Job ID = 14168025 SRX = ERX3978813 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 28162740 spots for ERR3975856/ERR3975856.sra Written 28162740 spots for ERR3975856/ERR3975856.sra Read 27832200 spots for ERR3975859/ERR3975859.sra Written 27832200 spots for ERR3975859/ERR3975859.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168999 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:30:38 55994940 reads; of these: 55994940 (100.00%) were paired; of these: 16751607 (29.92%) aligned concordantly 0 times 7704670 (13.76%) aligned concordantly exactly 1 time 31538663 (56.32%) aligned concordantly >1 times ---- 16751607 pairs aligned concordantly 0 times; of these: 1474587 (8.80%) aligned discordantly 1 time ---- 15277020 pairs aligned 0 times concordantly or discordantly; of these: 30554040 mates make up the pairs; of these: 10959798 (35.87%) aligned 0 times 506168 (1.66%) aligned exactly 1 time 19088074 (62.47%) aligned >1 times 90.21% overall alignment rate Time searching: 01:30:40 Overall time: 01:30:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 68 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 27778835 / 40062076 = 0.6934 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:28:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:28:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:28:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:28:52: 1000000 INFO @ Fri, 10 Dec 2021 16:29:02: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:29:12: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:29:12: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:29:12: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:29:12: 3000000 INFO @ Fri, 10 Dec 2021 16:29:23: 4000000 INFO @ Fri, 10 Dec 2021 16:29:23: 1000000 INFO @ Fri, 10 Dec 2021 16:29:34: 5000000 INFO @ Fri, 10 Dec 2021 16:29:34: 2000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:29:42: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:29:42: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:29:42: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:29:45: 6000000 INFO @ Fri, 10 Dec 2021 16:29:45: 3000000 INFO @ Fri, 10 Dec 2021 16:29:54: 1000000 INFO @ Fri, 10 Dec 2021 16:29:56: 7000000 INFO @ Fri, 10 Dec 2021 16:29:56: 4000000 INFO @ Fri, 10 Dec 2021 16:30:05: 2000000 INFO @ Fri, 10 Dec 2021 16:30:07: 5000000 INFO @ Fri, 10 Dec 2021 16:30:07: 8000000 INFO @ Fri, 10 Dec 2021 16:30:16: 3000000 INFO @ Fri, 10 Dec 2021 16:30:18: 6000000 INFO @ Fri, 10 Dec 2021 16:30:18: 9000000 INFO @ Fri, 10 Dec 2021 16:30:27: 4000000 INFO @ Fri, 10 Dec 2021 16:30:29: 7000000 INFO @ Fri, 10 Dec 2021 16:30:30: 10000000 INFO @ Fri, 10 Dec 2021 16:30:38: 5000000 INFO @ Fri, 10 Dec 2021 16:30:40: 8000000 INFO @ Fri, 10 Dec 2021 16:30:41: 11000000 INFO @ Fri, 10 Dec 2021 16:30:49: 6000000 INFO @ Fri, 10 Dec 2021 16:30:51: 9000000 INFO @ Fri, 10 Dec 2021 16:30:52: 12000000 INFO @ Fri, 10 Dec 2021 16:31:00: 7000000 INFO @ Fri, 10 Dec 2021 16:31:02: 10000000 INFO @ Fri, 10 Dec 2021 16:31:03: 13000000 INFO @ Fri, 10 Dec 2021 16:31:11: 8000000 INFO @ Fri, 10 Dec 2021 16:31:13: 11000000 INFO @ Fri, 10 Dec 2021 16:31:15: 14000000 INFO @ Fri, 10 Dec 2021 16:31:22: 9000000 INFO @ Fri, 10 Dec 2021 16:31:24: 12000000 INFO @ Fri, 10 Dec 2021 16:31:26: 15000000 INFO @ Fri, 10 Dec 2021 16:31:33: 10000000 INFO @ Fri, 10 Dec 2021 16:31:35: 13000000 INFO @ Fri, 10 Dec 2021 16:31:37: 16000000 INFO @ Fri, 10 Dec 2021 16:31:44: 11000000 INFO @ Fri, 10 Dec 2021 16:31:46: 14000000 INFO @ Fri, 10 Dec 2021 16:31:48: 17000000 INFO @ Fri, 10 Dec 2021 16:31:55: 12000000 INFO @ Fri, 10 Dec 2021 16:31:57: 15000000 INFO @ Fri, 10 Dec 2021 16:31:58: 18000000 INFO @ Fri, 10 Dec 2021 16:32:06: 13000000 INFO @ Fri, 10 Dec 2021 16:32:08: 16000000 INFO @ Fri, 10 Dec 2021 16:32:09: 19000000 INFO @ Fri, 10 Dec 2021 16:32:18: 14000000 INFO @ Fri, 10 Dec 2021 16:32:19: 17000000 INFO @ Fri, 10 Dec 2021 16:32:20: 20000000 INFO @ Fri, 10 Dec 2021 16:32:29: 15000000 INFO @ Fri, 10 Dec 2021 16:32:30: 18000000 INFO @ Fri, 10 Dec 2021 16:32:30: 21000000 INFO @ Fri, 10 Dec 2021 16:32:40: 16000000 INFO @ Fri, 10 Dec 2021 16:32:40: 19000000 INFO @ Fri, 10 Dec 2021 16:32:41: 22000000 INFO @ Fri, 10 Dec 2021 16:32:51: 20000000 INFO @ Fri, 10 Dec 2021 16:32:51: 17000000 INFO @ Fri, 10 Dec 2021 16:32:52: 23000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:33:01: 21000000 INFO @ Fri, 10 Dec 2021 16:33:02: 18000000 INFO @ Fri, 10 Dec 2021 16:33:03: 24000000 INFO @ Fri, 10 Dec 2021 16:33:12: 22000000 INFO @ Fri, 10 Dec 2021 16:33:13: 19000000 INFO @ Fri, 10 Dec 2021 16:33:14: 25000000 INFO @ Fri, 10 Dec 2021 16:33:23: 23000000 INFO @ Fri, 10 Dec 2021 16:33:24: 20000000 INFO @ Fri, 10 Dec 2021 16:33:25: 26000000 INFO @ Fri, 10 Dec 2021 16:33:33: 24000000 INFO @ Fri, 10 Dec 2021 16:33:34: 21000000 INFO @ Fri, 10 Dec 2021 16:33:36: 27000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:33:44: 25000000 INFO @ Fri, 10 Dec 2021 16:33:45: 22000000 INFO @ Fri, 10 Dec 2021 16:33:47: 28000000 INFO @ Fri, 10 Dec 2021 16:33:55: 26000000 INFO @ Fri, 10 Dec 2021 16:33:56: 23000000 INFO @ Fri, 10 Dec 2021 16:33:59: 29000000 INFO @ Fri, 10 Dec 2021 16:34:05: 27000000 INFO @ Fri, 10 Dec 2021 16:34:07: 24000000 INFO @ Fri, 10 Dec 2021 16:34:10: 30000000 INFO @ Fri, 10 Dec 2021 16:34:16: 28000000 INFO @ Fri, 10 Dec 2021 16:34:18: 25000000 INFO @ Fri, 10 Dec 2021 16:34:21: 31000000 INFO @ Fri, 10 Dec 2021 16:34:27: 29000000 INFO @ Fri, 10 Dec 2021 16:34:28: 26000000 INFO @ Fri, 10 Dec 2021 16:34:32: 32000000 INFO @ Fri, 10 Dec 2021 16:34:37: 30000000 INFO @ Fri, 10 Dec 2021 16:34:39: 27000000 INFO @ Fri, 10 Dec 2021 16:34:43: 33000000 INFO @ Fri, 10 Dec 2021 16:34:48: 31000000 INFO @ Fri, 10 Dec 2021 16:34:50: 28000000 INFO @ Fri, 10 Dec 2021 16:34:54: 34000000 INFO @ Fri, 10 Dec 2021 16:34:59: 32000000 INFO @ Fri, 10 Dec 2021 16:35:01: 29000000 INFO @ Fri, 10 Dec 2021 16:35:05: 35000000 INFO @ Fri, 10 Dec 2021 16:35:10: 33000000 INFO @ Fri, 10 Dec 2021 16:35:12: 30000000 INFO @ Fri, 10 Dec 2021 16:35:16: 36000000 INFO @ Fri, 10 Dec 2021 16:35:20: 34000000 INFO @ Fri, 10 Dec 2021 16:35:23: 31000000 INFO @ Fri, 10 Dec 2021 16:35:27: 37000000 INFO @ Fri, 10 Dec 2021 16:35:31: 35000000 INFO @ Fri, 10 Dec 2021 16:35:34: 32000000 INFO @ Fri, 10 Dec 2021 16:35:38: 38000000 INFO @ Fri, 10 Dec 2021 16:35:42: 36000000 INFO @ Fri, 10 Dec 2021 16:35:44: 33000000 INFO @ Fri, 10 Dec 2021 16:35:49: 39000000 INFO @ Fri, 10 Dec 2021 16:35:52: 37000000 INFO @ Fri, 10 Dec 2021 16:35:55: 34000000 INFO @ Fri, 10 Dec 2021 16:36:00: 40000000 INFO @ Fri, 10 Dec 2021 16:36:02: 38000000 INFO @ Fri, 10 Dec 2021 16:36:06: 35000000 INFO @ Fri, 10 Dec 2021 16:36:11: 41000000 INFO @ Fri, 10 Dec 2021 16:36:13: 39000000 INFO @ Fri, 10 Dec 2021 16:36:17: 36000000 INFO @ Fri, 10 Dec 2021 16:36:22: 42000000 INFO @ Fri, 10 Dec 2021 16:36:23: 40000000 INFO @ Fri, 10 Dec 2021 16:36:28: 37000000 INFO @ Fri, 10 Dec 2021 16:36:34: 43000000 INFO @ Fri, 10 Dec 2021 16:36:34: 41000000 INFO @ Fri, 10 Dec 2021 16:36:39: 38000000 INFO @ Fri, 10 Dec 2021 16:36:45: 42000000 INFO @ Fri, 10 Dec 2021 16:36:45: 44000000 INFO @ Fri, 10 Dec 2021 16:36:50: 39000000 INFO @ Fri, 10 Dec 2021 16:36:56: 43000000 INFO @ Fri, 10 Dec 2021 16:36:57: 45000000 INFO @ Fri, 10 Dec 2021 16:37:01: 40000000 INFO @ Fri, 10 Dec 2021 16:37:02: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:37:02: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:37:02: #1 total tags in treatment: 11713734 INFO @ Fri, 10 Dec 2021 16:37:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:37:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:37:02: #1 tags after filtering in treatment: 8321887 INFO @ Fri, 10 Dec 2021 16:37:02: #1 Redundant rate of treatment: 0.29 INFO @ Fri, 10 Dec 2021 16:37:02: #1 finished! INFO @ Fri, 10 Dec 2021 16:37:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:37:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:37:03: #2 number of paired peaks: 717 WARNING @ Fri, 10 Dec 2021 16:37:03: Fewer paired peaks (717) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 717 pairs to build model! INFO @ Fri, 10 Dec 2021 16:37:03: start model_add_line... INFO @ Fri, 10 Dec 2021 16:37:03: start X-correlation... INFO @ Fri, 10 Dec 2021 16:37:03: end of X-cor INFO @ Fri, 10 Dec 2021 16:37:03: #2 finished! INFO @ Fri, 10 Dec 2021 16:37:03: #2 predicted fragment length is 148 bps INFO @ Fri, 10 Dec 2021 16:37:03: #2 alternative fragment length(s) may be 148 bps INFO @ Fri, 10 Dec 2021 16:37:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.05_model.r WARNING @ Fri, 10 Dec 2021 16:37:03: #2 Since the d (148) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:37:03: #2 You may need to consider one of the other alternative d(s): 148 WARNING @ Fri, 10 Dec 2021 16:37:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:37:03: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:37:03: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:37:07: 44000000 INFO @ Fri, 10 Dec 2021 16:37:12: 41000000 INFO @ Fri, 10 Dec 2021 16:37:18: 45000000 INFO @ Fri, 10 Dec 2021 16:37:20: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:37:22: 42000000 INFO @ Fri, 10 Dec 2021 16:37:23: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:37:23: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:37:23: #1 total tags in treatment: 11713734 INFO @ Fri, 10 Dec 2021 16:37:23: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:37:23: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:37:24: #1 tags after filtering in treatment: 8321887 INFO @ Fri, 10 Dec 2021 16:37:24: #1 Redundant rate of treatment: 0.29 INFO @ Fri, 10 Dec 2021 16:37:24: #1 finished! INFO @ Fri, 10 Dec 2021 16:37:24: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:37:24: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:37:24: #2 number of paired peaks: 717 WARNING @ Fri, 10 Dec 2021 16:37:24: Fewer paired peaks (717) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 717 pairs to build model! INFO @ Fri, 10 Dec 2021 16:37:24: start model_add_line... INFO @ Fri, 10 Dec 2021 16:37:24: start X-correlation... INFO @ Fri, 10 Dec 2021 16:37:24: end of X-cor INFO @ Fri, 10 Dec 2021 16:37:24: #2 finished! INFO @ Fri, 10 Dec 2021 16:37:24: #2 predicted fragment length is 148 bps INFO @ Fri, 10 Dec 2021 16:37:24: #2 alternative fragment length(s) may be 148 bps INFO @ Fri, 10 Dec 2021 16:37:24: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.10_model.r WARNING @ Fri, 10 Dec 2021 16:37:24: #2 Since the d (148) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:37:24: #2 You may need to consider one of the other alternative d(s): 148 WARNING @ Fri, 10 Dec 2021 16:37:24: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:37:24: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:37:24: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:37:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.05_peaks.xls INFO @ Fri, 10 Dec 2021 16:37:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:37:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.05_summits.bed INFO @ Fri, 10 Dec 2021 16:37:29: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (7179 records, 4 fields): 8 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:37:32: 43000000 INFO @ Fri, 10 Dec 2021 16:37:41: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:37:42: 44000000 INFO @ Fri, 10 Dec 2021 16:37:50: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.10_peaks.xls INFO @ Fri, 10 Dec 2021 16:37:50: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:37:50: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.10_summits.bed INFO @ Fri, 10 Dec 2021 16:37:50: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (3444 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:37:52: 45000000 INFO @ Fri, 10 Dec 2021 16:37:56: #1 tag size is determined as 121 bps INFO @ Fri, 10 Dec 2021 16:37:56: #1 tag size = 121 INFO @ Fri, 10 Dec 2021 16:37:56: #1 total tags in treatment: 11713734 INFO @ Fri, 10 Dec 2021 16:37:56: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:37:56: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:37:57: #1 tags after filtering in treatment: 8321887 INFO @ Fri, 10 Dec 2021 16:37:57: #1 Redundant rate of treatment: 0.29 INFO @ Fri, 10 Dec 2021 16:37:57: #1 finished! INFO @ Fri, 10 Dec 2021 16:37:57: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:37:57: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:37:57: #2 number of paired peaks: 717 WARNING @ Fri, 10 Dec 2021 16:37:57: Fewer paired peaks (717) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 717 pairs to build model! INFO @ Fri, 10 Dec 2021 16:37:57: start model_add_line... INFO @ Fri, 10 Dec 2021 16:37:57: start X-correlation... INFO @ Fri, 10 Dec 2021 16:37:57: end of X-cor INFO @ Fri, 10 Dec 2021 16:37:57: #2 finished! INFO @ Fri, 10 Dec 2021 16:37:57: #2 predicted fragment length is 148 bps INFO @ Fri, 10 Dec 2021 16:37:57: #2 alternative fragment length(s) may be 148 bps INFO @ Fri, 10 Dec 2021 16:37:57: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.20_model.r WARNING @ Fri, 10 Dec 2021 16:37:57: #2 Since the d (148) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:37:57: #2 You may need to consider one of the other alternative d(s): 148 WARNING @ Fri, 10 Dec 2021 16:37:57: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:37:57: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:37:57: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:38:15: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:38:23: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.20_peaks.xls INFO @ Fri, 10 Dec 2021 16:38:23: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:38:23: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978813/ERX3978813.20_summits.bed INFO @ Fri, 10 Dec 2021 16:38:23: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1395 records, 4 fields): 2 millis CompletedMACS2peakCalling