Job ID = 14168021 SRX = ERX3978812 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 18758406 spots for ERR3975854/ERR3975854.sra Written 18758406 spots for ERR3975854/ERR3975854.sra Read 21585608 spots for ERR3975880/ERR3975880.sra Written 21585608 spots for ERR3975880/ERR3975880.sra fastq に変換しました。 bowtie でマッピング中... Your job 14169016 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:40:34 40344014 reads; of these: 40344014 (100.00%) were paired; of these: 6116603 (15.16%) aligned concordantly 0 times 25509353 (63.23%) aligned concordantly exactly 1 time 8718058 (21.61%) aligned concordantly >1 times ---- 6116603 pairs aligned concordantly 0 times; of these: 2437795 (39.86%) aligned discordantly 1 time ---- 3678808 pairs aligned 0 times concordantly or discordantly; of these: 7357616 mates make up the pairs; of these: 4670962 (63.48%) aligned 0 times 1047478 (14.24%) aligned exactly 1 time 1639176 (22.28%) aligned >1 times 94.21% overall alignment rate Time searching: 01:40:34 Overall time: 01:40:34 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 40 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 10168954 / 36460993 = 0.2789 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:32:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:32:18: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:32:18: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:32:26: 1000000 INFO @ Fri, 10 Dec 2021 16:32:34: 2000000 INFO @ Fri, 10 Dec 2021 16:32:42: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:32:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:32:48: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:32:48: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:32:50: 4000000 INFO @ Fri, 10 Dec 2021 16:32:56: 1000000 INFO @ Fri, 10 Dec 2021 16:32:58: 5000000 INFO @ Fri, 10 Dec 2021 16:33:03: 2000000 INFO @ Fri, 10 Dec 2021 16:33:07: 6000000 INFO @ Fri, 10 Dec 2021 16:33:10: 3000000 INFO @ Fri, 10 Dec 2021 16:33:15: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:33:18: 4000000 INFO @ Fri, 10 Dec 2021 16:33:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:33:18: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:33:18: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:33:24: 8000000 INFO @ Fri, 10 Dec 2021 16:33:25: 5000000 INFO @ Fri, 10 Dec 2021 16:33:26: 1000000 INFO @ Fri, 10 Dec 2021 16:33:32: 9000000 INFO @ Fri, 10 Dec 2021 16:33:33: 6000000 INFO @ Fri, 10 Dec 2021 16:33:34: 2000000 INFO @ Fri, 10 Dec 2021 16:33:40: 10000000 INFO @ Fri, 10 Dec 2021 16:33:40: 7000000 INFO @ Fri, 10 Dec 2021 16:33:41: 3000000 INFO @ Fri, 10 Dec 2021 16:33:48: 8000000 INFO @ Fri, 10 Dec 2021 16:33:49: 11000000 INFO @ Fri, 10 Dec 2021 16:33:49: 4000000 INFO @ Fri, 10 Dec 2021 16:33:56: 9000000 INFO @ Fri, 10 Dec 2021 16:33:56: 5000000 INFO @ Fri, 10 Dec 2021 16:33:57: 12000000 INFO @ Fri, 10 Dec 2021 16:34:04: 10000000 INFO @ Fri, 10 Dec 2021 16:34:04: 6000000 INFO @ Fri, 10 Dec 2021 16:34:05: 13000000 INFO @ Fri, 10 Dec 2021 16:34:11: 11000000 INFO @ Fri, 10 Dec 2021 16:34:11: 7000000 INFO @ Fri, 10 Dec 2021 16:34:14: 14000000 INFO @ Fri, 10 Dec 2021 16:34:19: 12000000 INFO @ Fri, 10 Dec 2021 16:34:19: 8000000 INFO @ Fri, 10 Dec 2021 16:34:22: 15000000 INFO @ Fri, 10 Dec 2021 16:34:26: 13000000 INFO @ Fri, 10 Dec 2021 16:34:26: 9000000 INFO @ Fri, 10 Dec 2021 16:34:31: 16000000 INFO @ Fri, 10 Dec 2021 16:34:34: 14000000 INFO @ Fri, 10 Dec 2021 16:34:34: 10000000 INFO @ Fri, 10 Dec 2021 16:34:39: 17000000 INFO @ Fri, 10 Dec 2021 16:34:41: 15000000 INFO @ Fri, 10 Dec 2021 16:34:41: 11000000 INFO @ Fri, 10 Dec 2021 16:34:47: 18000000 INFO @ Fri, 10 Dec 2021 16:34:49: 16000000 INFO @ Fri, 10 Dec 2021 16:34:49: 12000000 INFO @ Fri, 10 Dec 2021 16:34:56: 19000000 INFO @ Fri, 10 Dec 2021 16:34:56: 17000000 INFO @ Fri, 10 Dec 2021 16:34:57: 13000000 INFO @ Fri, 10 Dec 2021 16:35:04: 18000000 INFO @ Fri, 10 Dec 2021 16:35:04: 14000000 INFO @ Fri, 10 Dec 2021 16:35:04: 20000000 INFO @ Fri, 10 Dec 2021 16:35:12: 19000000 INFO @ Fri, 10 Dec 2021 16:35:12: 15000000 INFO @ Fri, 10 Dec 2021 16:35:13: 21000000 INFO @ Fri, 10 Dec 2021 16:35:19: 20000000 INFO @ Fri, 10 Dec 2021 16:35:19: 16000000 INFO @ Fri, 10 Dec 2021 16:35:21: 22000000 INFO @ Fri, 10 Dec 2021 16:35:27: 21000000 INFO @ Fri, 10 Dec 2021 16:35:27: 17000000 INFO @ Fri, 10 Dec 2021 16:35:30: 23000000 INFO @ Fri, 10 Dec 2021 16:35:34: 22000000 INFO @ Fri, 10 Dec 2021 16:35:34: 18000000 INFO @ Fri, 10 Dec 2021 16:35:38: 24000000 INFO @ Fri, 10 Dec 2021 16:35:42: 23000000 INFO @ Fri, 10 Dec 2021 16:35:42: 19000000 INFO @ Fri, 10 Dec 2021 16:35:46: 25000000 INFO @ Fri, 10 Dec 2021 16:35:49: 24000000 INFO @ Fri, 10 Dec 2021 16:35:50: 20000000 INFO @ Fri, 10 Dec 2021 16:35:55: 26000000 INFO @ Fri, 10 Dec 2021 16:35:57: 25000000 INFO @ Fri, 10 Dec 2021 16:35:57: 21000000 INFO @ Fri, 10 Dec 2021 16:36:03: 27000000 INFO @ Fri, 10 Dec 2021 16:36:05: 26000000 INFO @ Fri, 10 Dec 2021 16:36:05: 22000000 INFO @ Fri, 10 Dec 2021 16:36:11: 28000000 INFO @ Fri, 10 Dec 2021 16:36:12: 27000000 INFO @ Fri, 10 Dec 2021 16:36:12: 23000000 INFO @ Fri, 10 Dec 2021 16:36:20: 28000000 INFO @ Fri, 10 Dec 2021 16:36:20: 29000000 INFO @ Fri, 10 Dec 2021 16:36:20: 24000000 INFO @ Fri, 10 Dec 2021 16:36:27: 29000000 INFO @ Fri, 10 Dec 2021 16:36:27: 25000000 INFO @ Fri, 10 Dec 2021 16:36:28: 30000000 INFO @ Fri, 10 Dec 2021 16:36:35: 30000000 INFO @ Fri, 10 Dec 2021 16:36:35: 26000000 INFO @ Fri, 10 Dec 2021 16:36:37: 31000000 INFO @ Fri, 10 Dec 2021 16:36:42: 31000000 INFO @ Fri, 10 Dec 2021 16:36:43: 27000000 INFO @ Fri, 10 Dec 2021 16:36:45: 32000000 INFO @ Fri, 10 Dec 2021 16:36:50: 32000000 INFO @ Fri, 10 Dec 2021 16:36:50: 28000000 INFO @ Fri, 10 Dec 2021 16:36:53: 33000000 INFO @ Fri, 10 Dec 2021 16:36:57: 33000000 INFO @ Fri, 10 Dec 2021 16:36:58: 29000000 INFO @ Fri, 10 Dec 2021 16:37:01: 34000000 INFO @ Fri, 10 Dec 2021 16:37:05: 34000000 INFO @ Fri, 10 Dec 2021 16:37:05: 30000000 INFO @ Fri, 10 Dec 2021 16:37:10: 35000000 INFO @ Fri, 10 Dec 2021 16:37:12: 35000000 INFO @ Fri, 10 Dec 2021 16:37:13: 31000000 INFO @ Fri, 10 Dec 2021 16:37:18: 36000000 INFO @ Fri, 10 Dec 2021 16:37:20: 36000000 INFO @ Fri, 10 Dec 2021 16:37:20: 32000000 INFO @ Fri, 10 Dec 2021 16:37:27: 37000000 INFO @ Fri, 10 Dec 2021 16:37:28: 33000000 INFO @ Fri, 10 Dec 2021 16:37:28: 37000000 INFO @ Fri, 10 Dec 2021 16:37:35: 38000000 INFO @ Fri, 10 Dec 2021 16:37:35: 34000000 INFO @ Fri, 10 Dec 2021 16:37:36: 38000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:37:42: 35000000 INFO @ Fri, 10 Dec 2021 16:37:43: 39000000 INFO @ Fri, 10 Dec 2021 16:37:44: 39000000 INFO @ Fri, 10 Dec 2021 16:37:50: 36000000 INFO @ Fri, 10 Dec 2021 16:37:51: 40000000 INFO @ Fri, 10 Dec 2021 16:37:52: 40000000 INFO @ Fri, 10 Dec 2021 16:37:57: 37000000 INFO @ Fri, 10 Dec 2021 16:38:00: 41000000 INFO @ Fri, 10 Dec 2021 16:38:00: 41000000 INFO @ Fri, 10 Dec 2021 16:38:05: 38000000 INFO @ Fri, 10 Dec 2021 16:38:08: 42000000 INFO @ Fri, 10 Dec 2021 16:38:09: 42000000 INFO @ Fri, 10 Dec 2021 16:38:12: 39000000 INFO @ Fri, 10 Dec 2021 16:38:16: 43000000 INFO @ Fri, 10 Dec 2021 16:38:17: 43000000 INFO @ Fri, 10 Dec 2021 16:38:19: 40000000 INFO @ Fri, 10 Dec 2021 16:38:25: 44000000 INFO @ Fri, 10 Dec 2021 16:38:25: 44000000 INFO @ Fri, 10 Dec 2021 16:38:26: 41000000 INFO @ Fri, 10 Dec 2021 16:38:33: 45000000 INFO @ Fri, 10 Dec 2021 16:38:33: 42000000 INFO @ Fri, 10 Dec 2021 16:38:34: 45000000 INFO @ Fri, 10 Dec 2021 16:38:41: 43000000 INFO @ Fri, 10 Dec 2021 16:38:41: 46000000 INFO @ Fri, 10 Dec 2021 16:38:42: 46000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:38:48: 44000000 INFO @ Fri, 10 Dec 2021 16:38:50: 47000000 INFO @ Fri, 10 Dec 2021 16:38:50: 47000000 INFO @ Fri, 10 Dec 2021 16:38:56: 45000000 INFO @ Fri, 10 Dec 2021 16:38:58: 48000000 INFO @ Fri, 10 Dec 2021 16:38:59: 48000000 INFO @ Fri, 10 Dec 2021 16:39:03: 46000000 INFO @ Fri, 10 Dec 2021 16:39:06: 49000000 INFO @ Fri, 10 Dec 2021 16:39:07: 49000000 INFO @ Fri, 10 Dec 2021 16:39:10: 47000000 INFO @ Fri, 10 Dec 2021 16:39:14: 50000000 INFO @ Fri, 10 Dec 2021 16:39:15: 50000000 INFO @ Fri, 10 Dec 2021 16:39:17: 48000000 INFO @ Fri, 10 Dec 2021 16:39:23: 51000000 INFO @ Fri, 10 Dec 2021 16:39:23: 51000000 INFO @ Fri, 10 Dec 2021 16:39:25: 49000000 INFO @ Fri, 10 Dec 2021 16:39:31: 52000000 INFO @ Fri, 10 Dec 2021 16:39:31: 52000000 INFO @ Fri, 10 Dec 2021 16:39:32: 50000000 INFO @ Fri, 10 Dec 2021 16:39:39: 53000000 INFO @ Fri, 10 Dec 2021 16:39:39: 51000000 INFO @ Fri, 10 Dec 2021 16:39:40: 53000000 INFO @ Fri, 10 Dec 2021 16:39:46: 52000000 INFO @ Fri, 10 Dec 2021 16:39:47: 54000000 INFO @ Fri, 10 Dec 2021 16:39:48: 54000000 INFO @ Fri, 10 Dec 2021 16:39:54: 53000000 INFO @ Fri, 10 Dec 2021 16:39:55: 55000000 INFO @ Fri, 10 Dec 2021 16:39:56: 55000000 INFO @ Fri, 10 Dec 2021 16:40:01: 54000000 INFO @ Fri, 10 Dec 2021 16:40:01: #1 tag size is determined as 119 bps INFO @ Fri, 10 Dec 2021 16:40:01: #1 tag size = 119 INFO @ Fri, 10 Dec 2021 16:40:01: #1 total tags in treatment: 24465558 INFO @ Fri, 10 Dec 2021 16:40:01: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:40:01: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:40:02: #1 tags after filtering in treatment: 18023100 INFO @ Fri, 10 Dec 2021 16:40:02: #1 Redundant rate of treatment: 0.26 INFO @ Fri, 10 Dec 2021 16:40:02: #1 finished! INFO @ Fri, 10 Dec 2021 16:40:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:40:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:40:02: #1 tag size is determined as 119 bps INFO @ Fri, 10 Dec 2021 16:40:02: #1 tag size = 119 INFO @ Fri, 10 Dec 2021 16:40:02: #1 total tags in treatment: 24465558 INFO @ Fri, 10 Dec 2021 16:40:02: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:40:02: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:40:02: #1 tags after filtering in treatment: 18023100 INFO @ Fri, 10 Dec 2021 16:40:02: #1 Redundant rate of treatment: 0.26 INFO @ Fri, 10 Dec 2021 16:40:02: #1 finished! INFO @ Fri, 10 Dec 2021 16:40:02: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:40:02: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:40:03: #2 number of paired peaks: 2070 INFO @ Fri, 10 Dec 2021 16:40:03: start model_add_line... INFO @ Fri, 10 Dec 2021 16:40:03: start X-correlation... INFO @ Fri, 10 Dec 2021 16:40:03: end of X-cor INFO @ Fri, 10 Dec 2021 16:40:03: #2 finished! INFO @ Fri, 10 Dec 2021 16:40:03: #2 predicted fragment length is 149 bps INFO @ Fri, 10 Dec 2021 16:40:03: #2 alternative fragment length(s) may be 149 bps INFO @ Fri, 10 Dec 2021 16:40:03: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.05_model.r WARNING @ Fri, 10 Dec 2021 16:40:03: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:40:03: #2 You may need to consider one of the other alternative d(s): 149 WARNING @ Fri, 10 Dec 2021 16:40:03: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:40:03: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:40:03: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:40:04: #2 number of paired peaks: 2070 INFO @ Fri, 10 Dec 2021 16:40:04: start model_add_line... INFO @ Fri, 10 Dec 2021 16:40:04: start X-correlation... INFO @ Fri, 10 Dec 2021 16:40:04: end of X-cor INFO @ Fri, 10 Dec 2021 16:40:04: #2 finished! INFO @ Fri, 10 Dec 2021 16:40:04: #2 predicted fragment length is 149 bps INFO @ Fri, 10 Dec 2021 16:40:04: #2 alternative fragment length(s) may be 149 bps INFO @ Fri, 10 Dec 2021 16:40:04: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.10_model.r WARNING @ Fri, 10 Dec 2021 16:40:04: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:40:04: #2 You may need to consider one of the other alternative d(s): 149 WARNING @ Fri, 10 Dec 2021 16:40:04: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:40:04: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:40:04: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:40:08: 55000000 INFO @ Fri, 10 Dec 2021 16:40:13: #1 tag size is determined as 119 bps INFO @ Fri, 10 Dec 2021 16:40:13: #1 tag size = 119 INFO @ Fri, 10 Dec 2021 16:40:13: #1 total tags in treatment: 24465558 INFO @ Fri, 10 Dec 2021 16:40:13: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:40:13: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:40:13: #1 tags after filtering in treatment: 18023100 INFO @ Fri, 10 Dec 2021 16:40:13: #1 Redundant rate of treatment: 0.26 INFO @ Fri, 10 Dec 2021 16:40:13: #1 finished! INFO @ Fri, 10 Dec 2021 16:40:13: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:40:13: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:40:14: #2 number of paired peaks: 2070 INFO @ Fri, 10 Dec 2021 16:40:14: start model_add_line... INFO @ Fri, 10 Dec 2021 16:40:14: start X-correlation... INFO @ Fri, 10 Dec 2021 16:40:14: end of X-cor INFO @ Fri, 10 Dec 2021 16:40:14: #2 finished! INFO @ Fri, 10 Dec 2021 16:40:14: #2 predicted fragment length is 149 bps INFO @ Fri, 10 Dec 2021 16:40:14: #2 alternative fragment length(s) may be 149 bps INFO @ Fri, 10 Dec 2021 16:40:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.20_model.r WARNING @ Fri, 10 Dec 2021 16:40:14: #2 Since the d (149) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:40:14: #2 You may need to consider one of the other alternative d(s): 149 WARNING @ Fri, 10 Dec 2021 16:40:14: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:40:14: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:40:14: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:40:40: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:40:40: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:40:51: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:40:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.05_peaks.xls INFO @ Fri, 10 Dec 2021 16:40:57: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:40:57: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.05_summits.bed INFO @ Fri, 10 Dec 2021 16:40:57: Done! INFO @ Fri, 10 Dec 2021 16:40:57: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.10_peaks.xls INFO @ Fri, 10 Dec 2021 16:40:58: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:40:58: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.10_summits.bed INFO @ Fri, 10 Dec 2021 16:40:58: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (12523 records, 4 fields): 32 millis CompletedMACS2peakCalling pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (9187 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:41:08: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.20_peaks.xls INFO @ Fri, 10 Dec 2021 16:41:08: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:41:08: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978812/ERX3978812.20_summits.bed INFO @ Fri, 10 Dec 2021 16:41:08: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (5505 records, 4 fields): 16 millis CompletedMACS2peakCalling