Job ID = 6527429 SRX = ERX1818268 Genome = dm3 sra ファイルのダウンロード中... Read layout: PAIRED fastq に変換中... 2020-06-29T13:05:54 prefetch.2.10.7: 1) Downloading 'ERR1749071'... 2020-06-29T13:05:54 prefetch.2.10.7: Downloading via HTTPS... 2020-06-29T13:13:18 prefetch.2.10.7: HTTPS download succeed 2020-06-29T13:13:18 prefetch.2.10.7: 1) 'ERR1749071' was downloaded successfully Read 30282258 spots for ERR1749071/ERR1749071.sra Written 30282258 spots for ERR1749071/ERR1749071.sra fastq に変換しました。 bowtie でマッピング中... Time loading reference: 00:00:00 Time loading forward index: 00:00:00 Time loading mirror index: 00:00:00 Multiseed full-index search: 01:33:50 30282258 reads; of these: 30282258 (100.00%) were paired; of these: 2135851 (7.05%) aligned concordantly 0 times 16003560 (52.85%) aligned concordantly exactly 1 time 12142847 (40.10%) aligned concordantly >1 times ---- 2135851 pairs aligned concordantly 0 times; of these: 309847 (14.51%) aligned discordantly 1 time ---- 1826004 pairs aligned 0 times concordantly or discordantly; of these: 3652008 mates make up the pairs; of these: 2286410 (62.61%) aligned 0 times 525487 (14.39%) aligned exactly 1 time 840111 (23.00%) aligned >1 times 96.22% overall alignment rate Time searching: 01:33:50 Overall time: 01:33:50 マッピングが完了しました。 samtools でBAM に変換中... [samopen] SAM header is present: 15 sequences. [bam_sort_core] merging from 24 files... [bam_rmdup_core] processing reference chr2L... [bam_rmdup_core] processing reference chr2LHet... [bam_rmdup_core] processing reference chr2R... [bam_rmdup_core] processing reference chr2RHet... [bam_rmdup_core] processing reference chr3L... [bam_rmdup_core] processing reference chr3LHet... [bam_rmdup_core] processing reference chr3R... [bam_rmdup_core] processing reference chr3RHet... [bam_rmdup_core] processing reference chr4... [bam_rmdup_core] processing reference chrM... [bam_rmdup_core] processing reference chrU... [bam_rmdup_core] processing reference chrUextra... [bam_rmdup_core] processing reference chrX... [bam_rmdup_core] processing reference chrXHet... [bam_rmdup_core] processing reference chrYHet... [bam_rmdup_core] 3628037 / 28366653 = 0.1279 in library ' ' BAM に変換しました。 Bed ファイルを作成中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:38:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.05 -q 1e-05 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.05 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-05 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:38:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:38:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:38:23: 1000000 INFO @ Tue, 30 Jun 2020 00:38:30: 2000000 INFO @ Tue, 30 Jun 2020 00:38:38: 3000000 WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:38:45: 4000000 INFO @ Tue, 30 Jun 2020 00:38:46: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.10 -q 1e-10 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.10 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-10 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:38:46: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:38:46: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:38:53: 5000000 INFO @ Tue, 30 Jun 2020 00:38:54: 1000000 INFO @ Tue, 30 Jun 2020 00:39:01: 6000000 INFO @ Tue, 30 Jun 2020 00:39:02: 2000000 INFO @ Tue, 30 Jun 2020 00:39:09: 7000000 INFO @ Tue, 30 Jun 2020 00:39:11: 3000000 BedGraph に変換中... WARNING: Skipping mount /opt/pkg/singularity/3.5.2/var/singularity/mnt/session/etc/resolv.conf [files]: /etc/resolv.conf doesn't exist in container INFO @ Tue, 30 Jun 2020 00:39:16: # Command line: callpeak -t /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.bam -f BAM -g dm -n /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.20 -q 1e-20 # ARGUMENTS LIST: # name = /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.20 # format = BAM # ChIP-seq file = ['/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.bam'] # control file = None # effective genome size = 1.20e+08 # band width = 300 # model fold = [5, 50] # qvalue cutoff = 1.00e-20 # Larger dataset will be scaled towards smaller dataset. # Range for calculating regional lambda is: 10000 bps # Broad region calling is off # Paired-End mode is off INFO @ Tue, 30 Jun 2020 00:39:16: #1 read tag files... INFO @ Tue, 30 Jun 2020 00:39:16: #1 read treatment tags... INFO @ Tue, 30 Jun 2020 00:39:18: 8000000 INFO @ Tue, 30 Jun 2020 00:39:19: 4000000 INFO @ Tue, 30 Jun 2020 00:39:25: 1000000 INFO @ Tue, 30 Jun 2020 00:39:27: 9000000 INFO @ Tue, 30 Jun 2020 00:39:28: 5000000 INFO @ Tue, 30 Jun 2020 00:39:34: 2000000 INFO @ Tue, 30 Jun 2020 00:39:36: 10000000 INFO @ Tue, 30 Jun 2020 00:39:37: 6000000 INFO @ Tue, 30 Jun 2020 00:39:43: 3000000 INFO @ Tue, 30 Jun 2020 00:39:45: 11000000 INFO @ Tue, 30 Jun 2020 00:39:46: 7000000 INFO @ Tue, 30 Jun 2020 00:39:52: 4000000 INFO @ Tue, 30 Jun 2020 00:39:54: 12000000 INFO @ Tue, 30 Jun 2020 00:39:55: 8000000 INFO @ Tue, 30 Jun 2020 00:40:01: 5000000 INFO @ Tue, 30 Jun 2020 00:40:02: 13000000 INFO @ Tue, 30 Jun 2020 00:40:04: 9000000 INFO @ Tue, 30 Jun 2020 00:40:10: 6000000 INFO @ Tue, 30 Jun 2020 00:40:12: 14000000 INFO @ Tue, 30 Jun 2020 00:40:13: 10000000 INFO @ Tue, 30 Jun 2020 00:40:19: 7000000 INFO @ Tue, 30 Jun 2020 00:40:21: 15000000 INFO @ Tue, 30 Jun 2020 00:40:22: 11000000 INFO @ Tue, 30 Jun 2020 00:40:28: 8000000 INFO @ Tue, 30 Jun 2020 00:40:30: 16000000 INFO @ Tue, 30 Jun 2020 00:40:31: 12000000 INFO @ Tue, 30 Jun 2020 00:40:37: 9000000 INFO @ Tue, 30 Jun 2020 00:40:39: 17000000 INFO @ Tue, 30 Jun 2020 00:40:40: 13000000 INFO @ Tue, 30 Jun 2020 00:40:46: 10000000 INFO @ Tue, 30 Jun 2020 00:40:48: 18000000 INFO @ Tue, 30 Jun 2020 00:40:49: 14000000 INFO @ Tue, 30 Jun 2020 00:40:55: 11000000 INFO @ Tue, 30 Jun 2020 00:40:57: 19000000 INFO @ Tue, 30 Jun 2020 00:40:58: 15000000 INFO @ Tue, 30 Jun 2020 00:41:04: 12000000 INFO @ Tue, 30 Jun 2020 00:41:06: 20000000 INFO @ Tue, 30 Jun 2020 00:41:07: 16000000 INFO @ Tue, 30 Jun 2020 00:41:13: 13000000 INFO @ Tue, 30 Jun 2020 00:41:15: 21000000 INFO @ Tue, 30 Jun 2020 00:41:15: 17000000 INFO @ Tue, 30 Jun 2020 00:41:22: 14000000 INFO @ Tue, 30 Jun 2020 00:41:24: 22000000 INFO @ Tue, 30 Jun 2020 00:41:24: 18000000 INFO @ Tue, 30 Jun 2020 00:41:31: 15000000 INFO @ Tue, 30 Jun 2020 00:41:32: 23000000 INFO @ Tue, 30 Jun 2020 00:41:33: 19000000 INFO @ Tue, 30 Jun 2020 00:41:40: 16000000 INFO @ Tue, 30 Jun 2020 00:41:41: 24000000 INFO @ Tue, 30 Jun 2020 00:41:42: 20000000 INFO @ Tue, 30 Jun 2020 00:41:49: 17000000 INFO @ Tue, 30 Jun 2020 00:41:50: 25000000 INFO @ Tue, 30 Jun 2020 00:41:51: 21000000 INFO @ Tue, 30 Jun 2020 00:41:57: 18000000 INFO @ Tue, 30 Jun 2020 00:41:59: 26000000 INFO @ Tue, 30 Jun 2020 00:42:00: 22000000 INFO @ Tue, 30 Jun 2020 00:42:06: 19000000 INFO @ Tue, 30 Jun 2020 00:42:08: 27000000 INFO @ Tue, 30 Jun 2020 00:42:09: 23000000 INFO @ Tue, 30 Jun 2020 00:42:15: 20000000 INFO @ Tue, 30 Jun 2020 00:42:17: 28000000 INFO @ Tue, 30 Jun 2020 00:42:18: 24000000 INFO @ Tue, 30 Jun 2020 00:42:24: 21000000 INFO @ Tue, 30 Jun 2020 00:42:26: 29000000 INFO @ Tue, 30 Jun 2020 00:42:27: 25000000 INFO @ Tue, 30 Jun 2020 00:42:32: 22000000 INFO @ Tue, 30 Jun 2020 00:42:35: 30000000 INFO @ Tue, 30 Jun 2020 00:42:36: 26000000 INFO @ Tue, 30 Jun 2020 00:42:41: 23000000 INFO @ Tue, 30 Jun 2020 00:42:44: 31000000 INFO @ Tue, 30 Jun 2020 00:42:44: 27000000 INFO @ Tue, 30 Jun 2020 00:42:50: 24000000 INFO @ Tue, 30 Jun 2020 00:42:52: 32000000 INFO @ Tue, 30 Jun 2020 00:42:53: 28000000 INFO @ Tue, 30 Jun 2020 00:42:59: 25000000 INFO @ Tue, 30 Jun 2020 00:43:01: 33000000 INFO @ Tue, 30 Jun 2020 00:43:02: 29000000 INFO @ Tue, 30 Jun 2020 00:43:08: 26000000 INFO @ Tue, 30 Jun 2020 00:43:09: 34000000 INFO @ Tue, 30 Jun 2020 00:43:11: 30000000 INFO @ Tue, 30 Jun 2020 00:43:17: 27000000 INFO @ Tue, 30 Jun 2020 00:43:18: 35000000 INFO @ Tue, 30 Jun 2020 00:43:20: 31000000 INFO @ Tue, 30 Jun 2020 00:43:25: 28000000 INFO @ Tue, 30 Jun 2020 00:43:27: 36000000 INFO @ Tue, 30 Jun 2020 00:43:29: 32000000 INFO @ Tue, 30 Jun 2020 00:43:34: 29000000 INFO @ Tue, 30 Jun 2020 00:43:35: 37000000 INFO @ Tue, 30 Jun 2020 00:43:37: 33000000 BedGraph に変換しました。 BigWig に変換中... INFO @ Tue, 30 Jun 2020 00:43:43: 30000000 INFO @ Tue, 30 Jun 2020 00:43:43: 38000000 INFO @ Tue, 30 Jun 2020 00:43:46: 34000000 INFO @ Tue, 30 Jun 2020 00:43:52: 31000000 INFO @ Tue, 30 Jun 2020 00:43:52: 39000000 INFO @ Tue, 30 Jun 2020 00:43:54: 35000000 INFO @ Tue, 30 Jun 2020 00:44:00: 32000000 INFO @ Tue, 30 Jun 2020 00:44:01: 40000000 INFO @ Tue, 30 Jun 2020 00:44:03: 36000000 INFO @ Tue, 30 Jun 2020 00:44:08: 33000000 INFO @ Tue, 30 Jun 2020 00:44:10: 41000000 INFO @ Tue, 30 Jun 2020 00:44:12: 37000000 INFO @ Tue, 30 Jun 2020 00:44:17: 34000000 INFO @ Tue, 30 Jun 2020 00:44:18: 42000000 INFO @ Tue, 30 Jun 2020 00:44:20: 38000000 INFO @ Tue, 30 Jun 2020 00:44:26: 35000000 INFO @ Tue, 30 Jun 2020 00:44:27: 43000000 INFO @ Tue, 30 Jun 2020 00:44:29: 39000000 INFO @ Tue, 30 Jun 2020 00:44:34: 36000000 INFO @ Tue, 30 Jun 2020 00:44:36: 44000000 INFO @ Tue, 30 Jun 2020 00:44:38: 40000000 INFO @ Tue, 30 Jun 2020 00:44:43: 37000000 INFO @ Tue, 30 Jun 2020 00:44:45: 45000000 INFO @ Tue, 30 Jun 2020 00:44:46: 41000000 INFO @ Tue, 30 Jun 2020 00:44:51: 38000000 INFO @ Tue, 30 Jun 2020 00:44:54: 46000000 INFO @ Tue, 30 Jun 2020 00:44:55: 42000000 INFO @ Tue, 30 Jun 2020 00:44:59: 39000000 INFO @ Tue, 30 Jun 2020 00:45:03: 47000000 INFO @ Tue, 30 Jun 2020 00:45:04: 43000000 INFO @ Tue, 30 Jun 2020 00:45:08: 40000000 INFO @ Tue, 30 Jun 2020 00:45:12: 48000000 INFO @ Tue, 30 Jun 2020 00:45:13: 44000000 INFO @ Tue, 30 Jun 2020 00:45:16: 41000000 INFO @ Tue, 30 Jun 2020 00:45:21: 49000000 INFO @ Tue, 30 Jun 2020 00:45:22: 45000000 INFO @ Tue, 30 Jun 2020 00:45:25: 42000000 BigWig に変換しました。 INFO @ Tue, 30 Jun 2020 00:45:30: 50000000 INFO @ Tue, 30 Jun 2020 00:45:31: 46000000 INFO @ Tue, 30 Jun 2020 00:45:33: 43000000 INFO @ Tue, 30 Jun 2020 00:45:38: 51000000 INFO @ Tue, 30 Jun 2020 00:45:39: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:45:39: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:45:39: #1 total tags in treatment: 24523233 INFO @ Tue, 30 Jun 2020 00:45:39: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:45:39: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:45:39: #1 tags after filtering in treatment: 20358590 INFO @ Tue, 30 Jun 2020 00:45:39: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 30 Jun 2020 00:45:39: #1 finished! INFO @ Tue, 30 Jun 2020 00:45:39: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:45:39: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:45:40: 47000000 INFO @ Tue, 30 Jun 2020 00:45:41: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 00:45:41: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:45:41: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.05_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.05_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.05_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.05_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:45:42: 44000000 INFO @ Tue, 30 Jun 2020 00:45:48: 48000000 INFO @ Tue, 30 Jun 2020 00:45:51: 45000000 INFO @ Tue, 30 Jun 2020 00:45:57: 49000000 INFO @ Tue, 30 Jun 2020 00:46:00: 46000000 INFO @ Tue, 30 Jun 2020 00:46:05: 50000000 INFO @ Tue, 30 Jun 2020 00:46:08: 47000000 INFO @ Tue, 30 Jun 2020 00:46:14: 51000000 INFO @ Tue, 30 Jun 2020 00:46:14: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:46:14: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:46:14: #1 total tags in treatment: 24523233 INFO @ Tue, 30 Jun 2020 00:46:14: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:46:14: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:46:15: #1 tags after filtering in treatment: 20358590 INFO @ Tue, 30 Jun 2020 00:46:15: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 30 Jun 2020 00:46:15: #1 finished! INFO @ Tue, 30 Jun 2020 00:46:15: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:46:15: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:46:16: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 00:46:16: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:46:16: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.10_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 0 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.10_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.10_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.10_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling INFO @ Tue, 30 Jun 2020 00:46:17: 48000000 INFO @ Tue, 30 Jun 2020 00:46:24: 49000000 INFO @ Tue, 30 Jun 2020 00:46:32: 50000000 INFO @ Tue, 30 Jun 2020 00:46:39: 51000000 INFO @ Tue, 30 Jun 2020 00:46:40: #1 tag size is determined as 75 bps INFO @ Tue, 30 Jun 2020 00:46:40: #1 tag size = 75 INFO @ Tue, 30 Jun 2020 00:46:40: #1 total tags in treatment: 24523233 INFO @ Tue, 30 Jun 2020 00:46:40: #1 user defined the maximum tags... INFO @ Tue, 30 Jun 2020 00:46:40: #1 filter out redundant tags at the same location and the same strand by allowing at most 1 tag(s) INFO @ Tue, 30 Jun 2020 00:46:40: #1 tags after filtering in treatment: 20358590 INFO @ Tue, 30 Jun 2020 00:46:40: #1 Redundant rate of treatment: 0.17 INFO @ Tue, 30 Jun 2020 00:46:40: #1 finished! INFO @ Tue, 30 Jun 2020 00:46:40: #2 Build Peak Model... INFO @ Tue, 30 Jun 2020 00:46:40: #2 looking for paired plus/minus strand peaks... INFO @ Tue, 30 Jun 2020 00:46:42: #2 number of paired peaks: 95 WARNING @ Tue, 30 Jun 2020 00:46:42: Too few paired peaks (95) so I can not build the model! Broader your MFOLD range parameter may erase this error. If it still can't build the model, we suggest to use --nomodel and --extsize 147 or other fixed number instead. WARNING @ Tue, 30 Jun 2020 00:46:42: Process for pairing-model is terminated! cut: /home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.20_peaks.narrowPeak: No such file or directory pass1 - making usageList (0 chroms): 1 millis needLargeMem: trying to allocate 0 bytes (limit: 17179869184) rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.20_model.r’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.20_*.xls’: No such file or directory rm: cannot remove ‘/home/okishinya/chipatlas/results/dm3/ERX1818268/ERX1818268.20_peaks.narrowPeak’: No such file or directory CompletedMACS2peakCalling