Job ID = 14167763 SRX = ERX3978803 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 19617105 spots for ERR3975840/ERR3975840.sra Written 19617105 spots for ERR3975840/ERR3975840.sra Read 24626302 spots for ERR3975893/ERR3975893.sra Written 24626302 spots for ERR3975893/ERR3975893.sra fastq に変換しました。 bowtie でマッピング中... Your job 14168788 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:27:55 44243407 reads; of these: 44243407 (100.00%) were paired; of these: 11817010 (26.71%) aligned concordantly 0 times 20930447 (47.31%) aligned concordantly exactly 1 time 11495950 (25.98%) aligned concordantly >1 times ---- 11817010 pairs aligned concordantly 0 times; of these: 4248461 (35.95%) aligned discordantly 1 time ---- 7568549 pairs aligned 0 times concordantly or discordantly; of these: 15137098 mates make up the pairs; of these: 9406170 (62.14%) aligned 0 times 762074 (5.03%) aligned exactly 1 time 4968854 (32.83%) aligned >1 times 89.37% overall alignment rate Time searching: 01:27:55 Overall time: 01:27:55 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 44 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 11205892 / 36235907 = 0.3092 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:25:53: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:25:53: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:25:53: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:26:03: 1000000 INFO @ Fri, 10 Dec 2021 15:26:11: 2000000 INFO @ Fri, 10 Dec 2021 15:26:19: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:26:21: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:26:21: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:26:21: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:26:28: 4000000 INFO @ Fri, 10 Dec 2021 15:26:32: 1000000 INFO @ Fri, 10 Dec 2021 15:26:37: 5000000 INFO @ Fri, 10 Dec 2021 15:26:43: 2000000 INFO @ Fri, 10 Dec 2021 15:26:46: 6000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Fri, 10 Dec 2021 15:26:51: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Fri, 10 Dec 2021 15:26:51: #1 read tag files... INFO @ Fri, 10 Dec 2021 15:26:51: #1 read treatment tags... INFO @ Fri, 10 Dec 2021 15:26:54: 3000000 INFO @ Fri, 10 Dec 2021 15:26:56: 7000000 INFO @ Fri, 10 Dec 2021 15:27:03: 1000000 INFO @ Fri, 10 Dec 2021 15:27:05: 8000000 INFO @ Fri, 10 Dec 2021 15:27:05: 4000000 INFO @ Fri, 10 Dec 2021 15:27:14: 9000000 INFO @ Fri, 10 Dec 2021 15:27:14: 2000000 INFO @ Fri, 10 Dec 2021 15:27:16: 5000000 INFO @ Fri, 10 Dec 2021 15:27:23: 10000000 INFO @ Fri, 10 Dec 2021 15:27:25: 3000000 INFO @ Fri, 10 Dec 2021 15:27:28: 6000000 INFO @ Fri, 10 Dec 2021 15:27:32: 11000000 INFO @ Fri, 10 Dec 2021 15:27:37: 4000000 INFO @ Fri, 10 Dec 2021 15:27:39: 7000000 INFO @ Fri, 10 Dec 2021 15:27:42: 12000000 INFO @ Fri, 10 Dec 2021 15:27:48: 5000000 INFO @ Fri, 10 Dec 2021 15:27:50: 8000000 INFO @ Fri, 10 Dec 2021 15:27:51: 13000000 INFO @ Fri, 10 Dec 2021 15:27:59: 6000000 INFO @ Fri, 10 Dec 2021 15:28:00: 14000000 INFO @ Fri, 10 Dec 2021 15:28:01: 9000000 INFO @ Fri, 10 Dec 2021 15:28:10: 15000000 INFO @ Fri, 10 Dec 2021 15:28:11: 7000000 INFO @ Fri, 10 Dec 2021 15:28:12: 10000000 INFO @ Fri, 10 Dec 2021 15:28:19: 16000000 INFO @ Fri, 10 Dec 2021 15:28:22: 8000000 INFO @ Fri, 10 Dec 2021 15:28:24: 11000000 INFO @ Fri, 10 Dec 2021 15:28:29: 17000000 INFO @ Fri, 10 Dec 2021 15:28:33: 9000000 INFO @ Fri, 10 Dec 2021 15:28:35: 12000000 INFO @ Fri, 10 Dec 2021 15:28:38: 18000000 INFO @ Fri, 10 Dec 2021 15:28:44: 10000000 INFO @ Fri, 10 Dec 2021 15:28:46: 13000000 INFO @ Fri, 10 Dec 2021 15:28:47: 19000000 INFO @ Fri, 10 Dec 2021 15:28:56: 11000000 INFO @ Fri, 10 Dec 2021 15:28:57: 20000000 INFO @ Fri, 10 Dec 2021 15:28:58: 14000000 INFO @ Fri, 10 Dec 2021 15:29:06: 21000000 INFO @ Fri, 10 Dec 2021 15:29:07: 12000000 INFO @ Fri, 10 Dec 2021 15:29:09: 15000000 INFO @ Fri, 10 Dec 2021 15:29:15: 22000000 INFO @ Fri, 10 Dec 2021 15:29:18: 13000000 INFO @ Fri, 10 Dec 2021 15:29:20: 16000000 INFO @ Fri, 10 Dec 2021 15:29:25: 23000000 INFO @ Fri, 10 Dec 2021 15:29:29: 14000000 INFO @ Fri, 10 Dec 2021 15:29:32: 17000000 INFO @ Fri, 10 Dec 2021 15:29:34: 24000000 INFO @ Fri, 10 Dec 2021 15:29:41: 15000000 INFO @ Fri, 10 Dec 2021 15:29:43: 18000000 INFO @ Fri, 10 Dec 2021 15:29:43: 25000000 INFO @ Fri, 10 Dec 2021 15:29:52: 16000000 INFO @ Fri, 10 Dec 2021 15:29:52: 26000000 INFO @ Fri, 10 Dec 2021 15:29:54: 19000000 INFO @ Fri, 10 Dec 2021 15:30:02: 27000000 INFO @ Fri, 10 Dec 2021 15:30:03: 17000000 INFO @ Fri, 10 Dec 2021 15:30:05: 20000000 INFO @ Fri, 10 Dec 2021 15:30:11: 28000000 INFO @ Fri, 10 Dec 2021 15:30:14: 18000000 INFO @ Fri, 10 Dec 2021 15:30:17: 21000000 INFO @ Fri, 10 Dec 2021 15:30:20: 29000000 INFO @ Fri, 10 Dec 2021 15:30:26: 19000000 INFO @ Fri, 10 Dec 2021 15:30:28: 22000000 INFO @ Fri, 10 Dec 2021 15:30:30: 30000000 INFO @ Fri, 10 Dec 2021 15:30:37: 20000000 INFO @ Fri, 10 Dec 2021 15:30:39: 31000000 INFO @ Fri, 10 Dec 2021 15:30:39: 23000000 INFO @ Fri, 10 Dec 2021 15:30:48: 21000000 INFO @ Fri, 10 Dec 2021 15:30:48: 32000000 INFO @ Fri, 10 Dec 2021 15:30:50: 24000000 INFO @ Fri, 10 Dec 2021 15:30:58: 33000000 INFO @ Fri, 10 Dec 2021 15:31:00: 22000000 INFO @ Fri, 10 Dec 2021 15:31:02: 25000000 INFO @ Fri, 10 Dec 2021 15:31:07: 34000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Fri, 10 Dec 2021 15:31:11: 23000000 INFO @ Fri, 10 Dec 2021 15:31:13: 26000000 INFO @ Fri, 10 Dec 2021 15:31:16: 35000000 INFO @ Fri, 10 Dec 2021 15:31:22: 24000000 INFO @ Fri, 10 Dec 2021 15:31:24: 27000000 INFO @ Fri, 10 Dec 2021 15:31:25: 36000000 INFO @ Fri, 10 Dec 2021 15:31:33: 25000000 INFO @ Fri, 10 Dec 2021 15:31:34: 37000000 INFO @ Fri, 10 Dec 2021 15:31:35: 28000000 INFO @ Fri, 10 Dec 2021 15:31:43: 38000000 INFO @ Fri, 10 Dec 2021 15:31:45: 26000000 INFO @ Fri, 10 Dec 2021 15:31:47: 29000000 INFO @ Fri, 10 Dec 2021 15:31:52: 39000000 INFO @ Fri, 10 Dec 2021 15:31:56: 27000000 INFO @ Fri, 10 Dec 2021 15:31:58: 30000000 INFO @ Fri, 10 Dec 2021 15:32:01: 40000000 INFO @ Fri, 10 Dec 2021 15:32:07: 28000000 INFO @ Fri, 10 Dec 2021 15:32:09: 31000000 BigWig に変換しました。 INFO @ Fri, 10 Dec 2021 15:32:10: 41000000 INFO @ Fri, 10 Dec 2021 15:32:18: 29000000 INFO @ Fri, 10 Dec 2021 15:32:20: 32000000 INFO @ Fri, 10 Dec 2021 15:32:20: 42000000 INFO @ Fri, 10 Dec 2021 15:32:29: 30000000 INFO @ Fri, 10 Dec 2021 15:32:30: 43000000 INFO @ Fri, 10 Dec 2021 15:32:32: 33000000 INFO @ Fri, 10 Dec 2021 15:32:40: 44000000 INFO @ Fri, 10 Dec 2021 15:32:41: 31000000 INFO @ Fri, 10 Dec 2021 15:32:43: 34000000 INFO @ Fri, 10 Dec 2021 15:32:51: 45000000 INFO @ Fri, 10 Dec 2021 15:32:52: 32000000 INFO @ Fri, 10 Dec 2021 15:32:54: 35000000 INFO @ Fri, 10 Dec 2021 15:33:01: 46000000 INFO @ Fri, 10 Dec 2021 15:33:03: 33000000 INFO @ Fri, 10 Dec 2021 15:33:06: 36000000 INFO @ Fri, 10 Dec 2021 15:33:12: 47000000 INFO @ Fri, 10 Dec 2021 15:33:15: 34000000 INFO @ Fri, 10 Dec 2021 15:33:16: 37000000 INFO @ Fri, 10 Dec 2021 15:33:22: 48000000 INFO @ Fri, 10 Dec 2021 15:33:25: 35000000 INFO @ Fri, 10 Dec 2021 15:33:27: 38000000 INFO @ Fri, 10 Dec 2021 15:33:33: 49000000 INFO @ Fri, 10 Dec 2021 15:33:36: 36000000 INFO @ Fri, 10 Dec 2021 15:33:38: 39000000 INFO @ Fri, 10 Dec 2021 15:33:44: 50000000 INFO @ Fri, 10 Dec 2021 15:33:47: 37000000 INFO @ Fri, 10 Dec 2021 15:33:48: 40000000 INFO @ Fri, 10 Dec 2021 15:33:54: 51000000 INFO @ Fri, 10 Dec 2021 15:33:58: 38000000 INFO @ Fri, 10 Dec 2021 15:33:59: 41000000 INFO @ Fri, 10 Dec 2021 15:34:05: 52000000 INFO @ Fri, 10 Dec 2021 15:34:08: 42000000 INFO @ Fri, 10 Dec 2021 15:34:08: 39000000 INFO @ Fri, 10 Dec 2021 15:34:15: 53000000 INFO @ Fri, 10 Dec 2021 15:34:17: 43000000 INFO @ Fri, 10 Dec 2021 15:34:19: 40000000 INFO @ Fri, 10 Dec 2021 15:34:26: 54000000 INFO @ Fri, 10 Dec 2021 15:34:26: 44000000 INFO @ Fri, 10 Dec 2021 15:34:29: 41000000 INFO @ Fri, 10 Dec 2021 15:34:35: 45000000 INFO @ Fri, 10 Dec 2021 15:34:36: 55000000 INFO @ Fri, 10 Dec 2021 15:34:38: 42000000 INFO @ Fri, 10 Dec 2021 15:34:44: 46000000 INFO @ Fri, 10 Dec 2021 15:34:47: 56000000 INFO @ Fri, 10 Dec 2021 15:34:48: 43000000 INFO @ Fri, 10 Dec 2021 15:34:54: #1 tag size is determined as 118 bps INFO @ Fri, 10 Dec 2021 15:34:54: #1 tag size = 118 INFO @ Fri, 10 Dec 2021 15:34:54: #1 total tags in treatment: 21769145 INFO @ Fri, 10 Dec 2021 15:34:54: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:34:54: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:34:54: 47000000 INFO @ Fri, 10 Dec 2021 15:34:54: #1 tags after filtering in treatment: 14821228 INFO @ Fri, 10 Dec 2021 15:34:54: #1 Redundant rate of treatment: 0.32 INFO @ Fri, 10 Dec 2021 15:34:54: #1 finished! INFO @ Fri, 10 Dec 2021 15:34:54: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:34:54: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:34:55: #2 number of paired peaks: 3724 INFO @ Fri, 10 Dec 2021 15:34:55: start model_add_line... INFO @ Fri, 10 Dec 2021 15:34:55: start X-correlation... INFO @ Fri, 10 Dec 2021 15:34:55: end of X-cor INFO @ Fri, 10 Dec 2021 15:34:55: #2 finished! INFO @ Fri, 10 Dec 2021 15:34:55: #2 predicted fragment length is 128 bps INFO @ Fri, 10 Dec 2021 15:34:55: #2 alternative fragment length(s) may be 128 bps INFO @ Fri, 10 Dec 2021 15:34:55: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.05_model.r WARNING @ Fri, 10 Dec 2021 15:34:55: #2 Since the d (128) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:34:55: #2 You may need to consider one of the other alternative d(s): 128 WARNING @ Fri, 10 Dec 2021 15:34:55: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:34:55: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:34:55: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:34:56: 44000000 INFO @ Fri, 10 Dec 2021 15:35:03: 48000000 INFO @ Fri, 10 Dec 2021 15:35:05: 45000000 INFO @ Fri, 10 Dec 2021 15:35:11: 49000000 INFO @ Fri, 10 Dec 2021 15:35:14: 46000000 INFO @ Fri, 10 Dec 2021 15:35:20: 50000000 INFO @ Fri, 10 Dec 2021 15:35:23: 47000000 INFO @ Fri, 10 Dec 2021 15:35:27: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:35:29: 51000000 INFO @ Fri, 10 Dec 2021 15:35:31: 48000000 INFO @ Fri, 10 Dec 2021 15:35:37: 52000000 INFO @ Fri, 10 Dec 2021 15:35:40: 49000000 INFO @ Fri, 10 Dec 2021 15:35:43: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.05_peaks.xls INFO @ Fri, 10 Dec 2021 15:35:44: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.05_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:35:44: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.05_summits.bed INFO @ Fri, 10 Dec 2021 15:35:44: Done! pass1 - making usageList (15 chroms): 3 millis pass2 - checking and writing primary data (15164 records, 4 fields): 15 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:35:46: 53000000 INFO @ Fri, 10 Dec 2021 15:35:48: 50000000 INFO @ Fri, 10 Dec 2021 15:35:54: 54000000 INFO @ Fri, 10 Dec 2021 15:35:57: 51000000 INFO @ Fri, 10 Dec 2021 15:36:03: 55000000 INFO @ Fri, 10 Dec 2021 15:36:05: 52000000 INFO @ Fri, 10 Dec 2021 15:36:11: 56000000 INFO @ Fri, 10 Dec 2021 15:36:14: 53000000 INFO @ Fri, 10 Dec 2021 15:36:17: #1 tag size is determined as 118 bps INFO @ Fri, 10 Dec 2021 15:36:17: #1 tag size = 118 INFO @ Fri, 10 Dec 2021 15:36:17: #1 total tags in treatment: 21769145 INFO @ Fri, 10 Dec 2021 15:36:17: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:36:17: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:36:17: #1 tags after filtering in treatment: 14821228 INFO @ Fri, 10 Dec 2021 15:36:17: #1 Redundant rate of treatment: 0.32 INFO @ Fri, 10 Dec 2021 15:36:17: #1 finished! INFO @ Fri, 10 Dec 2021 15:36:17: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:36:17: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:36:18: #2 number of paired peaks: 3724 INFO @ Fri, 10 Dec 2021 15:36:18: start model_add_line... INFO @ Fri, 10 Dec 2021 15:36:19: start X-correlation... INFO @ Fri, 10 Dec 2021 15:36:19: end of X-cor INFO @ Fri, 10 Dec 2021 15:36:19: #2 finished! INFO @ Fri, 10 Dec 2021 15:36:19: #2 predicted fragment length is 128 bps INFO @ Fri, 10 Dec 2021 15:36:19: #2 alternative fragment length(s) may be 128 bps INFO @ Fri, 10 Dec 2021 15:36:19: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.10_model.r WARNING @ Fri, 10 Dec 2021 15:36:19: #2 Since the d (128) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:36:19: #2 You may need to consider one of the other alternative d(s): 128 WARNING @ Fri, 10 Dec 2021 15:36:19: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:36:19: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:36:19: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:36:22: 54000000 INFO @ Fri, 10 Dec 2021 15:36:29: 55000000 INFO @ Fri, 10 Dec 2021 15:36:37: 56000000 INFO @ Fri, 10 Dec 2021 15:36:42: #1 tag size is determined as 118 bps INFO @ Fri, 10 Dec 2021 15:36:42: #1 tag size = 118 INFO @ Fri, 10 Dec 2021 15:36:42: #1 total tags in treatment: 21769145 INFO @ Fri, 10 Dec 2021 15:36:42: #1 user defined the maximum tags... INFO @ Fri, 10 Dec 2021 15:36:42: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Fri, 10 Dec 2021 15:36:43: #1 tags after filtering in treatment: 14821228 INFO @ Fri, 10 Dec 2021 15:36:43: #1 Redundant rate of treatment: 0.32 INFO @ Fri, 10 Dec 2021 15:36:43: #1 finished! INFO @ Fri, 10 Dec 2021 15:36:43: #2 Build Peak Model... INFO @ Fri, 10 Dec 2021 15:36:43: #2 looking for paired plus/minus strand peaks... INFO @ Fri, 10 Dec 2021 15:36:44: #2 number of paired peaks: 3724 INFO @ Fri, 10 Dec 2021 15:36:44: start model_add_line... INFO @ Fri, 10 Dec 2021 15:36:44: start X-correlation... INFO @ Fri, 10 Dec 2021 15:36:44: end of X-cor INFO @ Fri, 10 Dec 2021 15:36:44: #2 finished! INFO @ Fri, 10 Dec 2021 15:36:44: #2 predicted fragment length is 128 bps INFO @ Fri, 10 Dec 2021 15:36:44: #2 alternative fragment length(s) may be 128 bps INFO @ Fri, 10 Dec 2021 15:36:44: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.20_model.r WARNING @ Fri, 10 Dec 2021 15:36:44: #2 Since the d (128) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Fri, 10 Dec 2021 15:36:44: #2 You may need to consider one of the other alternative d(s): 128 WARNING @ Fri, 10 Dec 2021 15:36:44: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Fri, 10 Dec 2021 15:36:44: #3 Call peaks... INFO @ Fri, 10 Dec 2021 15:36:44: #3 Pre-compute pvalue-qvalue table... INFO @ Fri, 10 Dec 2021 15:36:50: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:37:05: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.10_peaks.xls INFO @ Fri, 10 Dec 2021 15:37:05: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.10_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:37:05: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.10_summits.bed INFO @ Fri, 10 Dec 2021 15:37:05: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (11738 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Fri, 10 Dec 2021 15:37:14: #3 Call peaks for each chromosome... INFO @ Fri, 10 Dec 2021 15:37:30: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.20_peaks.xls INFO @ Fri, 10 Dec 2021 15:37:30: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.20_peaks.narrowPeak INFO @ Fri, 10 Dec 2021 15:37:30: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978803/ERX3978803.20_summits.bed INFO @ Fri, 10 Dec 2021 15:37:30: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (7536 records, 4 fields): 9 millis CompletedMACS2peakCalling