Job ID = 14167772 SRX = ERX3978806 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 25569486 spots for ERR3975845/ERR3975845.sra Written 25569486 spots for ERR3975845/ERR3975845.sra Read 20411953 spots for ERR3975886/ERR3975886.sra Written 20411953 spots for ERR3975886/ERR3975886.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168956 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 02:15:17 45981439 reads; of these: 45981439 (100.00%) were paired; of these: 10251010 (22.29%) aligned concordantly 0 times 25220644 (54.85%) aligned concordantly exactly 1 time 10509785 (22.86%) aligned concordantly >1 times ---- 10251010 pairs aligned concordantly 0 times; of these: 4125372 (40.24%) aligned discordantly 1 time ---- 6125638 pairs aligned 0 times concordantly or discordantly; of these: 12251276 mates make up the pairs; of these: 7751220 (63.27%) aligned 0 times 779884 (6.37%) aligned exactly 1 time 3720172 (30.37%) aligned >1 times 91.57% overall alignment rate Time searching: 02:15:18 Overall time: 02:15:18 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 52 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 13737959 / 39425182 = 0.3485 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:11:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:11:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:11:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:11:12: 1000000 INFO @ Fri, 10 Dec 2021 16:11:21: 2000000 INFO @ Fri, 10 Dec 2021 16:11:29: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:11:34: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:11:34: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:11:34: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:11:39: 4000000 INFO @ Fri, 10 Dec 2021 16:11:42: 1000000 INFO @ Fri, 10 Dec 2021 16:11:48: 5000000 INFO @ Fri, 10 Dec 2021 16:11:49: 2000000 INFO @ Fri, 10 Dec 2021 16:11:56: 3000000 INFO @ Fri, 10 Dec 2021 16:11:57: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 16:12:03: 4000000 INFO @ Fri, 10 Dec 2021 16:12:04: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 16:12:04: #1 read tag files... INFO @ Fri, 10 Dec 2021 16:12:04: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 16:12:07: 7000000 INFO @ Fri, 10 Dec 2021 16:12:10: 5000000 INFO @ Fri, 10 Dec 2021 16:12:12: 1000000 INFO @ Fri, 10 Dec 2021 16:12:16: 8000000 INFO @ Fri, 10 Dec 2021 16:12:17: 6000000 INFO @ Fri, 10 Dec 2021 16:12:19: 2000000 INFO @ Fri, 10 Dec 2021 16:12:24: 7000000 INFO @ Fri, 10 Dec 2021 16:12:25: 9000000 INFO @ Fri, 10 Dec 2021 16:12:26: 3000000 INFO @ Fri, 10 Dec 2021 16:12:31: 8000000 INFO @ Fri, 10 Dec 2021 16:12:33: 4000000 INFO @ Fri, 10 Dec 2021 16:12:34: 10000000 INFO @ Fri, 10 Dec 2021 16:12:38: 9000000 INFO @ Fri, 10 Dec 2021 16:12:40: 5000000 INFO @ Fri, 10 Dec 2021 16:12:43: 11000000 INFO @ Fri, 10 Dec 2021 16:12:45: 10000000 INFO @ Fri, 10 Dec 2021 16:12:47: 6000000 INFO @ Fri, 10 Dec 2021 16:12:52: 11000000 INFO @ Fri, 10 Dec 2021 16:12:53: 12000000 INFO @ Fri, 10 Dec 2021 16:12:54: 7000000 INFO @ Fri, 10 Dec 2021 16:12:59: 12000000 INFO @ Fri, 10 Dec 2021 16:13:01: 8000000 INFO @ Fri, 10 Dec 2021 16:13:02: 13000000 INFO @ Fri, 10 Dec 2021 16:13:06: 13000000 INFO @ Fri, 10 Dec 2021 16:13:08: 9000000 INFO @ Fri, 10 Dec 2021 16:13:11: 14000000 INFO @ Fri, 10 Dec 2021 16:13:13: 14000000 INFO @ Fri, 10 Dec 2021 16:13:15: 10000000 INFO @ Fri, 10 Dec 2021 16:13:18: 15000000 INFO @ Fri, 10 Dec 2021 16:13:20: 15000000 INFO @ Fri, 10 Dec 2021 16:13:21: 11000000 INFO @ Fri, 10 Dec 2021 16:13:25: 16000000 INFO @ Fri, 10 Dec 2021 16:13:27: 16000000 INFO @ Fri, 10 Dec 2021 16:13:28: 12000000 INFO @ Fri, 10 Dec 2021 16:13:31: 17000000 INFO @ Fri, 10 Dec 2021 16:13:34: 17000000 INFO @ Fri, 10 Dec 2021 16:13:35: 13000000 INFO @ Fri, 10 Dec 2021 16:13:38: 18000000 INFO @ Fri, 10 Dec 2021 16:13:40: 18000000 INFO @ Fri, 10 Dec 2021 16:13:42: 14000000 INFO @ Fri, 10 Dec 2021 16:13:45: 19000000 INFO @ Fri, 10 Dec 2021 16:13:47: 19000000 INFO @ Fri, 10 Dec 2021 16:13:49: 15000000 INFO @ Fri, 10 Dec 2021 16:13:52: 20000000 INFO @ Fri, 10 Dec 2021 16:13:54: 20000000 INFO @ Fri, 10 Dec 2021 16:13:56: 16000000 INFO @ Fri, 10 Dec 2021 16:13:59: 21000000 INFO @ Fri, 10 Dec 2021 16:14:01: 21000000 INFO @ Fri, 10 Dec 2021 16:14:02: 17000000 INFO @ Fri, 10 Dec 2021 16:14:05: 22000000 INFO @ Fri, 10 Dec 2021 16:14:08: 22000000 INFO @ Fri, 10 Dec 2021 16:14:09: 18000000 INFO @ Fri, 10 Dec 2021 16:14:12: 23000000 INFO @ Fri, 10 Dec 2021 16:14:15: 23000000 INFO @ Fri, 10 Dec 2021 16:14:16: 19000000 INFO @ Fri, 10 Dec 2021 16:14:19: 24000000 INFO @ Fri, 10 Dec 2021 16:14:21: 24000000 INFO @ Fri, 10 Dec 2021 16:14:23: 20000000 INFO @ Fri, 10 Dec 2021 16:14:26: 25000000 INFO @ Fri, 10 Dec 2021 16:14:28: 25000000 INFO @ Fri, 10 Dec 2021 16:14:30: 21000000 INFO @ Fri, 10 Dec 2021 16:14:33: 26000000 INFO @ Fri, 10 Dec 2021 16:14:35: 26000000 INFO @ Fri, 10 Dec 2021 16:14:37: 22000000 INFO @ Fri, 10 Dec 2021 16:14:40: 27000000 INFO @ Fri, 10 Dec 2021 16:14:42: 27000000 INFO @ Fri, 10 Dec 2021 16:14:44: 23000000 INFO @ Fri, 10 Dec 2021 16:14:47: 28000000 INFO @ Fri, 10 Dec 2021 16:14:49: 28000000 INFO @ Fri, 10 Dec 2021 16:14:50: 24000000 INFO @ Fri, 10 Dec 2021 16:14:53: 29000000 INFO @ Fri, 10 Dec 2021 16:14:56: 29000000 INFO @ Fri, 10 Dec 2021 16:14:57: 25000000 INFO @ Fri, 10 Dec 2021 16:15:01: 30000000 INFO @ Fri, 10 Dec 2021 16:15:03: 30000000 INFO @ Fri, 10 Dec 2021 16:15:04: 26000000 INFO @ Fri, 10 Dec 2021 16:15:08: 31000000 INFO @ Fri, 10 Dec 2021 16:15:10: 31000000 INFO @ Fri, 10 Dec 2021 16:15:11: 27000000 INFO @ Fri, 10 Dec 2021 16:15:15: 32000000 INFO @ Fri, 10 Dec 2021 16:15:17: 32000000 INFO @ Fri, 10 Dec 2021 16:15:18: 28000000 INFO @ Fri, 10 Dec 2021 16:15:23: 33000000 INFO @ Fri, 10 Dec 2021 16:15:24: 33000000 INFO @ Fri, 10 Dec 2021 16:15:25: 29000000 INFO @ Fri, 10 Dec 2021 16:15:30: 34000000 INFO @ Fri, 10 Dec 2021 16:15:31: 34000000 INFO @ Fri, 10 Dec 2021 16:15:32: 30000000 INFO @ Fri, 10 Dec 2021 16:15:37: 35000000 INFO @ Fri, 10 Dec 2021 16:15:38: 35000000 INFO @ Fri, 10 Dec 2021 16:15:39: 31000000 INFO @ Fri, 10 Dec 2021 16:15:44: 36000000 INFO @ Fri, 10 Dec 2021 16:15:45: 36000000 INFO @ Fri, 10 Dec 2021 16:15:46: 32000000 INFO @ Fri, 10 Dec 2021 16:15:51: 37000000 INFO @ Fri, 10 Dec 2021 16:15:52: 37000000 INFO @ Fri, 10 Dec 2021 16:15:54: 33000000 INFO @ Fri, 10 Dec 2021 16:15:58: 38000000 INFO @ Fri, 10 Dec 2021 16:15:59: 38000000 INFO @ Fri, 10 Dec 2021 16:16:00: 34000000 INFO @ Fri, 10 Dec 2021 16:16:05: 39000000 INFO @ Fri, 10 Dec 2021 16:16:06: 39000000 INFO @ Fri, 10 Dec 2021 16:16:07: 35000000 INFO @ Fri, 10 Dec 2021 16:16:12: 40000000 INFO @ Fri, 10 Dec 2021 16:16:13: 40000000 INFO @ Fri, 10 Dec 2021 16:16:14: 36000000 INFO @ Fri, 10 Dec 2021 16:16:18: 41000000 INFO @ Fri, 10 Dec 2021 16:16:20: 41000000 INFO @ Fri, 10 Dec 2021 16:16:21: 37000000 INFO @ Fri, 10 Dec 2021 16:16:25: 42000000 INFO @ Fri, 10 Dec 2021 16:16:26: 42000000 INFO @ Fri, 10 Dec 2021 16:16:28: 38000000 INFO @ Fri, 10 Dec 2021 16:16:32: 43000000 INFO @ Fri, 10 Dec 2021 16:16:33: 43000000 INFO @ Fri, 10 Dec 2021 16:16:35: 39000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 16:16:39: 44000000 INFO @ Fri, 10 Dec 2021 16:16:40: 44000000 INFO @ Fri, 10 Dec 2021 16:16:41: 40000000 INFO @ Fri, 10 Dec 2021 16:16:46: 45000000 INFO @ Fri, 10 Dec 2021 16:16:47: 45000000 INFO @ Fri, 10 Dec 2021 16:16:48: 41000000 INFO @ Fri, 10 Dec 2021 16:16:53: 46000000 INFO @ Fri, 10 Dec 2021 16:16:54: 46000000 INFO @ Fri, 10 Dec 2021 16:16:55: 42000000 INFO @ Fri, 10 Dec 2021 16:16:59: 47000000 INFO @ Fri, 10 Dec 2021 16:17:01: 47000000 INFO @ Fri, 10 Dec 2021 16:17:02: 43000000 INFO @ Fri, 10 Dec 2021 16:17:06: 48000000 INFO @ Fri, 10 Dec 2021 16:17:08: 48000000 INFO @ Fri, 10 Dec 2021 16:17:08: 44000000 INFO @ Fri, 10 Dec 2021 16:17:13: 49000000 INFO @ Fri, 10 Dec 2021 16:17:15: 49000000 INFO @ Fri, 10 Dec 2021 16:17:15: 45000000 INFO @ Fri, 10 Dec 2021 16:17:20: 50000000 INFO @ Fri, 10 Dec 2021 16:17:22: 50000000 INFO @ Fri, 10 Dec 2021 16:17:22: 46000000 INFO @ Fri, 10 Dec 2021 16:17:27: 51000000 INFO @ Fri, 10 Dec 2021 16:17:28: 51000000 INFO @ Fri, 10 Dec 2021 16:17:29: 47000000 INFO @ Fri, 10 Dec 2021 16:17:34: 52000000 INFO @ Fri, 10 Dec 2021 16:17:35: 52000000 INFO @ Fri, 10 Dec 2021 16:17:36: 48000000 INFO @ Fri, 10 Dec 2021 16:17:41: 53000000 INFO @ Fri, 10 Dec 2021 16:17:42: 53000000 INFO @ Fri, 10 Dec 2021 16:17:43: 49000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 16:17:47: 54000000 INFO @ Fri, 10 Dec 2021 16:17:49: 54000000 INFO @ Fri, 10 Dec 2021 16:17:50: 50000000 INFO @ Fri, 10 Dec 2021 16:17:54: 55000000 INFO @ Fri, 10 Dec 2021 16:17:56: 55000000 INFO @ Fri, 10 Dec 2021 16:17:57: 51000000 INFO @ Fri, 10 Dec 2021 16:18:01: 56000000 INFO @ Fri, 10 Dec 2021 16:18:03: 56000000 INFO @ Fri, 10 Dec 2021 16:18:03: 52000000 INFO @ Fri, 10 Dec 2021 16:18:06: #1 tag size is determined as 120 bps INFO @ Fri, 10 Dec 2021 16:18:06: #1 tag size = 120 INFO @ Fri, 10 Dec 2021 16:18:06: #1 total tags in treatment: 22840936 INFO @ Fri, 10 Dec 2021 16:18:06: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:18:06: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:18:07: #1 tags after filtering in treatment: 15912759 INFO @ Fri, 10 Dec 2021 16:18:07: #1 Redundant rate of treatment: 0.30 INFO @ Fri, 10 Dec 2021 16:18:07: #1 finished! INFO @ Fri, 10 Dec 2021 16:18:07: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:18:07: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:18:08: #1 tag size is determined as 120 bps INFO @ Fri, 10 Dec 2021 16:18:08: #1 tag size = 120 INFO @ Fri, 10 Dec 2021 16:18:08: #1 total tags in treatment: 22840936 INFO @ Fri, 10 Dec 2021 16:18:08: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:18:08: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:18:08: #2 number of paired peaks: 2826 INFO @ Fri, 10 Dec 2021 16:18:08: start model_add_line... INFO @ Fri, 10 Dec 2021 16:18:08: start X-correlation... INFO @ Fri, 10 Dec 2021 16:18:08: end of X-cor INFO @ Fri, 10 Dec 2021 16:18:08: #2 finished! INFO @ Fri, 10 Dec 2021 16:18:08: #2 predicted fragment length is 127 bps INFO @ Fri, 10 Dec 2021 16:18:08: #2 alternative fragment length(s) may be 127 bps INFO @ Fri, 10 Dec 2021 16:18:08: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.05_model.r WARNING @ Fri, 10 Dec 2021 16:18:08: #2 Since the d (127) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:18:08: #2 You may need to consider one of the other alternative d(s): 127 WARNING @ Fri, 10 Dec 2021 16:18:08: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:18:08: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:18:08: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:18:08: #1 tags after filtering in treatment: 15912759 INFO @ Fri, 10 Dec 2021 16:18:08: #1 Redundant rate of treatment: 0.30 INFO @ Fri, 10 Dec 2021 16:18:08: #1 finished! INFO @ Fri, 10 Dec 2021 16:18:08: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:18:08: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:18:10: #2 number of paired peaks: 2826 INFO @ Fri, 10 Dec 2021 16:18:10: start model_add_line... INFO @ Fri, 10 Dec 2021 16:18:10: start X-correlation... INFO @ Fri, 10 Dec 2021 16:18:10: end of X-cor INFO @ Fri, 10 Dec 2021 16:18:10: #2 finished! INFO @ Fri, 10 Dec 2021 16:18:10: #2 predicted fragment length is 127 bps INFO @ Fri, 10 Dec 2021 16:18:10: #2 alternative fragment length(s) may be 127 bps INFO @ Fri, 10 Dec 2021 16:18:10: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.10_model.r WARNING @ Fri, 10 Dec 2021 16:18:10: #2 Since the d (127) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:18:10: #2 You may need to consider one of the other alternative d(s): 127 WARNING @ Fri, 10 Dec 2021 16:18:10: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:18:10: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:18:10: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:18:10: 53000000 INFO @ Fri, 10 Dec 2021 16:18:16: 54000000 INFO @ Fri, 10 Dec 2021 16:18:23: 55000000 INFO @ Fri, 10 Dec 2021 16:18:29: 56000000 INFO @ Fri, 10 Dec 2021 16:18:34: #1 tag size is determined as 120 bps INFO @ Fri, 10 Dec 2021 16:18:34: #1 tag size = 120 INFO @ Fri, 10 Dec 2021 16:18:34: #1 total tags in treatment: 22840936 INFO @ Fri, 10 Dec 2021 16:18:34: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 16:18:34: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 16:18:34: #1 tags after filtering in treatment: 15912759 INFO @ Fri, 10 Dec 2021 16:18:34: #1 Redundant rate of treatment: 0.30 INFO @ Fri, 10 Dec 2021 16:18:34: #1 finished! INFO @ Fri, 10 Dec 2021 16:18:34: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 16:18:34: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 16:18:36: #2 number of paired peaks: 2826 INFO @ Fri, 10 Dec 2021 16:18:36: start model_add_line... INFO @ Fri, 10 Dec 2021 16:18:36: start X-correlation... INFO @ Fri, 10 Dec 2021 16:18:36: end of X-cor INFO @ Fri, 10 Dec 2021 16:18:36: #2 finished! INFO @ Fri, 10 Dec 2021 16:18:36: #2 predicted fragment length is 127 bps INFO @ Fri, 10 Dec 2021 16:18:36: #2 alternative fragment length(s) may be 127 bps INFO @ Fri, 10 Dec 2021 16:18:36: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.20_model.r WARNING @ Fri, 10 Dec 2021 16:18:36: #2 Since the d (127) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 16:18:36: #2 You may need to consider one of the other alternative d(s): 127 WARNING @ Fri, 10 Dec 2021 16:18:36: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 16:18:36: #3 Call peaks... INFO @ Fri, 10 Dec 2021 16:18:36: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 16:18:43: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:18:43: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:18:59: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.10_peaks.xls INFO @ Fri, 10 Dec 2021 16:18:59: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:18:59: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.10_summits.bed INFO @ Fri, 10 Dec 2021 16:18:59: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (11345 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:19:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.05_peaks.xls INFO @ Fri, 10 Dec 2021 16:19:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:19:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.05_summits.bed INFO @ Fri, 10 Dec 2021 16:19:00: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (14972 records, 4 fields): 22 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 16:19:10: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 16:19:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.20_peaks.xls INFO @ Fri, 10 Dec 2021 16:19:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 16:19:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978806/ERX3978806.20_summits.bed INFO @ Fri, 10 Dec 2021 16:19:26: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (7198 records, 4 fields): 8 millis CompletedMACS2peakCalling