Job ID = 6527433 SRX = ERX1818280 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-29T13:42:45 prefetch.2.10.7: 1) Downloading 'ERR1749083'... 2020-06-29T13:42:45 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:49:53 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:49:53 prefetch.2.10.7: 1) 'ERR1749083' was downloaded successfully Read 26942224 spots for ERR1749083/ERR1749083.sra Written 26942224 spots for ERR1749083/ERR1749083.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:25:53 26942224 reads; of these: 26942224 (100.00%) were paired; of these: 2191494 (8.13%) aligned concordantly 0 times 17951041 (66.63%) aligned concordantly exactly 1 time 6799689 (25.24%) aligned concordantly >1 times ---- 2191494 pairs aligned concordantly 0 times; of these: 381655 (17.42%) aligned discordantly 1 time ---- 1809839 pairs aligned 0 times concordantly or discordantly; of these: 3619678 mates make up the pairs; of these: 2011281 (55.57%) aligned 0 times 837514 (23.14%) aligned exactly 1 time 770883 (21.30%) aligned >1 times 96.27% overall alignment rate Time searching: 01:25:53 Overall time: 01:25:53 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 1319043 / 24897240 = 0.0530 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:02:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:02:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:02:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:02:06: 1000000 INFO @ Tue, 30 Jun 2020 01:02:11: 2000000 INFO @ Tue, 30 Jun 2020 01:02:17: 3000000 INFO @ Tue, 30 Jun 2020 01:02:22: 4000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:02:28: 5000000 INFO @ Tue, 30 Jun 2020 01:02:30: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:02:30: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:02:30: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:02:34: 6000000 INFO @ Tue, 30 Jun 2020 01:02:35: 1000000 INFO @ Tue, 30 Jun 2020 01:02:40: 7000000 INFO @ Tue, 30 Jun 2020 01:02:41: 2000000 INFO @ Tue, 30 Jun 2020 01:02:45: 8000000 INFO @ Tue, 30 Jun 2020 01:02:46: 3000000 INFO @ Tue, 30 Jun 2020 01:02:51: 9000000 INFO @ Tue, 30 Jun 2020 01:02:51: 4000000 INFO @ Tue, 30 Jun 2020 01:02:57: 5000000 INFO @ Tue, 30 Jun 2020 01:02:57: 10000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 01:03:00: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 01:03:00: #1 read tag files... INFO @ Tue, 30 Jun 2020 01:03:00: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 01:03:03: 6000000 INFO @ Tue, 30 Jun 2020 01:03:03: 11000000 INFO @ Tue, 30 Jun 2020 01:03:07: 1000000 INFO @ Tue, 30 Jun 2020 01:03:09: 7000000 INFO @ Tue, 30 Jun 2020 01:03:09: 12000000 INFO @ Tue, 30 Jun 2020 01:03:14: 2000000 INFO @ Tue, 30 Jun 2020 01:03:15: 13000000 INFO @ Tue, 30 Jun 2020 01:03:15: 8000000 INFO @ Tue, 30 Jun 2020 01:03:20: 9000000 INFO @ Tue, 30 Jun 2020 01:03:21: 14000000 INFO @ Tue, 30 Jun 2020 01:03:21: 3000000 INFO @ Tue, 30 Jun 2020 01:03:27: 15000000 INFO @ Tue, 30 Jun 2020 01:03:27: 10000000 INFO @ Tue, 30 Jun 2020 01:03:28: 4000000 INFO @ Tue, 30 Jun 2020 01:03:32: 16000000 INFO @ Tue, 30 Jun 2020 01:03:33: 11000000 INFO @ Tue, 30 Jun 2020 01:03:34: 5000000 INFO @ Tue, 30 Jun 2020 01:03:38: 17000000 INFO @ Tue, 30 Jun 2020 01:03:39: 12000000 INFO @ Tue, 30 Jun 2020 01:03:41: 6000000 INFO @ Tue, 30 Jun 2020 01:03:44: 18000000 INFO @ Tue, 30 Jun 2020 01:03:45: 13000000 INFO @ Tue, 30 Jun 2020 01:03:47: 7000000 INFO @ Tue, 30 Jun 2020 01:03:50: 19000000 INFO @ Tue, 30 Jun 2020 01:03:51: 14000000 INFO @ Tue, 30 Jun 2020 01:03:54: 8000000 INFO @ Tue, 30 Jun 2020 01:03:56: 20000000 INFO @ Tue, 30 Jun 2020 01:03:58: 15000000 INFO @ Tue, 30 Jun 2020 01:04:00: 9000000 INFO @ Tue, 30 Jun 2020 01:04:02: 21000000 INFO @ Tue, 30 Jun 2020 01:04:04: 16000000 INFO @ Tue, 30 Jun 2020 01:04:07: 10000000 INFO @ Tue, 30 Jun 2020 01:04:07: 22000000 INFO @ Tue, 30 Jun 2020 01:04:11: 17000000 INFO @ Tue, 30 Jun 2020 01:04:13: 23000000 INFO @ Tue, 30 Jun 2020 01:04:13: 11000000 INFO @ Tue, 30 Jun 2020 01:04:17: 18000000 INFO @ Tue, 30 Jun 2020 01:04:19: 24000000 INFO @ Tue, 30 Jun 2020 01:04:20: 12000000 INFO @ Tue, 30 Jun 2020 01:04:24: 19000000 INFO @ Tue, 30 Jun 2020 01:04:25: 25000000 INFO @ Tue, 30 Jun 2020 01:04:26: 13000000 INFO @ Tue, 30 Jun 2020 01:04:30: 20000000 INFO @ Tue, 30 Jun 2020 01:04:31: 26000000 INFO @ Tue, 30 Jun 2020 01:04:33: 14000000 INFO @ Tue, 30 Jun 2020 01:04:36: 27000000 INFO @ Tue, 30 Jun 2020 01:04:37: 21000000 INFO @ Tue, 30 Jun 2020 01:04:40: 15000000 INFO @ Tue, 30 Jun 2020 01:04:42: 28000000 INFO @ Tue, 30 Jun 2020 01:04:43: 22000000 INFO @ Tue, 30 Jun 2020 01:04:47: 16000000 INFO @ Tue, 30 Jun 2020 01:04:48: 29000000 INFO @ Tue, 30 Jun 2020 01:04:49: 23000000 INFO @ Tue, 30 Jun 2020 01:04:53: 17000000 INFO @ Tue, 30 Jun 2020 01:04:54: 30000000 INFO @ Tue, 30 Jun 2020 01:04:56: 24000000 INFO @ Tue, 30 Jun 2020 01:05:00: 18000000 INFO @ Tue, 30 Jun 2020 01:05:00: 31000000 INFO @ Tue, 30 Jun 2020 01:05:02: 25000000 INFO @ Tue, 30 Jun 2020 01:05:06: 19000000 INFO @ Tue, 30 Jun 2020 01:05:06: 32000000 INFO @ Tue, 30 Jun 2020 01:05:08: 26000000 INFO @ Tue, 30 Jun 2020 01:05:12: 20000000 INFO @ Tue, 30 Jun 2020 01:05:12: 33000000 INFO @ Tue, 30 Jun 2020 01:05:14: 27000000 INFO @ Tue, 30 Jun 2020 01:05:19: 34000000 INFO @ Tue, 30 Jun 2020 01:05:19: 21000000 INFO @ Tue, 30 Jun 2020 01:05:21: 28000000 INFO @ Tue, 30 Jun 2020 01:05:25: 35000000 INFO @ Tue, 30 Jun 2020 01:05:25: 22000000 INFO @ Tue, 30 Jun 2020 01:05:28: 29000000 INFO @ Tue, 30 Jun 2020 01:05:31: 36000000 INFO @ Tue, 30 Jun 2020 01:05:32: 23000000 INFO @ Tue, 30 Jun 2020 01:05:34: 30000000 INFO @ Tue, 30 Jun 2020 01:05:37: 37000000 INFO @ Tue, 30 Jun 2020 01:05:38: 24000000 INFO @ Tue, 30 Jun 2020 01:05:41: 31000000 INFO @ Tue, 30 Jun 2020 01:05:43: 38000000 INFO @ Tue, 30 Jun 2020 01:05:45: 25000000 INFO @ Tue, 30 Jun 2020 01:05:47: 32000000 INFO @ Tue, 30 Jun 2020 01:05:49: 39000000 INFO @ Tue, 30 Jun 2020 01:05:52: 26000000 INFO @ Tue, 30 Jun 2020 01:05:54: 33000000 INFO @ Tue, 30 Jun 2020 01:05:55: 40000000 INFO @ Tue, 30 Jun 2020 01:05:58: 27000000 INFO @ Tue, 30 Jun 2020 01:06:01: 34000000 INFO @ Tue, 30 Jun 2020 01:06:02: 41000000 INFO @ Tue, 30 Jun 2020 01:06:05: 28000000 INFO @ Tue, 30 Jun 2020 01:06:07: 35000000 INFO @ Tue, 30 Jun 2020 01:06:08: 42000000 INFO @ Tue, 30 Jun 2020 01:06:12: 29000000 INFO @ Tue, 30 Jun 2020 01:06:14: 36000000 INFO @ Tue, 30 Jun 2020 01:06:14: 43000000 INFO @ Tue, 30 Jun 2020 01:06:18: 30000000 INFO @ Tue, 30 Jun 2020 01:06:20: 44000000 INFO @ Tue, 30 Jun 2020 01:06:21: 37000000 INFO @ Tue, 30 Jun 2020 01:06:25: 31000000 INFO @ Tue, 30 Jun 2020 01:06:26: 45000000 INFO @ Tue, 30 Jun 2020 01:06:27: 38000000 INFO @ Tue, 30 Jun 2020 01:06:32: 32000000 INFO @ Tue, 30 Jun 2020 01:06:33: 46000000 INFO @ Tue, 30 Jun 2020 01:06:34: 39000000 INFO @ Tue, 30 Jun 2020 01:06:38: 33000000 INFO @ Tue, 30 Jun 2020 01:06:39: 47000000 INFO @ Tue, 30 Jun 2020 01:06:41: 40000000 INFO @ Tue, 30 Jun 2020 01:06:45: 34000000 INFO @ Tue, 30 Jun 2020 01:06:45: 48000000 INFO @ Tue, 30 Jun 2020 01:06:47: 41000000 INFO @ Tue, 30 Jun 2020 01:06:51: 49000000 INFO @ Tue, 30 Jun 2020 01:06:51: 35000000 INFO @ Tue, 30 Jun 2020 01:06:53: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:06:53: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:06:53: #1 total tags in treatment: 23436294 INFO @ Tue, 30 Jun 2020 01:06:53: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:06:53: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:06:53: #1 tags after filtering in treatment: 21789441 INFO @ Tue, 30 Jun 2020 01:06:53: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 30 Jun 2020 01:06:53: #1 finished! INFO @ Tue, 30 Jun 2020 01:06:53: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:06:53: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:06:54: 42000000 INFO @ Tue, 30 Jun 2020 01:06:55: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 01:06:55: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:06:55: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:06:58: 36000000 INFO @ Tue, 30 Jun 2020 01:07:01: 43000000 INFO @ Tue, 30 Jun 2020 01:07:04: 37000000 INFO @ Tue, 30 Jun 2020 01:07:07: 44000000 INFO @ Tue, 30 Jun 2020 01:07:11: 38000000 INFO @ Tue, 30 Jun 2020 01:07:14: 45000000 INFO @ Tue, 30 Jun 2020 01:07:17: 39000000 INFO @ Tue, 30 Jun 2020 01:07:20: 46000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 01:07:24: 40000000 INFO @ Tue, 30 Jun 2020 01:07:27: 47000000 INFO @ Tue, 30 Jun 2020 01:07:30: 41000000 INFO @ Tue, 30 Jun 2020 01:07:33: 48000000 INFO @ Tue, 30 Jun 2020 01:07:37: 42000000 INFO @ Tue, 30 Jun 2020 01:07:40: 49000000 INFO @ Tue, 30 Jun 2020 01:07:41: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:07:41: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:07:41: #1 total tags in treatment: 23436294 INFO @ Tue, 30 Jun 2020 01:07:41: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:07:41: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:07:42: #1 tags after filtering in treatment: 21789441 INFO @ Tue, 30 Jun 2020 01:07:42: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 30 Jun 2020 01:07:42: #1 finished! INFO @ Tue, 30 Jun 2020 01:07:42: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:07:42: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:07:43: 43000000 INFO @ Tue, 30 Jun 2020 01:07:43: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 01:07:43: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:07:43: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 01:07:49: 44000000 INFO @ Tue, 30 Jun 2020 01:07:55: 45000000 INFO @ Tue, 30 Jun 2020 01:08:00: 46000000 INFO @ Tue, 30 Jun 2020 01:08:06: 47000000 INFO @ Tue, 30 Jun 2020 01:08:12: 48000000 INFO @ Tue, 30 Jun 2020 01:08:17: 49000000 INFO @ Tue, 30 Jun 2020 01:08:19: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 01:08:19: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 01:08:19: #1 total tags in treatment: 23436294 INFO @ Tue, 30 Jun 2020 01:08:19: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 01:08:19: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 01:08:19: #1 tags after filtering in treatment: 21789441 INFO @ Tue, 30 Jun 2020 01:08:19: #1 Redundant rate of treatment: 0.07 INFO @ Tue, 30 Jun 2020 01:08:19: #1 finished! INFO @ Tue, 30 Jun 2020 01:08:19: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 01:08:19: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 01:08:21: #2 number of paired peaks: 67 WARNING @ Tue, 30 Jun 2020 01:08:21: Too few paired peaks (67) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 01:08:21: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818280/ERX1818280.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling BigWig に変換しました。