 2022 13:21:53: 4000000 INFO @ Tue, 02 Aug 2022 13:21:53: 10000000 INFO @ Tue, 02 Aug 2022 13:21:58: 5000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.7/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 02 Aug 2022 13:21:59: 11000000 INFO @ Tue, 02 Aug 2022 13:22:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 02 Aug 2022 13:22:00: #1 read tag files... INFO @ Tue, 02 Aug 2022 13:22:00: #1 read treatment tags... INFO @ Tue, 02 Aug 2022 13:22:03: 6000000 INFO @ Tue, 02 Aug 2022 13:22:04: 12000000 INFO @ Tue, 02 Aug 2022 13:22:06: 1000000 INFO @ Tue, 02 Aug 2022 13:22:08: 7000000 INFO @ Tue, 02 Aug 2022 13:22:09: 13000000 INFO @ Tue, 02 Aug 2022 13:22:11: 2000000 INFO @ Tue, 02 Aug 2022 13:22:13: 8000000 INFO @ Tue, 02 Aug 2022 13:22:14: 14000000 INFO @ Tue, 02 Aug 2022 13:22:17: 3000000 INFO @ Tue, 02 Aug 2022 13:22:18: 9000000 INFO @ Tue, 02 Aug 2022 13:22:20: 15000000 INFO @ Tue, 02 Aug 2022 13:22:23: 4000000 INFO @ Tue, 02 Aug 2022 13:22:24: 10000000 INFO @ Tue, 02 Aug 2022 13:22:25: 16000000 INFO @ Tue, 02 Aug 2022 13:22:28: 5000000 INFO @ Tue, 02 Aug 2022 13:22:29: 11000000 INFO @ Tue, 02 Aug 2022 13:22:31: 17000000 INFO @ Tue, 02 Aug 2022 13:22:34: 6000000 INFO @ Tue, 02 Aug 2022 13:22:34: 12000000 INFO @ Tue, 02 Aug 2022 13:22:36: 18000000 INFO @ Tue, 02 Aug 2022 13:22:39: 7000000 INFO @ Tue, 02 Aug 2022 13:22:39: 13000000 INFO @ Tue, 02 Aug 2022 13:22:42: 19000000 INFO @ Tue, 02 Aug 2022 13:22:44: 8000000 INFO @ Tue, 02 Aug 2022 13:22:45: 14000000 INFO @ Tue, 02 Aug 2022 13:22:47: 20000000 INFO @ Tue, 02 Aug 2022 13:22:49: 9000000 INFO @ Tue, 02 Aug 2022 13:22:50: 15000000 INFO @ Tue, 02 Aug 2022 13:22:53: 21000000 INFO @ Tue, 02 Aug 2022 13:22:54: 10000000 INFO @ Tue, 02 Aug 2022 13:22:55: 16000000 INFO @ Tue, 02 Aug 2022 13:22:58: 22000000 INFO @ Tue, 02 Aug 2022 13:23:00: 11000000 INFO @ Tue, 02 Aug 2022 13:23:00: 17000000 INFO @ Tue, 02 Aug 2022 13:23:03: 23000000 INFO @ Tue, 02 Aug 2022 13:23:05: 12000000 INFO @ Tue, 02 Aug 2022 13:23:06: 18000000 INFO @ Tue, 02 Aug 2022 13:23:09: 24000000 INFO @ Tue, 02 Aug 2022 13:23:10: 13000000 INFO @ Tue, 02 Aug 2022 13:23:11: 19000000 INFO @ Tue, 02 Aug 2022 13:23:14: 25000000 INFO @ Tue, 02 Aug 2022 13:23:16: 14000000 INFO @ Tue, 02 Aug 2022 13:23:16: 20000000 INFO @ Tue, 02 Aug 2022 13:23:19: 26000000 INFO @ Tue, 02 Aug 2022 13:23:21: 15000000 INFO @ Tue, 02 Aug 2022 13:23:21: 21000000 INFO @ Tue, 02 Aug 2022 13:23:25: 27000000 INFO @ Tue, 02 Aug 2022 13:23:26: 22000000 INFO @ Tue, 02 Aug 2022 13:23:26: 16000000 INFO @ Tue, 02 Aug 2022 13:23:26: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 13:23:26: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 13:23:26: #1 total tags in treatment: 12653310 INFO @ Tue, 02 Aug 2022 13:23:26: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:23:26: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:23:26: #1 tags after filtering in treatment: 12040031 INFO @ Tue, 02 Aug 2022 13:23:26: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 13:23:26: #1 finished! INFO @ Tue, 02 Aug 2022 13:23:26: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:23:26: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:23:27: #2 number of paired peaks: 83 WARNING @ Tue, 02 Aug 2022 13:23:27: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:23:27: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:23:31: 23000000 INFO @ Tue, 02 Aug 2022 13:23:31: 17000000 INFO @ Tue, 02 Aug 2022 13:23:36: 24000000 INFO @ Tue, 02 Aug 2022 13:23:36: 18000000 INFO @ Tue, 02 Aug 2022 13:23:41: 25000000 INFO @ Tue, 02 Aug 2022 13:23:41: 19000000 INFO @ Tue, 02 Aug 2022 13:23:46: 26000000 INFO @ Tue, 02 Aug 2022 13:23:46: 20000000 INFO @ Tue, 02 Aug 2022 13:23:51: 27000000 INFO @ Tue, 02 Aug 2022 13:23:51: 21000000 INFO @ Tue, 02 Aug 2022 13:23:52: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 13:23:52: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 13:23:52: #1 total tags in treatment: 12653310 INFO @ Tue, 02 Aug 2022 13:23:52: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:23:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:23:52: #1 tags after filtering in treatment: 12040031 INFO @ Tue, 02 Aug 2022 13:23:52: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 13:23:52: #1 finished! INFO @ Tue, 02 Aug 2022 13:23:52: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:23:52: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:23:53: #2 number of paired peaks: 83 WARNING @ Tue, 02 Aug 2022 13:23:53: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:23:53: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 02 Aug 2022 13:23:56: 22000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 02 Aug 2022 13:24:01: 23000000 INFO @ Tue, 02 Aug 2022 13:24:06: 24000000 INFO @ Tue, 02 Aug 2022 13:24:10: 25000000 INFO @ Tue, 02 Aug 2022 13:24:15: 26000000 INFO @ Tue, 02 Aug 2022 13:24:20: 27000000 INFO @ Tue, 02 Aug 2022 13:24:21: #1 tag size is determined as 50 bps INFO @ Tue, 02 Aug 2022 13:24:21: #1 tag size = 50 INFO @ Tue, 02 Aug 2022 13:24:21: #1 total tags in treatment: 12653310 INFO @ Tue, 02 Aug 2022 13:24:21: #1 user defined the maximum tags... INFO @ Tue, 02 Aug 2022 13:24:21: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 02 Aug 2022 13:24:21: #1 tags after filtering in treatment: 12040031 INFO @ Tue, 02 Aug 2022 13:24:21: #1 Redundant rate of treatment: 0.05 INFO @ Tue, 02 Aug 2022 13:24:21: #1 finished! INFO @ Tue, 02 Aug 2022 13:24:21: #2 Build Peak Model... INFO @ Tue, 02 Aug 2022 13:24:21: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 02 Aug 2022 13:24:22: #2 number of paired peaks: 83 WARNING @ Tue, 02 Aug 2022 13:24:22: Too few paired peaks (83) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 02 Aug 2022 13:24:22: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX5847884/ERX5847884.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。