Job ID = 14168201 SRX = ERX3978952 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 81857734 spots for ERR3976023/ERR3976023.sra Written 81857734 spots for ERR3976023/ERR3976023.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169857 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 04:21:03 81857734 reads; of these: 81857734 (100.00%) were paired; of these: 10817109 (13.21%) aligned concordantly 0 times 57171558 (69.84%) aligned concordantly exactly 1 time 13869067 (16.94%) aligned concordantly >1 times ---- 10817109 pairs aligned concordantly 0 times; of these: 2213398 (20.46%) aligned discordantly 1 time ---- 8603711 pairs aligned 0 times concordantly or discordantly; of these: 17207422 mates make up the pairs; of these: 8009525 (46.55%) aligned 0 times 4720845 (27.43%) aligned exactly 1 time 4477052 (26.02%) aligned >1 times 95.11% overall alignment rate Time searching: 04:21:03 Overall time: 04:21:03 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 64 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 54852936 / 72850328 = 0.7530 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:06:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:06:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:06:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:07:01: 1000000 INFO @ Fri, 10 Dec 2021 21:07:06: 2000000 INFO @ Fri, 10 Dec 2021 21:07:12: 3000000 INFO @ Fri, 10 Dec 2021 21:07:18: 4000000 INFO @ Fri, 10 Dec 2021 21:07:23: 5000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:07:25: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:07:25: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:07:25: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:07:29: 6000000 INFO @ Fri, 10 Dec 2021 21:07:32: 1000000 INFO @ Fri, 10 Dec 2021 21:07:36: 7000000 INFO @ Fri, 10 Dec 2021 21:07:38: 2000000 INFO @ Fri, 10 Dec 2021 21:07:42: 8000000 INFO @ Fri, 10 Dec 2021 21:07:44: 3000000 INFO @ Fri, 10 Dec 2021 21:07:48: 9000000 INFO @ Fri, 10 Dec 2021 21:07:50: 4000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 21:07:55: 10000000 INFO @ Fri, 10 Dec 2021 21:07:55: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 21:07:55: #1 read tag files... INFO @ Fri, 10 Dec 2021 21:07:55: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 21:07:57: 5000000 INFO @ Fri, 10 Dec 2021 21:08:02: 11000000 INFO @ Fri, 10 Dec 2021 21:08:03: 1000000 INFO @ Fri, 10 Dec 2021 21:08:04: 6000000 INFO @ Fri, 10 Dec 2021 21:08:09: 12000000 INFO @ Fri, 10 Dec 2021 21:08:11: 2000000 INFO @ Fri, 10 Dec 2021 21:08:12: 7000000 INFO @ Fri, 10 Dec 2021 21:08:16: 13000000 INFO @ Fri, 10 Dec 2021 21:08:19: 8000000 INFO @ Fri, 10 Dec 2021 21:08:19: 3000000 INFO @ Fri, 10 Dec 2021 21:08:23: 14000000 INFO @ Fri, 10 Dec 2021 21:08:26: 9000000 INFO @ Fri, 10 Dec 2021 21:08:27: 4000000 INFO @ Fri, 10 Dec 2021 21:08:30: 15000000 INFO @ Fri, 10 Dec 2021 21:08:33: 10000000 INFO @ Fri, 10 Dec 2021 21:08:35: 5000000 INFO @ Fri, 10 Dec 2021 21:08:37: 16000000 INFO @ Fri, 10 Dec 2021 21:08:40: 11000000 INFO @ Fri, 10 Dec 2021 21:08:43: 6000000 INFO @ Fri, 10 Dec 2021 21:08:45: 17000000 INFO @ Fri, 10 Dec 2021 21:08:47: 12000000 INFO @ Fri, 10 Dec 2021 21:08:50: 7000000 INFO @ Fri, 10 Dec 2021 21:08:52: 18000000 INFO @ Fri, 10 Dec 2021 21:08:54: 13000000 INFO @ Fri, 10 Dec 2021 21:08:58: 8000000 INFO @ Fri, 10 Dec 2021 21:08:59: 19000000 INFO @ Fri, 10 Dec 2021 21:09:01: 14000000 INFO @ Fri, 10 Dec 2021 21:09:06: 20000000 INFO @ Fri, 10 Dec 2021 21:09:06: 9000000 INFO @ Fri, 10 Dec 2021 21:09:08: 15000000 INFO @ Fri, 10 Dec 2021 21:09:13: 21000000 INFO @ Fri, 10 Dec 2021 21:09:14: 10000000 INFO @ Fri, 10 Dec 2021 21:09:15: 16000000 INFO @ Fri, 10 Dec 2021 21:09:20: 22000000 INFO @ Fri, 10 Dec 2021 21:09:22: 11000000 INFO @ Fri, 10 Dec 2021 21:09:22: 17000000 INFO @ Fri, 10 Dec 2021 21:09:27: 23000000 INFO @ Fri, 10 Dec 2021 21:09:29: 18000000 INFO @ Fri, 10 Dec 2021 21:09:29: 12000000 INFO @ Fri, 10 Dec 2021 21:09:34: 24000000 INFO @ Fri, 10 Dec 2021 21:09:36: 19000000 INFO @ Fri, 10 Dec 2021 21:09:37: 13000000 INFO @ Fri, 10 Dec 2021 21:09:41: 25000000 INFO @ Fri, 10 Dec 2021 21:09:43: 20000000 INFO @ Fri, 10 Dec 2021 21:09:45: 14000000 INFO @ Fri, 10 Dec 2021 21:09:48: 26000000 INFO @ Fri, 10 Dec 2021 21:09:50: 21000000 INFO @ Fri, 10 Dec 2021 21:09:53: 15000000 INFO @ Fri, 10 Dec 2021 21:09:55: 27000000 INFO @ Fri, 10 Dec 2021 21:09:57: 22000000 INFO @ Fri, 10 Dec 2021 21:10:00: 16000000 INFO @ Fri, 10 Dec 2021 21:10:02: 28000000 INFO @ Fri, 10 Dec 2021 21:10:04: 23000000 INFO @ Fri, 10 Dec 2021 21:10:08: 17000000 INFO @ Fri, 10 Dec 2021 21:10:09: 29000000 INFO @ Fri, 10 Dec 2021 21:10:11: 24000000 INFO @ Fri, 10 Dec 2021 21:10:16: 30000000 INFO @ Fri, 10 Dec 2021 21:10:16: 18000000 INFO @ Fri, 10 Dec 2021 21:10:18: 25000000 INFO @ Fri, 10 Dec 2021 21:10:23: 31000000 INFO @ Fri, 10 Dec 2021 21:10:24: 19000000 INFO @ Fri, 10 Dec 2021 21:10:25: 26000000 INFO @ Fri, 10 Dec 2021 21:10:30: 32000000 INFO @ Fri, 10 Dec 2021 21:10:31: 20000000 INFO @ Fri, 10 Dec 2021 21:10:32: 27000000 INFO @ Fri, 10 Dec 2021 21:10:37: 33000000 INFO @ Fri, 10 Dec 2021 21:10:39: 21000000 INFO @ Fri, 10 Dec 2021 21:10:39: 28000000 INFO @ Fri, 10 Dec 2021 21:10:44: 34000000 INFO @ Fri, 10 Dec 2021 21:10:46: 29000000 INFO @ Fri, 10 Dec 2021 21:10:46: 22000000 INFO @ Fri, 10 Dec 2021 21:10:51: 35000000 INFO @ Fri, 10 Dec 2021 21:10:53: 30000000 INFO @ Fri, 10 Dec 2021 21:10:54: 23000000 INFO @ Fri, 10 Dec 2021 21:10:58: 36000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 21:11:00: 31000000 INFO @ Fri, 10 Dec 2021 21:11:02: 24000000 INFO @ Fri, 10 Dec 2021 21:11:05: 37000000 INFO @ Fri, 10 Dec 2021 21:11:07: 32000000 INFO @ Fri, 10 Dec 2021 21:11:10: 25000000 INFO @ Fri, 10 Dec 2021 21:11:12: 38000000 INFO @ Fri, 10 Dec 2021 21:11:14: 33000000 INFO @ Fri, 10 Dec 2021 21:11:17: 26000000 INFO @ Fri, 10 Dec 2021 21:11:19: 39000000 INFO @ Fri, 10 Dec 2021 21:11:21: 34000000 INFO @ Fri, 10 Dec 2021 21:11:25: 27000000 INFO @ Fri, 10 Dec 2021 21:11:26: 40000000 INFO @ Fri, 10 Dec 2021 21:11:28: 35000000 INFO @ Fri, 10 Dec 2021 21:11:33: 28000000 INFO @ Fri, 10 Dec 2021 21:11:33: 41000000 INFO @ Fri, 10 Dec 2021 21:11:35: 36000000 INFO @ Fri, 10 Dec 2021 21:11:40: 42000000 INFO @ Fri, 10 Dec 2021 21:11:41: 29000000 INFO @ Fri, 10 Dec 2021 21:11:42: 37000000 INFO @ Fri, 10 Dec 2021 21:11:47: 43000000 INFO @ Fri, 10 Dec 2021 21:11:48: 30000000 INFO @ Fri, 10 Dec 2021 21:11:49: 38000000 INFO @ Fri, 10 Dec 2021 21:11:54: 44000000 INFO @ Fri, 10 Dec 2021 21:11:56: 31000000 INFO @ Fri, 10 Dec 2021 21:11:56: 39000000 INFO @ Fri, 10 Dec 2021 21:12:00: 45000000 INFO @ Fri, 10 Dec 2021 21:12:03: 40000000 INFO @ Fri, 10 Dec 2021 21:12:04: 32000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 21:12:07: 46000000 INFO @ Fri, 10 Dec 2021 21:12:07: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 21:12:07: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 21:12:07: #1 total tags in treatment: 17710504 INFO @ Fri, 10 Dec 2021 21:12:07: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:12:07: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:12:08: #1 tags after filtering in treatment: 15369023 INFO @ Fri, 10 Dec 2021 21:12:08: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 10 Dec 2021 21:12:08: #1 finished! INFO @ Fri, 10 Dec 2021 21:12:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:12:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:12:09: #2 number of paired peaks: 916 WARNING @ Fri, 10 Dec 2021 21:12:09: Fewer paired peaks (916) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 916 pairs to build model! INFO @ Fri, 10 Dec 2021 21:12:09: start model_add_line... INFO @ Fri, 10 Dec 2021 21:12:09: start X-correlation... INFO @ Fri, 10 Dec 2021 21:12:09: end of X-cor INFO @ Fri, 10 Dec 2021 21:12:09: #2 finished! INFO @ Fri, 10 Dec 2021 21:12:09: #2 predicted fragment length is 151 bps INFO @ Fri, 10 Dec 2021 21:12:09: #2 alternative fragment length(s) may be 151 bps INFO @ Fri, 10 Dec 2021 21:12:09: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.05_model.r WARNING @ Fri, 10 Dec 2021 21:12:09: #2 Since the d (151) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 21:12:09: #2 You may need to consider one of the other alternative d(s): 151 WARNING @ Fri, 10 Dec 2021 21:12:09: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 21:12:09: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:12:09: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:12:10: 41000000 INFO @ Fri, 10 Dec 2021 21:12:11: 33000000 INFO @ Fri, 10 Dec 2021 21:12:16: 42000000 INFO @ Fri, 10 Dec 2021 21:12:19: 34000000 INFO @ Fri, 10 Dec 2021 21:12:23: 43000000 INFO @ Fri, 10 Dec 2021 21:12:27: 35000000 INFO @ Fri, 10 Dec 2021 21:12:30: 44000000 INFO @ Fri, 10 Dec 2021 21:12:34: 36000000 INFO @ Fri, 10 Dec 2021 21:12:36: 45000000 INFO @ Fri, 10 Dec 2021 21:12:42: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:12:42: 37000000 INFO @ Fri, 10 Dec 2021 21:12:43: 46000000 INFO @ Fri, 10 Dec 2021 21:12:43: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 21:12:43: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 21:12:43: #1 total tags in treatment: 17710504 INFO @ Fri, 10 Dec 2021 21:12:43: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:12:43: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:12:43: #1 tags after filtering in treatment: 15369023 INFO @ Fri, 10 Dec 2021 21:12:43: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 10 Dec 2021 21:12:43: #1 finished! INFO @ Fri, 10 Dec 2021 21:12:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:12:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:12:44: #2 number of paired peaks: 916 WARNING @ Fri, 10 Dec 2021 21:12:44: Fewer paired peaks (916) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 916 pairs to build model! INFO @ Fri, 10 Dec 2021 21:12:44: start model_add_line... INFO @ Fri, 10 Dec 2021 21:12:45: start X-correlation... INFO @ Fri, 10 Dec 2021 21:12:45: end of X-cor INFO @ Fri, 10 Dec 2021 21:12:45: #2 finished! INFO @ Fri, 10 Dec 2021 21:12:45: #2 predicted fragment length is 151 bps INFO @ Fri, 10 Dec 2021 21:12:45: #2 alternative fragment length(s) may be 151 bps INFO @ Fri, 10 Dec 2021 21:12:45: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.10_model.r WARNING @ Fri, 10 Dec 2021 21:12:45: #2 Since the d (151) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 21:12:45: #2 You may need to consider one of the other alternative d(s): 151 WARNING @ Fri, 10 Dec 2021 21:12:45: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 21:12:45: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:12:45: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:12:50: 38000000 INFO @ Fri, 10 Dec 2021 21:12:57: 39000000 INFO @ Fri, 10 Dec 2021 21:12:58: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.05_peaks.xls INFO @ Fri, 10 Dec 2021 21:12:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:12:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.05_summits.bed INFO @ Fri, 10 Dec 2021 21:12:58: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (13698 records, 4 fields): 13 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:13:04: 40000000 INFO @ Fri, 10 Dec 2021 21:13:11: 41000000 INFO @ Fri, 10 Dec 2021 21:13:18: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:13:18: 42000000 INFO @ Fri, 10 Dec 2021 21:13:25: 43000000 INFO @ Fri, 10 Dec 2021 21:13:32: 44000000 INFO @ Fri, 10 Dec 2021 21:13:34: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.10_peaks.xls INFO @ Fri, 10 Dec 2021 21:13:34: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:13:34: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.10_summits.bed INFO @ Fri, 10 Dec 2021 21:13:34: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (8279 records, 4 fields): 9 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 21:13:39: 45000000 INFO @ Fri, 10 Dec 2021 21:13:45: 46000000 INFO @ Fri, 10 Dec 2021 21:13:46: #1 tag size is determined as 80 bps INFO @ Fri, 10 Dec 2021 21:13:46: #1 tag size = 80 INFO @ Fri, 10 Dec 2021 21:13:46: #1 total tags in treatment: 17710504 INFO @ Fri, 10 Dec 2021 21:13:46: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 21:13:46: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 21:13:46: #1 tags after filtering in treatment: 15369023 INFO @ Fri, 10 Dec 2021 21:13:46: #1 Redundant rate of treatment: 0.13 INFO @ Fri, 10 Dec 2021 21:13:46: #1 finished! INFO @ Fri, 10 Dec 2021 21:13:46: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 21:13:46: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 21:13:47: #2 number of paired peaks: 916 WARNING @ Fri, 10 Dec 2021 21:13:47: Fewer paired peaks (916) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 916 pairs to build model! INFO @ Fri, 10 Dec 2021 21:13:47: start model_add_line... INFO @ Fri, 10 Dec 2021 21:13:47: start X-correlation... INFO @ Fri, 10 Dec 2021 21:13:47: end of X-cor INFO @ Fri, 10 Dec 2021 21:13:47: #2 finished! INFO @ Fri, 10 Dec 2021 21:13:47: #2 predicted fragment length is 151 bps INFO @ Fri, 10 Dec 2021 21:13:47: #2 alternative fragment length(s) may be 151 bps INFO @ Fri, 10 Dec 2021 21:13:47: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.20_model.r WARNING @ Fri, 10 Dec 2021 21:13:47: #2 Since the d (151) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 21:13:47: #2 You may need to consider one of the other alternative d(s): 151 WARNING @ Fri, 10 Dec 2021 21:13:47: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 21:13:47: #3 Call peaks... INFO @ Fri, 10 Dec 2021 21:13:47: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 21:14:19: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 21:14:35: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.20_peaks.xls INFO @ Fri, 10 Dec 2021 21:14:35: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 21:14:35: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978952/ERX3978952.20_summits.bed INFO @ Fri, 10 Dec 2021 21:14:35: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3332 records, 4 fields): 5 millis CompletedMACS2peakCalling