Job ID = 14167768 SRX = ERX3978805 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 27026486 spots for ERR3975844/ERR3975844.sra Written 27026486 spots for ERR3975844/ERR3975844.sra Read 24775912 spots for ERR3975868/ERR3975868.sra Written 24775912 spots for ERR3975868/ERR3975868.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168850 ("srTdm6") has been submitted Time loading reference: 00:00:01 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:47:39 51802398 reads; of these: 51802398 (100.00%) were paired; of these: 14333229 (27.67%) aligned concordantly 0 times 27259058 (52.62%) aligned concordantly exactly 1 time 10210111 (19.71%) aligned concordantly >1 times ---- 14333229 pairs aligned concordantly 0 times; of these: 5502362 (38.39%) aligned discordantly 1 time ---- 8830867 pairs aligned 0 times concordantly or discordantly; of these: 17661734 mates make up the pairs; of these: 12880797 (72.93%) aligned 0 times 959533 (5.43%) aligned exactly 1 time 3821404 (21.64%) aligned >1 times 87.57% overall alignment rate Time searching: 01:47:40 Overall time: 01:47:40 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 60 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 14468768 / 42498845 = 0.3405 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:45:27: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:45:27: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:45:27: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:45:36: 1000000 INFO @ Fri, 10 Dec 2021 15:45:46: 2000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:45:55: 3000000 INFO @ Fri, 10 Dec 2021 15:45:57: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:45:57: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:45:57: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:46:05: 4000000 INFO @ Fri, 10 Dec 2021 15:46:06: 1000000 INFO @ Fri, 10 Dec 2021 15:46:15: 2000000 INFO @ Fri, 10 Dec 2021 15:46:15: 5000000 BedGraph に変換中... INFO @ Fri, 10 Dec 2021 15:46:24: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:46:26: 6000000 INFO @ Fri, 10 Dec 2021 15:46:26: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:46:26: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:46:26: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:46:33: 4000000 INFO @ Fri, 10 Dec 2021 15:46:36: 7000000 INFO @ Fri, 10 Dec 2021 15:46:37: 1000000 INFO @ Fri, 10 Dec 2021 15:46:43: 5000000 INFO @ Fri, 10 Dec 2021 15:46:47: 8000000 INFO @ Fri, 10 Dec 2021 15:46:48: 2000000 INFO @ Fri, 10 Dec 2021 15:46:52: 6000000 INFO @ Fri, 10 Dec 2021 15:46:58: 9000000 INFO @ Fri, 10 Dec 2021 15:46:58: 3000000 INFO @ Fri, 10 Dec 2021 15:47:01: 7000000 INFO @ Fri, 10 Dec 2021 15:47:08: 10000000 INFO @ Fri, 10 Dec 2021 15:47:09: 4000000 INFO @ Fri, 10 Dec 2021 15:47:11: 8000000 INFO @ Fri, 10 Dec 2021 15:47:19: 11000000 INFO @ Fri, 10 Dec 2021 15:47:20: 5000000 INFO @ Fri, 10 Dec 2021 15:47:20: 9000000 INFO @ Fri, 10 Dec 2021 15:47:29: 10000000 INFO @ Fri, 10 Dec 2021 15:47:29: 12000000 INFO @ Fri, 10 Dec 2021 15:47:31: 6000000 INFO @ Fri, 10 Dec 2021 15:47:38: 11000000 INFO @ Fri, 10 Dec 2021 15:47:39: 13000000 INFO @ Fri, 10 Dec 2021 15:47:41: 7000000 INFO @ Fri, 10 Dec 2021 15:47:47: 12000000 INFO @ Fri, 10 Dec 2021 15:47:50: 14000000 INFO @ Fri, 10 Dec 2021 15:47:52: 8000000 INFO @ Fri, 10 Dec 2021 15:47:56: 13000000 INFO @ Fri, 10 Dec 2021 15:48:01: 15000000 INFO @ Fri, 10 Dec 2021 15:48:03: 9000000 INFO @ Fri, 10 Dec 2021 15:48:06: 14000000 INFO @ Fri, 10 Dec 2021 15:48:11: 16000000 INFO @ Fri, 10 Dec 2021 15:48:13: 10000000 INFO @ Fri, 10 Dec 2021 15:48:15: 15000000 INFO @ Fri, 10 Dec 2021 15:48:22: 17000000 INFO @ Fri, 10 Dec 2021 15:48:24: 11000000 INFO @ Fri, 10 Dec 2021 15:48:24: 16000000 INFO @ Fri, 10 Dec 2021 15:48:32: 18000000 INFO @ Fri, 10 Dec 2021 15:48:33: 17000000 INFO @ Fri, 10 Dec 2021 15:48:34: 12000000 INFO @ Fri, 10 Dec 2021 15:48:42: 19000000 INFO @ Fri, 10 Dec 2021 15:48:42: 18000000 INFO @ Fri, 10 Dec 2021 15:48:45: 13000000 INFO @ Fri, 10 Dec 2021 15:48:51: 19000000 INFO @ Fri, 10 Dec 2021 15:48:52: 20000000 INFO @ Fri, 10 Dec 2021 15:48:55: 14000000 INFO @ Fri, 10 Dec 2021 15:49:00: 20000000 INFO @ Fri, 10 Dec 2021 15:49:03: 21000000 INFO @ Fri, 10 Dec 2021 15:49:06: 15000000 INFO @ Fri, 10 Dec 2021 15:49:10: 21000000 INFO @ Fri, 10 Dec 2021 15:49:13: 22000000 INFO @ Fri, 10 Dec 2021 15:49:17: 16000000 INFO @ Fri, 10 Dec 2021 15:49:19: 22000000 INFO @ Fri, 10 Dec 2021 15:49:23: 23000000 INFO @ Fri, 10 Dec 2021 15:49:27: 17000000 INFO @ Fri, 10 Dec 2021 15:49:28: 23000000 INFO @ Fri, 10 Dec 2021 15:49:33: 24000000 INFO @ Fri, 10 Dec 2021 15:49:37: 24000000 INFO @ Fri, 10 Dec 2021 15:49:37: 18000000 INFO @ Fri, 10 Dec 2021 15:49:44: 25000000 INFO @ Fri, 10 Dec 2021 15:49:46: 25000000 INFO @ Fri, 10 Dec 2021 15:49:48: 19000000 INFO @ Fri, 10 Dec 2021 15:49:54: 26000000 INFO @ Fri, 10 Dec 2021 15:49:55: 26000000 INFO @ Fri, 10 Dec 2021 15:49:58: 20000000 INFO @ Fri, 10 Dec 2021 15:50:04: 27000000 INFO @ Fri, 10 Dec 2021 15:50:05: 27000000 INFO @ Fri, 10 Dec 2021 15:50:08: 21000000 INFO @ Fri, 10 Dec 2021 15:50:15: 28000000 INFO @ Fri, 10 Dec 2021 15:50:15: 28000000 INFO @ Fri, 10 Dec 2021 15:50:19: 22000000 INFO @ Fri, 10 Dec 2021 15:50:25: 29000000 INFO @ Fri, 10 Dec 2021 15:50:26: 29000000 INFO @ Fri, 10 Dec 2021 15:50:29: 23000000 INFO @ Fri, 10 Dec 2021 15:50:35: 30000000 INFO @ Fri, 10 Dec 2021 15:50:36: 30000000 INFO @ Fri, 10 Dec 2021 15:50:39: 24000000 INFO @ Fri, 10 Dec 2021 15:50:46: 31000000 INFO @ Fri, 10 Dec 2021 15:50:47: 31000000 INFO @ Fri, 10 Dec 2021 15:50:49: 25000000 INFO @ Fri, 10 Dec 2021 15:50:57: 32000000 INFO @ Fri, 10 Dec 2021 15:50:58: 32000000 INFO @ Fri, 10 Dec 2021 15:50:59: 26000000 INFO @ Fri, 10 Dec 2021 15:51:07: 33000000 INFO @ Fri, 10 Dec 2021 15:51:08: 33000000 INFO @ Fri, 10 Dec 2021 15:51:10: 27000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 15:51:18: 34000000 INFO @ Fri, 10 Dec 2021 15:51:18: 34000000 INFO @ Fri, 10 Dec 2021 15:51:20: 28000000 INFO @ Fri, 10 Dec 2021 15:51:28: 35000000 INFO @ Fri, 10 Dec 2021 15:51:29: 35000000 INFO @ Fri, 10 Dec 2021 15:51:30: 29000000 INFO @ Fri, 10 Dec 2021 15:51:39: 36000000 INFO @ Fri, 10 Dec 2021 15:51:40: 36000000 INFO @ Fri, 10 Dec 2021 15:51:41: 30000000 INFO @ Fri, 10 Dec 2021 15:51:50: 37000000 INFO @ Fri, 10 Dec 2021 15:51:51: 37000000 INFO @ Fri, 10 Dec 2021 15:51:51: 31000000 INFO @ Fri, 10 Dec 2021 15:52:01: 38000000 INFO @ Fri, 10 Dec 2021 15:52:01: 32000000 INFO @ Fri, 10 Dec 2021 15:52:02: 38000000 INFO @ Fri, 10 Dec 2021 15:52:12: 33000000 INFO @ Fri, 10 Dec 2021 15:52:12: 39000000 INFO @ Fri, 10 Dec 2021 15:52:13: 39000000 INFO @ Fri, 10 Dec 2021 15:52:22: 34000000 INFO @ Fri, 10 Dec 2021 15:52:23: 40000000 INFO @ Fri, 10 Dec 2021 15:52:24: 40000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 15:52:32: 35000000 INFO @ Fri, 10 Dec 2021 15:52:34: 41000000 INFO @ Fri, 10 Dec 2021 15:52:35: 41000000 INFO @ Fri, 10 Dec 2021 15:52:43: 36000000 INFO @ Fri, 10 Dec 2021 15:52:45: 42000000 INFO @ Fri, 10 Dec 2021 15:52:45: 42000000 INFO @ Fri, 10 Dec 2021 15:52:53: 37000000 INFO @ Fri, 10 Dec 2021 15:52:55: 43000000 INFO @ Fri, 10 Dec 2021 15:52:56: 43000000 INFO @ Fri, 10 Dec 2021 15:53:04: 38000000 INFO @ Fri, 10 Dec 2021 15:53:06: 44000000 INFO @ Fri, 10 Dec 2021 15:53:07: 44000000 INFO @ Fri, 10 Dec 2021 15:53:14: 39000000 INFO @ Fri, 10 Dec 2021 15:53:17: 45000000 INFO @ Fri, 10 Dec 2021 15:53:18: 45000000 INFO @ Fri, 10 Dec 2021 15:53:25: 40000000 INFO @ Fri, 10 Dec 2021 15:53:27: 46000000 INFO @ Fri, 10 Dec 2021 15:53:28: 46000000 INFO @ Fri, 10 Dec 2021 15:53:36: 41000000 INFO @ Fri, 10 Dec 2021 15:53:38: 47000000 INFO @ Fri, 10 Dec 2021 15:53:38: 47000000 INFO @ Fri, 10 Dec 2021 15:53:47: 42000000 INFO @ Fri, 10 Dec 2021 15:53:48: 48000000 INFO @ Fri, 10 Dec 2021 15:53:49: 48000000 INFO @ Fri, 10 Dec 2021 15:53:57: 43000000 INFO @ Fri, 10 Dec 2021 15:53:59: 49000000 INFO @ Fri, 10 Dec 2021 15:53:59: 49000000 INFO @ Fri, 10 Dec 2021 15:54:08: 44000000 INFO @ Fri, 10 Dec 2021 15:54:09: 50000000 INFO @ Fri, 10 Dec 2021 15:54:10: 50000000 INFO @ Fri, 10 Dec 2021 15:54:19: 45000000 INFO @ Fri, 10 Dec 2021 15:54:20: 51000000 INFO @ Fri, 10 Dec 2021 15:54:21: 51000000 INFO @ Fri, 10 Dec 2021 15:54:29: 46000000 INFO @ Fri, 10 Dec 2021 15:54:31: 52000000 INFO @ Fri, 10 Dec 2021 15:54:31: 52000000 INFO @ Fri, 10 Dec 2021 15:54:39: 47000000 INFO @ Fri, 10 Dec 2021 15:54:41: 53000000 INFO @ Fri, 10 Dec 2021 15:54:42: 53000000 INFO @ Fri, 10 Dec 2021 15:54:49: 48000000 INFO @ Fri, 10 Dec 2021 15:54:52: 54000000 INFO @ Fri, 10 Dec 2021 15:54:53: 54000000 INFO @ Fri, 10 Dec 2021 15:54:59: 49000000 INFO @ Fri, 10 Dec 2021 15:55:03: 55000000 INFO @ Fri, 10 Dec 2021 15:55:04: 55000000 INFO @ Fri, 10 Dec 2021 15:55:10: 50000000 INFO @ Fri, 10 Dec 2021 15:55:14: 56000000 INFO @ Fri, 10 Dec 2021 15:55:15: 56000000 INFO @ Fri, 10 Dec 2021 15:55:20: 51000000 INFO @ Fri, 10 Dec 2021 15:55:25: 57000000 INFO @ Fri, 10 Dec 2021 15:55:26: 57000000 INFO @ Fri, 10 Dec 2021 15:55:30: 52000000 INFO @ Fri, 10 Dec 2021 15:55:36: 58000000 INFO @ Fri, 10 Dec 2021 15:55:37: 58000000 INFO @ Fri, 10 Dec 2021 15:55:40: 53000000 INFO @ Fri, 10 Dec 2021 15:55:47: 59000000 INFO @ Fri, 10 Dec 2021 15:55:47: 59000000 INFO @ Fri, 10 Dec 2021 15:55:51: 54000000 INFO @ Fri, 10 Dec 2021 15:55:57: 60000000 INFO @ Fri, 10 Dec 2021 15:55:58: 60000000 INFO @ Fri, 10 Dec 2021 15:56:01: 55000000 INFO @ Fri, 10 Dec 2021 15:56:08: 61000000 INFO @ Fri, 10 Dec 2021 15:56:08: 61000000 INFO @ Fri, 10 Dec 2021 15:56:12: 56000000 INFO @ Fri, 10 Dec 2021 15:56:16: #1 tag size is determined as 118 bps INFO @ Fri, 10 Dec 2021 15:56:16: #1 tag size = 118 INFO @ Fri, 10 Dec 2021 15:56:16: #1 total tags in treatment: 24090988 INFO @ Fri, 10 Dec 2021 15:56:16: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:56:16: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:56:17: #1 tags after filtering in treatment: 17287025 INFO @ Fri, 10 Dec 2021 15:56:17: #1 Redundant rate of treatment: 0.28 INFO @ Fri, 10 Dec 2021 15:56:17: #1 finished! INFO @ Fri, 10 Dec 2021 15:56:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:56:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:56:17: #1 tag size is determined as 118 bps INFO @ Fri, 10 Dec 2021 15:56:17: #1 tag size = 118 INFO @ Fri, 10 Dec 2021 15:56:17: #1 total tags in treatment: 24090988 INFO @ Fri, 10 Dec 2021 15:56:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:56:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:56:17: #1 tags after filtering in treatment: 17287025 INFO @ Fri, 10 Dec 2021 15:56:17: #1 Redundant rate of treatment: 0.28 INFO @ Fri, 10 Dec 2021 15:56:17: #1 finished! INFO @ Fri, 10 Dec 2021 15:56:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:56:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:56:18: #2 number of paired peaks: 2293 INFO @ Fri, 10 Dec 2021 15:56:18: start model_add_line... INFO @ Fri, 10 Dec 2021 15:56:18: start X-correlation... INFO @ Fri, 10 Dec 2021 15:56:18: end of X-cor INFO @ Fri, 10 Dec 2021 15:56:18: #2 finished! INFO @ Fri, 10 Dec 2021 15:56:18: #2 predicted fragment length is 123 bps INFO @ Fri, 10 Dec 2021 15:56:18: #2 alternative fragment length(s) may be 123 bps INFO @ Fri, 10 Dec 2021 15:56:18: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.05_model.r WARNING @ Fri, 10 Dec 2021 15:56:18: #2 Since the d (123) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:56:18: #2 You may need to consider one of the other alternative d(s): 123 WARNING @ Fri, 10 Dec 2021 15:56:18: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:56:18: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:56:18: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:56:18: #2 number of paired peaks: 2293 INFO @ Fri, 10 Dec 2021 15:56:18: start model_add_line... INFO @ Fri, 10 Dec 2021 15:56:19: start X-correlation... INFO @ Fri, 10 Dec 2021 15:56:19: end of X-cor INFO @ Fri, 10 Dec 2021 15:56:19: #2 finished! INFO @ Fri, 10 Dec 2021 15:56:19: #2 predicted fragment length is 123 bps INFO @ Fri, 10 Dec 2021 15:56:19: #2 alternative fragment length(s) may be 123 bps INFO @ Fri, 10 Dec 2021 15:56:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.10_model.r WARNING @ Fri, 10 Dec 2021 15:56:19: #2 Since the d (123) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:56:19: #2 You may need to consider one of the other alternative d(s): 123 WARNING @ Fri, 10 Dec 2021 15:56:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:56:19: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:56:19: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:56:22: 57000000 INFO @ Fri, 10 Dec 2021 15:56:32: 58000000 INFO @ Fri, 10 Dec 2021 15:56:41: 59000000 INFO @ Fri, 10 Dec 2021 15:56:49: 60000000 INFO @ Fri, 10 Dec 2021 15:56:54: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:56:56: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:56:58: 61000000 INFO @ Fri, 10 Dec 2021 15:57:05: #1 tag size is determined as 118 bps INFO @ Fri, 10 Dec 2021 15:57:05: #1 tag size = 118 INFO @ Fri, 10 Dec 2021 15:57:05: #1 total tags in treatment: 24090988 INFO @ Fri, 10 Dec 2021 15:57:05: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:57:05: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:57:05: #1 tags after filtering in treatment: 17287025 INFO @ Fri, 10 Dec 2021 15:57:05: #1 Redundant rate of treatment: 0.28 INFO @ Fri, 10 Dec 2021 15:57:05: #1 finished! INFO @ Fri, 10 Dec 2021 15:57:05: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:57:05: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:57:07: #2 number of paired peaks: 2293 INFO @ Fri, 10 Dec 2021 15:57:07: start model_add_line... INFO @ Fri, 10 Dec 2021 15:57:07: start X-correlation... INFO @ Fri, 10 Dec 2021 15:57:07: end of X-cor INFO @ Fri, 10 Dec 2021 15:57:07: #2 finished! INFO @ Fri, 10 Dec 2021 15:57:07: #2 predicted fragment length is 123 bps INFO @ Fri, 10 Dec 2021 15:57:07: #2 alternative fragment length(s) may be 123 bps INFO @ Fri, 10 Dec 2021 15:57:07: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.20_model.r WARNING @ Fri, 10 Dec 2021 15:57:07: #2 Since the d (123) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:57:07: #2 You may need to consider one of the other alternative d(s): 123 WARNING @ Fri, 10 Dec 2021 15:57:07: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:57:07: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:57:07: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:57:12: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.10_peaks.xls INFO @ Fri, 10 Dec 2021 15:57:13: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:57:13: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.10_summits.bed INFO @ Fri, 10 Dec 2021 15:57:13: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (10811 records, 4 fields): 11 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:57:15: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.05_peaks.xls INFO @ Fri, 10 Dec 2021 15:57:15: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:57:15: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.05_summits.bed INFO @ Fri, 10 Dec 2021 15:57:15: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (15149 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:57:43: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:58:02: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.20_peaks.xls INFO @ Fri, 10 Dec 2021 15:58:02: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:58:02: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978805/ERX3978805.20_summits.bed INFO @ Fri, 10 Dec 2021 15:58:02: Done! pass1 - making usageList (14 chroms): 2 millis pass2 - checking and writing primary data (6250 records, 4 fields): 7 millis CompletedMACS2peakCalling