Job ID = 1293428 sra ファイルのダウンロード中... Read layout: SINGLE fastq に変換中... spots read : 3,220,885 reads read : 3,220,885 reads written : 3,220,885 rm: cannot remove ‘[DSE]RR*’: No such file or directory rm: cannot remove ‘fastqDump_tmp*’: No such file or directory fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 00:01:01 3220885 reads; of these: 3220885 (100.00%) were unpaired; of these: 106995 (3.32%) aligned 0 times 2350216 (72.97%) aligned exactly 1 time 763674 (23.71%) aligned >1 times 96.68% overall alignment rate Time searching: 00:01:01 Overall time: 00:01:01 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_rmdupse_core] 185969 / 3113890 = 0.0597 in library ' ' BAM に変換しました。 Bed ファイルを作成中... BedGraph に変換中... INFO @ Sun, 02 Jun 2019 23:47:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:47:47: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:47:47: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:47:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:47:47: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:47:47: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:47:47: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Sun, 02 Jun 2019 23:47:47: #1 read tag files... INFO @ Sun, 02 Jun 2019 23:47:47: #1 read treatment tags... INFO @ Sun, 02 Jun 2019 23:47:55: 1000000 INFO @ Sun, 02 Jun 2019 23:47:56: 1000000 INFO @ Sun, 02 Jun 2019 23:47:56: 1000000 INFO @ Sun, 02 Jun 2019 23:48:03: 2000000 INFO @ Sun, 02 Jun 2019 23:48:04: 2000000 INFO @ Sun, 02 Jun 2019 23:48:05: 2000000 INFO @ Sun, 02 Jun 2019 23:48:10: #1 tag size is determined as 30 bps INFO @ Sun, 02 Jun 2019 23:48:10: #1 tag size = 30 INFO @ Sun, 02 Jun 2019 23:48:10: #1 total tags in treatment: 2927921 INFO @ Sun, 02 Jun 2019 23:48:10: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:48:10: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:48:10: #1 tags after filtering in treatment: 2927921 INFO @ Sun, 02 Jun 2019 23:48:10: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:48:10: #1 finished! INFO @ Sun, 02 Jun 2019 23:48:10: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:48:10: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:48:11: #2 number of paired peaks: 2608 INFO @ Sun, 02 Jun 2019 23:48:11: start model_add_line... INFO @ Sun, 02 Jun 2019 23:48:11: start X-correlation... INFO @ Sun, 02 Jun 2019 23:48:11: end of X-cor INFO @ Sun, 02 Jun 2019 23:48:11: #2 finished! INFO @ Sun, 02 Jun 2019 23:48:11: #2 predicted fragment length is 119 bps INFO @ Sun, 02 Jun 2019 23:48:11: #2 alternative fragment length(s) may be 119 bps INFO @ Sun, 02 Jun 2019 23:48:11: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.10_model.r INFO @ Sun, 02 Jun 2019 23:48:11: #3 Call peaks... INFO @ Sun, 02 Jun 2019 23:48:11: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 23:48:12: #1 tag size is determined as 30 bps INFO @ Sun, 02 Jun 2019 23:48:12: #1 tag size = 30 INFO @ Sun, 02 Jun 2019 23:48:12: #1 total tags in treatment: 2927921 INFO @ Sun, 02 Jun 2019 23:48:12: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:48:12: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:48:12: #1 tags after filtering in treatment: 2927921 INFO @ Sun, 02 Jun 2019 23:48:12: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:48:12: #1 finished! INFO @ Sun, 02 Jun 2019 23:48:12: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:48:12: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:48:12: #2 number of paired peaks: 2608 INFO @ Sun, 02 Jun 2019 23:48:12: start model_add_line... INFO @ Sun, 02 Jun 2019 23:48:12: start X-correlation... INFO @ Sun, 02 Jun 2019 23:48:12: end of X-cor INFO @ Sun, 02 Jun 2019 23:48:12: #2 finished! INFO @ Sun, 02 Jun 2019 23:48:12: #2 predicted fragment length is 119 bps INFO @ Sun, 02 Jun 2019 23:48:12: #2 alternative fragment length(s) may be 119 bps INFO @ Sun, 02 Jun 2019 23:48:12: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.20_model.r INFO @ Sun, 02 Jun 2019 23:48:12: #3 Call peaks... INFO @ Sun, 02 Jun 2019 23:48:12: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 23:48:14: #1 tag size is determined as 30 bps INFO @ Sun, 02 Jun 2019 23:48:14: #1 tag size = 30 INFO @ Sun, 02 Jun 2019 23:48:14: #1 total tags in treatment: 2927921 INFO @ Sun, 02 Jun 2019 23:48:14: #1 user defined the maximum tags... INFO @ Sun, 02 Jun 2019 23:48:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Sun, 02 Jun 2019 23:48:14: #1 tags after filtering in treatment: 2927921 INFO @ Sun, 02 Jun 2019 23:48:14: #1 Redundant rate of treatment: 0.00 INFO @ Sun, 02 Jun 2019 23:48:14: #1 finished! INFO @ Sun, 02 Jun 2019 23:48:14: #2 Build Peak Model... INFO @ Sun, 02 Jun 2019 23:48:14: #2 looking for paired plus/minus strand peaks... INFO @ Sun, 02 Jun 2019 23:48:14: #2 number of paired peaks: 2608 INFO @ Sun, 02 Jun 2019 23:48:14: start model_add_line... INFO @ Sun, 02 Jun 2019 23:48:14: start X-correlation... INFO @ Sun, 02 Jun 2019 23:48:14: end of X-cor INFO @ Sun, 02 Jun 2019 23:48:14: #2 finished! INFO @ Sun, 02 Jun 2019 23:48:14: #2 predicted fragment length is 119 bps INFO @ Sun, 02 Jun 2019 23:48:14: #2 alternative fragment length(s) may be 119 bps INFO @ Sun, 02 Jun 2019 23:48:14: #2.2 Generate R script for model : /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.05_model.r INFO @ Sun, 02 Jun 2019 23:48:14: #3 Call peaks... INFO @ Sun, 02 Jun 2019 23:48:14: #3 Pre-compute pvalue-qvalue table... INFO @ Sun, 02 Jun 2019 23:48:20: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 23:48:21: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 23:48:24: #3 Call peaks for each chromosome... INFO @ Sun, 02 Jun 2019 23:48:25: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.10_peaks.xls INFO @ Sun, 02 Jun 2019 23:48:25: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.10_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 23:48:25: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.10_summits.bed INFO @ Sun, 02 Jun 2019 23:48:25: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (2884 records, 4 fields): 6 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:48:26: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.20_peaks.xls INFO @ Sun, 02 Jun 2019 23:48:26: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.20_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 23:48:26: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.20_summits.bed INFO @ Sun, 02 Jun 2019 23:48:26: Done! pass1 - making usageList (15 chroms): 1 millis pass2 - checking and writing primary data (1082 records, 4 fields): 5 millis CompletedMACS2peakCalling INFO @ Sun, 02 Jun 2019 23:48:29: #4 Write output xls file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.05_peaks.xls INFO @ Sun, 02 Jun 2019 23:48:29: #4 Write peak in narrowPeak format file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.05_peaks.narrowPeak INFO @ Sun, 02 Jun 2019 23:48:29: #4 Write summits bed file... /home/okishinya/chipatlas/results/dm3/ERX242713/ERX242713.05_summits.bed INFO @ Sun, 02 Jun 2019 23:48:29: Done! pass1 - making usageList (15 chroms): 2 millis pass2 - checking and writing primary data (6049 records, 4 fields): 9 millis CompletedMACS2peakCalling BedGraph に変換しました。 BigWig に変換中... BigWig に変換しました。