Job ID = 14169880 SRX = ERX3978793 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... Read 27226374 spots for ERR3975823/ERR3975823.sra Written 27226374 spots for ERR3975823/ERR3975823.sra Read 23450311 spots for ERR3975832/ERR3975832.sra Written 23450311 spots for ERR3975832/ERR3975832.sra fastq に変換しました。 bowtie でマッピング中... Your job 14170698 ("srTdm6") has been submitted Time loading reference: 00:00:00 Time loading forward index: 00:00:01 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:51:07 50676685 reads; of these: 50676685 (100.00%) were paired; of these: 11515962 (22.72%) aligned concordantly 0 times 27552784 (54.37%) aligned concordantly exactly 1 time 11607939 (22.91%) aligned concordantly >1 times ---- 11515962 pairs aligned concordantly 0 times; of these: 4337021 (37.66%) aligned discordantly 1 time ---- 7178941 pairs aligned 0 times concordantly or discordantly; of these: 14357882 mates make up the pairs; of these: 9651703 (67.22%) aligned 0 times 953587 (6.64%) aligned exactly 1 time 3752592 (26.14%) aligned >1 times 90.48% overall alignment rate Time searching: 01:51:08 Overall time: 01:51:08 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 60 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 12775598 / 43069337 = 0.2966 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:18:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:18:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:18:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:18:25: 1000000 INFO @ Sat, 11 Dec 2021 06:18:32: 2000000 INFO @ Sat, 11 Dec 2021 06:18:38: 3000000 INFO @ Sat, 11 Dec 2021 06:18:45: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:18:48: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:18:48: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:18:48: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:18:53: 5000000 INFO @ Sat, 11 Dec 2021 06:18:58: 1000000 INFO @ Sat, 11 Dec 2021 06:19:01: 6000000 INFO @ Sat, 11 Dec 2021 06:19:08: 2000000 INFO @ Sat, 11 Dec 2021 06:19:10: 7000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.8.3/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Sat, 11 Dec 2021 06:19:18: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sat, 11 Dec 2021 06:19:18: #1 read tag files... INFO @ Sat, 11 Dec 2021 06:19:18: #1 read treatment tags... INFO @ Sat, 11 Dec 2021 06:19:18: 8000000 INFO @ Sat, 11 Dec 2021 06:19:18: 3000000 INFO @ Sat, 11 Dec 2021 06:19:27: 9000000 INFO @ Sat, 11 Dec 2021 06:19:27: 1000000 INFO @ Sat, 11 Dec 2021 06:19:28: 4000000 INFO @ Sat, 11 Dec 2021 06:19:35: 10000000 INFO @ Sat, 11 Dec 2021 06:19:37: 2000000 INFO @ Sat, 11 Dec 2021 06:19:38: 5000000 INFO @ Sat, 11 Dec 2021 06:19:44: 11000000 INFO @ Sat, 11 Dec 2021 06:19:47: 6000000 INFO @ Sat, 11 Dec 2021 06:19:47: 3000000 INFO @ Sat, 11 Dec 2021 06:19:53: 12000000 INFO @ Sat, 11 Dec 2021 06:19:57: 7000000 INFO @ Sat, 11 Dec 2021 06:19:57: 4000000 INFO @ Sat, 11 Dec 2021 06:20:01: 13000000 INFO @ Sat, 11 Dec 2021 06:20:06: 8000000 INFO @ Sat, 11 Dec 2021 06:20:07: 5000000 INFO @ Sat, 11 Dec 2021 06:20:10: 14000000 INFO @ Sat, 11 Dec 2021 06:20:16: 9000000 INFO @ Sat, 11 Dec 2021 06:20:17: 6000000 INFO @ Sat, 11 Dec 2021 06:20:18: 15000000 INFO @ Sat, 11 Dec 2021 06:20:26: 10000000 INFO @ Sat, 11 Dec 2021 06:20:27: 7000000 INFO @ Sat, 11 Dec 2021 06:20:27: 16000000 INFO @ Sat, 11 Dec 2021 06:20:36: 11000000 INFO @ Sat, 11 Dec 2021 06:20:36: 17000000 INFO @ Sat, 11 Dec 2021 06:20:36: 8000000 INFO @ Sat, 11 Dec 2021 06:20:44: 18000000 INFO @ Sat, 11 Dec 2021 06:20:45: 12000000 INFO @ Sat, 11 Dec 2021 06:20:46: 9000000 INFO @ Sat, 11 Dec 2021 06:20:53: 19000000 INFO @ Sat, 11 Dec 2021 06:20:55: 13000000 INFO @ Sat, 11 Dec 2021 06:20:56: 10000000 INFO @ Sat, 11 Dec 2021 06:21:02: 20000000 INFO @ Sat, 11 Dec 2021 06:21:05: 14000000 INFO @ Sat, 11 Dec 2021 06:21:06: 11000000 INFO @ Sat, 11 Dec 2021 06:21:10: 21000000 INFO @ Sat, 11 Dec 2021 06:21:15: 15000000 INFO @ Sat, 11 Dec 2021 06:21:15: 12000000 INFO @ Sat, 11 Dec 2021 06:21:19: 22000000 INFO @ Sat, 11 Dec 2021 06:21:24: 16000000 INFO @ Sat, 11 Dec 2021 06:21:25: 13000000 INFO @ Sat, 11 Dec 2021 06:21:27: 23000000 INFO @ Sat, 11 Dec 2021 06:21:34: 17000000 INFO @ Sat, 11 Dec 2021 06:21:35: 14000000 INFO @ Sat, 11 Dec 2021 06:21:36: 24000000 INFO @ Sat, 11 Dec 2021 06:21:43: 18000000 INFO @ Sat, 11 Dec 2021 06:21:45: 25000000 INFO @ Sat, 11 Dec 2021 06:21:45: 15000000 INFO @ Sat, 11 Dec 2021 06:21:53: 19000000 INFO @ Sat, 11 Dec 2021 06:21:53: 26000000 INFO @ Sat, 11 Dec 2021 06:21:55: 16000000 INFO @ Sat, 11 Dec 2021 06:22:02: 27000000 INFO @ Sat, 11 Dec 2021 06:22:03: 20000000 INFO @ Sat, 11 Dec 2021 06:22:04: 17000000 INFO @ Sat, 11 Dec 2021 06:22:11: 28000000 INFO @ Sat, 11 Dec 2021 06:22:13: 21000000 INFO @ Sat, 11 Dec 2021 06:22:14: 18000000 INFO @ Sat, 11 Dec 2021 06:22:19: 29000000 INFO @ Sat, 11 Dec 2021 06:22:23: 22000000 INFO @ Sat, 11 Dec 2021 06:22:24: 19000000 INFO @ Sat, 11 Dec 2021 06:22:28: 30000000 INFO @ Sat, 11 Dec 2021 06:22:33: 23000000 INFO @ Sat, 11 Dec 2021 06:22:34: 20000000 INFO @ Sat, 11 Dec 2021 06:22:36: 31000000 INFO @ Sat, 11 Dec 2021 06:22:44: 21000000 INFO @ Sat, 11 Dec 2021 06:22:44: 24000000 INFO @ Sat, 11 Dec 2021 06:22:45: 32000000 INFO @ Sat, 11 Dec 2021 06:22:53: 22000000 INFO @ Sat, 11 Dec 2021 06:22:53: 33000000 INFO @ Sat, 11 Dec 2021 06:22:54: 25000000 INFO @ Sat, 11 Dec 2021 06:23:02: 34000000 INFO @ Sat, 11 Dec 2021 06:23:03: 23000000 INFO @ Sat, 11 Dec 2021 06:23:04: 26000000 INFO @ Sat, 11 Dec 2021 06:23:11: 35000000 INFO @ Sat, 11 Dec 2021 06:23:13: 24000000 INFO @ Sat, 11 Dec 2021 06:23:15: 27000000 INFO @ Sat, 11 Dec 2021 06:23:19: 36000000 INFO @ Sat, 11 Dec 2021 06:23:23: 25000000 INFO @ Sat, 11 Dec 2021 06:23:25: 28000000 INFO @ Sat, 11 Dec 2021 06:23:28: 37000000 INFO @ Sat, 11 Dec 2021 06:23:33: 26000000 INFO @ Sat, 11 Dec 2021 06:23:35: 29000000 INFO @ Sat, 11 Dec 2021 06:23:36: 38000000 INFO @ Sat, 11 Dec 2021 06:23:42: 27000000 INFO @ Sat, 11 Dec 2021 06:23:45: 39000000 INFO @ Sat, 11 Dec 2021 06:23:45: 30000000 INFO @ Sat, 11 Dec 2021 06:23:52: 28000000 INFO @ Sat, 11 Dec 2021 06:23:54: 40000000 INFO @ Sat, 11 Dec 2021 06:23:56: 31000000 INFO @ Sat, 11 Dec 2021 06:24:02: 41000000 INFO @ Sat, 11 Dec 2021 06:24:02: 29000000 INFO @ Sat, 11 Dec 2021 06:24:06: 32000000 INFO @ Sat, 11 Dec 2021 06:24:11: 42000000 INFO @ Sat, 11 Dec 2021 06:24:12: 30000000 INFO @ Sat, 11 Dec 2021 06:24:16: 33000000 INFO @ Sat, 11 Dec 2021 06:24:19: 43000000 INFO @ Sat, 11 Dec 2021 06:24:22: 31000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Sat, 11 Dec 2021 06:24:26: 34000000 INFO @ Sat, 11 Dec 2021 06:24:28: 44000000 INFO @ Sat, 11 Dec 2021 06:24:31: 32000000 INFO @ Sat, 11 Dec 2021 06:24:36: 35000000 INFO @ Sat, 11 Dec 2021 06:24:36: 45000000 INFO @ Sat, 11 Dec 2021 06:24:41: 33000000 INFO @ Sat, 11 Dec 2021 06:24:45: 46000000 INFO @ Sat, 11 Dec 2021 06:24:47: 36000000 INFO @ Sat, 11 Dec 2021 06:24:51: 34000000 INFO @ Sat, 11 Dec 2021 06:24:53: 47000000 INFO @ Sat, 11 Dec 2021 06:24:57: 37000000 INFO @ Sat, 11 Dec 2021 06:25:01: 35000000 INFO @ Sat, 11 Dec 2021 06:25:02: 48000000 INFO @ Sat, 11 Dec 2021 06:25:07: 38000000 INFO @ Sat, 11 Dec 2021 06:25:10: 49000000 INFO @ Sat, 11 Dec 2021 06:25:10: 36000000 INFO @ Sat, 11 Dec 2021 06:25:17: 39000000 INFO @ Sat, 11 Dec 2021 06:25:19: 50000000 INFO @ Sat, 11 Dec 2021 06:25:20: 37000000 INFO @ Sat, 11 Dec 2021 06:25:27: 51000000 INFO @ Sat, 11 Dec 2021 06:25:28: 40000000 INFO @ Sat, 11 Dec 2021 06:25:30: 38000000 INFO @ Sat, 11 Dec 2021 06:25:36: 52000000 INFO @ Sat, 11 Dec 2021 06:25:38: 41000000 INFO @ Sat, 11 Dec 2021 06:25:40: 39000000 BigWig に変換しました。 INFO @ Sat, 11 Dec 2021 06:25:45: 53000000 INFO @ Sat, 11 Dec 2021 06:25:48: 42000000 INFO @ Sat, 11 Dec 2021 06:25:49: 40000000 INFO @ Sat, 11 Dec 2021 06:25:53: 54000000 INFO @ Sat, 11 Dec 2021 06:25:59: 43000000 INFO @ Sat, 11 Dec 2021 06:25:59: 41000000 INFO @ Sat, 11 Dec 2021 06:26:02: 55000000 INFO @ Sat, 11 Dec 2021 06:26:09: 42000000 INFO @ Sat, 11 Dec 2021 06:26:09: 44000000 INFO @ Sat, 11 Dec 2021 06:26:11: 56000000 INFO @ Sat, 11 Dec 2021 06:26:18: 43000000 INFO @ Sat, 11 Dec 2021 06:26:19: 45000000 INFO @ Sat, 11 Dec 2021 06:26:19: 57000000 INFO @ Sat, 11 Dec 2021 06:26:28: 44000000 INFO @ Sat, 11 Dec 2021 06:26:28: 58000000 INFO @ Sat, 11 Dec 2021 06:26:29: 46000000 INFO @ Sat, 11 Dec 2021 06:26:37: 59000000 INFO @ Sat, 11 Dec 2021 06:26:37: 45000000 INFO @ Sat, 11 Dec 2021 06:26:39: 47000000 INFO @ Sat, 11 Dec 2021 06:26:45: 60000000 INFO @ Sat, 11 Dec 2021 06:26:47: 46000000 INFO @ Sat, 11 Dec 2021 06:26:49: 48000000 INFO @ Sat, 11 Dec 2021 06:26:54: 61000000 INFO @ Sat, 11 Dec 2021 06:26:56: 47000000 INFO @ Sat, 11 Dec 2021 06:27:00: 49000000 INFO @ Sat, 11 Dec 2021 06:27:03: 62000000 INFO @ Sat, 11 Dec 2021 06:27:05: 48000000 INFO @ Sat, 11 Dec 2021 06:27:10: 50000000 INFO @ Sat, 11 Dec 2021 06:27:11: 63000000 INFO @ Sat, 11 Dec 2021 06:27:15: 49000000 INFO @ Sat, 11 Dec 2021 06:27:20: 64000000 INFO @ Sat, 11 Dec 2021 06:27:20: 51000000 INFO @ Sat, 11 Dec 2021 06:27:24: 50000000 INFO @ Sat, 11 Dec 2021 06:27:29: 65000000 INFO @ Sat, 11 Dec 2021 06:27:31: 52000000 INFO @ Sat, 11 Dec 2021 06:27:34: 51000000 INFO @ Sat, 11 Dec 2021 06:27:37: 66000000 INFO @ Sat, 11 Dec 2021 06:27:39: #1 tag size is determined as 116 bps INFO @ Sat, 11 Dec 2021 06:27:39: #1 tag size = 116 INFO @ Sat, 11 Dec 2021 06:27:39: #1 total tags in treatment: 27067834 INFO @ Sat, 11 Dec 2021 06:27:39: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:27:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:27:39: #1 tags after filtering in treatment: 20270983 INFO @ Sat, 11 Dec 2021 06:27:39: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 06:27:39: #1 finished! INFO @ Sat, 11 Dec 2021 06:27:39: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:27:39: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:27:41: #2 number of paired peaks: 963 WARNING @ Sat, 11 Dec 2021 06:27:41: Fewer paired peaks (963) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 963 pairs to build model! INFO @ Sat, 11 Dec 2021 06:27:41: start model_add_line... INFO @ Sat, 11 Dec 2021 06:27:41: start X-correlation... INFO @ Sat, 11 Dec 2021 06:27:41: end of X-cor INFO @ Sat, 11 Dec 2021 06:27:41: #2 finished! INFO @ Sat, 11 Dec 2021 06:27:41: #2 predicted fragment length is 123 bps INFO @ Sat, 11 Dec 2021 06:27:41: #2 alternative fragment length(s) may be 123 bps INFO @ Sat, 11 Dec 2021 06:27:41: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.05_model.r WARNING @ Sat, 11 Dec 2021 06:27:41: #2 Since the d (123) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:27:41: #2 You may need to consider one of the other alternative d(s): 123 WARNING @ Sat, 11 Dec 2021 06:27:41: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:27:41: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:27:41: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:27:41: 53000000 INFO @ Sat, 11 Dec 2021 06:27:44: 52000000 INFO @ Sat, 11 Dec 2021 06:27:51: 54000000 INFO @ Sat, 11 Dec 2021 06:27:53: 53000000 INFO @ Sat, 11 Dec 2021 06:28:01: 55000000 INFO @ Sat, 11 Dec 2021 06:28:03: 54000000 INFO @ Sat, 11 Dec 2021 06:28:12: 56000000 INFO @ Sat, 11 Dec 2021 06:28:12: 55000000 INFO @ Sat, 11 Dec 2021 06:28:20: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:28:22: 57000000 INFO @ Sat, 11 Dec 2021 06:28:22: 56000000 INFO @ Sat, 11 Dec 2021 06:28:32: 57000000 INFO @ Sat, 11 Dec 2021 06:28:32: 58000000 INFO @ Sat, 11 Dec 2021 06:28:40: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.05_peaks.xls INFO @ Sat, 11 Dec 2021 06:28:40: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.05_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:28:40: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.05_summits.bed INFO @ Sat, 11 Dec 2021 06:28:40: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (12207 records, 4 fields): 12 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:28:42: 58000000 INFO @ Sat, 11 Dec 2021 06:28:42: 59000000 INFO @ Sat, 11 Dec 2021 06:28:52: 59000000 INFO @ Sat, 11 Dec 2021 06:28:52: 60000000 INFO @ Sat, 11 Dec 2021 06:29:01: 60000000 INFO @ Sat, 11 Dec 2021 06:29:02: 61000000 INFO @ Sat, 11 Dec 2021 06:29:11: 61000000 INFO @ Sat, 11 Dec 2021 06:29:11: 62000000 INFO @ Sat, 11 Dec 2021 06:29:21: 62000000 INFO @ Sat, 11 Dec 2021 06:29:21: 63000000 INFO @ Sat, 11 Dec 2021 06:29:30: 63000000 INFO @ Sat, 11 Dec 2021 06:29:31: 64000000 INFO @ Sat, 11 Dec 2021 06:29:40: 64000000 INFO @ Sat, 11 Dec 2021 06:29:40: 65000000 INFO @ Sat, 11 Dec 2021 06:29:50: 65000000 INFO @ Sat, 11 Dec 2021 06:29:50: 66000000 INFO @ Sat, 11 Dec 2021 06:29:52: #1 tag size is determined as 116 bps INFO @ Sat, 11 Dec 2021 06:29:52: #1 tag size = 116 INFO @ Sat, 11 Dec 2021 06:29:52: #1 total tags in treatment: 27067834 INFO @ Sat, 11 Dec 2021 06:29:52: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:29:52: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:29:52: #1 tags after filtering in treatment: 20270983 INFO @ Sat, 11 Dec 2021 06:29:52: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 06:29:52: #1 finished! INFO @ Sat, 11 Dec 2021 06:29:52: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:29:52: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:29:54: #2 number of paired peaks: 963 WARNING @ Sat, 11 Dec 2021 06:29:54: Fewer paired peaks (963) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 963 pairs to build model! INFO @ Sat, 11 Dec 2021 06:29:54: start model_add_line... INFO @ Sat, 11 Dec 2021 06:29:54: start X-correlation... INFO @ Sat, 11 Dec 2021 06:29:54: end of X-cor INFO @ Sat, 11 Dec 2021 06:29:54: #2 finished! INFO @ Sat, 11 Dec 2021 06:29:54: #2 predicted fragment length is 123 bps INFO @ Sat, 11 Dec 2021 06:29:54: #2 alternative fragment length(s) may be 123 bps INFO @ Sat, 11 Dec 2021 06:29:54: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.10_model.r WARNING @ Sat, 11 Dec 2021 06:29:54: #2 Since the d (123) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:29:54: #2 You may need to consider one of the other alternative d(s): 123 WARNING @ Sat, 11 Dec 2021 06:29:54: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:29:54: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:29:54: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:29:59: 66000000 INFO @ Sat, 11 Dec 2021 06:30:00: #1 tag size is determined as 116 bps INFO @ Sat, 11 Dec 2021 06:30:00: #1 tag size = 116 INFO @ Sat, 11 Dec 2021 06:30:00: #1 total tags in treatment: 27067834 INFO @ Sat, 11 Dec 2021 06:30:00: #1 user defined the maximum tags... INFO @ Sat, 11 Dec 2021 06:30:00: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sat, 11 Dec 2021 06:30:01: #1 tags after filtering in treatment: 20270983 INFO @ Sat, 11 Dec 2021 06:30:01: #1 Redundant rate of treatment: 0.25 INFO @ Sat, 11 Dec 2021 06:30:01: #1 finished! INFO @ Sat, 11 Dec 2021 06:30:01: #2 Build Peak Model... INFO @ Sat, 11 Dec 2021 06:30:01: #2 looking for paired plus/minus strand peaks... INFO @ Sat, 11 Dec 2021 06:30:02: #2 number of paired peaks: 963 WARNING @ Sat, 11 Dec 2021 06:30:02: Fewer paired peaks (963) than 1000! Model may not be build well! Lower your MFOLD parameter may erase this warning. Now I will use 963 pairs to build model! INFO @ Sat, 11 Dec 2021 06:30:02: start model_add_line... INFO @ Sat, 11 Dec 2021 06:30:02: start X-correlation... INFO @ Sat, 11 Dec 2021 06:30:02: end of X-cor INFO @ Sat, 11 Dec 2021 06:30:02: #2 finished! INFO @ Sat, 11 Dec 2021 06:30:02: #2 predicted fragment length is 123 bps INFO @ Sat, 11 Dec 2021 06:30:02: #2 alternative fragment length(s) may be 123 bps INFO @ Sat, 11 Dec 2021 06:30:02: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.20_model.r WARNING @ Sat, 11 Dec 2021 06:30:02: #2 Since the d (123) calculated from paired-peaks are smaller than 2*tag length, it may be influenced by unknown sequencing problem! WARNING @ Sat, 11 Dec 2021 06:30:02: #2 You may need to consider one of the other alternative d(s): 123 WARNING @ Sat, 11 Dec 2021 06:30:02: #2 You can restart the process with --nomodel --extsize XXX with your choice or an arbitrary number. Nontheless, MACS will continute computing. INFO @ Sat, 11 Dec 2021 06:30:02: #3 Call peaks... INFO @ Sat, 11 Dec 2021 06:30:02: #3 Pre-compute pvalue-qvalue table... INFO @ Sat, 11 Dec 2021 06:30:33: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:30:40: #3 Call peaks for each chromosome... INFO @ Sat, 11 Dec 2021 06:30:53: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.10_peaks.xls INFO @ Sat, 11 Dec 2021 06:30:53: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.10_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:30:54: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.10_summits.bed INFO @ Sat, 11 Dec 2021 06:30:54: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (8943 records, 4 fields): 10 millis CompletedMACS2peakCalling INFO @ Sat, 11 Dec 2021 06:31:00: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.20_peaks.xls INFO @ Sat, 11 Dec 2021 06:31:00: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.20_peaks.narrowPeak INFO @ Sat, 11 Dec 2021 06:31:00: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX3978793/ERX3978793.20_summits.bed INFO @ Sat, 11 Dec 2021 06:31:00: Done! pass1 - making usageList (14 chroms): 1 millis pass2 - checking and writing primary data (4966 records, 4 fields): 6 millis CompletedMACS2peakCalling