Job ID = 14168029 SRX = ERX3978816 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28969497 spots for ERR3975861/ERR3975861.sra Written 28969497 spots for ERR3975861/ERR3975861.sra Read 29353959 spots for ERR3975884/ERR3975884.sra Written 29353959 spots for ERR3975884/ERR3975884.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169007 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:30:26 58323456 reads; of these: 58323456 (100.00%) were paired; of these: 28130859 (48.23%) aligned concordantly 0 times 5234464 (8.97%) aligned concordantly exactly 1 time 24958133 (42.79%) aligned concordantly >1 times ---- 28130859 pairs aligned concordantly 0 times; of these: 1749988 (6.22%) aligned discordantly 1 time ---- 26380871 pairs aligned 0 times concordantly or discordantly; of these: 52761742 mates make up the pairs; of these: 20025384 (37.95%) aligned 0 times 804966 (1.53%) aligned exactly 1 time 31931392 (60.52%) aligned >1 times 82.83% overall alignment rate Time searching: 01:30:27 Overall time: 01:30:27 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 68 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 22438155 / 31220075 = 0.7187 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:31:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:31:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:31:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:31:33: 1000000 INFO @ Fri, 10 Dec 2021 16:31:41: 2000000 INFO @ Fri, 10 Dec 2021 16:31:49: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:31:54: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:31:54: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:31:54: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:31:57: 4000000 INFO @ Fri, 10 Dec 2021 16:32:02: 1000000 INFO @ Fri, 10 Dec 2021 16:32:06: 5000000 INFO @ Fri, 10 Dec 2021 16:32:09: 2000000 INFO @ Fri, 10 Dec 2021 16:32:15: 6000000 INFO @ Fri, 10 Dec 2021 16:32:17: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:32:24: 4000000 INFO @ Fri, 10 Dec 2021 16:32:24: 7000000 INFO @ Fri, 10 Dec 2021 16:32:24: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:32:24: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:32:24: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:32:31: 5000000 INFO @ Fri, 10 Dec 2021 16:32:33: 8000000 INFO @ Fri, 10 Dec 2021 16:32:34: 1000000 INFO @ Fri, 10 Dec 2021 16:32:39: 6000000 INFO @ Fri, 10 Dec 2021 16:32:41: 9000000 INFO @ Fri, 10 Dec 2021 16:32:43: 2000000 INFO @ Fri, 10 Dec 2021 16:32:47: 7000000 INFO @ Fri, 10 Dec 2021 16:32:50: 10000000 INFO @ Fri, 10 Dec 2021 16:32:52: 3000000 INFO @ Fri, 10 Dec 2021 16:32:54: 8000000 INFO @ Fri, 10 Dec 2021 16:32:59: 11000000 INFO @ Fri, 10 Dec 2021 16:33:01: 4000000 INFO @ Fri, 10 Dec 2021 16:33:02: 9000000 INFO @ Fri, 10 Dec 2021 16:33:08: 12000000 INFO @ Fri, 10 Dec 2021 16:33:09: 10000000 INFO @ Fri, 10 Dec 2021 16:33:10: 5000000 INFO @ Fri, 10 Dec 2021 16:33:16: 13000000 INFO @ Fri, 10 Dec 2021 16:33:16: 11000000 INFO @ Fri, 10 Dec 2021 16:33:19: 6000000 INFO @ Fri, 10 Dec 2021 16:33:23: 12000000 INFO @ Fri, 10 Dec 2021 16:33:24: 14000000 INFO @ Fri, 10 Dec 2021 16:33:28: 7000000 INFO @ Fri, 10 Dec 2021 16:33:31: 13000000 INFO @ Fri, 10 Dec 2021 16:33:33: 15000000 INFO @ Fri, 10 Dec 2021 16:33:37: 8000000 INFO @ Fri, 10 Dec 2021 16:33:38: 14000000 INFO @ Fri, 10 Dec 2021 16:33:41: 16000000 INFO @ Fri, 10 Dec 2021 16:33:45: 15000000 INFO @ Fri, 10 Dec 2021 16:33:46: 9000000 INFO @ Fri, 10 Dec 2021 16:33:50: 17000000 INFO @ Fri, 10 Dec 2021 16:33:52: 16000000 INFO @ Fri, 10 Dec 2021 16:33:55: 10000000 INFO @ Fri, 10 Dec 2021 16:33:58: 18000000 INFO @ Fri, 10 Dec 2021 16:33:59: 17000000 INFO @ Fri, 10 Dec 2021 16:34:04: 11000000 INFO @ Fri, 10 Dec 2021 16:34:07: 18000000 INFO @ Fri, 10 Dec 2021 16:34:07: 19000000 INFO @ Fri, 10 Dec 2021 16:34:12: 12000000 INFO @ Fri, 10 Dec 2021 16:34:14: 19000000 INFO @ Fri, 10 Dec 2021 16:34:15: 20000000 INFO @ Fri, 10 Dec 2021 16:34:21: 13000000 INFO @ Fri, 10 Dec 2021 16:34:21: 20000000 INFO @ Fri, 10 Dec 2021 16:34:24: 21000000 INFO @ Fri, 10 Dec 2021 16:34:28: 21000000 INFO @ Fri, 10 Dec 2021 16:34:29: 14000000 INFO @ Fri, 10 Dec 2021 16:34:32: 22000000 INFO @ Fri, 10 Dec 2021 16:34:35: 22000000 INFO @ Fri, 10 Dec 2021 16:34:38: 15000000 INFO @ Fri, 10 Dec 2021 16:34:41: 23000000 INFO @ Fri, 10 Dec 2021 16:34:42: 23000000 INFO @ Fri, 10 Dec 2021 16:34:46: 16000000 INFO @ Fri, 10 Dec 2021 16:34:49: 24000000 INFO @ Fri, 10 Dec 2021 16:34:50: 24000000 INFO @ Fri, 10 Dec 2021 16:34:55: 17000000 INFO @ Fri, 10 Dec 2021 16:34:58: 25000000 INFO @ Fri, 10 Dec 2021 16:34:58: 25000000 INFO @ Fri, 10 Dec 2021 16:35:03: 18000000 INFO @ Fri, 10 Dec 2021 16:35:06: 26000000 INFO @ Fri, 10 Dec 2021 16:35:07: 26000000 INFO @ Fri, 10 Dec 2021 16:35:11: 19000000 INFO @ Fri, 10 Dec 2021 16:35:15: 27000000 INFO @ Fri, 10 Dec 2021 16:35:16: 27000000 INFO @ Fri, 10 Dec 2021 16:35:20: 20000000 INFO @ Fri, 10 Dec 2021 16:35:23: 28000000 INFO @ Fri, 10 Dec 2021 16:35:24: 28000000 INFO @ Fri, 10 Dec 2021 16:35:28: 21000000 INFO @ Fri, 10 Dec 2021 16:35:32: 29000000 INFO @ Fri, 10 Dec 2021 16:35:33: 29000000 INFO @ Fri, 10 Dec 2021 16:35:37: 22000000 INFO @ Fri, 10 Dec 2021 16:35:40: 30000000 INFO @ Fri, 10 Dec 2021 16:35:41: 30000000 INFO @ Fri, 10 Dec 2021 16:35:46: 23000000 INFO @ Fri, 10 Dec 2021 16:35:49: 31000000 INFO @ Fri, 10 Dec 2021 16:35:50: 31000000 INFO @ Fri, 10 Dec 2021 16:35:54: 24000000 INFO @ Fri, 10 Dec 2021 16:35:57: 32000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:35:58: 32000000 INFO @ Fri, 10 Dec 2021 16:36:03: 25000000 INFO @ Fri, 10 Dec 2021 16:36:05: 33000000 INFO @ Fri, 10 Dec 2021 16:36:06: 33000000 INFO @ Fri, 10 Dec 2021 16:36:11: 26000000 INFO @ Fri, 10 Dec 2021 16:36:13: 34000000 INFO @ Fri, 10 Dec 2021 16:36:13: 34000000 INFO @ Fri, 10 Dec 2021 16:36:20: 27000000 INFO @ Fri, 10 Dec 2021 16:36:21: 35000000 INFO @ Fri, 10 Dec 2021 16:36:21: 35000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:36:28: 28000000 INFO @ Fri, 10 Dec 2021 16:36:28: 36000000 INFO @ Fri, 10 Dec 2021 16:36:29: 36000000 INFO @ Fri, 10 Dec 2021 16:36:36: 37000000 INFO @ Fri, 10 Dec 2021 16:36:37: 29000000 INFO @ Fri, 10 Dec 2021 16:36:37: 37000000 INFO @ Fri, 10 Dec 2021 16:36:44: 38000000 INFO @ Fri, 10 Dec 2021 16:36:45: 38000000 INFO @ Fri, 10 Dec 2021 16:36:45: 30000000 INFO @ Fri, 10 Dec 2021 16:36:52: 39000000 INFO @ Fri, 10 Dec 2021 16:36:53: 39000000 INFO @ Fri, 10 Dec 2021 16:36:54: 31000000 INFO @ Fri, 10 Dec 2021 16:37:00: 40000000 INFO @ Fri, 10 Dec 2021 16:37:01: 40000000 INFO @ Fri, 10 Dec 2021 16:37:02: 32000000 INFO @ Fri, 10 Dec 2021 16:37:08: 41000000 INFO @ Fri, 10 Dec 2021 16:37:09: 41000000 INFO @ Fri, 10 Dec 2021 16:37:10: 33000000 INFO @ Fri, 10 Dec 2021 16:37:16: 42000000 INFO @ Fri, 10 Dec 2021 16:37:16: 42000000 INFO @ Fri, 10 Dec 2021 16:37:19: 34000000 INFO @ Fri, 10 Dec 2021 16:37:24: 43000000 INFO @ Fri, 10 Dec 2021 16:37:24: 43000000 INFO @ Fri, 10 Dec 2021 16:37:27: 35000000 INFO @ Fri, 10 Dec 2021 16:37:31: 44000000 INFO @ Fri, 10 Dec 2021 16:37:32: 44000000 INFO @ Fri, 10 Dec 2021 16:37:35: 36000000 INFO @ Fri, 10 Dec 2021 16:37:39: 45000000 INFO @ Fri, 10 Dec 2021 16:37:40: 45000000 INFO @ Fri, 10 Dec 2021 16:37:43: 37000000 INFO @ Fri, 10 Dec 2021 16:37:47: 46000000 INFO @ Fri, 10 Dec 2021 16:37:48: 46000000 INFO @ Fri, 10 Dec 2021 16:37:52: 38000000 INFO @ Fri, 10 Dec 2021 16:37:55: 47000000 INFO @ Fri, 10 Dec 2021 16:37:56: 47000000 INFO @ Fri, 10 Dec 2021 16:38:00: 39000000 INFO @ Fri, 10 Dec 2021 16:38:03: 48000000 INFO @ Fri, 10 Dec 2021 16:38:03: 48000000 INFO @ Fri, 10 Dec 2021 16:38:08: 40000000 INFO @ Fri, 10 Dec 2021 16:38:11: 49000000 INFO @ Fri, 10 Dec 2021 16:38:12: 49000000 INFO @ Fri, 10 Dec 2021 16:38:16: 41000000 INFO @ Fri, 10 Dec 2021 16:38:19: 50000000 INFO @ Fri, 10 Dec 2021 16:38:20: 50000000 INFO @ Fri, 10 Dec 2021 16:38:24: 42000000 INFO @ Fri, 10 Dec 2021 16:38:28: 51000000 INFO @ Fri, 10 Dec 2021 16:38:28: 51000000 INFO @ Fri, 10 Dec 2021 16:38:33: 43000000 INFO @ Fri, 10 Dec 2021 16:38:34: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:38:34: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:38:34: #1 total tags in treatment: 8218868 INFO @ Fri, 10 Dec 2021 16:38:34: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:38:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:38:34: #1 tags after filtering in treatment: 5306899 INFO @ Fri, 10 Dec 2021 16:38:34: #1 Redundant rate of treatment: 0.35 INFO @ Fri, 10 Dec 2021 16:38:34: #1 finished! INFO @ Fri, 10 Dec 2021 16:38:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:38:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:38:34: #2 number of paired peaks: 1548 INFO @ Fri, 10 Dec 2021 16:38:34: start model_add_line... INFO @ Fri, 10 Dec 2021 16:38:34: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:38:34: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:38:34: #1 total tags in treatment: 8218868 INFO @ Fri, 10 Dec 2021 16:38:34: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:38:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:38:34: start X-correlation... INFO @ Fri, 10 Dec 2021 16:38:34: end of X-cor INFO @ Fri, 10 Dec 2021 16:38:34: #2 finished! INFO @ Fri, 10 Dec 2021 16:38:34: #2 predicted fragment length is 149 bps INFO @ Fri, 10 Dec 2021 16:38:34: #2 alternative fragment length(s) may be 149 bps INFO @ Fri, 10 Dec 2021 16:38:34: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.05_model.r WARNING @ Fri, 10 Dec 2021 16:38:34: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:38:34: #2 You may need to consider one of the other alternative d(s): 149 WARNING @ Fri, 10 Dec 2021 16:38:34: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:38:34: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:38:34: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:38:35: #1 tags after filtering in treatment: 5306899 INFO @ Fri, 10 Dec 2021 16:38:35: #1 Redundant rate of treatment: 0.35 INFO @ Fri, 10 Dec 2021 16:38:35: #1 finished! INFO @ Fri, 10 Dec 2021 16:38:35: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:38:35: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:38:35: #2 number of paired peaks: 1548 INFO @ Fri, 10 Dec 2021 16:38:35: start model_add_line... INFO @ Fri, 10 Dec 2021 16:38:35: start X-correlation... INFO @ Fri, 10 Dec 2021 16:38:35: end of X-cor INFO @ Fri, 10 Dec 2021 16:38:35: #2 finished! INFO @ Fri, 10 Dec 2021 16:38:35: #2 predicted fragment length is 149 bps INFO @ Fri, 10 Dec 2021 16:38:35: #2 alternative fragment length(s) may be 149 bps INFO @ Fri, 10 Dec 2021 16:38:35: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.10_model.r WARNING @ Fri, 10 Dec 2021 16:38:35: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:38:35: #2 You may need to consider one of the other alternative d(s): 149 WARNING @ Fri, 10 Dec 2021 16:38:35: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:38:35: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:38:35: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:38:41: 44000000 INFO @ Fri, 10 Dec 2021 16:38:46: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:38:46: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:38:48: 45000000 INFO @ Fri, 10 Dec 2021 16:38:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.05_peaks.xls INFO @ Fri, 10 Dec 2021 16:38:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:38:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.05_summits.bed INFO @ Fri, 10 Dec 2021 16:38:52: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7086 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:38:52: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.10_peaks.xls INFO @ Fri, 10 Dec 2021 16:38:52: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:38:52: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.10_summits.bed INFO @ Fri, 10 Dec 2021 16:38:52: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3549 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:38:56: 46000000 INFO @ Fri, 10 Dec 2021 16:39:03: 47000000 INFO @ Fri, 10 Dec 2021 16:39:11: 48000000 INFO @ Fri, 10 Dec 2021 16:39:19: 49000000 INFO @ Fri, 10 Dec 2021 16:39:27: 50000000 INFO @ Fri, 10 Dec 2021 16:39:35: 51000000 INFO @ Fri, 10 Dec 2021 16:39:41: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:39:41: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:39:41: #1 total tags in treatment: 8218868 INFO @ Fri, 10 Dec 2021 16:39:41: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:39:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:39:41: #1 tags after filtering in treatment: 5306899 INFO @ Fri, 10 Dec 2021 16:39:41: #1 Redundant rate of treatment: 0.35 INFO @ Fri, 10 Dec 2021 16:39:41: #1 finished! INFO @ Fri, 10 Dec 2021 16:39:41: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:39:41: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:39:42: #2 number of paired peaks: 1548 INFO @ Fri, 10 Dec 2021 16:39:42: start model_add_line... INFO @ Fri, 10 Dec 2021 16:39:42: start X-correlation... INFO @ Fri, 10 Dec 2021 16:39:42: end of X-cor INFO @ Fri, 10 Dec 2021 16:39:42: #2 finished! INFO @ Fri, 10 Dec 2021 16:39:42: #2 predicted fragment length is 149 bps INFO @ Fri, 10 Dec 2021 16:39:42: #2 alternative fragment length(s) may be 149 bps INFO @ Fri, 10 Dec 2021 16:39:42: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.20_model.r WARNING @ Fri, 10 Dec 2021 16:39:42: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:39:42: #2 You may need to consider one of the other alternative d(s): 149 WARNING @ Fri, 10 Dec 2021 16:39:42: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:39:42: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:39:42: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:39:53: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:39:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.20_peaks.xls INFO @ Fri, 10 Dec 2021 16:39:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:39:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978816/ERX3978816.20_summits.bed INFO @ Fri, 10 Dec 2021 16:39:59: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1494 records, 4 fields): 3 millis CompletedMACS2peakCalling